Swine Influenza Virus and Association with the Porcine Respiratory Disease Complex in Pig Farms in Southern Brazil          下载免费PDF全文

C. Schmidt  S. P. Cibulski  C. P. Andrade  T. F. Teixeira  A. P. M. Varela  C. M. Scheffer  A. C. Franco  L. L. de Almeida  P. M. Roehe 《Zoonoses and public health》2016,63(3):234-240

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Natural antimicrobials for control of Salmonella Enteritidis in feed and in vitro model of the chicken digestive process     

Geovane R. Oliveira  Willian K. Oliveira  Carla Andrade  Antonio Diego B. Melo  Fernando B. Luciano  Renata E. F. Macedo  Leandro B. Costa 《Journal of animal physiology and animal nutrition》2019,103(3):756-765

This study evaluated the antimicrobial effect of essential oils (EO) and organic acids (OA) against Salmonella Enteritidis in chicken feed and during an in vitro model that mimics the chicken digestive process. The minimal inhibitory concentration (MIC) of allyl isothiocyanate (AITC), carvacrol (CV), propionic acid (PROP) and caproic acid (CAP) were individually determined. Then, based on the MICs of each compound, combinations of EOs and/or OAs were tested to evaluate their synergic antimicrobial effect. The synergic effect of AITC and CAP was the most efficient against the bacterial strain tested. Commercial feed was inoculated with a 5‐strain cocktail of S. Enteritidis and treated with different doses of AITC + CAP to evaluate their effect on the growth/survival of the pathogen. In addition, the simulated digestion model was used to access the antimicrobial effect of AITC + CAP added to the feed towards S. Enteritidis and Lactobacillus plantarum. Synergistic effect was found between AITC (0.065 mM) and CAP (17.5 mM) against S. Enteritidis in chicken feed, where S. Enteritidis was reduced to undetectable levels (<1.00 log CFU/g). AITC (1.95 mM) + CAP (45 mM) also decreased (p < 0.05) the population of S. Enteritidis in the simulated digestion, while the growth of L. plantarum was not affected. Therefore, the addition of AITC + CAP in feed might be a potential natural antimicrobial able to prevent economic losses caused for Salmonella in chicken.  相似文献   

Prevalence of lameness and hoof lesions in all year-round grazing cattle in Brazil     

Tiago Facury Moreira  Rafael Romero Nicolino  Leandro Silva de Andrade  Elias Jorge Facury Filho  Antônio Ultimo de Carvalho 《Tropical animal health and production》2018,50(8):1829-1834

Lameness is a growing concern to the dairy industry worldwide. However, little is known about lameness and its causes in grazing cattle, especially in tropical climates. This study aimed to assess the prevalence of hoof lesions and lameness in dairy herds of all year-round grazing cattle under tropical condition, and to identify the main lesions associated with lameness. We visited 48 farms located in the Minas Gerais state, Brazil, equally divided into four groups based on daily milk production. All lactating cows in the visited farms were locomotion scored, and a representative sample was randomly chosen for hoof inspection. Among the 2267 lactating cows evaluated, 16% were scored as lame and 7% as severely lame. Nearly all cows presented at least one type of hoof lesion, of which heel horn erosion (90%), white line fissure (50%), and digital dermatitis (33%) were the most prevalent. Heel horn erosion was present in all farms and digital dermatitis was present in 96% of the farms. Sole ulcer was observed in a single animal. Additionally, digital dermatitis and white line fissure were correlated to a 2.5 times increase in the odds of a poor mobility score. Collectively, our results demonstrate that digital dermatitis and white line fissure are the main concern and the biggest cause of lameness in grazing cattle under tropical conditions.  相似文献   

Frequency of enterotoxins,toxic shock syndrome toxin-1, and biofilm formation genes in <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> isolates from cows with mastitis in the Northeast of Brazil     

F. N. Costa  N. O. Belo  E. A. Costa  G. I. Andrade  L. S. Pereira  I. A. Carvalho  R. L. Santos 《Tropical animal health and production》2018,50(5):1089-1097

