全文获取类型
收费全文 | 118篇 |
免费 | 13篇 |
专业分类
林业 | 5篇 |
农学 | 1篇 |
24篇 | |
综合类 | 13篇 |
农作物 | 4篇 |
水产渔业 | 2篇 |
畜牧兽医 | 77篇 |
园艺 | 1篇 |
植物保护 | 4篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 2篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 8篇 |
2015年 | 2篇 |
2014年 | 9篇 |
2013年 | 6篇 |
2012年 | 11篇 |
2011年 | 10篇 |
2010年 | 11篇 |
2009年 | 8篇 |
2008年 | 11篇 |
2007年 | 6篇 |
2006年 | 3篇 |
2005年 | 4篇 |
2004年 | 5篇 |
2003年 | 2篇 |
2002年 | 4篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1990年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有131条查询结果,搜索用时 15 毫秒
61.
Belshe R Franchini G Girard MP Gotch F Kaleebu P Marthas ML McChesney MB McCullough R Mhalu F Salmon-Ceron D Sekaly RP van Rompay K Verrier B Wahren B Weissenbacher M 《Science (New York, N.Y.)》2004,305(5681):177-80; author reply 177-80
62.
63.
DAA Oliveira CG Aleixo SA Prado RK Luz NLAF Sarmento EA Teixeira MR Luz EM Turra 《Reproduction in domestic animals》2013,48(6):1049-1055
Only a few studies have described hormonal treatments for induction of synchronicity and gamete collection in Nile tilapia (Oreochromis niloticus), both important for assortative matings in breeding programmes and essential for polyploidy technologies. In this study, we compared the effectiveness of carp pituitary extract (CPE), Nile tilapia pituitary extract (TPE), human chorionic gonadotropin (hCG) and gonadotropin‐releasing hormone (GnRH) protocols on the induction of spawning and egg production in Nile tilapia. Among the hormonal treatments analysed, only hCG was effective for producing viable gametes for in vitro fertilization. To verify the viability of this hormonal treatment, hCG was tested using different doses (1000, 2000, 3000, 4000 and 5000 IU/kg) in a large number of females (208 animals) from two Nile tilapia lines. The results indicated that hCG doses between 1000 and 5000 IU/kg could be used to induce final oocyte maturation in Nile tilapia with collection of stripped oocytes. This is the first study to report differential reproductive responses to hormonal treatment between tilapia lines: line 1 was more efficient at producing eggs and post‐hatching larvae after hCG induction than line 2. In conclusion, we demonstrated that the hCG protocol may be applied on a large scale to induce final oocyte maturation in Nile tilapia. The development of a protocol for in vitro fertilization in Nile tilapia may aid in breeding programmes and biotechnological assays for the development of genetically modified lines of Nile tilapia. 相似文献
64.
Simon Egli François Ayer Martina Peter Britta Eilmann Andreas Rigling 《Annals of Forest Science》2010,67(5):509-509
65.
Böttcher J Boje J Janowetz B Alex M König P Hagg M Götz F Renner K Otterbein C Mages J Meier N Wittkowski G 《Veterinary microbiology》2012,159(3-4):282-290
A voluntary marker-independent Bovine Herpesvirus 1 (BoHV1) eradication program started in 1986; in 1998 it changed to a compulsory one. Certification of free regions in European member states is based on Article 10 of directive 64/432/EEC. According to this rule Bavaria is listed as free of BoHV1 since October 2011. Surveillance of BoHV1-free dairy cattle farms is currently performed with quarterly bulk-milk testing. Non-negative bulk-milk results must be confirmed by blood tests in cattle older than nine months. An increased regional rate of non-negative bulk-milk samples and the subsequent detection of epidemiologically non-feasible singleton BoHV1-reactors by analysis of blood were observed at the final stage of eradication in southwest Bavaria. Nineteen case farms (734 animals) defined by singleton reactors born at least two years after certification of the farms as BoHV1-free, 23 negative control (NC) farms (NC I: 321 animals) from the same region, 11 NC-farms (NC II: 423 animals) from an already-certified Article 10 region in northeast Bavaria and two BoHV1-infected farms (264 animals) were analysed using BoHV1-, BoHV2- and Feline Herpesvirus 1 (FeHV1)-neutralisation tests (NTs), and three commercially available ELISAs supplied by Idexx Laboratories, B.V., The Netherlands: the CHEKIT? Trachitest 2nd Gen. test for milk or serum (Trachitest), Herdchek? gB- (gB-ELISA) and Herdchek? gE-ELISA (gE-ELISA). Significantly increased levels of BoHV2 antibodies were observed on case farms compared to NC I or II farms. Additionally, reactivity by gB-ELISA and the Trachitest was significantly increased for animals with BoHV2 neutralising antibodies. Singleton BoHV1-reactors tested negative by gE-ELISA even if an elevated cut-off of 0.95±0.05 was applied. At this cut-off, the gE-ELISA was as sensitive and specific as the gB-ELISA. Comparative titration of milk samples from seropositive animals from a BoHV1-infected dairy cattle farm and from singleton BoHV1-reactors performed in CHEKIT? Trachitest 2nd Gen. Milk revealed that the slopes of both groups were distinct; therefore, optimised cut-offs for bulk-milk testing to exclude singleton BoHV1-reactors are proposed. 相似文献
66.
