首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   9141篇
  免费   689篇
  国内免费   3篇
林业   822篇
农学   359篇
基础科学   57篇
  2512篇
综合类   237篇
农作物   444篇
水产渔业   938篇
畜牧兽医   3517篇
园艺   135篇
植物保护   812篇
  2024年   14篇
  2023年   75篇
  2022年   111篇
  2021年   224篇
  2020年   209篇
  2019年   140篇
  2018年   461篇
  2017年   462篇
  2016年   458篇
  2015年   358篇
  2014年   400篇
  2013年   607篇
  2012年   722篇
  2011年   637篇
  2010年   374篇
  2009年   290篇
  2008年   521篇
  2007年   529篇
  2006年   422篇
  2005年   424篇
  2004年   363篇
  2003年   321篇
  2002年   263篇
  2001年   198篇
  2000年   200篇
  1999年   171篇
  1998年   29篇
  1997年   35篇
  1996年   27篇
  1995年   18篇
  1993年   17篇
  1992年   55篇
  1991年   40篇
  1990年   40篇
  1989年   46篇
  1988年   38篇
  1987年   40篇
  1986年   46篇
  1985年   47篇
  1984年   35篇
  1983年   34篇
  1982年   18篇
  1981年   14篇
  1979年   31篇
  1978年   30篇
  1977年   27篇
  1974年   26篇
  1973年   45篇
  1972年   30篇
  1971年   25篇
排序方式: 共有9833条查询结果,搜索用时 15 毫秒
91.
Irrigation scheduling based on the daily historical crop evapotranspiration (ETh) data was theoretically and experimentally assessed for the major soil-grown greenhouse horticultural crops on the Almería coast in order to improve irrigation efficiency. Overall, the simulated seasonal ETh values for different crop cycles from 41 greenhouses were not significantly different from the corresponding values of real-time crop evapotranspiration (ETc). Additionally, for the main greenhouse crops on the Almería coast, the simulated values of the maximum cumulative soil water deficit in each of the 15 consecutive growth cycles (1988–2002) were determined using simple soil-water balances comparing daily ETh and ETc values to schedule irrigation. In most cases, no soil-water deficits affecting greenhouse crop productivity were detected, but the few cases found led us to also assess experimentally the use of ETh for irrigation scheduling of greenhouse horticultural crops. The response of five greenhouse crops to water applications scheduled with daily estimates of ETh and ETc was evaluated in a typical enarenado soil. In tomato, fruit yield did not differ statistically between irrigation treatments, but the spring green bean irrigated using the ETh data presented lower yield than that irrigated using the ETc data. In the remaining experiments, the irrigation-management method based on ETh data was modified to consider the standard deviation of the inter-annual greenhouse reference ET. No differences between irrigation treatments were found for productivity of pepper, zucchini and melon crops.  相似文献   
92.
Summary The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions. Amplification with eight decamer primers generated 149 polymorphic products. Genetic similarities of RAPD profiles were estimated via a coefficient of Jaccard and then the data were processed by cluster analysis (UPGMA). Each genotype was clearly identified and separated from the others. Our results show that RAPD technology is a rapid, precise and sensitive technique for identification of pea genotypes. However, the phylogenetic relationships within the Pisum sativum, which we tested by bootstrap analysis (Wagner parsimony), must be interpreted with caution.  相似文献   
93.
