Oestrogen and Progesterone Receptor Gene Expression in Canine Oocytes and Cumulus Cells Throughout the Oestrous Cycle     

JSA Gonçalves  CI Vannucchi  FC Braga  FF Paula-Lopes  MP Milazzotto  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2009,44(S2):239-242

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Acid–Base Changes in Canine Neonates Following Normal Birth or Dystocia     

CF Lúcio  LCG Silva  JA Rodrigues  GAL Veiga  CI Vannucchi 《Reproduction in domestic animals》2009,44(S2):208-210

There are limited data concerning blood gas parameters in neonatal dogs. Knowledge of the normal physiology may facilitate effective therapeutic intervention and potentially reduce neonatal mortality. This study examined acid–base parameters in pups born at normal parturition (n = 27) compared with those born after obstetrical assistance or caesarean operation (n = 13) and those born following oxytocin (OXY) administration for treatment of uterine inertia (n = 11). Pups were subjected to an objective scoring method of neonatal health adapted from use in humans (the Apgar score) at birth and again at 5 and 60 min after birth. Venous blood samples were collected at 5 and 60 min after birth for evaluation of blood gas parameters. At birth, all pups had low Apgar scores and a mixed acidosis. The base excess was lowest for pups delivered after OXY administration. The Apgar score improved for all pups after 5 min of birth and there was an improvement in carbon dioxide tension, base excess and venous blood pH at 1 h, although in all pups a metabolic acidosis persisted. These data provide an important insight into neonatal physiology and the variability of blood gas parameters in pups born at normal and abnormal parturition and provide the basis for clinical decision making following dystocia.  相似文献   

Evaluation of Efficacy and Safety of Zinc Gluconate Associated with Dimethyl Sulphoxide for Sexually Mature Canine Males Chemical Neutering     

FRM Soto  WG Viana  GCB Mucciolo  FYM Hosomi  CI Vannucchi  CP Mazzei  AR Eyherabide  C De Fátima Lúcio  RA Dias  SS De Azevedo 《Reproduction in domestic animals》2009,44(6):927-931

The aim of this research was to evaluate the efficacy of zinc gluconate associated with dimethyl sulphoxide (DMSO) for chemical neutering in canine males. Fifteen sexually mature male dogs were divided in two groups, named control and treated. An injection was administered to both testicles, at a concentration of 26.2 mg zinc gluconate per ml and 0.5% DMSO in the treated group (11 dogs). The control group was given injections of saline solution (four dogs). Clinical examination and blood collection for a haemogram were done both before and after drug injection. There were 12 spermograms performed to analyse sperm motility, sperm vigour, ejaculate volume, testicle size, pathology and sperm concentrations. Libido was also measured. An ultrasound examination and histopathology were performed at the end of the experiment. Dogs’ libido after chemical injection was reduced by over 50%. The spermogram analysis showed final mean results of 14.54% for sperm motility, 0.72 of sperm vigour and 37 150 per million spermatozoa per millilitre, values considered below the necessary levels at which fertilization can occur. Ultrasound and histopathology analyses of testicles for the treated group revealed more intense injuries when compared with the control group, with compromised testicular parenchyma and a decrease of germ cell number leading to total atrophy, indicating that the treatment reduced the fertilizing potential of male dogs, promoting a possible subfertile status.  相似文献   

猪链球菌重组GDH蛋白间接ELISA检测方法的建立   总被引：1，自引：0，他引：1  

徐敏  王淑杰  蔡雪辉  刘永刚  石文达  武佳斌  李丽琴 《中国预防兽医学报》2009,31(8)

为建立猪链球菌快速诊断方法,本研究以纯化的原核表达的猪链球菌谷氨酸脱氢酶蛋白(GDH)为抗原,建立了检测猪链球菌抗体的间接ELISA诊断方法.实验确定了最佳抗原包被浓度为6 μg/mL,待检血清最佳稀释倍数为1:100,其作用时间为60 min,兔抗猪酶标抗体的最佳稀释倍数为1:2 000,其作用时间为60 min;判定标准为S/P值≥0.210时判为阳性,S/P值≤0.166时判为阴性,介于两者之间的判为可疑.实验结果表明该方法特异性、敏感性和重复性均较好,适用于猪链球菌所有亚型的抗体检测,为猪链球菌的流行病学调查提供了一种简便快速的血清学检测方法.  相似文献   

辐照处理对干制海兔杀菌效果及其营养成分的影响     

冯敏  王玲  杨萍  刘春泉  唐玉新  林家彬  朱佳廷  王德宁 《核农学报》2010,24(5):982-986

以干制海兔为原料,研究辐照对其杀菌效果及营养成分的影响。结果表明：辐照对干制海兔杀菌效果明显,4kGy剂量辐照后,菌落总数由初始的107cfu/g降低到<103cfu/g,霉菌和金黄色葡萄球菌<10cfu/g,大肠菌群<10MPN/100g,辐照前后均未检出沙门氏菌。从统计分析的结果看,虽然辐照组中蛋白质、脂肪及大部分不饱和脂肪酸的含量都显著高于或低于CK中的含量,但和水分、碳水化合物、VA等一样,它们的含量与辐照剂量没有显著的线性关系,即它们的含量不因辐照剂量的增加显著增加或减少;辐照对灰分、氨基酸（除胱氨酸和脯氨酸）及VB2和VB3的含量没有明显的影响,对VE的影响显著,当辐照剂量为2kGy时,VE的含量高于CK中的含量,而剂量继续增大时其含量却不断减少且都低于CK中的含量。  相似文献   

