Evaluation of real-time RT-PCR assay for the routine intra vitam diagnosis of classical swine fever     

Depner K  Hoffmann B  Beer M 《Veterinary microbiology》2007,121(3-4):338-343

The aim of the study was to evaluate the diagnostic quality of the real-time RT-PCR assay described by Hoffmann et al. [Hoffmann, B., Beer, M., Schelp, C., Schirrmeier, H., Depner, K., 2005. Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever. J. Virol. Methods 130, 36-44] for the routine intra vitam diagnosis of classical swine fever (CSF). We compared the assay with conventional diagnostic methods by using defined diagnostic material from an animal experiment with pigs showing different clinical forms of CSF. Compared to virus isolation and antigen ELISA an enhanced sensitivity of the real-time RT-PCR could be shown. We were able to detect all infected pigs regardless of the clinical course of CSF. CSF infection was detected already during the incubation period, during the entire clinical phase as well as at the beginning of convalescence when the first antibodies were detected and no virus could be isolated any more. In most cases, positive PCR results were obtained 2 days earlier than with virus isolation and 2-4 days earlier than with the antigen ELISA.  相似文献   

Plasma interleukin-6 response is predictive for severity and mortality in canine systemic inflammatory response syndrome and sepsis   总被引：4，自引：0，他引：4  

Rau S  Kohn B  Richter C  Fenske N  Küchenhoff H  Hartmann K  Härtle S  Kaspers B  Hirschberger J 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(3):253-260

BACKGROUND: Sepsis is still a major cause of death in both human and veterinary medicine. Early diagnosis is essential for appropriate treatment. Identification of patients at risk for developing sepsis is already possible in human medicine through the measurement of plasma interleukin-6 (IL-6) levels. In veterinary medicine, however, this has been investigated only in canine experimental models. OBJECTIVES: The purpose of this study was to measure IL-6 plasma levels in dogs with naturally occurring systemic inflammatory response syndrome (SIRS) and sepsis and to analyze the value of IL-6 as a predictive parameter for severity and mortality. METHODS: Included in the study were 79 dogs that had been admitted to the small animal clinics of Munich and Berlin from July 2004 to July 2005 and that satisfied the diagnostic criteria for SIRS and sepsis as defined using established parameters. Measurement of plasma IL-6 levels on days 0, 1, and 2 was performed by the use of a colorimetric bioassay based on IL-6-dependent cell growth. RESULTS: Septic foci were identified in 43 patients (septic group), and 36 patients were enrolled in the SIRS group. The frequency of positive blood cultures was 11%. The overall mortality rate was 48%. Higher plasma IL-6 levels on the day of admission were significantly correlated with a more severe degree of disease, increased mortality rate, and earlier fatality. CONCLUSIONS: Plasma IL-6 concentration is predictive of outcome in canine SIRS and sepsis and may be a valuable laboratory parameter for assessing critically ill dogs.  相似文献   

Root renewal of sugar beet as a mechanism of P uptake efficiency     

Bernd Steingrobe 《植物养料与土壤学杂志》2001,164(5):533-539

Sugar beet (Beta vulgaris L.) was grown in two different long‐term P fertilization experiments on a sandy and a loamy soil. The P supply levels of the soils were ”︁low”, ”︁sufficient”, and ”︁high”, according to the German recommendation scheme. The low P level decreased shoot and storage root yield only on the loam soil, where the recovery of the P‐deficient plants after a drought period was slower than at a sufficient P supply. The size of the living root system, as determined by a conventional auger sampling method, peaked at early July and decreased until harvest on the sandy soil without any influence of the P level. On loam, the living root systems were more constant and larger at P shortage. Total root production, as determined by the ingrowth core method, was about 120 km m^—2 in the well P supplied loam treatments and 200 km m^—2 at P deficiency, which was 3—4 times and 5 times higher than the average size of the living root systems, respectively. Hence, a rapid root renewal took place. On sand, where no P deficiency occurred, total root production was not different between the P supply levels but higher than in the well‐supplied loam treatments. Modelling P uptake revealed that this root turnover and the concomitant better exploitation of the soil facilitates P uptake at a low P level in soil, but is of no advantage at a sufficient P supply. The increase of root production at P shortage increased calculated P uptake by 25% compared to a calculation with the ”︁usual” root production at a sufficient supply.  相似文献   

Kinetics of the release of dissolved organic matter (DOM) from air‐dried and pre‐moistened soil material     

Gabriele Ellen Schaumann  Christian Siewert  Bernd Marschner 《植物养料与土壤学杂志》2000,163(1):1-5

In this study, the kinetics of soil organic matter (SOM) dissolution from soil samples in different states of moisture was investigated, using a continuous extraction method. The investigation distinguished three processes of SOM dissolution. They include an initial, fast process (probably hydrophilic dissolved organic matter) and two slow, rate limited processes, which probably correspond to hydrophobic dissolved organic matter (DOM). The second process indicates a slow, continuous release of DOM, whereas the third process is determined by a power law. The rate of the third process strongly depends on temperature and state of moisture. It is diffusion limited, with the diffusion control probably being located in the solid soil organic matter. This was explained by a gel structure, which slowly forms in the hydrating SOM and allows diffusion of mobile particles of SOM. The results show the importance of considering the moisture state of SOM for the kinetics of DOM dissolution.  相似文献   

Averting the baiji syndrome: conserving habitat for critically endangered dolphins in Eastern Taiwan Strait     

Peter S. Ross  Sarah Z. Dungan  Samuel K. Hung  Thomas A. Jefferson  Christina Macfarquhar  William F. Perrin  Kimberly N. Riehl  Elisabeth Slooten  John Tsai  John Y. Wang  Bradley N. White  Bernd Würsig  Shih Chu Yang  Randall R. Reeves 《水产资源保护:海洋与淡水生态系统》2010,20(6):685-694