A study was performed in order to evaluate the three-source model (Clumped model) for direct estimation of actual evapotranspiration (ETa) and latent heat flux (LE) over a drip-irrigated Merlot vineyard trained on a vertical shoot positioned system (VSP) under semi-arid conditions. The vineyard, with an average fractional cover of 30%, is located in the Talca Valley, Region del Maule, Chile. The performance of the Clumped model was evaluated using an eddy covariance system during the 2006/2007 and 2007/2008 growing seasons. Results indicate that the Clumped model was able to predict ETa with a root mean square error (RMSE), mean bias error (MBE), and model efficiency (EF) of 0.33, −0.15 mm day−1 and 74%, respectively. Also, the Clumped model simulated the daytime variation of LE with a RMSE of 36 W m−2, MBE of −8 W m−2, and EF of 83%. Major disagreement (underestimated values) between observed and estimated values of ETa was found for clear days after rainfall or foggy days, but underestimated values were less than 10% of the data analysis. The results obtained in this study indicate that the Clumped model could be used to directly estimate vine water requirements for a drip-irrigated vineyard trained on a VSP. However, application of the Clumped model requires a good characterization of the drip-irrigated vineyard architecture.  相似文献   
94.
As a function of the water quality provided by square, circular and oval experimental ponds, the growth, survival and oxygen requirements in epibenthic postlarvae of Farfantepenaeus aztecus were analysed in relation to their routine metabolism and apparent heat increment. Temperature, oxygen concentration, pH and salinity were measured daily in two experimental ponds of each shape. The postlarvae oxygen consumption during two 24‐h cycles, their growth, physiological condition and survival and the productivity in the ponds were estimated. Low values of pH, oxygen concentration and phytobenthos productivity, and reduced postlarvae relative growth and survival were observed in the square ponds. We suggest that the latter results from a deficient water circulation related to the effect of the pond's shape on dissolved oxygen levels and, consequently, on growth and survival. The postlarvae routine metabolism, including feeding, varied between 1.91 and 2.25 mg O2 h?1 g?1 wet weight, whereas the minimum oxygen concentration needed in the ponds is approximately 4.25 mg O2 L?1. These conditions were achieved in the oval ponds concurrent with higher survival and growth values, in which individuals distributed randomly, for which we suggest that oval‐shaped ponds could be the most adequate for the culture of this and other penaeid species.  相似文献   
95.
Formalin is used in shrimp industry to eliminate ectoparasites and as a disinfectant. However, the effect of formalin on shrimp's defense mechanism is unknown. Several biomarkers were used to assess the immune response of juveniles of the shrimp Litopenaeus schmitti exposed to different concentrations of formalin (10, 25 and 50 mg L?1) during 24 and 48 h. Formalin concentrations of 25 and 50 mg L?1 produced a significant reduction in the total haemocyte count at both 24 and 48 h. Peroxidase and phenoloxidase activity did not show variations. The total protein concentration in haemolymph of shrimps was not significantly different between experimental groups. Histological analysis showed hyperplasia in the gills of animals exposed at 10 mg L?1 for 24 h; however, the exposure at 25 and 50 mg L?1 caused necrosis in gills, hepathopancreas and the antennal gland.  相似文献   
96.
A flow cytometric virus-binding assay that directly visualizes the binding and entry of infectious pancreatic necrosis virus (IPNV), infectious haematopoietic necrosis virus (IHNV) and virus haemorrhagic septicaemia virus (VHSV) to several cell lines was established. The highest efficiency of binding was shown by the BF-2 cell line and this was used to study, at the attachment level, the interactions of these cells with salmonid fish viruses in coinfections, and to further determine if the earliest stage of the viral growth cycle could explain the previously described loss of infectivity of IHNV when IPNV is present. Our results demonstrated that IPNV binds to around 88% of cells either in single or dual infections, whereas IHNV attachment always decreased in the presence of any of the other viruses. VHSV binding was not affected by IPNV, but coinfection with IHNV reduced the percentage of virus-binding cells, which suggests competition for viral receptors or co-receptors. Internalization of the adsorbed IHNV was not decreased by coinfection with IPNV, so the hypothetical competence could be restricted to the binding step. Treatment of the cells with antiviral agents, such as amantadine or chloroquine, did not affect the binding of IPNV and VHSV, but reduced IHNV binding by more than 30%. Tributylamine affected viral binding of the three viruses to different degrees and inhibited IPNV or IHNV entry in a large percentage of cells treated for 30 min. Tributylamine also inhibited IHNV cytopathic effects in a dose-dependent manner, decreasing the virus yield by 4 log of the 50% endpoint titre, at 10 mm concentration. IPNV was also inhibited, but at a lower level. The results of this study support the hypothesis that IHNV, in contrast to VHSV or IPNV, is less efficient at completing its growth cycle in cells with a simultaneous infection with IPNV. It can be affected at several stages of viral infection and is more sensitive to the action of antiviral compounds.  相似文献   
97.