Staphylococcus aureus is among the microorganisms more frequently associated with subclinical bovine mastitis. S. aureus may produce several virulence factors. This study aimed at determining the frequency of virulence factors such as enterotoxins, toxic shock syndrome toxin 1, and ica adhesion genes. In addition, we assessed antimicrobial drug resistance in S. aureus isolated from clinical and subclinical cases of mastitis. A total of 88 cows with clinical or subclinical mastitis were sampled, resulting in 38 S. aureus isolates, from which 25 (65.78%) carried toxin genes, including seb, sec, sed, tst, and icaD adhesion gene. These S. aureus isolates belong to 21 ribotypes and three S. aureus strains belonged to the same ribotype producing ica adhesion gene. Approximately 90% of S. aureus strains obtained in our study demonstrated multiple resistance to different antimicrobial agents. The most efficacious antimicrobial agents against the isolates were gentamicin, amoxicillin, and norfloxacin. Gentamicin was the most efficacious agent inhibiting 78.95% of the S. aureus isolates. The least efficacious were penicillin, streptomycin, and ampicillin. Our results can help in understanding the relationship between virulence factors and subclinical mastitis caused by S. aureus. Further research about diversity of S. aureus isolates and genes responsible for the pathogenicity of subclinical mastitis is essential.  相似文献   

Presumed post‐traumatic ocular chondrosarcoma with intrathoracic metastases in a cat          下载免费PDF全文

Matheus V. L. Moreira  Maria C. de Andrade  Gustavo O. Fulgêncio  Ingeborg M. Langohr  Roselene Ecco 《Veterinary ophthalmology》2018,21(5):535-538

An indoor‐only, 5‐year‐old, spayed female domestic shorthair cat presented for an ophthalmic examination of the left eye. An intraocular tumor with secondary glaucoma and blindness was diagnosed; the globe was enucleated and sent for histopathological examination. Gross examination revealed a solid white mass filling the entire vitreous space and replacing the iris and ciliary body. The lens and retina appeared to be similarly replaced by the neoplasm. Histological examination revealed a complete loss of the internal ocular structures, with a ruptured capsule as the only remnant of the lens within an extensive malignant mesenchymal neoplastic cell proliferation. The cells were polygonal, with well‐defined cytoplasmic borders and abundant weakly basophilic cytoplasm, embedded within the islands of chondroid matrix. No neoplastic invasion of the sclera was apparent. The animal died 6 months after the enucleation due to respiratory distress. Gross examination revealed numerous firm, white to tan nodular masses with smooth to mildly irregular surfaces dispersed throughout the parietal pleura, thoracic surface of the diaphragm, tracheobronchial and mediastinal lymph nodes, pericardium, and lungs. On cross‐section, the neoplastic nodules were solid and variably translucent, resembling hyaline cartilage. Histologically, these nodules were similar to the neoplasm identified earlier in the left globe. Metastasis of post‐traumatic ocular chondrosarcoma has not yet been described in cats. This is therefore believed to be the first report of metastases of this type of neoplasm in cats. This case adds to the limited set of data on the outcome of this type of tumor.  相似文献   

Performance of dual and triple fungicide premixes for managing soybean rust across years and regions in Brazil: A meta-analysis     

Jhonatan P. Barro  Kaique S. Alves  Cláudia V. Godoy  Alfredo R. Dias  Carlos A. Forcelini  Carlos M. Utiamada  Edson R. de Andrade Júnior  Fernando C. Juliatti  Fernando J. Grigolli  Heraldo R. Feksa  Hercules D. Campos  Ione C. P. V. Chaves  Ivan P. Araújo Júnior  João Mauricio T. Roy  José Nunes Júnior  Luana M. R. Belufi  Luciana C. Carneiro  Luís H. C. P. Silva  Marcelo G. Canteri  Marcio M. Goussain Júnior  Marina Senger  Maurício C. Meyer  Moab D. Dias  Mônica A. Müller  Mônica C. Martins  Mônica P. Debortoli  Nédio R. Tormen  Silvânia H. Furlan  Tiago F. Konageski  Valtemir J. Carlin  Wilson S. Venâncio  Emerson M. Del Ponte 《Plant pathology》2021,70(8):1920-1935

