Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages   总被引：3，自引：0，他引：3  

Kim MK  Hossein MS  Oh HJ  Fibrianto HY  Jang G  Kim HJ  Hong SG  Park JE  Kang SK  Lee BC 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):627-632

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.  相似文献   

浮床种植草坪草对富营养化水体的净化作用研究   总被引：2，自引：0，他引：2  

牛昶  韩烈保  侯国华  常智慧 《草业科学》2007,24(10):90-93

采用浮床种植草坪草技术,研究了高羊茅Festuca arundinacea、匍匐翦股颖Agrostis stolonifera 2种草坪草对富营养化水体的净化能力.试验结果表明,2种草坪草对富营养化水体中的氮(N)、磷(P)、化学需氧量(CODcr)等具有明显的净化效果,尤其对总磷(TP)的净化能力最强,能有效改善富营养化水体的水质;2种草坪草之间,匍匐翦股颖对TP的去除效果显著高于高羊茅对TP的去除效果.研究结果显示浮床种植草坪草技术可能是一条有效的修复富营养化水域的新途径.  相似文献   

Potential exposure and risk assessment of agricultural workers to the insecticide chlorantraniliprole in rice paddies     

Jiho Lee  Junghak Lee  Minwoo Jung  Yongho Shin  JiWoo Kim  Jeong-Han Kim 《Pest management science》2023,79(2):678-687

  相似文献   

Intra- and interobserver assessments of intestinal wall thickness and segmentations from transverse sections of feline abdominal ultrasound images     

Jasmine Chang  Ian R. Porter  Marnin A. Forman  Natalya Shcherban  Parminder S. Basran 《Veterinary radiology & ultrasound》2023,64(1):131-139

Measurements of intestinal wall thicknesses from ultrasound imaging (US) are routinely used to support diagnoses of intestinal disorders in cats, however published studies describing observer agreement are currently lacking. The aim of this retrospective, observer agreement study was to quantify inter- and intraobserver repeatability and agreement in the measurement of intestinal wall layer thicknesses and the segmentation of transverse sections of small intestines in US images of 20 cats. Intestinal wall layer thickness measurements of the mucosa, submucosa, muscularis, serosa layer, and total thickness of these layers were performed on five cats with small cell epitheliotropic lymphoma, five with inflammatory bowel disease, and 10 with other conditions. Thickness measurements and the segmentation encompassing the serosa layer were obtained from five observers four times non-sequentially. The average standard deviation in thickness measurements (95% confidence interval) in the mucosa, submucosa, muscularis, serosa, and total thickness were 0.35 (0.07–0.95), 0.24 (0.07-0.52), 0.22 (0.06-0.49), 0.20 (0.05-0.49), and 0.57 (0.11-1.60) mm, respectively. The average intraclass correlation coefficients, which estimates the degree of consistency in thickness measurements and segmentation areas for each observer, ranged from 0.355 to 0.870 and 0.850 to 0.993, respectively. The interclass correlation coefficient, which estimates the degree of consistency when measuring a thickness or segmentation area over all observers ranged from 0.115 to 0.753, and 0.811 to 0.902, respectively. The overall average Dice Coefficient, which estimates the extent of overlap of the segmentations for all observers was 0.957 (0.933 to 0.972). Our results suggest segmentations of small intestines have a higher interobserver agreement than measurements of intestinal wall thicknesses.  相似文献   

Atypical antibiotic-responsive Staphylococcus pseudintermedius-associated tumor-like meibomitis in the upper eyelid: A case report of two dogs     

Sol Kim  Yeseul Yang  Su An Kim  SeungJun Lee  Yongbaek Kim  Kangmoon Seo  Seonmi Kang 《Veterinary ophthalmology》2023,26(5):458-463

  相似文献   

Tumour necrosis factor stimulated gene 6 intrinsically regulates PD-L1 expressions in breast cancer cells,leading to modulation of tumour microenvironment     

Jeong-Hwa Lee  Ju-Hyun An  Hwa-Young Youn 《Veterinary and comparative oncology》2023,21(2):255-269

