Triterpenic and other lipophilic components from industrial cork byproducts   总被引：1，自引：0，他引：1  

Sousa AF  Pinto PC  Silvestre AJ  Pascoal Neto C 《Journal of agricultural and food chemistry》2006,54(18):6888-6893

The detailed chemical composition of the lipophilic extractives of cork and cork byproducts generated throughout industrial processing has been investigated by gas chromatography-mass spectrometry. Triterpenes (cerine, friedeline, and betulinic acid) were the major components detected. Betulinic acid is the main triterpene (11.7 g/kg) identified in industrial cork powder, whereas in black condensates friedeline (95.3 g/kg), betuline (13.1 g/kg), and betulinic acid (12.1 g/kg) are the main triperpenes. Significant fractions of alpha-hydroxy fatty acids (115.1 g/kg) and alpha,omega-dicarboxylic acids (21.2 g/kg) were also detected in black condensate after alkaline hydrolysis. The results demonstrate that these two industrial byproducts can be considered as promising sources of bioactive chemicals or chemical intermediates for the synthesis of polymeric materials.  相似文献   

Genotype-environment interaction in Cordia trichotoma (Vell.)Arráb.Ex Steud.progenies in two different soil conditions     

Wanderley dos Santos  Bruno Marchetti de Souza  Daniele Fernanda Zulian  Gilberto Terra Ribeiro Alves  Jo 《林业研究》2022,33(1):309-319

Investment in silvicultural techniques is notice-ably lacking,especially in breeding programs for non-con-ventional wood species.Studying genotype × environment interaction (G × E) is essential to the development of breed-ing programs.Thus,this study aimed to estimate genetic diversity of and the effects of G × E interaction on two prog-eny tests of Cordia trichotoma,including the estimation of genetic gain and genetic diversity after selection.For the experiment,30 progenies of C.trichotoma were tested at two sites with differing soil textures.Diameter at breast height (1.30 m above soil surface,dbh-cm),total height,diameter at 30 cm from the soil,first branch height,and survival were all monitored for four years.Statistical devi-ance,best linear unbiased estimator,and harmonic mean of relative performance of genetic values (MHPRVG) were all calculated to predict breeding values,estimate genetic parameters,and analyze deviance.All quantified traits varied significantly among progenies by soil type,with greatest variation recorded for genetic variability.Heritability of the progenies led to predictions of genetic gain,ranging from 7.73 to 15.45％,for dbh at four years of age.The calculated decrease in genetic diversity after selection showed that this parameter should be monitored in subsequent breed-ing cycles.G × E was low for all tests.The best-performing progenies proved most stable and best adapted to the differ-ent environmental conditions tested.  相似文献   

Genetic analysis of anthracnose resistance in ‘Pitanga’ dry bean cultivar     

Maria C. Gonçalves‐Vidigal  Ana C. Meirelles  Juliana P. Poletine  Lorenna L. De Sousa  Anelise S. Cruz  Maria P. Nunes  Giselly F. Lacanallo  Pedro S. Vidigal Filho 《Plant Breeding》2012,131(3):423-429

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Comparative study of hematological parameters of Colossoma macropomum anesthetized with benzocaine and eugenol by using different anticoagulants     

Sousa  Andria Gama  Pacheco  Acácio H. B.  Siqueira-Pinto  Gilson A.  dos Reis  Gleika T. J.  Fugimura  Michelle M. S.  Vaz  Luciano J.  Marcusso  Paulo F.  Ramos-Espinoza  Fernando C.  da Silva Claudiano  Gustavo 《Aquaculture International》2021,29(3):977-988

Aquaculture International - The objective of the present study was to evaluate the action of benzocaine and eugenol over anesthesia stages and the use of three anticoagulants (ethylenediamine...  相似文献   

Anomalous Pregnancies during Late Embryonic/Early Foetal Period in High Producing Dairy Cows     

B Serrano  F López-Gatius  RHF Hunter  P Santolaria  I García-Ispierto  G Bech-Sabat  NM de Sousa  JF Beckers  JL Yániz 《Reproduction in domestic animals》2009,44(4):672-676

