Detection of weed algae in open pond cultures of <Emphasis Type="Italic">Cyanobacterium aponinum</Emphasis> using PAM     

Dominik Winckelmann  Franziska Bleeke  Peter Bergmann  Clemens Elle  Gerd Klöck 《International Aquatic Research》2016,8(1):81-90

The potential use of non-arable land in the al-Wusta region of the Sultanate of Oman for the production of algae biomass was examined. Brackish cleaned production water from oil production supplemented with commercial fertilizer was used as growth medium. The indigenous isolate Cyanobacterium aponinum WP7(1) was grown in open ponds using batch or semi-continuous cultivation. Biomass production rates of 15–24 g/m²/day were achieved. The change of salinity due to evaporation, which was thought to be a major challenge, did not exceed 35 ppt. All cultures showed contaminations with weed algae. Contaminations with green algae or diatoms were detectable using fluorescence pattern excited by four different wavelengths using a pulse-amplitude-modulation chlorophyll fluorometer (PAM). It is possible to estimate the health level and the mayor groups of which a culture is composed using the PAM method. Therefore, the fluorescence of the photosynthetically inactive sample is compared with the fluorescence after all copies of photosystem II were closed by exposing the sample to a high-intensity light beam. A detection limit of one weed algae cell in a hundred cells was achieved.  相似文献   

Anesthesia case of the month. Development of the Cushing reflex secondary to a dangerous increase in intracranial pressure     

Abelson AL  Faissler D  Karas AZ 《Journal of the American Veterinary Medical Association》2008,232(9):1298-1300

  相似文献   

In search of the source of dermatophytosis: Epidemiological analysis of Trichophyton verrucosum infection in llamas and the breeder (case report)     

Dominik &#x;agowski  Sebastian Gnat  Aneta Nowakiewicz  Marcelina Osi&#x;ska  Aleksandra Trocia&#x;czyk  Przemys&#x;aw Zi&#x;ba 《Zoonoses and public health》2019,66(8):982-989

During the last few years, the number of cases of Trichophyton verrucosum isolation from humans suffering from mycoses has been constantly increasing, which is correlated with the presence of an increasing number of outdoor breeding farms. Farmers and their families as well as veterinarians and technicians involved in handling the animals are at a higher risk of infection. One of the most important aims of mycological diagnostics is epidemiological analysis. Typically, the history of the disease is not sufficient to indicate reliably and eliminate the outbreak of infection. PCR fingerprinting methods are a useful tool in this type of analysis, which is presented in this study. The main aim is to present diagnostic and epidemiological analyses of dermatophyte isolates from llamas and their breeder. In two llamas, round alopecia sites or ca. 2‐cm excoriations covered with thickened scaling epidermis were noticed at the border of the head and neck with a distinct tendency towards hair loss. Tinea unguium was noticed in a nail of the breeder's right hand. Direct analysis of the material from the clinical lesions revealed the presence of arthrospores. The macro‐ and micromorphology of the isolates were homogeneous and characteristic for T. verrucosum. The identification analysis based on the ITS sequences confirmed the previous morphological diagnostic examination. The MP‐PCR and MSP‐PCR analysis indicated high invariability of the genomes of the strains isolated from the human and animals. The epidemiological research has indicated an identical source of dermatophyte infection in the breeder and the lamas. To sum up, the number of pets and farm animals is increasing and dermatologists should always be informed about possible dermatophyte transmission sources. The possibility of transmission of zoophilic dermatophytes from humans to animals is a suggestion for further analysis; therefore, this type of transmission should be considered in dermatological studies.  相似文献   

Analyzing land use change to identify migration corridors of African elephants (<Emphasis Type="Italic">Loxodonta africana</Emphasis>) in the Kenyan-Tanzanian borderlands     

Dominik Schüßler  Phyllis C. Lee  Robin Stadtmann 《Landscape Ecology》2018,33(12):2121-2136

  相似文献   

Prolonged survival after craniectomy with skull reconstruction and adjuvant definitive radiation therapy in three dogs with multilobular osteochondrosarcoma     

Molly E. Holmes  Michele A. Keyerleber  Dominik Faissler 《Veterinary radiology & ultrasound》2019,60(4):447-455

