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Sophie Blani�� J��r��my Mortier Maxence Delverdier St��phane Bertagnoli Christelle Camus-Bouclainville 《Veterinary research》2009,40(1)
Myxoma virus (MYXV), a member of the Poxviridae family, is the agent responsible for myxomatosis, a fatal disease in the European rabbit (Oryctolagus cuniculus). MYXV has a linear double-stranded DNA genome that encodes several factors important for evasion from the host immune system. Among them, four ankyrin (ANK) repeat proteins were identified: M148R, M149R, M150R and M-T5. To date, only M150R and M-T5 were studied and characterized as critical virulence factors. This article presents the first characterization of M148R and M149R. Green Fluorescent Protein (GFP) fusions allowed us to localize them in a viral context. Whereas M149R is only cytoplasmic, interestingly, M148R is in part located in the nucleolus, a unique feature for an ANK repeat poxviral protein. In order to evaluate their implication in viral pathogenicity, targeted M148R, M149R, or both deletions were constructed in the wild type T1 strain of myxoma virus. In vitro infection of rabbit and primate cultured cells as well as primary rabbit cells allowed us to conclude that M148R and M149R are not likely to be implicated in cell tropism or host range functions. However, in vivo experiments revealed that they are virulence factors since after infection of European rabbits with mutant viruses, a delay in the onset of clinical signs, an increase of survival time and a dramatic decrease in mortality rate were observed. Moreover, histological analysis suggests that M148R plays a role in the subversion of host inflammatory response by MYXV. 相似文献
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Marc Babut Christelle Lopes Sébastien Pradelle Henri Persat Pierre-Marie Badot 《Journal of Soils and Sediments》2012,12(2):241-251
Purpose
Since 2005, freshwater fish contamination by polychlorobiphenyls (PCBs), polychlorodibenzodioxins, and polychlorodibenzofurans has been assessed in the Rhone River basin (France). A large database of surface sediment contamination by PCBs is also available, opening the way to the study of biota-to-sediment accumulation factor (BSAF) distribution throughout this basin. The ultimate goal of the study was to determine a sediment quality guideline (SQG) corresponding to the regulatory fish consumption limit. 相似文献23.
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Jessica K. Baron DVM DACVS-SA Sue A. Casale DVM DACVS Eric Monnet DVM PhD FAHA DACVS DECVS Philipp D. Mayhew BVM&S DACVS Jeffrey J. Runge DVM DACVS Christelle M. Follette DVM Kevin Phipps DVM Margaret E. Powell DVM Alicja I. Reczynska DVM Nathan T. Squire VMD Bruce A. Barton PhD John Berg DVM DACVS 《Veterinary surgery : VS》2020,49(Z1):O148-O155
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Antoine Decamp Orane Michelo Christelle Rabbat Cline Laroche Dominique Grizeau Jrmy Pruvost Olivier Gonalves 《Marine drugs》2021,19(2)
In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures. The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides’ (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system. Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization. Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis. Initial results showed that the enzymes were not sensitive to the presence of TFA and NaOH, so the methodology could be carried out on fresh hydrolysate. The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance. A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol. Finally, analyses of raw culture media were carried out and compared to the results obtained in miliQ water; no differences were observed. The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors. 相似文献
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Floridoside Extracted from the Red Alga Mastocarpus stellatus Is a Potent Activator of the Classical Complement Pathway 
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下载免费PDF全文 Anthony Courtois Christelle Simon-Colin Claire Boisset Christian Berthou Eric Deslandes Jean Guézennec Anne Bordron 《Marine drugs》2008,6(3):407-417
Many biological properties of algae have been found to have useful applications in human health, particularly in the fields of oncology and immunology. Floridoside, extracted from the red alga Mastocarpus stellatus, has a structure similar to the xenoantigen Gal alpha 1–3 Gal. This xenoantigen has been described to induce a high immune response in human xenografts and is mediated by natural anti-gal antibodies that activate the classical complement pathway. Based on this property, we analyzed the potential activities of floridoside on the immune system. We demonstrated that floridoside activates a complement cascade via the classical complement pathway, through the recruitment and activation of natural IgM. This algal molecule could represent an important step in the development of a potent new anticomplementary agent for use in therapeutic complement depletion. 相似文献
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Christelle Kouakou Jean-Pascal Bergé Régis Baron Laurent Lethuaut Carole Prost 《Journal Of Aquatic Food Product Technology》2013,22(5):453-467
The aim of this work was to study the effect of adding sugar during proteolysis to promote the Maillard reaction and mask the initial fish odor and off-flavors generated. An experimental design, based on the Doehlert plan, was used to study the influence of hydrolysis conditions (time, temperature, sugar, and antioxidant addition) on the odor characteristics of hydrolysates, soluble protein levels, and amino acid content. Results showed that the lowest level of sugar (10 g of D-xylose added to 1 kg of by-products) was enough to develop a grilled odor in hydrolysates. In the hydrolysis conditions used—i.e., enzyme inactivation at 95°C for 30 min—hydrolysis temperature had no effect on grilled odor production but significantly affected the soluble protein fraction, as did hydrolysis time. Soluble protein content and essential amino acid content increased with the enzymatic reaction but were not modified by adding sugar. Hydrolysis conditions that promote Maillard reactions while keeping a nutritional balance have been identified. 相似文献
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A pilot study of total and allergen‐specific IgE serum levels during anestrous,estrous and pregnancy in healthy female dogs 下载免费PDF全文
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