首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   1757篇
  免费   98篇
  国内免费   3篇
林业   130篇
农学   63篇
基础科学   4篇
  462篇
综合类   123篇
农作物   113篇
水产渔业   94篇
畜牧兽医   704篇
园艺   49篇
植物保护   116篇
  2024年   3篇
  2023年   27篇
  2022年   35篇
  2021年   80篇
  2020年   83篇
  2019年   95篇
  2018年   75篇
  2017年   62篇
  2016年   72篇
  2015年   58篇
  2014年   64篇
  2013年   116篇
  2012年   139篇
  2011年   149篇
  2010年   84篇
  2009年   53篇
  2008年   114篇
  2007年   104篇
  2006年   93篇
  2005年   77篇
  2004年   61篇
  2003年   48篇
  2002年   41篇
  2001年   14篇
  2000年   8篇
  1999年   11篇
  1998年   4篇
  1997年   6篇
  1996年   8篇
  1995年   5篇
  1994年   6篇
  1993年   6篇
  1992年   4篇
  1991年   3篇
  1990年   7篇
  1989年   2篇
  1986年   4篇
  1985年   6篇
  1984年   4篇
  1983年   4篇
  1982年   2篇
  1980年   3篇
  1977年   2篇
  1973年   2篇
  1972年   2篇
  1967年   2篇
  1966年   1篇
  1965年   1篇
  1958年   1篇
  1955年   1篇
排序方式: 共有1858条查询结果,搜索用时 328 毫秒
101.
102.
103.
New aspects of soybean somatic embryogenesis   总被引:4,自引:0,他引:4  
Somatic embryo formation from immature cotyledons was improved in the following ways: by cutting into sections, supplementing culture media with spermine and using solid/liquid/solid type of culture. Cut cotyledons of the eight genotypes examined expressed a higher ability for somatic embryogenesis than whole cotyledons. Of the three polyamines tested, spermine considerably stimulated and putrescine slightly inhibited induction of somatic embryos. The ability of embryoid formation on medium with spermidine depended on the genotype. The solid/liquid/solid type of culture was better than the continuous solid culture. The best nitrogen ion content for the subculture of somatic embryos was 10 mM NH4NO3 and 30 mM KNO3. The possibility of using these modifications in Agrobacterium transformation is discussed.  相似文献   
104.
Summary The capacity of the maize genotype 4c1 to regenerate microcalli and embryos from cultured microspores has been examined by comparing various cold pretreatments and culture media, using microspores and pollen at different stages of development. Viability of cultured cells was tested with FDA and their development was traced with light and fluorescence microscopy using DAPI as a nuclear dye.It was found that a pre-incubation of dissected flowers floating in a liquid nutrient medium at 8°C during 10–14 days was most successful for the induction of cell division. Among the developmental stages tested only the microspores appeared to regenerate. Subculture at 25°C in the same liquid medium, supplemented with 0.1 mg/l TIBA, gave highest rates of microspore division, i.e. up to 70% at 4 to 6 days of culture.All pathways described earlier for maize androgenic embryogenesis were observed within the 4c1 genotype. Symmetric divisions occurred in cultured microspores but most frequently asymmetric divisions lead to the formation of microcalli within 12 days of culture. In at least 60% of all dividing microspores cells were derived from the generative nucleus. Microcalli further developed either into loose or compact calli. Compact calli formed embryo-like structures.Abbreviations DAPI 4,6-diamidino-2-phenylindole - Dicamba 3,6-dichloro-2-methoxy benzoic acid - 2,4D 2,4 dichlorophenoxyacetic acid - FDA fluorescein diacetate - PAA phenylacetic acid - TIBA 2,3,5-triiodobenzoic acid - YP medium Yu-Pei basal salt medium  相似文献   
105.
The PPR‐B gene is responsible for male‐fertility restoration of the Ogura‐type male‐sterile radish plants, and it is located in the complex Rfo locus in the vicinity of similar PPR‐A gene and PPR‐C pseudogene. The aim of this study was to identify PPR‐B alleles and understand the structure of the Rfo locus in radish breeding lines. Five lines of radish with normal male‐fertile cytoplasm were tested. The entire PPR‐B gene was amplified, sequenced and allelic PPR‐B sequences were identified. The results indicated that the maintainer lines 7, 15 and 21 contained a non‐restoring form of PPR‐B protein. A unique PPR‐B was found in lines 24/15 and 31 that are restorer and maintainer lines, respectively. The substitutions might be responsible for the loss of a restoring function of the PPR‐B‐31 allele. Amplification of the PPR‐A/PPR‐B and PPR‐B/PPR‐C intergenic regions allowed to identify rearrangements within Rfo locus. Obtained results confirm the wide allelic variation within the Rfo locus, as well as high genetic complexity of the fertility restoration mechanism in radish.  相似文献   
106.
Average grain weight is a major yield component contributing to its variation, especially in Mediterranean regions where grain weight is frequently exposed to terminal stresses affecting grain growth. Most of the literature agrees that wheat grain growth is hardly limited by the source. However, no source–sink ratios studies seem to have been conducted in the Mediterranean region to determine to what degree wheat grain growth is actually limited by the source in these particular regions. We conducted two field experiments in Catalonia (north-eastern Spain), where an old cultivar (Anza) and a more recently released one (Soissons) were sown in a range of different nitrogen and water availabilities and sowing dates. This was to analyse the degree of source limitation for grain growth. Sink size was modified by removing half of the spikelets c. 10 days after anthesis, virtually doubling the availability of assimilates per grain effectively growing.

Trimming the spikes did not produce significant changes in grain growth rate or duration of grain filling. Consequently, grain weight did not respond noticeably to the reduction in sink demand and any eventual response has been far from representing a strong competition among grains during grain filling.  相似文献   

107.
Book reviewed in this articles: Cereal Grain Protein Improvement. Shivanna, K. R., and B. M. Johri, The Angiosperm Pollen. Structure and Function. Gustafson, J. P., Gene Manipulation in Plant Improvement. Backer, A. W., Manual of Quantitative Genetics. Fishbeck, G., W. Plarre und W. Schuster (Hrag.) , Hoffmann , W., A. Plarre : Lehrbush der Züchtung landwirtschaftlicher Kulturflanzen, Bd. 2, Spezieller Teil, 2. Auflage. Dodds, J. H. (editor and author), Plant Genetic Engineering. Fiechter, A. (managing editor), Advanced in Biochemical Engineering/Biotechnology. Vol. 31. Plant Cell Culture.  相似文献   
108.
109.
110.
This study compares the detection of faecal contamination indicators in irrigation water (25 samples) by two analytical techniques: multiple tube fermentation method (MPN) and Colilert/Enterolert Quanti-Tray system. Our aim was to investigate variations in the test results and determine whether variations could be related to 1) the interval of time between the sample collection and analysis and 2) the manual labour of the operator. Each water sample was analysed at specific intervals of time from the sample collection within 24 h and by three different operators. Statistical analysis was employed to evaluate the results. We have proved that the indices obtained with the multiple tube fermentation method show greater variation than the values obtained with the Colilert and Enterolert systems. Furthermore, some samples, analysed by the MPN method, showed different values, but not statistically significant, in relation to the intervals of time between the sample collection and analysis and in relation to the data obtained by the manual labour of the operators. Values for the same sample varied in respect to legislative limits. The Colilert and Enterolert systems for the detection of indicator bacteria could be a substitute for the standard methods. This procedure could be used for monitoring the quality of irrigation water.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号