The effect of 3 resuscitative fluid therapies on hemostasis as measured by rotational thromboelastometry in dogs          下载免费PDF全文

Sunita Seshia  M. Casey Gaunt  Beverly A. Kidney  Marion L. Jackson 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(1):38-44

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Effect of different mini‐volume colloid centrifugation configurations on flow cytometrically sorted sperm recovery efficiency and quality using a computer‐assisted semen analyzer          下载免费PDF全文

LB Ferré  JL Chitwood  C Fresno  HH Ortega  ME Kjelland  PJ Ross 《Reproduction in domestic animals》2018,53(1):26-33

Straws of sex‐sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 10⁶ sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of using sex‐sorted sperm under in vitro fertilization conditions, the selection of an appropriate sperm separation technique is essential. In this study, the effect of using different silane‐coated silica colloid dilutions and layering configurations during centrifugation of sex‐sorted sperm was examined over an extended period of incubation time. Sperm recovery and viability after centrifugation using the colloid separation technique were measured along with several sperm motility parameters using CASA. For this purpose, frozen and thawed sex‐sorted sperm samples were centrifuged using mini‐volume single‐layer (40%, 60% and 80%) and mini‐volume two‐layer (45%/90%, 40%/80% and 30%/60%) separation configurations using PureSperm^®. A single layer of 40% PureSperm^® recovered significantly more sex‐sorted sperm (78.07% ± 2.28%) followed by a single layer of 80% PureSperm^® (68.43% ± 2.33%). The lowest sperm recovery was obtained using a two‐layer PureSperm^® dilution of 45%/90% (47.57% ± 2.33%). Single‐layer centrifugation recovered more sorted sperm (68.67% ± 1.74%) than two layer (53.74% ± 1.74%) (p < .0001). A single layer of 80% PureSperm^® exhibited the highest sorted sperm viability (72.01% ± 2.90%) after centrifugation (p < .05). The mini‐volume single layer of 80% PureSperm^® was determined to be an effective alternative to a two‐layer centrifugation configuration for sex‐sorted sperm selection. In addition, single‐layer colloid dilution of 80% performed either as well as or significantly outperformed the other treatments, as well as the control, with regard to motility (MOT) for all time periods of analysis.  相似文献   

Comparison of DRIS and alternative nutrient diagnostic methods for soybean     

R. B. Beverly 《Journal of plant nutrition》2013,36(8):901-920

The Diagnosis and Recommendation Integrated System (DRIS) provides advantages over the Sufficiency Range (SR) approach to diagnosing the nutrient status of soybean (Glycine max L.). However, a number of modifications to DRIS have been proposed, including the use of only one method for calculating nutrient indices, and incorporating nutrient concentrations. In previous research, the author found that derivation and interpretation of DRIS diagnoses could be simplified by: 1) using a logarithmic transformation of nutrient ratio data; 2) using population parameters rather than high‐yield subpopulation values; 3) using a single index calculation method; and, 4) incorporating a measure of the probability of yield response to a treatment. Diagnoses by the SR approach, DRIS, three revisions of DRIS, and two new concentration‐based diagnostic methods were compared using diagnostic norms derived from a data base of over 4000 soybean tissue analyses and yield observations. Virtually all diagnoses of the most limiting nutrient were the same, except that the SR method did not make diagnoses on 5 week‐old samples. Traditional DRIS diagnoses were the least conservative, indicating only the order in which N, P and K would likely limit yield. Revised methods, particularly the concentration‐based methods, indicated fewer limiting nutrients, and agreed well with the SR method. Yields in the test data set increased with nearly every nutrient application, so the least conservative DRIS showed the greatest yield advantage. No diagnostic method consistently identified the nutrient causing the greatest yield response as most limiting.  相似文献   

Genetic variation affects de novo translocation frequency     

Kato T  Inagaki H  Yamada K  Kogo H  Ohye T  Kowa H  Nagaoka K  Taniguchi M  Emanuel BS  Kurahashi H 《Science (New York, N.Y.)》2006,311(5763):971

Translocation is one of the most frequently occurring human chromosomal aberrations. The constitutional t(11;22)(q23;q11), which is the only known recurrent non-Robertsonian translocation, represents a good model for studying translocations in humans. Here we demonstrate polymorphisms of the palindromic sequence at the t(11;22) breakpoint that affect the frequency of de novo translocations in sperm from normal males. A typical allele consists of a perfect palindrome, producing ~10-5 de novo t(11;22) translocations. Alleles with an asymmetric center do not form the t(11;22). Our data show the importance of genome sequence on chromosomal rearrangements, a class of human mutation that is thought to be random.  相似文献   

