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Hermesch DR Thomson DU Loneragan GH Renter DR White BJ 《American journal of veterinary research》2008,69(9):1229-1234
OBJECTIVE: To determine effects of vaccination with siderophore receptor and porin (SRP) proteins derived from Salmonella enterica serotype Newport on milk production, somatic cell count, and shedding of Salmonella organisms in female dairy cattle. ANIMALS: 180 female Holsteins. PROCEDURES: Cattle were randomly assigned to receive Salmonella Newport SRP vaccine or control solution. Vaccine or control solution was injected 45 to 60 days before parturition, and cattle received a second dose 14 to 21 days before parturition. Milk production was monitored for the first 90 days of lactation. Feces for isolation of Salmonella and blood samples for detection of antibodies against Salmonella Newport were collected at day of first injection and at days 7 to 14 and 28 to 35 of lactation. RESULTS: Cattle inoculated with Salmonella Newport vaccine produced significantly more milk (1.14 kg/d), compared with cattle injected with the control solution. Cattle administered the vaccine had significantly higher concentrations of circulating antibody against Salmonella Newport SRP proteins at 7 to 14 days and 28 to 35 days of lactation. Salmonella Newport was not recovered; however, Salmonella enterica serotype Agona was recovered from 31 (20.3%) cattle, but likelihood of recovery did not differ significantly between vaccinates and control cattle. CONCLUSIONS AND CLINICAL RELEVANCE: Administration of a vaccine against Salmonella Newport SRP proteins to healthy dairy cattle prior to parturition increased milk production, even in cattle without detectable shedding of Salmonella Newport or clinical signs of salmonellosis. Additional research is needed to clarify the mechanisms by which productivity was improved. 相似文献
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Shrub recruitment in arid and semiarid regions often occurs in pulses controlled by specific weather events. Previous research suggested that Wyoming sagebrush in Wyoming is no exception. We examined four species/subspecies of sagebrush in Nevada, in 2009 and 2010, to discover if evidence of recruitment pulses was contained in the annual growth-ring records. Sagebrush species and subspecies occur on a wide variety of ecological sites that require different management strategies. Species included black sagebrush (Artemisia nova A. Nelson), Wyoming big sagebrush (Artemisia tridentata subsp. wyomingensis Beetle & Young), Lahontan sagebrush (Artemisia arbuscula subsp. longicaulis Winward & McArthur), and low sagebrush (Artemisia arbuscula Nutt. ssp. arbuscula). Eighty stem sections were collected from each of 24 stands (6 stands per species or subspecies) at different geographic locations along east-west or north-south gradients where each species or subspecies naturally occurred. Annual growth-ring analysis was used to determine the year of establishment and the relationship between recruitment and weather events. Results indicated stand ages and locations were different (P > 0.001) among species and subspecies, and years of recruitment were strongly correlated with local and hemispheric weather patterns. Linear and multiple regressions modeled recruitment pulses for all four species. Weather-based predictor variables indicated complex interactions between recruitment and climatic controls. Pacific Decadal Oscillation (PDO) index variables were prominent predictors for all four species at their associated sites. Other important local weather variables included total annual precipitation the year before recruitment, the year of recruitment, and the year following recruitment. In Nevada and the Great Basin, it is imperative that successful sagebrush seeding technologies are discovered and implemented. Ecological restoration and postfire rehabilitation methods should be timed correctly with respect to precipitation patterns (positive phase PDO) and/or designed to mimic conditions responsible for natural sagebrush recruitment. 相似文献
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Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine. 总被引:1,自引:0,他引:1
Kathy T Kurth Tsungda Hsu Eric R Snook Eileen L Thacker Brad J Thacker F Chris Minion 《Journal of veterinary diagnostic investigation》2002,14(6):463-469
A number of polymerase chain reaction (PCR)-based diagnostic tests have been developed for Mycoplasma hyopneumoniae, including one from this research group. This report presents further development, optimization, and standardization of a nested PCR test. Detection sensitivity was 1 fg of M. hyopneumoniae chromosomal DNA (approximately 1 organism). This exceeded the sensitivity of or compared favorably with other published PCR tests. Polymerase chain reaction primers to porcine beta2-microglobulin were included as internal controls for amplifiable chromosomal DNA from porcine samples. To standardize the test, a number of samples from experimentally infected pigs, including nasal, tonsil, tracheobronchial swabs, lung tissue, bronchial alveolar lavage (BAL) fluid, and tracheobronchial brush samples, were examined by PCR. Samples obtained from BAL fluid and tracheobronchial sites were most predictive of infection, whereas nasal swabs and lung tissue were not reliable indicators of experimentally induced infection. In conclusion, the nested PCR developed for this study was found to be a highly sensitive and specific diagnostic tool for M. hyopneumoniae, but the enhanced sensitivity may be unnecessary if the proper sites are sampled. 相似文献
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