Development and application of multiple immunofluorescence staining for diagnostic cytology of canine and feline lymphoma          下载免费PDF全文

Mariko Sawa  Akira Yabuki  Asuka Setoguchi  Osamu Yamato 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(4):580-585

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Enantioseparation of isoxanthohumol in beer by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography     

Kodama S  Yamamoto A  Sato A  Suzuki K  Yamashita T  Kemmei T  Taga A  Hayakawa K 《Journal of agricultural and food chemistry》2007,55(16):6547-6552

Chiral resolution of isoxanthohumol (IX) in beer samples was performed by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography. The optimum running conditions were found to be 20 mM phosphate buffer (pH 7.0) containing 45 mM hydroxypropyl-gamma-cyclodextrin and 100 mM sodium dodecyl sulfate with an effective voltage of +20 kV at 20 degrees C using direct detection at 210, 295, and 370 nm. IX was detected in 12 beer samples and the total levels of (+)- and (-)-IX ranged from 0.15 to 1.4 mg/L. But the amount of xanthohumol (XN) was below the detection limit (0.017 mg/L). Each level of (-)-IX was almost the same as that of (+)-IX, suggesting that IX was a racemic mixture in these beer samples. The racemization of IX in beer could be attributed to the production of a racemic mixture from XN during boiling and to the fact that IX enantiomers were easily interconverted.  相似文献   

Ectomycorrhizal fungal communities of Dipterocarpus alatus seedlings introduced by soil inocula from a natural forest and a plantation     

Tharnrat Kaewgrajang  Uthaiwan Sangwanit  Motoichiro Kodama  Masahide Yamato 《Journal of Forest Research》2014,19(2):260-267

We attempted to introduce ectomycorrhizal (ECM) fungi onto seedlings of Dipterocarpus alatus (Dipterocarpaceae) by soil inocula collected from tree stands of D. alatus. Top soils collected from beneath the trees of D. alatus in a dry evergreen forest and a 15-year-old plantation were inoculated onto germinating seeds of D. alatus. After 7 months of seedling cultivation, ECM fungal communities in the seedlings were investigated based on the sequences of ITS rDNA. The ECM fungi detected were divided into 19 phylotypes by molecular analysis. Most of the phylotypes were identified as ECM fungal taxa, i.e., Clavulina, Laccaria, Lactarius, Tomentella, Pyronemataceae, and Tricholomataceae. Accordingly, we can confirm that soil inoculation is a simple method to induce ECM formation with diverse fungi in pot cultured seedlings, which would be useful for introducing diverse ECM fungi to dipterocarp plantations.  相似文献   

Genomic Reassortants of Pandemic A (H1N1) 2009 Virus and Endemic Porcine H1 and H3 Viruses in Swine in Japan     

Rikio KIRISAWA  Yoshitaka OGASAWARA  Hayato YOSHITAKE  Asuka KODA  Tokujiro FURUYA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1457-1470

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Establishment of an attenuated strain of bovine respiratory syncytial virus for live virus vaccine   总被引：1，自引：0，他引：1  

M Kubota  S Fukuyama  K Kodama  N Sasaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(4):695-703

To develop a live virus vaccine for the prevention of bovine respiratory syncytial (BRS) virus infection in calves, an attempt was made to produce an attenuated virus. The RS-52 strain of BRS virus, isolated from the nasal secretions of a naturally infected calf, was subjected to serial passages in adult hamster lung established (HAL) cells at 30 degrees C and the attenuated rs-52 strain as a live virus vaccine was established. The rs-52 strain multiplied better at 30 degrees C than at 34 or 37 degrees C in HAL cells. The differences in the highest virus titers of this strain between the culture temperature of 30 degrees C and that of 34 or 37 degrees C were more than 2.25 log TCID50. Colostrum-deprived newborn calves and 2 approximately 4 months old calves inoculated with the rs-52 strain manifested no abnormal clinical sings at all. However, all inoculated calves produced serum neutralization antibody. When the colostrum-deprived newborn calves immunized with the rs-52 strain were challenged with the virulent NMK7 strain of BRS virus, they exhibited no pyrexia or other abnormal clinical signs at all. An attempt was made to recover the virus from nasal secretions of these calves, but in vain. On the other hand, a nonimmunized control colostrum-deprived newborn calf developed slight fever, mild cough, and slight serous nasal discharge after challenge exposure. The virus was recovered from nasal secretions of this calf. From these results, it was considered that the rs-52 strain could be used as an attenuated live virus vaccine for prevention of BRS virus infection.  相似文献   

