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排序方式: 共有135条查询结果,搜索用时 31 毫秒
51.
Ryoyo Ikebuchi Satoru Konnai Tomohiro Okagawa Kazumasa Yokoyama Chie Nakajima Yasuhiko Suzuki Shiro Murata Kazuhiko Ohashi 《Veterinary research》2013,44(1):59
Programmed death-1 (PD-1) is a known immunoinhibitory receptor that contributes to immune evasion of various tumor cells and pathogens causing chronic infection, such as bovine leukemia virus (BLV) infection. First, in this study, to establish a method for the expression and functional analysis of bovine PD-1, hybridomas producing monoclonal antibodies (mAb) specific for bovine PD-1 were established. Treatment with these anti-PD-1 mAb enhanced interferon-gamma (IFN-γ) production of bovine peripheral blood mononuclear cells (PBMC). Next, to examine whether PD-1 blockade by anti-PD-1 mAb could upregulate the immune reaction during chronic infection, the expression and functional analysis of PD-1 in PBMC isolated from BLV-infected cattle with or without lymphoma were performed using anti-PD-1 mAb. The frequencies of both PD-1+ CD4+ T cells in blood and lymph node and PD-1+ CD8+ T cells in lymph node were higher in BLV-infected cattle with lymphoma than those without lymphoma or control uninfected cattle. PD-1 blockade enhanced IFN-γ production and proliferation and reduced BLV-gp51 expression and B-cell activation in PBMC from BLV-infected cattle in response to BLV-gp51 peptide mixture. These data show that anti-bovine PD-1 mAb could provide a new therapy to control BLV infection via upregulation of immune response. 相似文献
52.
Tomohiro Osaki Kazuo Azuma Seiji Kurozumi Yoshimori Takamori Takeshi Tsuka Tomohiro Imagawa Yoshiharu Okamoto Saburo Minami 《Marine drugs》2012,10(8):1873-1882
D-Glucosamine hydrochloride (GlcN∙HCl) is an endogenous amino monosaccharide synthesized from glucose that is useful in the treatment of joint diseases in both humans and animals. The aim of this study was to examine amino acid metabolism in dogs after oral administration of GlcN∙HCl. Accelerated fumarate respiration and elevated plasma levels of lactic acid and alanine were observed after administration. These results suggest that oral administration of GlcN∙HCl induces anaerobic respiration and starvation in cells, and we hypothesize that these conditions promote cartilage regeneration. Further studies are required to evaluate the expression of transforming growth factor-beta (TGF-β). 相似文献
53.
Kazuo Azuma Toshitsugu Ishihara Hiroyuki Nakamoto Takao Amaha Tomohiro Osaki Takeshi Tsuka Tomohiro Imagawa Saburo Minami Osamu Takashima Shinsuke Ifuku Minoru Morimoto Hiroyuki Saimoto Hitoshi Kawamoto Yoshiharu Okamoto 《Marine drugs》2012,10(10):2337-2348
We evaluated the anti-tumor activities of the oral administration of fucoidan extracted from Cladosiphon okamuranus using a tumor (colon 26)-bearing mouse model. The materials used included low-molecular-weight fucoidan (LMWF: 6.5–40 kDa), intermediate-molecular-weight fucoidan (IMWF: 110–138 kDa) and high-molecular-weight fucoidan (HMWF: 300–330 kDa). The IMWF group showed significantly suppressed tumor growth. The LMWF and HMWF groups showed significantly increased survival times compared with that observed in the control group (mice fed a fucoidan-free diet). The median survival times in the control, LMWF, IMWF and HMWF groups were 23, 46, 40 and 43 days, respectively. It was also found that oral administration of fucoidan increased the population of natural killer cells in the spleen. Furthermore, from the results of the experiment using Myd-88 knockout mice, it was found that these effects are related to gut immunity. These results suggest that fucoidan is a candidate anti-tumor functional food. 相似文献
54.
