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111.
Gonadotrophin releasing hormone was administered as an intravenous bolus injection into four boars and four ovariectomized sows. Radioimmunoassay of concentrations of gonadotrophin releasing hormone in blood collected periodically after injection indicated a biexponential decline suggesting a rapid distribution to the extracellular fluid and a slower elimination by metabolism. A mean half-life value of 2.12 +/- 0.95 (SD) minutes was calculated for the first component and of 13.15 +/- 2.55 minutes was calculated for the second component of the decline in gonadotrophin releasing hormone concentrations. No significant difference was detected between boars and sows for half-life value of either component. In the four boars, luteinizing hormone values reached a peak in plasma 20 minutes after injection and that of testosterone at 90 minutes after gonadotrophin releasing hormone treatment.  相似文献   
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Over the last three decades, farming systems in Europe and Australia have seen a decline in legume plantings, leading to reduced soil carbon and fertility, and an increase in plant disease, reliance on industrial nitrogen fertilizer and herbicides. In Australia, one reason for this decline has been the movement towards sowing crops and forages into dry soil, before the opening rains, as a consequence of climate variability. This practice predicates against the survival of rhizobial inoculants, and hence generates uncertainty about legume performance. The research reported here was initiated to improve the robustness of a specific forage legume/rhizobia symbiosis to increase nitrogen fixation in low pH, infertile soils. Rhizobial strains (Rhizobium leguminosarum biovar viciae) from Pisum sativum L. were sourced from acid soils in southern Italy and southern Australia. Strains were evaluated for N fixation on the forage legumes P. sativum, Vicia sativa and Vicia villosa, then for survival and persistence in acid soils (pHCa 4.6). Fourteen of the strains produced a higher percentage of nitrogen derived from the atmosphere (%Ndfa) compared to commercial comparator strain SU303 (<78%). Twenty‐two strains survived sufficiently into the second season to form more nodules than SU303, which only achieved 3% of plants nodulated. Elite strains WSM4643 and WSM4645 produced six times more nodulated plants than SU303 and had significantly higher saprophytic competence in acid soil. These strains have the ability to optimize symbiotic associations with field peas and vetch in soils with low fertility, carbon and pH that are restrictive to the current commercial strain SU303.  相似文献   
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We evaluated radio frequency (RF) dielectric heating for eradication of pinewood nematodes (PWN) in infested wood. Thirteen temperatures were tested (from ambient to 70 °C) on small wood samples (2.5 × 3.8 × 0.64 cm) to determine the minimum lethal temperature (100 % mortality), which was 56 °C [based on infra-red (IR) thermal images data 55.5–57.4 °C] with a 1 min hold time. We also used thermal probes inside the wood to confirm that temperatures were ≥56 °C. Thirty additional samples were tested bracketing the minimum lethal temperature using 54, 56 and 58 °C with additional replications to produce the minimum sample size equivalent of 100 % mortality of at least 93,616 nematodes to satisfy the Probit 9 efficacy requirement. This minimum lethal temperature was further verified by treating infested large wood blocks (10.2 × 10.2 × 25.4 cm). All samples that met or exceeded the 56 °C lethal temperature for the required 1 min hold time (as measured by probes inserted in the wood and on the wood surface by IR) produced 100 % mortality. The sample size required to show Probit 9 efficacy was also satisfied. This study supports the consideration of RF in addition to microwave (MW) dielectric heating as alternative treatments of wood packaging material for inclusion in ISPM No. 15, provided the treatment delivers the target lethal temperature throughout the profile of the material in industrial scale operations.  相似文献   
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Although bovine respiratory disease complex (BRDC) is common in post-weaning cattle, BRDC prediction models are seldom analyzed. The objectives of this study were to assess the ability to predict cumulative cohort-level BRDC morbidity using on-arrival risk factors and to evaluate whether or not adding BRDC risk classification and daily BRDC morbidity and mortality data to the models enhanced their predictive ability. Retrospective cohort-level and individual animal health data were used to create mixed negative binomial regression (MNBR) models for predicting cumulative risk of BRDC morbidity. Logistic regression models were used to illustrate that the percentage of correctly (within |5%| of actual) classified cohorts increased across days, but the effect of day was modified by arrival weight, arrival month, and feedlot. Cattle arriving in April had the highest (77%) number of lots correctly classified at arrival and cattle arriving in December had the lowest (28%). Classification accuracy at arrival varied according to initial weight, ranging from 17% (< 182 kg) to 91% (> 409 kg). Predictive accuracy of the models improved from 64% at arrival to 74% at 8 days on feed (DOF) when risk code was known compared to 56% accuracy at arrival and 69% at 8 DOF when risk classification was not known. The results of this study demonstrate how the predictive ability of models can be improved by utilizing more refined data on the prior history of cohorts, thus making these models more useful to operators of commercial feedlots.  相似文献   
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Until recently, most contemporary ecologists have ignored or diminished anecdotal historical accounts and anthropologists' reports about aboriginal fire in the Great Basin. Literature review shows that Indians practiced regular use of fire for many purposes, including the obvious reasons of increasing the availability of desired plants, maintaining habitats for animals used as food, and driving game during hunts. Historical accounts of prehistoric anthropogenic firing, inferences from fire-scar data, and data regarding annual production capability of representative sagebrush (Artemisia spp.)-perennial grass ecological sites indicate that prehistoric conditions were neither fuel- nor ignition-limited. According to many sources, this “active management” by Indians was widespread, significant, and more common than lightning-caused fires, resulting in mosaic vegetation patterns that subsequently moderated the behavior of “natural fires.” This interaction between Indian-burning and lightning fires may have strongly influenced the pre-Euro-American settlement vegetation of the Great Basin. At the very least, the landscape was a patchwork of areas altered by aboriginal people and areas shaped primarily by bio-physical processes. Based on this prehistoric precedent, current historically unprecedented conditions (fuel load and exotic weed invasion threats), and predicted climate change, contemporary active management of sagebrush-perennial grass communities is paramount. Restoration measures should be scientifically based and tailored to achieve ecological resilience and functionality in specific sites. Prescribed fire is not always ecologically appropriate or judicious, especially in Wyoming big sagebrush (A. tridentata spp. wyomingensis) communities, so managers should consider using other alternatives where an intentional low severity distubance is deemed necessary. Properly planned active management would disrupt fuel continuity for lighthning fires, ensure ecological process and successional integrity, and benefit multiple uses on a landscape scale.  相似文献   
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