This study analyses anomalous cases of gestation ending in pregnancy loss during the early foetal period and their effect on progesterone and plasma pregnancy-associated glycoprotein-1 (PAG-1) concentrations. Data derived from a large-scale ultrasound pregnancy diagnosis programme in high producing dairy cows. Over a 3-year period (2004–2007), a very low incidence (0.5%: 15 of 3094) of anomalous pregnancies was recorded. The results revealed that the following anomalies were detected on days 35–41 of gestation in cows carrying singletons with one single corpus luteum: embryo death in eight cows (0.3%); and embryo in the uterine horn contralateral to the corpus luteum in seven cows (0.2%). All these animals suffered pregnancy loss during the early foetal period. In cows carrying dead embryos, no signs of conceptus degeneration were observed on pregnancy diagnosis. Amnion size (approximately 25 mm diameter) and uterine horn fluid contents were estimated to be similar to those of the normal pregnant cows in this period. In the contralateral gestations, live embryos were observed in all ultrasound checks before pregnancy loss. Uterine fluid contents increased in the two cows in which gestation continued for more than a week. In the cases of embryo death but not in those of contralateral gestation, a drop in PAG-1 levels was noted prior to pregnancy loss. Two cows carrying dead embryos increased with time allantoic fluid contents. The PAG-1 values increased with time in one cow bearing a dead embryo (from 2.31 to 6.79 ng/ml) and in two of the contralateral gestations (from 1.66 to 2.33 ng/ml and from 0.39 to 6.79 ng/ml, respectively). Results of this study indicate that the foetal membranes continue to undergo some activity following embryo death, and that contralateral pregnancy may determine failure of the gestation process.  相似文献   

Correlation of five radioimmunoassay systems for measurement of bovine plasma pregnancy-associated glycoprotein concentrations at early pregnancy period     

A. Ayad  N.M. Sousa  J. Sulon  J.L. Hornick  M. Iguer-Ouada  J.F. Beckers   《Research in veterinary science》2009,86(3):377-382

The measurement of serum or plasma PAG concentrations is currently used as a specific method for pregnancy diagnosis in cattle. In this study, the correlation between five radioimmunoassay systems (RIA-497, RIA-706, RIA-780, RIA-809 and RIA–Pool) developed for measurement of PAG concentrations in ruminant species was investigated in plasma from pregnant Friesian Holstein females. Plasma PAG concentrations (ng/mL) measured by different RIA systems were significantly correlated between them ( 0.81; P < 0.001). PAG concentrations increased significantly from Day 21 (n = 27) to 30 (n = 37) after AI by use of all PAG–RIA systems. From Day 30 to 80 after AI, lower PAG concentrations were observed when using the homologous system RIA-497. The addition of several proteinase inhibitors changed neither the non specific binding nor the B₀ binding to the tracer. Our results suggest that all tested PAG–RIA (RIA-497, RIA-706, RIA-780, RIA-809 and RIA–Pool) are highly correlated and can be useful to follow PAG concentrations in samples collected during the first trimester of gestation.  相似文献   

Effects of a lectin-like protein isolated from Acacia farnesiana seeds on phytopathogenic bacterial strains and root-knot nematode     

T. Santi-Gadelha  B.A.M. RochaC.A.A. Gadelha  H.C. SilvaR.E.R. Castellon  F.J.T. GonçalvesD.O. Toyama  M.H. ToyamaA.J.F. de Souza  L.O.S. BeriamJ.L. Martins  P.P. JoazeiroB.S. Cavada 《Pesticide biochemistry and physiology》2012,103(1):15-22

Acacia farnesiana lectin-like protein (AFAL) showed bacterioestatic effects against Xanthomonas axonopodis pv. passiflorae (Gram-negative) and Clavibacter michiganensis michiganensis (Gram-positive), with the latter being more sensitive. This effect is probably due to the ability of AFAL to interact with the bacterial cell wall where we observed that AFAL induced macroscopic change. The maximum bacterial growth inhibition was approximately 78% when incubated with Gram-negative strains, and as high as 92% percent for the Gram-positive one. The antibacterial effect of flavonoids (rutin, quercetin and morin) was also observed using low concentrations against both bacterial strains. Prior incubation of both with AFAL at high concentrations increases the inhibitory effect of flavonoids on bacterial growth. The potential use of AFAL as a control agent against the root-knot nematode Meloidogyne incognita was investigated as well, showing anti-nematode properties involving both egg hatching and motility. In the juvenile second-stage, AFAL showed reduction in larval mobility when measured against a control group. The results suggest that AFAL is effective against M. incognita and could be used as a component of integrated pest management programs. These data also suggest that lectins probably play a role in plant defense not only against invertebrate phytopathogens, herbivores and fungi but also against bacteria.  相似文献   