Junichiro Takeuchi Chie Imagawa Toshihiko Kawachi Koichi Unami Shigeya Maeda Tomoki Izumi 《Paddy and Water Environment》2010,8(2):175-187
A distributed hydro-environmental model is developed that achieves detailed analysis of the movement of water at a field-plot-scale
resolution in a mesoscale watershed including lowland areas where, especially for agricultures, it is an essential need to
get rid of redundant groundwater by drainage facilities such as rivers, canals and/or underdrains. For this, the problem geometry
is meshed with unstructured cells of triangular shape. Profile of a column cell is zoned into two: surface zone and groundwater
zone in which water movement is represented by combined tank and soil moisture sub-models, and well-defined two-dimensional
unconfined shallow groundwater flow sub-model, respectively. The top-two sub-models serve to evaluate evapotranspiration,
infiltration, soil water content, lateral surface water flow, and vertical percolation. The vertical percolation so evaluated
is given as longitudinal recharge to the bottom sub-model for computing groundwater flow. Surface water–groundwater interactions
through beds and stream-banks of perennial and ephemeral canals are considered by treating the canal courses as internal boundaries
in the groundwater flow model. The finite volume method (FVM) that allows of unstructured mesh and produces conservative solutions
is employed for groundwater flow computation. The model developed is applied to an actual watershed which includes a low-lying
paddy area to quantify the hydrological impact of land-use management practices over a period of 29 years in which the farmland
consolidation project was implemented and part of the paddy fields were converted to upland crop fields and housing lands.
From the results obtained, it is concluded that the model presently developed lends itself to water—as well as land-use management
practices. 相似文献
55.
Chie Imagawa Junichiro Takeuchi Toshihiko Kawachi Kei Ishida Shunsuke Chono Natsuki Buma 《Paddy and Water Environment》2011,9(4):425-439
Long-term simulation using the distributed hydro-environmental watershed model is efficacious for assessing irrigation impacts
on hydrological cycle in detail and for implementing watershed management successfully. In this article, the previously developed
hydro-environmental watershed model (HEWM-1) is improved in the water exchange process caused by surface water-groundwater
interaction via drainage canals and/or underdrains. The time-varying stream flow in canals is described by the complete one-dimensional
shallow water equations in a newly introduced submodel, the open channel flow submodel. This submodel coordinates with the
other submodels: the tank, soil moisture and groundwater flow submodels which are interlinked in a cascade manner. The improved
model (HEWM-2) is applied to an agricultural watershed covering an area from an alluvial fan onto a nearly level alluvial
plain, to be validated. The simulation by HEWM-2 is informative for identifying whether any drainage canal is gaining or losing
water in relation to groundwater level. It could thus provide useful information for conserving a complex network of drainage
canals which also functions as a passage for aquatic animals like fishes. 相似文献
56.
Tomohiro Osaki Seiji Kurozumi Kimihiko Sato Taro Terashi Kazuo Azuma Yusuke Murahata Takeshi Tsuka Norihiko Ito Tomohiro Imagawa Saburo Minami Yoshiharu Okamoto 《Marine drugs》2015,13(8):5007-5015
N-acetyl-d-glucosamine (GlcNAc) is a monosaccharide that polymerizes linearly through (1,4)-β-linkages. GlcNAc is the monomeric unit of the polymer chitin. GlcNAc is a basic component of hyaluronic acid and keratin sulfate found on the cell surface. The aim of this study was to examine amino acid metabolism after oral GlcNAc administration in dogs. Results showed that plasma levels of ectoine were significantly higher after oral administration of GlcNAc than prior to administration (p < 0.001). To our knowledge, there have been no reports of increased ectoine concentrations in the plasma. The mechanism by which GlcNAc administration leads to increased ectoine plasma concentration remains unclear; future studies are required to clarify this mechanism. 相似文献
57.