Multilobular osteochondrosarcoma is an uncommon canine tumor but presents a treatment challenge when arising on the skull. This retrospective case series study aimed to describe outcome of a multimodality treatment approach involving aggressive surgical resection and adjuvant definitive radiation therapy in a group of dogs with multilobular osteochondrosarcoma of the calvarium. Clinical, imaging, treatment, and outcome data were collected from retrospective review of medical records. Three dogs met inclusion criteria. The presenting clinical complaint was the presence of a mass effect of the skull in all three dogs and concurrent neurologic abnormalities in one dog. Advanced imaging revealed aggressive lytic and proliferative tumors arising from the calvarium in all three dogs. All dogs were treated surgically with a modified craniectomy, repaired with a titanium mesh—polymethyl methacrylate bone cement implant or a low prolife titanium mesh plate and followed by adjuvant definitive radiation therapy with 2.5 Gy per fraction for 22 daily fractions. There were no major immediate surgical complications and radiation was well tolerated overall. Neurologic improvement was seen in the patient that presented with neurologic disease. Survival times from surgery were 387, 422, and 730 days and from the time of radiation were 358, 397, and 677 days. Findings in this sample of three dogs supported the use of aggressive therapy with a combination of surgical craniectomy and cranioplasty utilizing a titanium mesh implant and high dose definitive radiation therapy for local control and prolonged survival times in dogs with multilobular osteochondrosarcoma of the skull.  相似文献   

Sources of soil CO2 in calcareous grassland with woody plant encroachment     

Gregor Plestenjak  Klemen Eler  Dominik Vodnik  Mitja Ferlan  Matja? ?ater  Tja?a Kandu?  Primo? Simon?i?  Nives Ogrinc 《Journal of Soils and Sediments》2012,12(9):1327-1338

Purpose

The objective of this study is to estimate the contribution of various sources that influence soil CO₂ concentrations in calcareous grassland.

Materials and methods

The research was performed at the Podgorski Kras plain (45?°33?? N, 13?°55?? E, 400?C430?m.a.s.l.) in the sub-Mediterranean region of Slovenia (SW Slovenia), where many meadows and pastures have been abandoned. In parallel to the measurement of soil respiration R _s, soil gas was sampled for stable isotope analysis. Samples were taken biweekly at two sites, Grassland and Invaded, from July 2008 until November 2010. In addition, daily variations in concentration and stable isotope composition of soil CO₂ were determined in May 2009. The partitioning of soil CO₂ concentrations was performed using stable isotope mass balance calculation.

Results and discussion

The concentration and isotope composition of soil CO₂ exhibited similar seasonal variations at both sites. Lower ??¹³C_CO2 values, ranging from ?28.2 to ?15.2 ??, which occurred during warm periods and higher values, up to ?12.1 ??, were typical of cold winter periods, from December to March. Organic sources were estimated to constitute between 78 and 99?% of total soil CO₂ during warmer periods from May until October. This contribution was lower during the winter, ranging from 46 to 77?%. In winter, the atmospheric component to soil CO₂ dominated, constituting up to 60?%. On average, the inorganic contribution was estimated to comprise 12?% of the soil CO₂ at all sampling locations. The contribution of this source to soil CO₂ concentration, at up to 41?%, was highest in Grassland during the growing season. The inorganic source of soil CO₂ was also an important component during daily variations. The highest contribution was observed during the day, in parallel to the highest respiration rates.

Conclusions

The inorganic pool is shown to be an important part of soil CO₂ in calcareous areas and should be considered as equal to organic CO₂ as a source in soil CO₂ partitioning.  相似文献   

Compartment-specific antioxidative defense in Arabidopsis against virulent and avirulent Pseudomonas syringae     

Großkinsky DK  Koffler BE  Roitsch T  Maier R  Zechmann B 《Phytopathology》2012,102(7):662-673

The accumulation of reactive oxygen species (ROS) during biotic stress is either part of a hypersensitive response of the plant or induced directly by the pathogen. Antioxidants such as ascorbate and glutathione counteract the accumulation of ROS and are part of the defense reaction. The aim of the present study was to investigate the compartment-specific importance of ascorbate and glutathione during a virulent and avirulent Pseudomonas syringae infection in Arabidopsis thaliana. Peroxisomes were found to be the hotspot for glutathione accumulation reaching 452% and 258% of control levels 24 h postinoculation during the virulent and avirulent infection, respectively. An accumulation of ascorbate could also be observed in vacuoles during Pseudomonas syringae infection, whereas glutathione remained absent in this cell compartment. Neither glutathione nor ascorbate accumulated in the apoplast during pathogen infection demonstrating an only negligible role of these antioxidants in the apoplast during pathogen infection. Compartment-specific changes followed a recently proposed stress model with an increase of ascorbate and glutathione in most cell compartments at the early stages of infection and a strong drop at the later stage of infection when a strong accumulation of ROS and symptoms occurred in the leaves. This study highlights the importance of certain cell compartments and antioxidants in general for the protection of pathogen-induced ROS accumulation.  相似文献   