西藏玛旁雍错流域湖泊面积变化及成因分析   总被引：5，自引：0，他引：5  

拉巴  边多  次珍  拉巴卓玛  陈涛 《干旱区研究》2012,29(6):992-996

利用1975年地形图、1990年、1999-2009年TM卫星遥感影像和近35 a(1975-2009年)气温、降水、积雪日数、蒸发量等气候资料,分析研究了位于西藏阿里地区南部普兰县境内玛旁雍错、拉昂错湖泊面积变化对气候变化的响应。结果表明：在过去35 a里玛旁雍错、拉昂错面积先减少后增加,总体呈减小趋势,到2009年玛旁雍错面积为415.44 km²,拉昂错面积为261.36 km²。与1975年相比2个湖泊面积分别减少了1.56 km²和11.01 km²;受气候变暖的影响,流域附近的冰川面积也在加速退缩。对1975-2009年普兰县气象资料统计分析可知,降水量减少是导致湖泊面积缩小的主要原因,蒸发量不显著的增加及雪冰消融也是近几年湖泊面积波动变化的原因之一。  相似文献   

压缩工艺对毛白杨脲醛树脂胶浸注改性效果的影响研究     

杜兰星  孙照斌  蔡家斌  王新建 《林业机械与木工设备》2012,(1):23-27

对毛白杨木材浸注脲醛树脂胶制备压缩改性木材中的主要影响因素及相关工艺参数进行初步探索与试验,并在实验结果基础上讨论了各因素对制作工艺及其性能的影响。结果表明:①影响板材性能由主到次因素的顺序为压缩率-热压时间-热压温度;②在试验参数范围内较好的工艺参数为热压温度140℃、热压时间20min、压缩率50%;③在试验参数范围内热压时间对试件增重率、含水率、树脂留存率影响显著,而热压温度对试件增重率影响显著,压缩率对试件密度、变形回复率、吸水厚度膨胀率影响显著。  相似文献   

基于K-means聚类和图像分割的紫色土发生层边界识别     

杨凯  慈恩  刘彬  陈洋洋  谢宇 《土壤学报》2024,61(4)

土壤学始于对土壤剖面及其形态特征的观察,剖面发生层的划分与发生层边界特征的描述是土壤调查的基础。实地划分发生层需要丰富的土壤学实践经验,存在主观和缺乏统一划分标准的问题。以紫色土剖面图像为研究对象,采用K-means聚类和图像分割技术,结合图像的颜色特征（CIE Lab色彩空间）和纹理特征（Entropy）识别紫色土剖面发生层边界,并与实地划分的结果进行比较。结果表明：（1）CIE Lab色彩空间的a、b通道和Entropy纹理特征,可以划分出供试剖面的主要发生层（A、B、C）和基岩（R）;（2）聚类识别的发生层数量和发生层深度与实地识别的结果基本一致;除Z2剖面的C层和Z6剖面的Ap层聚类识别与实地识别的发生层下边界深度相差较大（分别为13 cm和8 cm）外,其余发生层下边界深度相差均在3 cm以内;（3）聚类识别的发生层边界形状更为不规则,明显度更为模糊。K-means聚类和图像分割技术实现了紫色土剖面发生层边界的客观识别,可为土壤剖面智能辨识系统的开发提供科学参考。  相似文献   

鹿茸X型胶原基因的全长cDNA克隆及序列分析     

冯海华  宋德光  赵丽红  慈鑫鑫  何凤艳  岳占碰  宋斯伟  时琳  邓旭明 《中国兽医学报》2008,28(4):442-445

采用RT-PCR和RACE技术,从鹿茸软骨细胞总RNA中扩增克隆了梅花鹿X型胶原（collagen X,colX）全长cDNA序列,并进行了序列特性分析。序列分析结果表明,梅花鹿colX全长cDNA序列为3135nt,其中5′非编码区96nt、3′非编码区1014nt和2022nt的开放阅读框编码674个氨基酸的鹿colX前体蛋白,与牛、猪、犬、人类和小鼠等脊椎动物colX氨基酸序列之间的同源性超过82%。按该基因氨基酸序列构建了进化树,揭示其与牛基因更加同源。  相似文献   

西藏主要农区农田土壤肥力状况研究   总被引：4，自引：0，他引：4       下载免费PDF全文

钟国辉  田发益  旺姆  张红锋  刘翠花  次白 《土壤》2005,37(5):523-529

西藏由于其特殊的地理位置、气候类型和地质历史以及耕作习惯,使其具有特殊的土壤特征。在2002~2004年间,我们对西藏主要农业区的林芝、拉萨、日喀则、山南等4地(市)的农田土壤进行了采样分析,分析结果显示:4地(市)农田土壤酸碱性由弱酸性至弱碱性(pH6.5~9.0);养分的基本状况是有机质含量在5~50g/kg之间,变幅较大,N素少(全N0.2~2g/kg,速效N20~80mg/kg),P素缺(全P0.22~1.42g/kg,速效P1~133mg/kg),K素(全K1.9~12.2g/kg,速效K80~1290mg/kg)和微量元素比较丰富(Cu10~443mg/kg,Zn47~266mg/kg,Fe1880~7550mg/kg,Mo0.2~6.6mg/kg,Mn196~736mg/kg,B0.5~138mg/kg)。  相似文献