Ikuko Ito Toshikazu Yoneda Yoshihiko Omura Tomohiro Osaki Shinsuke Ifuku Hiroyuki Saimoto Kazuo Azuma Tomohiro Imagawa Takeshi Tsuka Yusuke Murahata Norihiko Ito Yoshiharu Okamoto Saburo Minami 《Marine drugs》2015,13(12):7463-7475
Urocanic acid is a major ultraviolet (UV)-absorbing chromophore. Chitins are highly crystalline structures that are found predominantly in crustacean shells. Alpha-chitin consists of microfibers that contain nanofibrils embedded in a protein matrix. Acid hydrolysis is a common method used to prepare chitin nanofibrils (NFs). We typically obtain NFs by hydrolyzing chitin with acetic acid. However, in the present study, we used urocanic acid to prepare urocanic acid chitin NFs (UNFs) and examined its protective effect against UVB radiation. Hos: HR-1 mice coated with UNFs were UVB irradiated (302 nm, 150 mJ/cm2), and these mice showed markedly lower UVB radiation-induced cutaneous erythema than the control. Additionally, sunburn cells were rarely detected in the epidermis of UNFs-coated mice after UVB irradiation. Although the difference was not as significant as UNFs, the number of sunburn cells in mice treated with acetic acid chitin nanofibrils (ANFs) tended to be lower than in control mice. These results demonstrate that ANFs have a protective effect against UVB and suggest that the anti-inflammatory and antioxidant effects of NFs influence the protective effect of ANFs against UVB radiation. The combination of NFs with other substances that possess UV-protective effects, such as urocanic acid, may provide an enhanced protective effect against UVB radiation. 相似文献
58.
The contribution of different proton sources to pedogenetic soil acidification was evaluated for three Japanese forest soils, i.e. ando soil, podzolic soil and brown forest soil in relation to the respective soil forming processes. Soil acidification rate and net proton generation were quantified based on the theory of proton budget for the respective soil horizon compartments (mainly the O, A and B horizons) by measuring fluxes of solutes entering and leaving the soil horizon compartment and vegetation uptake. Protons were produced by the dissociation of organic acids and nitrification in the O horizon and then consumed by adsorption and decomposition of organic acids and nitrate uptake by vegetation in deeper soil horizons at all plots. Excess uptake of cation over anion by vegetation was highest among proton sources in the whole soil compartment at all plots. Pedogenetic soil acidification was considered to include cation leaching from surface soil horizons due to proton generation by the dissociation of organic acids and nitrification and subsequent cation excess accumulation in wood in the growth stage of forests. In ando soil, andosolization resulted from the low contribution of net proton generation by the dissociation of organic acids as well as a lower soil acidification rate and complete acid neutralization. Dissolved organic carbon (DOC) fluxes in ando soil were lower than those in podzolic soil and brown forest soil due to high adsorption capacity of amorphous materials. In podzolic soil, podzolization resulted from intensive acidification in the O horizon, which derived from net proton generation by the dissociation of organic acids and nitrification as well as cation excess uptake by vegetation due to concentrated fine root biomass in the O horizon, and subsequent high proton efflux to subsoil. The high fluxes of DOC and Al leached from surface soil horizons were considered to contribute to eluviation of Al from surface soil and illuviation in subsoil in podzolic soil. In brown forest soil, brunification resulted from a lower DOC flux from the O horizon due to high decomposition and adsorption by oxides, where podzolization was weakened by high acid neutralization. Thus, the three representative processes involved in the pedogenesis of Japanese forest soils were well characterized by quantification of the respective proton-generating and consuming processes in each soil horizon. 相似文献
59.
60.
Bing‐nan Lin Yu‐chia Hsu Su‐chen Kuo Yann‐rong Lin Yong‐pei Wu Chie‐wei Kuo 《Plant Breeding》2017,136(2):206-213
From mutant pools of two Taiwanese elite japonica cultivars, Tainung 67 and Taikeng 8, we identified 13 mutant lines possessing opaque endosperm with relatively low amylose contents (AC) ranging from 1.5% to 7.1%. Because of different AC, paste viscosities of these 13 mutant lines differed, as revealed by palatability and physicochemical properties. The mutated gene conferring opaque endosperm was isolated from the F2 population of one mutant line, WY1× indica cv. ‘Taichung Sen 17’, by positional cloning, revealing a G3018→A3018 substitution at exon 9 of Waxy leading to a non‐synonymous mutation from alanine to valine. Two additional alleles were identified from the other 12 mutant lines, for which single‐nucleotide substitutions G2708 → A2708 and G3029 → A3029 occurred in exons 8 and 9, leading to non‐synonymous mutations from arginine to histidine and glutamic acid to lysine, respectively. The three novel wx alleles had different effects on grain quality, specifically on eating and cooking quality, and could be applied in rice breeding programmes to develop new low AC varieties by marker‐assisted selection. 相似文献