Pharmacokinetics of tramadol and o-desmethyltramadol in goats after intravenous and oral administration     

de Sousa AB  Santos AC  Schramm SG  Porta V  Górniak SL  Florio JC  de Souza Spinosa H 《Journal of veterinary pharmacology and therapeutics》2008,31(1):45-51

The aim of this trial was to implement a method to obtain a tool for analyses of tramadol and the main metabolite, o-desmethyltramadol (M1), in goat's plasma, and to evaluate the pharmacokinetics of these substances following intravenous (i.v.) and oral (p.o.) administration in female goats. The pharmacokinetics of tramadol and M1 were examined following i.v. or p.o. tramadol administration to six female goats (2 mg/kg). Average retention time was 5.13 min for tramadol and 2.42 min for M1. The calculated parameters for half-life, volume of distribution and total body clearance were 0.94+/-0.34 h, 2.48+/-0.58 L/kg and 2.18+/-0.23 L/kg/h following 2 mg/kg tramadol HCl administered intravenously. The systemic availability was 36.9+/-9.1% and half-life 2.67+/-0.54 h following tramadol 2 mg/kg p.o. M1 had a half-life of 2.89+/-0.43 h following i.v. administration of tramadol. Following p.o., M1 was not detectable.  相似文献   

Natural Benzo/Acetophenones as Leads for New Synthetic Acetophenone Hybrids Containing a 1,2,3-Triazole Ring as Potential Antifouling Agents     

Ana Rita Neves  Daniela Pereira  Catarina Gonalves  Joana Cardoso  Eugnia Pinto  Vitor Vasconcelos  Madalena Pinto  Emília Sousa  Joana R. Almeida  Honorina Cidade  Marta Correia-da-Silva 《Marine drugs》2021,19(12)

Marine biofouling is a natural process that represents major economic, environmental, and health concerns. Some booster biocides have been used in biofouling control, however, they were found to accumulate in environmental compartments, showing negative effects on marine organisms. Therefore, it is urgent to develop new eco-friendly alternatives. Phenyl ketones, such as benzophenones and acetophenones, have been described as modulators of several biological activities, including antifouling activity (AF). In this work, acetophenones were combined with other chemical substrates through a 1,2,3-triazole ring, a strategy commonly used in Medicinal Chemistry. In our approach, a library of 14 new acetophenone–triazole hybrids was obtained through the copper(I)-catalyzed alkyne-azide cycloaddition “click” reaction. All of the synthesized compounds were evaluated against the settlement of a representative macrofouling species, Mytilus galloprovincialis, as well as on biofilm-forming marine microorganisms, including bacteria and fungi. The growth of the microalgae Navicula sp. was also evaluated after exposure to the most promising compounds. While compounds 6a, 7a, and 9a caused significant inhibition of the settlement of mussel larvae, compounds 3b, 4b, and 7b were able to inhibit Roseobacter litoralis bacterial biofilm growth. Interestingly, acetophenone 7a displayed activity against both mussel larvae and the microalgae Navicula sp., suggesting a complementary action of this compound against macro- and microfouling species. The most potent compounds (6a, 7a, and 9a) also showed to be less toxic to the non-target species Artemia salina than the biocide Econea^®. Regarding both AF potency and ecotoxicity activity evaluation, acetophenones 7a and 9a were put forward in this work as promising eco-friendly AF agents.  相似文献   

Comparative analysis of main on-farm irrigation systems in Portugal   总被引：2，自引：0，他引：2  

Pedro Le  o de Sousa  Luí  s L. Silva  Ricardo P. Serralheiro 《Agricultural Water Management》1999,40(2-3):341-351

Traditional irrigation systems still cover a large area of irrigated lands in Portugal. The needs for competitiveness leads to the gradual abandon of traditional systems, resulting in social and economic impacts. Pressurised on-farm irrigation systems provide labour savings but imply important investments. Surface irrigation does not require expensive equipment and is low energy and low labour consuming, if modern systems are implemented. Flexible-pipe, gated-pipe, surge-flow valve and cablegation are the main equipment issues available to Portuguese farmers for modern surface irrigation practice. Conditions for application of these systems have been studied relative to different soil and topographic conditions. Plane land, either horizontal or sloped and undulated fields can be irrigated by surface systems, if a convenient design is accomplished. The adequacy of the irrigation systems has been evaluated and a cost-benefit study was carried out. A case study of a situation with undulated land, representative of South Portugal, is presented in this paper, comparing surface irrigation issues and a sprinkler system. Conclusions show a particular economic interest on adopting surge-valve, gated-pipe and cablegation.  相似文献