• 1. Numbering no more than 100 individuals and facing many threats, the geographically isolated Eastern Taiwan Strait population of Indo‐Pacific humpback dolphins (Sousa chinensis) is in peril. The estuarine and coastal waters of central‐western Taiwan have historically provided prime habitat for these dolphins, but environmental conditions today bear little resemblance to what they were in the past.
• 2. The humpback dolphins must share their habitat with thousands of fishing vessels and numerous factories built upon thousands of hectares of reclaimed land.
• 3. They are exposed to chemicals and sewage released from adjacent terrestrial activities. Noise and disturbance associated with construction, vessel traffic and military activities are features of everyday life for these animals.
• 4. Measures to slow the pace of habitat deterioration and reduce the many risks to the dolphins are urgently needed. As one practical step in this direction, this paper describes the habitat needs of these small cetaceans so that decision makers will be better equipped to define ‘priority habitat’ and implement much needed protection measures under the terms of local legislation.
• 5. The preferred habitat of these dolphins in Taiwan consists of shallow (<30 m), near‐shore marine waters with regular freshwater inputs.
• 6. For such a small, isolated and threatened population, ‘priority habitat’ should not be limited to areas of particularly intensive dolphin use or high dolphin density, but rather it should encompass the entire area where the animals have been observed (their current ‘habitat’), as well as additional coastal areas with similar bio‐physical features (‘suitable habitat’). Such a precautionary approach is warranted because the loss of only a few individuals could have serious population‐level consequences.
• 7. While conventional socio‐economic analysis might suggest that implementing protection measures over an area stretching ～350 km north–south along Taiwan's west coast and ～3 km out to sea would be too ‘costly’, the loss of this charismatic species from Taiwan's waters would send a troubling message regarding our collective ability to reconcile human activities with environmental sustainability. Copyright © 2010 John Wiley & Sons, Ltd.

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Measurement and simulation of herbicide transport in macroporous soils     

C. Florian Stange  Bernd Diekkrüger  Henning Nordmeyer 《Pest management science》1998,52(3):241-250

Lysimeter experiments were carried out to study pesticide transport through macroporous soils. In order to differentiate between the effects of soil structure and chemical behaviour, the leaching experiments were conducted using disturbed and undisturbed soil samples. Two herbicides with different sorption behaviours, and bromide as tracer were applied. The results were used to validate a dynamic simulation model which considers bypass flow in macropores. The simulation results show that the model is able to reproduce the soil suction within the soil as well as the spatial distribution of bromide and the herbicides. The continuity of the macropores is most important for the efficiency of bypass flow. The results indicate that cultivation practices like ploughing significantly influence the temporal and spatial distribution of the macropores. © 1998 SCI.  相似文献   

4‐Phenylphenalenones as a template for new photodynamic compounds against Mycosphaerella fijiensis          下载免费PDF全文

William Hidalgo  Marisol Cano  Manuela Arbelaez  Edwin Zarrazola  Jesús Gil  Bernd Schneider  Felipe Otálvaro 《Pest management science》2016,72(4):796-800

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Certainties and uncertainties about the life cycle of the Périgord black truffle (<Emphasis Type="Italic">Tuber melanosporum</Emphasis> Vittad.)     

François Le Tacon  Andrea Rubini  Claude Murat  Claudia Riccioni  Christophe Robin  Beatrice Belfiori  Bernd Zeller  Herminia De la Varga  Emila Akroume  Aurélie Deveau  Francis Martin  Francesco Paolocci 《Annals of Forest Science》2016,73(1):105-117

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Cadmium Uptake and Translocation of Tomato in Response to Simulated Irrigation Water Containing Elevated Concentrations of Cadmium and Zinc in Clayey Soil     

Mamoun A. Gharaibeh  Ammar A. Albalasmeh  Bernd Marschner  Yasmeen Saleem 《Water, air, and soil pollution》2016,227(5):133

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Chlorogenic acid,a metabolite identified by untargeted metabolome analysis in resistant tomatoes,inhibits the colonization by Alternaria alternata by inhibiting alternariol biosynthesis     

Edyta Wojciechowska  Christoph H. Weinert  Björn Egert  Bernhard Trierweiler  Markus Schmidt-Heydt  Bernd Horneburg  Simone Graeff-Hönninger  Sabine E. Kulling  Rolf Geisen 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(4):735-747

Tomato fruits can be contaminated by saprophytic strains of Alternaria alternata which is the reason for the frequent occurrence of Alternaria toxins like alternariol, alternariol monomethylether or tenuazonic acid in these types of products. It was shown earlier that alternariol is a colonization factor for tomatoes. In the current analysis two different tomato genotypes were analysed by untargeted comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS). This analysis revealed clear differences in the metabolic profiles which were paralleled by differences in resistance towards Alternaria colonization. One of the genotypes was more resistant against A. alternata infection and contained high amounts of chlorogenic acid in contrast to the other genotype which was sensitive against infection. In in vitro analysis, chlorogenic acid reduced alternariol biosynthesis during the first days of growth of A. alternata. Expression analysis of the alternariol polyketide synthase gene, a key gene in the biosynthesis of alternariol, also revealed a temporal reduction in its expression in the first phases of growth. However by chromatographic analysis it could be demonstrated that chlorogenic acid was degraded over time. This degradation leads to a relief of inhibition resulting in an only temporal inhibition of alternariol biosynthesis. In vivo colonization experiments revealed that chlorogenic acid reduces colonization of tomatoes by A. alternata in a concentration dependent manner, which however is partly counteracted by the addition of alterariol.  相似文献