Surface properties of fibrous and ground cotton and linen were investigated by inverse gas chromatography (IGC) and the contact angle with different liquids was also measured on fabrics composed of both fibers. Results proved that dispersion component of surface tension (γ s d ) determined by IGC depends not only on the surface energy, but also on several factors influencing the adsorbability of probe molecules on the cellulosic substrates. For cotton samples, the trapping of n-alkanes among waxy molecules in the outer layer of fibers can be presumed. This effect results in larger γ s d for cotton fibers than for linen in spite of the higher wettability of the linen fabrics. Besides the surface energy and trapping effects, the grinding also influences the γ s d values. Specific enthalpy of adsorption (ΔH A ab ) of polar probes could be determined on all linen samples, but only on the ground cotton sample. Lewis acid-base character calculated for linen and ground cotton samples depends on the same effects as the γ s d does. The similar ΔH A ab values of chloroform (acidic) and THF (basic) measured on each of the samples support the conclusion that the surface character is amphoteric, which is also proved by the high ΔH A ab values of the amphoteric ethyl acetate and acetone probes.  相似文献   
98.
The pathogenicity of 10 bacterial isolates was investigated on potato, radish, carrot and beet, including sensitivity and pathogen control efficacy. The isolates were identified by morphological, biochemical and molecular methods. All isolates were pathogenic on radish, carrot, and beet, and were highly virulent on potato. Although the isolates were obtained from different locations in the El Fuerte Valley (Sinaloa, Mexico), they were similar in their morphological, physiological and biochemical characteristics. Sequences of the 16S rRNA gene obtained by PCR were identical for all isolates. These results indicate that the bacterial isolates from potato scabby tissue belong to S. acidiscabies. Furthermore, the effectiveness of fluazinam, both in vitro and under greenhouse and field conditions, represents a possibleoption for chemical control of potato common scab disease. While our results suggest that spraying at seeding is effective in controlling common scab, future studies to combine this treatment with seed dressing before planting will be conducted to determine if there is an increase in disease control.  相似文献   
99.
100.
Barley (Hordeum vulgare) is cultivated on 49.1 million hectares worldwide with 50.2% of the area located in Europe. Powdery mildew, caused by Blumeria graminis f. sp. hordei (Bgh), occurs wherever barley is grown. Cultivar resistance plays an important role in global barley production, especially in parts of Europe where high concentrations of both spring and winter types are grown. The aim of this report was to postulate specific resistance genes in barleys from nine European countries registered in the Czech Republic from 2011 to 2015. Thirty‐five spring cultivars and 27 winter barleys were tested with 56 diverse Bgh isolates. Twenty‐five known resistance genes were postulated, and unknown genes were detected in Sandra, Saturn and Zeppelin. Unidentified specific resistance genes were also present in winter hybrids Hobbit and Wootan. Spring cultivars Arthur and Francin consisted of three and two genotypes, respectively. Resistance gene mlo was present in 26 spring cultivars, and the proportion of cultivars with this gene increased from 62.9% in 2006–2010 to 75.7% in 2011–2015. The gene Mlp1 was identified for the first time in German winter cultivar Saturn. Five spring cultivars registered in Slovakia were included in the tests. All the cultivars that were tested contained one or more specific resistance genes to powdery mildew. Adaptability of the pathogen and possibilities for breeding winter barleys are discussed.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号