Wood BA Troyer RM Terwee JA Vandewoude S 《Veterinary immunology and immunopathology》2012,145(3-4):604-610
We recently described the development and validation of a highly sensitive and specific microsphere immunoassay capable of simultaneously quantifying three domestic cat cytokines in tissue culture supernatant. Here we describe the modification of this assay to measure interferon gamma (IFNγ), interleukin (IL)-10 and IL-12/IL-23 p40 (IL-12/23) in domestic cat plasma, report values obtained from plasma collected after feline immunodeficiency virus (FIV) exposure, and compare plasma concentrations to blood cell mRNA expression. The validated quantitation limits of this assay are 31–1000 pg/ml for IFNγ, 63–2000 pg/ml for IL-10, and 20–625 pg/ml for IL-12/23. Plasma cytokine levels from domestic cats infected with pathogenic and/or apathogenic FIV were determined at 3–4 and 7–8 weeks post-infection. IL-12/23 was elevated (p < 0.05) during acute infection with both FIV strains in two similar studies, conducted five years apart in different feline cohorts (n = 44 total animals). IL-12/23 concentrations ranged from 377 to 1904 pg/ml in naïve cats and 552 to 3460 pg/ml in infected cats. In contrast, the majority of plasma samples had IFNγ and IL-10 concentrations below the lowest standard tested. The inability to consistently detect levels of IFNγ and IL-10 in plasma, despite the fact that mRNA changes were detected, suggests that these cytokines may be secreted and/or cleared in a more highly regulated manner than IL-12/23, or perhaps exert local effects under tighter peripheral constraints and/or at a lower effective concentration. 相似文献
67.
68.
In this study, salt‐induced changes in the growth rate of maize (Zea mays L.) were investigated during the first phase of salt stress. Leaf growth was reduced in the presence of 100 mM NaCl, and effects were more pronounced for the salt‐sensitive cv. Pioneer 3906 in comparison to the hybrid SR03. While hydrolytic activity of plasma membrane remained unaffected, H+‐pumping activity was reduced by 47% in Pioneer 3906, but was unchanged in SR03. Changes in apoplastic pH were detected by ratiometric fluorescence microscopy using the fluorescent dye fluorescein isothiocyanate‐dextran (50 mM). Pioneer 3906 responded with an increase of 0.2 pH units in contrast to SR03 for which no apoplastic alkalization was found. With respect to the hypothesis that the apoplastic pH is influenced by salinity, it is suggested that salt resistance is partly achieved due to efficient H+‐ATPase proton pumping, which results in cell‐wall acidification and loosening. 相似文献
69.
Effects of meiotic inhibitors and gonadotrophins on porcine oocytes in vitro maturation,fertilization and development
下载免费PDF全文
![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
MA Gil A Nohalez CA Martinez JR Ake‐Villanueva F Centurion‐Castro C Maside C Cuello J Roca I Parrilla EA Martinez 《Reproduction in domestic animals》2017,52(5):873-880
This study evaluated the effect of three reversible meiotic inhibitors (MINs) and their interaction with gonadotrophins (Gns) on the meiotic maturation and developmental competence of porcine oocytes. In experiment 1, the oocytes were matured for 22 hr in the presence or absence of dbcAMP (1 mM), cycloheximide (7 μM) or cilostamide (20 μM) with or without Gns, and for an additional 22 hr in the absence of MINs and Gns. At 22 hr of maturation, regardless of the presence of Gns, a higher proportion (p < .001) of oocytes cultured in the presence of MINs were effectively arrested at the germinal vesicle stage compared with the oocytes cultured without MINs. At 44 hr of maturation, the proportion of oocytes that reached MII was higher (p < .05) in groups with Gns compared with groups without Gns. In experiment 2, oocytes that were matured as in experiment 1 were inseminated and cultured for 7 days to evaluate fertilization parameters and blastocyst formation. Only oocytes from the dbcAMP + Gns group had higher (p < .05) efficiency of fertilization compared with the other treatment groups. The presence of dbcAMP during maturation also increased (p < .05) blastocyst formation and efficiency of blastocyst formation in both the presence and absence of Gns. These results indicate that the interaction of Gns with the tested MINs improved meiotic progression. In addition, regardless of supplementation with Gns, the presence of dbcAMP during the first maturation period increased and even doubled the capacity of oocytes to develop to the blastocyst stage. 相似文献
70.
Challenges in Pig Artificial Insemination 总被引:4,自引:0,他引:4
J Roca JM Vázquez MA Gil C Cuello I Parrilla EA Martínez 《Reproduction in domestic animals》2006,41(S2):43-53