首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   175篇
  免费   11篇
  国内免费   12篇
林业   23篇
农学   10篇
基础科学   3篇
  30篇
综合类   29篇
农作物   27篇
水产渔业   5篇
畜牧兽医   57篇
园艺   2篇
植物保护   12篇
  2023年   2篇
  2022年   7篇
  2021年   6篇
  2020年   4篇
  2019年   3篇
  2018年   9篇
  2017年   10篇
  2016年   8篇
  2015年   4篇
  2014年   10篇
  2013年   11篇
  2012年   21篇
  2011年   19篇
  2010年   4篇
  2009年   8篇
  2008年   8篇
  2007年   11篇
  2006年   7篇
  2005年   10篇
  2004年   4篇
  2003年   4篇
  2002年   3篇
  2001年   5篇
  2000年   2篇
  1999年   4篇
  1998年   5篇
  1997年   2篇
  1992年   1篇
  1989年   1篇
  1988年   2篇
  1987年   1篇
  1986年   1篇
  1981年   1篇
排序方式: 共有198条查询结果,搜索用时 31 毫秒
11.
本文对新兽药制剂林可霉素水溶性剂,用人工感染发病的方法进行了药效学研究。  相似文献   
12.
Change in potential natural vegetation (PNV) distribution associated with climate change due to the doubling atmospheric carbon dioxide (2×CO2) was estimated with a global natural vegetation mapping system based on the modified Kira scheme to the globe and the continents. With an input of widely-distributed global climate data, the system interpolates data onto a 1° latitude by 1° longitude grid over the globe, generates estimates of vegetation type, and produces a composite PNV map. The input climate data corresponding to the 1×CO2 and 2×CO2 consists of observations prior to AD 1958 at 2,001 weather stations worldwide and the 2×CO2 simulation output from the Japan Meteorological Research Institue's General Circulation Model, respectively. As a result of the simulated global warming, the vegetation zones expanded mostly from the tropics toward the poles. PNV area changed by 6.98 billion (G) ha of the total land area (15.04 Gha) and potential forest area corresponding to the closed forest and open forest (woodland) reached 9.74 Gha with the increase of 1.29 Gha. The potential forest area in Europe had obvious advantages to the climate change accompanied with the increase of actual forest area. Although the actual forest area has decreased in North America and Asia, the potential forest area in these continents also benefitted from the climate change. In the end, the remaining continents tended to bear the brunt of the climate change.  相似文献   
13.
分析德化县竹业开发现状和存在问题,指出发展竹业的有利条件,并提出加快竹业发展的思路、目标和对策,以推进竹业产业化进程。  相似文献   
14.
Oleoylchitosans (O-chitosans), with different molecular masses and degrees of substitution (DS), were synthesized by reacting chitosan with oleoyl chloride. The FT-IR suggested the formation of an amide linkage between amino groups of chitosan and carboxyl groups of oleic acid. The viscosity of O-chitosan sharply increased with the increase of concentration, whereas that of unmodified chitosan rose only slightly. This increase was stronger as the increase of hydrophobicity (DS) and molecular mass of the polymer. The critical aggregation concentration (CAC) of O-chitosans with DS 5, 11, and 27% were 79.43, 31.6, 10 mg/L, respectively, and the CAC of samples with molecular masses of 20, 38, 300, and 1100 kDa were 50.1, 74.93, 125.9, and 630.9 mg/L, respectively. All of the O-chitosans could reduce surface tension slightly. Nanoparticles were prepared using an O/W emulsification method. Mean diameters of the polymeric amphiphilic nanoparticles of O-chitosans with DS 5 and 11% were around 327.4 and 275.3 nm, respectively.  相似文献   
15.
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.  相似文献   
16.