Soybean rust in Brazil is currently controlled with several commercial fungicide premixes composed of demethylation inhibitors (EPOXiconazole, CYPRoconazole, PROThioconazole, TEBUconazole), quinone-outside inhibitors (AZOXystrobin, TriFLoXystrobin, PYRAclostrobin, PICOxystrobin), and succinate demethylation inhibitors (BENZovindiflupyr, BIXaFen, FLUXapyroxad). Here, we summarize the performance of eight premixes evaluated in 177 cooperative trials conducted in 46 locations across 10 states from 2015 to 2020. All fungicide treatments were sprayed three times starting at R1/R2. Percentage control (, %), from back-transforming meta-analytic estimates of the log of the ratio, ranged from 56.2% (PICO + CYPR) to 76.8% (BIXF + TFLX + PROT). Estimates of mean yield difference (, kg/ha) between fungicide-treated and untreated plots were greatest for BIXF + TFLX + PROT (1,080) followed by PICO + BENZ (1,010), PYRA + EPOX + FLUX (981.5), AZOX + BENZ (910), TFLX + PROT (891), PICO +TEBU (682), TFLX + CYPR (646), and PICO + CYPR (600). Significant declines in both and in as little as 4 years were detected for AZOX + BENZ (35.3%; 550 kg/ha) and PICO + BENZ (15.5%; 359.8 kg/ha). Variance in was reduced by the inclusion of baseline severity as covariate. In trials where baseline disease was ≥70%, yield was 250 kg/ha greater compared to areas with low baseline disease. Disease control and yield response were generally greater in the south-east, where the frequency of profitable scenarios was 30% higher on average than in the north-west. Results of this meta-analysis are critical for supporting decisions during planning of fungicide programmes.  相似文献   

Early weaning of cuttlefish (Sepia officinalis,L.) with frozen grass shrimp (Palaemonetes varians) from the first day after hatching     

António V Sykes  Rui A Gonçalves  José P Andrade 《Aquaculture Research》2013,44(12):1815-1823

The suitability of early weaning cuttlefish using frozen grass shrimp, and its effects on growth and mortality was determined in a 50‐day experiment. Three food groups were used. One group (C) was fed with live prey until 25th DAH and weaned with frozen prey until the end of the experiment. In the other two groups, weaning started at the fifth DAH (W5) and first DAH (W1), also with frozen diet. Significant differences were found between C and the remaining treatments (P < 0.05) for mean wet weight (MWW), instantaneous growth rate (IGR), biomass (B) and mortality at the 25th and the 50th DAH. Despite the lower growth (~1/3 of the achieved with live food) and higher mortality (3% obtained by C versus 23% in the W1 and W5 groups), C cuttlefish presented a similar weight gain tendency to W1 and W5 groups after weaning. This is the first record of early acceptance (at the first DAH) of frozen food by cuttlefish hatchlings. These differences could be related with digestive enzymes of cuttlefish and prey, changes in nutritional composition and amount of captured prey, etc. Future research should also address the effects of this early weaning on cuttlefish life cycle.  相似文献   

Simulated keratometry and central corneal thickness measured in cats by autokeratometer,ultrasonic pachymeter,and Galilei G6™     

Leticia de Andrade Ramos  Cláudia Valéria Seullner Brandão  Carlos Guillermo Arce  Laura Micheline  Micaella Gordon Gandolfi  Carlos Roberto Padovani 《Veterinary ophthalmology》2023,26(2):128-134

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Practical implications of using non-relational databases to store large genomic data files and novel phenotypes     

Andr Moreira Souza  Rodrigo de Andrade Santos Weigert  Elaine Parros Machado de Sousa  Lucas Tassoni Andrietta  Ricardo Vieira Ventura 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2022,139(1):100-112

The objective of our study was to provide practical directions on the storage of genomic information and novel phenotypes (treated here as unstructured data) using a non-relational database. The MongoDB technology was assessed for this purpose, enabling frequent data transactions involving numerous individuals under genetic evaluation. Our study investigated different genomic (Illumina Final Report, PLINK, 0125, FASTQ, and VCF formats) and phenotypic (including media files) information, using both real and simulated datasets. Advantages of our centralized database concept include the sublinear running time for queries after increasing the number of samples/markers exponentially, in addition to the comprehensive management of distinct data formats while searching for specific genomic regions. A comparison of our non-relational and generic solution, with an existing relational approach (developed for tabular data types using 2 bits to store genotypes), showed reduced importing time to handle 50M SNPs (PLINK format) achieved by the relational schema. Our experimental results also reinforce that data conversion is a costly step required to manage genomic data into both relational and non-relational database systems, and therefore, must be carefully treated for large applications.  相似文献   

Roraimine: a bisbenzylisoquinoline alkaloid from Cissampelos sympodialis roots     

de Lira GA  de Andrade LM  Florêncio KC  da Silva MS  Barbosa-Filho JM  Leitão da-Cunha EV 《Fitoterapia》2002,73(4):356-358

The roots of Cissampelos sympodialis yielded Roraimine (1), a novel bisbenzylisoquinoline alkaloid and liriodenine (2), a known oxoaporphine alkaloid.  相似文献