Habitat use of large ungulates in northeastern Germany in relation to forest management     

Eric Heinze  Steffen BochMarkus Fischer  Dominik HessenmöllerBernd Klenk  Jörg MüllerDaniel Prati  Ernst-Detlef SchulzeCarolin Seele  Stephanie SocherStefan Halle 《Forest Ecology and Management》2011,261(2):288-296

Estimating large herbivore density has been a major area of research in recent decades. Previous studies monitoring ungulate density, however, focused mostly on determining animal abundance, and did not interpret animal distribution in relation to habitat parameters. We surveyed large ungulates in the Biodiversity Exploratory Schorfheide-Chorin using faecal pellet group counts. This allowed us to explore the link between relative ungulate abundance, habitat use, and browsing damage on trees in a region with several types of forest, including unharvested and age-class beech forests, as well as age-class pine forests. Our results demonstrate that roe deer and fallow deer relative abundance is negatively correlated with large tree cover, and positively correlated with the cover of small shrubs (Rubus spec., Vaccinium spec.), and winter food supply. Habitat use of roe deer and fallow deer, as estimated by counting faecal pellet groups, revealed a preference for mature pine forests, and avoidance of deciduous forests. This differential habitat use is explained by different distributions of high quality food resources during winter. The response of deer to understory cover differed between roe deer and fallow deer at high cover percentages. The amount of browsing damage we observed on coniferous trees was not consistent with the relative deer abundance. Browsing damage was consistently higher on most deciduous trees, except for beech saplings which sustained less damage when roe deer density was low. Because roe deer is a highly selective feeder, it was reported to affect tree diversity by feeding only on trees with high nutritional value. Consequently, we propose that managing the number of all deer species by hunting is necessary to allow successful forest regeneration. Such an adjustment to deer numbers would need to account for both current tree diversity and alternative food resources. Our findings may be applicable to other forest landscapes in northeastern Germany including mature pine stands and differently harvested deciduous forests.  相似文献   

Associations between immune competence phenotype and feedlot health and productivity in Angus cattle     

Brad C Hine  Amy M Bell  Dominic D O Niemeyer  Christian J Duff  Nick M Butcher  Sonja Dominik  Laercio R Porto-Neto  Yutao Li  Antonio Reverter  Aaron B Ingham  Ian G Colditz 《Journal of animal science》2021,99(2)

Genetic strategies aimed at improving general immune competence (IC) have the potential to reduce the incidence and severity of disease in beef production systems, with resulting benefits of improved animal health and welfare and reduced reliance on antibiotics to prevent and treat disease. Implementation of such strategies first requires that methodologies be developed to phenotype animals for IC and demonstration that these phenotypes are associated with health outcomes. We have developed a methodology to identify IC phenotypes in beef steers during the yard weaning period, which is both practical to apply on-farm and does not restrict the future sale of tested animals. In the current study, a total of 838 Angus steers, previously IC phenotyped at weaning, were categorized as low (n = 98), average (n = 653), or high (n = 88) for the IC phenotype. Detailed health and productivity data were collected on all steers during feedlot finishing, and associations between IC phenotype, health outcomes, and productivity were investigated. A favorable association between IC phenotype and number of mortalities during feedlot finishing was observed with higher mortalities recorded in low IC steers (6.1%) as compared with average (1.2%, P < 0.001) or high (0%, P = 0.018) IC steers. Disease incidence was numerically highest in low IC steers (15.3 cases/100 animals) and similar in average IC steers (10.1 cases/100 animals) and high IC steers (10.2 cases/100 animals); however, differences between groups were not significant. No significant influence of IC phenotype on average daily gain was observed, suggesting that selection for improved IC is unlikely to incur a significant penalty to production. The potential economic benefits of selecting for IC in the feedlot production environment were calculated. Health-associated costs were calculated as the sum of lost production costs, lost capital investment costs, and disease treatment costs. Based on these calculations, health-associated costs were estimated at AUS$103/head in low IC steers, AUS$25/head in average IC steers, and AUS$4/head in high IC steers, respectively. These findings suggest that selection for IC has the potential to reduce mortalities during feedlot finishing and, as a consequence, improve the health and welfare of cattle in the feedlot production environment and reduce health-associated costs incurred by feedlot operators.  相似文献   

Active immunisation against GnRH as treatment for unilateral granulosa theca cell tumour in mares     

Daphne Behrendt  Dominik Burger  Steffen Gremmes  Katja Szunyog  Selena Röthemeier  Harald Sieme 《Equine veterinary journal》2021,53(4):740-745

  相似文献