Modulation of matrix metalloproteinases by ultraviolet radiation in the canine cornea     

Chandler HL  Kusewitt DF  Colitz CM 《Veterinary ophthalmology》2008,11(3):135-144

Purpose To determine whether ultraviolet (UV) radiation can modulate expression and regulation of matrix metalloproteinases (MMP) in the canine cornea and to examine the expression of MMPs in canine chronic superficial keratitis (CSK). Methods Immunohistochemistry for MMP‐2 and MMP‐9 was performed on samples of CSK. In vitro, canine corneal epithelial cell (CEC) and stromal cell cultures were exposed to UV‐irradiation. Following 2, 8 or 24 h, cells were harvested. MMP expression was examined by zymography, and RT‐PCR was used to examine expression of Slug and Snail. CEC cultures treated with an EGFR inhibitor or a p38 inhibitor were UV‐exposed and harvested 24 h later to examine expression of MMPs, Slug and Snail. Results Canine CSK had increased immunopositivity for both MMP‐2 and MMP‐9 compared to normal canine corneas. In vitro, CEC and stromal cell cultures exposed to UV showed generally increased expression of MMP‐2, ‐9, Slug, and Snail; this response was dose and time dependent. Inhibition of the EGFR pathway did not prevent increased expression of MMP‐2, ‐9, Slug or Snail in UV‐exposed CEC; however, p38 inhibition did attenuate UV induction. Conclusions We have found increased expression of MMPs in clinical samples of CSK compared to normal corneas. In addition, we have shown that there is a temporal association and dose dependency between UV exposure and production of MMPs, Slug, and Snail. These findings suggest that overexpression of MMPs due to UV‐exposure may be linked to changes in the cornea that allow an influx of inflammatory cells and vascularization.  相似文献   

Use of hepatic protein biomarkers for tracing the exposure of veal calves to illegal growth-promoters: investigations on experimental samples and preliminary application under field conditions     

Nebbia C  Della Donna L  Carletti M  Balbo A  Barbarino G  Gardini G 《Journal of veterinary pharmacology and therapeutics》2008,31(3):272-275

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Principles of Laparoscopic Hemostasis: Ligation Techniques     

Donna L. Shettko  Dean A. Hendrickson 《Journal of Equine Veterinary Science》2008

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Infiltration-RNAseq: transcriptome profiling of Agrobacterium-mediated infiltration of transcription factors to discover gene function and expression networks in plants     

Donna?M.?BondEmail author  Nick?W.?Albert  Robyn?H.?Lee  Gareth?B.?Gillard  Chris?M.?Brown  Roger?P.?Hellens  Richard?C.?MacknightEmail author 《Plant methods》2016,12(1):41

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Stolon Development and Cultural Production Practices of Winter-Grown Strawberries     

Donna M. Morrison  Erin E. Blankenship  Paul E. Read  Ellen T. Paparozzi 《International Journal of Fruit Science》2018,18(2):138-152

ABSTRACT

The four experiments described here are part of a 5-year program focused on determining whether day-neutral and short-day strawberries would be suitable for commercial off-season/winter (temperate climate) production in greenhouses. All research was performed using container-grown plants fertigated through a capillary mat production system with heat supplied under the benches. The first experiment focused on stolon development. The second experiment investigated the influence of stolon removal on berry production and included the removal of flowers for the first three weeks. The third and fourth experiments were conducted as a comparison of university research (experiment 3) versus a commercial operation (experiment 4). In experiment one, of 13 cultivars, ‘AC Wendy’ plants produced significantly more stolons than the other cultivars. In experiment two, stolon production peaked at weeks 12 and 13, with ‘Chandler’ plants producing significantly more stolons (starting at week 9) than the other cultivars. At week 13, ‘Seascape’ plants produced more total berry weight than the other cultivars. The influence of stolon removal on total berry weight varied, with ‘Evie-2+’ plants (large crowns) producing higher total berry weight with the stolons on as compared to ‘Evie-2’ plants, which produced higher total berry weight with the stolons removed. In experiment 2, fruit production was low and was probably a result of deflowering for three weeks. Consequently, it may not be advisable to deflower during the ‘off-season’ for greenhouse-grown strawberries. In the final two experiments, which involved a commercial cooperator as well as the university greenhouse, it was found that of the five cultivars trialed, the same cultivars at both sites produced the maximum total berry weight. Stolon production differed at the two locations, but the optimal time for stolon removal was similar. Thus, it is recommended that for winter greenhouse production, stolons be removed initially at week 8 and then at weeks 11–12 of the production cycle.  相似文献   