Induction of AIDS in rhesus monkeys by molecularly cloned simian immunodeficiency virus   总被引：92，自引：0，他引：92  

H Kestler  T Kodama  D Ringler  M Marthas  N Pedersen  A Lackner  D Regier  P Sehgal  M Daniel  N King 《Science (New York, N.Y.)》1990,248(4959):1109-1112

Better understanding of the pathogenesis of acquired immunodeficiency syndrome (AIDS) would be greatly facilitated by a relevant animal model that uses molecularly cloned virus of defined sequence to induce the disease. Such a system would also be of great value for AIDS vaccine research. An infectious molecular clone of simian immunodeficiency virus (SIV) was identified that induces AIDS in common rhesus monkeys in a time frame suitable for laboratory investigation. These results provide another strong link in the chain of evidence for the viral etiology of AIDS. More importantly, they define a system for molecular dissection of the determinants of AIDS pathogenesis.  相似文献   

One-way control of FWA imprinting in Arabidopsis endosperm by DNA methylation   总被引：1，自引：0，他引：1  

Kinoshita T  Miura A  Choi Y  Kinoshita Y  Cao X  Jacobsen SE  Fischer RL  Kakutani T 《Science (New York, N.Y.)》2004,303(5657):521-523

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Control of genic DNA methylation by a jmjC domain-containing protein in Arabidopsis thaliana     

Saze H  Shiraishi A  Miura A  Kakutani T 《Science (New York, N.Y.)》2008,319(5862):462-465

Differential cytosine methylation of repeats and genes is important for coordination of genome stability and proper gene expression. Through genetic screen of mutants showing ectopic cytosine methylation in a genic region, we identified a jmjC-domain gene, IBM1 (increase in bonsai methylation 1), in Arabidopsis thaliana. In addition to the ectopic cytosine methylation, the ibm1 mutations induced a variety of developmental phenotypes, which depend on methylation of histone H3 at lysine 9. Paradoxically, the developmental phenotypes of the ibm1 were enhanced by the mutation in the chromatin-remodeling gene DDM1 (decrease in DNA methylation 1), which is necessary for keeping methylation and silencing of repeated heterochromatin loci. Our results demonstrate the importance of chromatin remodeling and histone modifications in the differential epigenetic control of repeats and genes.  相似文献   

Cytokinin oxidase regulates rice grain production   总被引：25，自引：0，他引：25  

Ashikari M  Sakakibara H  Lin S  Yamamoto T  Takashi T  Nishimura A  Angeles ER  Qian Q  Kitano H  Matsuoka M 《Science (New York, N.Y.)》2005,309(5735):741-745

Most agriculturally important traits are regulated by genes known as quantitative trait loci (QTLs) derived from natural allelic variations. We here show that a QTL that increases grain productivity in rice, Gn1a, is a gene for cytokinin oxidase/dehydrogenase (OsCKX2), an enzyme that degrades the phytohormone cytokinin. Reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield. QTL pyramiding to combine loci for grain number and plant height in the same genetic background generated lines exhibiting both beneficial traits. These results provide a strategy for tailormade crop improvement.  相似文献   

Islet regeneration during the reversal of autoimmune diabetes in NOD mice     

Kodama S  Kühtreiber W  Fujimura S  Dale EA  Faustman DL 《Science (New York, N.Y.)》2003,302(5648):1223-1227

Nonobese diabetic (NOD) mice are a model for type 1 diabetes in humans. Treatment of NOD mice with end-stage disease by injection of donor splenocytes and complete Freund's adjuvant eliminates autoimmunity and permanently restores normoglycemia. The return of endogenous insulin secretion is accompanied by the reappearance of pancreatic beta cells. We now show that live donor male or labeled splenocytes administered to diabetic NOD females contain cells that rapidly differentiate into islet and ductal epithelial cells within the pancreas. Treatment with irradiated splenocytes is also followed by islet regeneration, but at a slower rate. The islets generated in both instances are persistent, functional, and apparent in all NOD hosts with permanent disease reversal.  相似文献