Recent studies have shown that tumour cells express tumour necrosis factor-inducible gene 6 (TSG-6) and its protein, which is known to play a key role in regulating excessive immune responses and proliferation and growth of mesenchymal stem cells (MSCs). It has not been confirmed whether the inhibition of TSG-6 for tumour cells can suppress tumour cell growth and regulate the activation of immune cells in the tumour microenvironment (TME). TSG-6-specific small interfering RNA was transfected into canine and human breast cancer cells (CIPp, CIPm and BT-20). TSG-6-down-regulated (siTSG-6) cells showed decreased cell proliferation, migration, and invasion abilities. Decreased mRNA expressions of NF-κB, STAT3 and Sox2, confirming that TSG-6 is an upper factor governing tumour growth and metastasis. Notably, siTSG-6 cells showed significantly decreased expression levels of CD44 and PD-L1. Direct and indirect co-culture of canine peripheral blood mononuclear cells (cPBMCs) and the siTSG-6 cells showed significant activation in M1 type macrophages and cytotoxic T cells. They also showed a tendency to decrease in the expression of CTLA-4 and increase in the expression of PD-1. In conclusion, this study suggests that the down-regulation of TSG-6 in breast cancer cells could not only suppress tumour growth and metastasis, and but also regulate TME. Since modulation of immune checkpoint proteins occurs in both tumour cells and immune cells, inhibiting TSG-6 and its protein within the TME could be novel therapeutic target for anticancer treatment.  相似文献   

植物对磷饥饿的反应研究进展   总被引：4，自引：0，他引：4  

常胜合  舒海燕  秦广雍  吴玉萍  赵海珍 《中国农学通报》2005,21(7):199-199

磷是构成生命的重要元素之一,也是土壤中有效性最低的一种营养元素。我国是世界上最大的小麦生产国。但是我国耕地中有59%的土壤缺磷。农作物的产量常受到缺磷的影响而受损。土壤缺磷并不是土壤中总磷量低,而是土壤中可供植物直接吸收利用的有效态磷含量低。植物在磷饥饿时会发生各种各样的变化,以尽最大可能满足自身对磷的需求。植物对缺磷的反应是一个复杂的网络过程。大约有100多个基因参与了植物对缺磷的反应。其中主要的有磷转运蛋白基因、核糖核酸酶基因、磷酸酶基因等。植物在吸收外界的磷的过程中磷转运蛋白发挥了重要作用。植物磷转运蛋白基因按照序列相似性可以划分为H+/Pi共转运家族(Pht1家族)和Na+/Pi共转运家族(Pht2家族)。按照吸收动力学的标准可以分为高亲和力磷转运蛋白和低亲和力磷转运蛋白两种。磷饥饿时植物对磷吸收能力的增强的原因之一是增加了磷转运蛋白分子的合成数目。目前尽管人们对植物吸收磷的理解已经有了长足的进步,但是在植物对磷的具体调控机制、磷的跨液泡膜运输等重要方面仍然没有明确的结果。  相似文献   

Fault Section Diagnosis Algorithm of Distribution Networks Based On Topology Identification     

LIN Jing dong  CAO Chang xiu  ZHANG Bang li  WU Jian lin 《保鲜与加工》2001,(5):51-54

An algorithm of fault section diagnosis based on topology identification for distribution networks is presented. By decomposing the topologic matrix which describing the distribution network into two parts,one part only contain the complex coupling factor ,and the other ignore the complex coupling factor. Using this method, the fault zones in distribution network can be identified and isolated efficiently, and the vertexes of the zones can also be identified automatically. This approach adapts to the changefully network structures.  相似文献   

Analysis of Radial Forces on the Seal Block in a Compound Gear Pump with 3 Idle Gears     

ZHAO Lian chun  XU Xian liang  SUN Chang jing 《保鲜与加工》2001,(6):45-47

By analyzing the pressure distribution between gears and sealing blocks in compound gear pump with three idlers,the resultant hydraulic forces acting on seal blocks are obtained.The conclusion is the unbalanced resultant hydraulic forces are relatively small in magnitude.In the pump,the inner sealing edges are long and so it s Sealing effect is good,but the outer sealing effect is relatively poor because of its short sealing edges.The little unbalanced force pushes the sealing block to te pumping chamber,therefore it is advantageous to meliorating the pump's sealing effect,thereby to improving it's volumetric efficiency as well.  相似文献   

A FINITE ELEMENT ALGORITHM FOR PARALLEL PROCESSING OF MULTI-FRONTS     

Hu Ning Chang Ruqing 《保鲜与加工》1991,(4):16-19

In this paper,a new algorithm for solving large-scale finite element system is presented, which utilizes the architecture of parallel processing. Firstly, the structure is partitioned into sub-regions. Then multi-fronts are used to assemble and eliminate concurrently in every sub-region. Thus the condensed interface stiffness matrices and load vectors are obtained. By serially assembling and solving global equation to get interface displacements and backing to every sub-region, the inter-nodal displacements and element stresses can be solved. The results of computation show that it can not only increase the speed of computation,but also save the memory space of computer effectively. This algorithm is an effective method for solving large-scale structural finite element systems.  相似文献