Managing invasive plants on public conservation forestlands: Application of a bio-economic model   总被引：1，自引：0，他引：1  

Donna J. Lee  Damian C. Adams  C.S. Kim   《Forest Policy and Economics》2009,11(4):237-243

Public conservation forestlands protect natural resources, provide wildlife habitat, and service a multi-billion dollar recreation industry. Invasive upland plants (IPs) threaten to dramatically reduce the flow of goods and services from these lands. In the late 1990s, a Florida (USA) state program began combating IPs on public conservation lands. By 2007, the program had controlled roughly 1/3 of the 1.5 million IP-infested acres, primarily due to a surge in expenditures that began in 2001 and peaked in 2005. This study evaluates the effectiveness and efficiency of the program by simulating the costs and benefits of IP control through 2016 under five feasible policy alternatives: (I) Do nothing, (II) Maintenance control, (III) 2001–2004 level (of program spending), (IV) 2005 level, and (V) Maximum net present value. To evaluate the policies, we construct a bio-economic model that is parameterized using observations of IP coverage and data from 11 state regions. Given the uncertain nature of IP spread, we simulate the policies' economic impacts under two plausible spread rates. Simulation results indicate that the program's recent efforts are highly effective – generating up to $865.1 million in present value net benefits through 2016 – but less efficient than more costly, front-loaded spending that can generate up to 4.37 times more net benefits.  相似文献   

Considering Food and Society by William Whit     

Donna Maurer  Mia Moore Barker  Jacqueline M. Newman  William C. Whit 《Agriculture and Human Values》1998,15(1):85-89

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Corrigendum     

Patrick C. Tobin  Barry B. Bai  Donald A. Eggen  Donna S. Leonard 《国际虫害防治杂志》2013,59(4)

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Authenticity of PGI "Clementine of Calabria" by multielement fingerprint     

Benabdelkamel H  Di Donna L  Mazzotti F  Naccarato A  Sindona G  Tagarelli A  Taverna D 《Journal of agricultural and food chemistry》2012,60(14):3717-3726

Clementine is a citrus fruit that has found a peculiar habitat in specific areas of Calabria, a region located in southern Italy. Due to its peculiar characteristics it was recently awarded with protected geographical indications (PGI) from the European Union. In this work, stepwise linear discriminant analysis (S-LDA), soft independent modeling of class analogy (SIMCA), and partial least-squares discriminant analysis (PLS-DA) were used to build chemometric models able to protect PGI Clementine from others of different origin. Accordingly, the concentration of 24-26 elements was determined in peel and juice samples, respectively, obtained from Calabrian PGI clementine and from fruits cultivated in Algeria, Tunisia, and Spain. A cross-validation procedure has shown very satisfactory values of prediction ability for both S-LDA (96.6% for juice samples and 100% for peel samples) and SIMCA (100% for both peel and juice samples). PLS-DA models also yielded satisfactory results.  相似文献   

Predictors of anaemia and iron deficiency in HIV-infected pregnant women in Tanzania: a potential role for vitamin D and parasitic infections     

Finkelstein JL  Mehta S  Duggan CP  Spiegelman D  Aboud S  Kupka R  Msamanga GI  Fawzi WW 《Public health nutrition》2012,15(5):928-937

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