Although the major capsid proteins (MCPs) of lymphocystis disease virus (LCDV) have been characterized, little is known about the host-derived immune response to MCPs and other LCDV antigenic proteins. To identify antigenic proteins of LCDV that could be used as vaccine candidates in olive flounder, Paralichthys olivaceus, we analysed the viral proteins responsible for its virulence by applying immuno-proteomics. LCDV proteins were separated by one-dimensional gel electrophoresis, transferred to polyvinylidene difluoride membrane, and probed with homogeneous P. olivaceus antisera elicited by LCDV natural infection and vaccination with formalin-killed LCDV. Four immune-reactive proteins were obtained at 68-, 51-, 41- and 21 kDa using antisera collected from natural infection while two proteins at 51- and 21 kDa exhibited response to antisera from vaccinated fish, indicating that the latter two proteins have vaccine potential. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nanoelectrospray MS/MS, the 51 and 21 kDa proteins were identified as MCP and an unknown protein, respectively.  相似文献   
17.
海冰水盐分浓度及灌溉次数对土壤水分和棉花产量的影响   总被引:2,自引:0,他引:2  
为了探讨环渤海地区海冰水灌溉技术,于2008年采用微区试验,研究了海冰水不同盐分浓度(1、3、5、7、9 g/L)和不同灌溉次数对土壤水分及棉花产量的影响。结果表明,采用海冰水灌溉能明显提高土壤水分含量,尤其以播种前灌溉和苗期灌溉效果最佳,当每次灌溉量为450 m3/hm2时,与不灌溉相比,土壤含水率增幅为22.85%~31.32%,可以有效缓解棉田春季旱情。不同处理的棉花,以盐分质量浓度1 g/L海冰水、灌溉3次的处理的产量、平均果枝数以及单颗棉桃质量3项指标均为最高,比不灌溉处理增产38.63%;其次为盐分质量浓度3 g/L海冰水灌溉2次的处理,增产率为 29.8%。随灌溉水矿化度增加,产量呈现递减趋势,盐分质量浓度为9 g/L、灌溉3次的处理与不灌溉处理相比出现显著减产,减产率为28.48%。综合考虑海冰水长期灌溉效应及海冰脱盐技术,建议采用盐分质量浓度3 g/L海冰水进行棉田灌溉,降雨正常年份以播种前和苗期2次灌溉为宜,每次灌水量450 m3/hm2,并要注意配合必要的灌溉和排水措施。  相似文献   
18.
Choi YJ  Yoon JH  Cha SW  Lee SG 《Fitoterapia》2011,82(6):911-919
Ginsenoside Rh1 has been reported to possess antiallergic and anti-inflammatory activities, but its effects on monocytes remain to be determined. Herein, we investigated the effects of Rh1 on the expression of MCP-1 and CCR2, activation of MAPK signaling, and chemotaxis of monocytes. Treatment of Rh1 decreased the levels of MCP-1 and CCR2 and the expression of VLA5 and activated β1 integrin on the cell surface, and attenuated the phosphorylation of MAPKs. Based on these results, the inhibitory effects of Rh1 on monocyte function should be regarded as a promising new anti-inflammatory response with a potential therapeutic role against inflammation-dependent diseases.  相似文献   
19.
本文以详细的气象资料为依据,对区划指标进行了计算分析,明确了陕西省风能资源的时间、地域分布状况以及风能资源量,对陕西省风能资源进行了区划,并进行了分区论述。在风能可利用地区实地考查的基础上,就陕西省风能资源的开发利用,提出了具体意见和措施。  相似文献   
20.
Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell model. Lutein bioaccessibility was low, and approximately 75% of total C. vulgaris lutein was not micellized during the digestion process but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during C. vulgaris digestion. C. vulgaris was microfluidized at a pressure exceeding 10000 psi, and the cell surface disruption was visualized by scanning electron microscopy. The mean C. vulgaris particle size was reduced from 3.56 to 0.35 μm with the microfluidization treatment. C. vulgaris microfluidization at 20000 psi was three times more efficient for aqueous lutein micelles production as compared with untreated C. vulgaris, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization. C. vulgaris lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for improving lutein bioaccessibility from C. vulgaris during food processing.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号