Porcine circovirus-2 associated disease in swine in Ontario (2004 to 2005)     

Carman S  McEwen B  DeLay J  van Dreumel T  Lusis P  Cai H  Fairles J 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(8):761-762

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Density‐dependent effects of eastern Kamchatka pink salmon (Oncorhynchus gorbuscha) and Japanese chum salmon (O. keta) on age‐specific growth of western Alaska chum salmon     

Tessa J. Frost  Ellen M. Yasumiishi  Beverly A. Agler  Milo D. Adkison  Megan V. McPhee 《Fisheries Oceanography》2021,30(1):99-109

Hatcheries release >4.5 billion juvenile Pacific salmon (Oncorhynchus spp.) into the North Pacific Ocean annually, raising concerns about competition with wild salmon populations. We used retrospective scale analysis to investigate how the growth of chum salmon (O. keta) from western Alaska is affected by the abundance of chum salmon from Japanese hatcheries and wild pink salmon (O. gorbuscha) from the Russian Far East. Over nearly five decades, the growth of Kuskokwim River chum salmon was negatively correlated with the abundance of Japanese hatchery chum salmon after accounting for the effects of sex and spring/summer sea‐surface temperature in the Bering Sea. An effect of wild eastern Kamchatka pink salmon abundance on the growth of Kuskokwim River salmon was detectable but modest compared to the intraspecific competitive effect. A decrease in Japanese hatchery chum salmon releases in 2011–2013 was not associated with increased growth of Bering Sea chum salmon. However, the abundance of wild chum salmon from the Russian Far East increased during that time, possibly obscuring reduced competition with hatchery chum salmon. Our results support previous evidence that chum salmon are affected by intraspecific competition, and to a lesser extent interspecific competition, in the North Pacific, underscoring that the effects of salmon hatchery production transcend national boundaries.  相似文献   

Nested multiplex RT-PCR for detection and differentiation of West Nile virus and eastern equine encephalomyelitis virus in brain tissues.     

Donna J Johnson  Eileen N Ostlund  Beverly J Schmitt 《Journal of veterinary diagnostic investigation》2003,15(5):488-493

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Hyperostotic polyarthropathy in a rabbit - a suspected case of chronic hypervitaminosis A from a diet of carrots     

JL FRATER 《Australian veterinary journal》2001,79(9):608-611

Chronic hypervitaminosis A can occur in many species after excessive dietary intake of Vitamin A (retinol). The most common presentation of chronic hypervitaminosis A is a polyarthropathy with hyperostosis and ankylosis of various joints. This case report describes a probable case of naturally occurring hypervitaminosis A-induced polyarthropathy in a rabbit after chronic ingestion of a diet made up almost exclusively of carrots. Carrots do not contain retinol, but are rich in provitamin A (or beta-carotene). Rabbits are unique in that they can convert 100% of dietary beta-carotene into retinol. A syndrome of naturally occurring hypervitaminosis A-induced polyarthropathy has not been described in a rabbit before.  相似文献   

Local Effect of the Corpus Luteum on Ovarian Follicular Functional and Morphological Features in the Goat     

F González  M Batista  F Cabrera  P Calero  JL Alabart  & A Gracia 《Reproduction in domestic animals》2001,36(3-4):147-151

Ovaries of unilaterally ovulated goats (n=21) were used to study follicular morphological features (proportion and degree of atresia, oestradiol production in vitro and progesterone production of granulosa cells in culture). Follicles were dissected out and classified as small (1–2 mm), medium (2–4 mm) and large (>4 mm). Morphological and physiological features were compared in each size class between ovaries bearing and not bearing corpora lutea (CLO and NCLO, respectively). Within the same size class, there was no difference in proportion or in degree of atresia, between CLO and NCLO. A significant effect of follicular size on oestradiol production in vitro was detected, but no effect of the corpus luteum was found. Finally, progesterone production of granulosa cells in culture was significantly higher in CLO than in NCLO after 24 h (p < 0.05) and also after 90 h (p < 0.01) of culture. This higher progesterone production by CLO granulosa cells in culture could be explained by local influence of the corpus luteum stimulating the steroidogenic activity but not aromatase activity. Further studies are needed to clarify possible factors and pathways for this local effect of the corpus luteum upon follicular physiology.  相似文献   

Cervical diskospondylitis in two horses     

CM COLBOURNE  SL RAIDAL  JV YOVICH  JMcC HOWELL  JL RICHARDSON 《Australian veterinary journal》1997,75(7):477-479

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