Phenolic Acids Profiles and Cellular Antioxidant Activity in Tortillas Produced from Mexican Maize Landrace Processed by Nixtamalization and Lime Extrusion Cooking     

Nallely Gaxiola-Cuevas  Saraid Mora-Rochín  Edith Oliva Cuevas-Rodriguez  Liliana León-López  Cuauhtémoc Reyes-Moreno  Alvaro Montoya-Rodríguez  Jorge Milán-Carrillo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):314-320

Phenolic acids profiles, chemical antioxidant activities (ABTS and ORAC), as well as cellular antioxidant activity (CAA) of tortilla of Mexican native maize landraces elaborated from nixtamalization and lime cooking extrusion processes were studied. Both cooking procedures decreased total phenolics, chemicals antioxidant activity when compared to raw grains. Extruded tortillas retained 79.6–83.5%, 74.1–77.6% and 79.8–80.5% of total phenolics, ABTS and ORAC values, respectively, compared to 47.8–49.8%, 41.3–42.3% and 43.7–44.4% assayed in traditional tortillas, respectively. Approximately 72.5–88.2% of ferulic acid in raw grains and their tortillas were in the bound form. Regarding of the CAA initially found in raw grains, the retained percentage for traditional and extruded tortillas ranged from 47.4 to 48.7% and 72.8 to 77.5%, respectively. These results suggest that Mexican maize landrace used in this study could be considered for the elaboration of nixtamalized and extruded food products with nutraceutical potential.  相似文献   

Kinetic Parameters of Changes in Sensory Characteristics of Minimally Processed Rambutan     

Gladys González González  Rosa Ma. Salinas Hernández  Andrea Marcela Piagentini  Fidel Ulín Montejo  Edith Miranda Cruz  María Élida Pirovani 《International Journal of Fruit Science》2016,16(2):159-170

The aim of this work was to develop kinetic models to describe the sensory changes of minimally processed rambutan. Quality was assessed by a trained panel. The attributes that most decreased were freshness, aroma, and appearance (17–38%), and the defects that were more increased were off-odors and off-flavors (28–67%). Although whole fruit is sensitive to chilling injury, minimally processed rambutan is preserved better at 4.6 °C. Color, sweetness, and off-odor changes were adjusted to first-order kinetic and the other characteristics to zero order. Sourness, aroma, and color have higher activation energies (235–297.4 KJ mol^–1), and these characteristics would change faster if failure in the cold chain occurs.  相似文献   

Histological and anatomical variations of septomarginal trabecula in bovine hearts     

Srgio Ricardo Rios Nascimento  Cristiane Regina Ruiz  Samara Urban de Oliva 《Anatomia, histologia, embryologia》2019,48(3):228-233

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Farmers’ adoption of maize (Zea mays L.) hybrids and the persistence of landraces in Southwest China: implications for policy and breeding   总被引：1，自引：1，他引：0  

Jingsong Li  Edith T. Lammerts van Bueren  Janice Jiggins  Cees Leeuwis 《Genetic Resources and Crop Evolution》2012,59(6):1147-1160

This paper examines changes in the distribution of maize hybrids and landraces in the mountainous areas of southwest China over 1998–2008, farmers’ reasons for cultivar adoption and the implications for national policies in relation to seed production and breeding, based on baseline data and a survey conducted in Guangxi, Yunnan and Guizhou. The study traced the dynamic changes in the adoption of hybrids and landraces in farmers’ fields, explored how individual farmer’s choices can influence local landrace distribution, and investigated the space for conducive policy and innovative action for on-farm conservation of maize genetic resources. The research showed that although there is strong farmers’ interest in accessing modern maize hybrids, farmers also express strong reasons for maintaining at least some of the landraces that satisfy local agronomic context and social preferences. Farmers recognized that hybrids have a number of advantages but they also indicated some disadvantages of the current available hybrids e.g. with respect to seed quality, local adaptability, taste and cost of seeds, but also lack of information on the performances of the new hybrids. Based on farmers’ reasoning and experiences, the requirements have been identified for improving yield combined with local preferences (agronomic, cultural and socio-economic). The paper concludes by identifying options for how China might seek to develop resilient seed systems for smallholder farmers in poor areas, under changing climatical conditions and volatile markets. Participatory Plant Breeding is among the options considered for bringing farmers’ needs into conservation and breeding strategies for improving local adaptation.  相似文献   

Tropical Rice Cultivars from Lowland and Upland Cropping Systems Differ in Iron Plaque Formation     

Eduardo Gusmão Pereira  Marco Antonio Oliva  Advânio Inacio Siqueira-Silva  Laíse Rosado-Souza  Daniel Teixeira Pinheiro  Andréa Miyasaka Almeida 《Journal of plant nutrition》2014,37(9):1373-1394

In iron toxic wetlands, ferric hydroxide is commonly deposited on rice roots. This study aims to to evaluate the differences in iron plaque formation in rice cultivars from different cropping systems. Thirty days old seedlings of Brazilian rice cultivars from the lowland cropping system (‘BRS Atalanta’ and ‘Epagri 107’) and upland cropping system (‘Canastra’) or both systems (‘BRSMG Curinga’) and the cultivar ‘Nipponbare’ were exposed to iron excess [4 mM iron sulfate heptahydrate (FeSO₄.7H₂O)] for seven days in nutrient solution. It was observed iron plaque formation and ruptures of the root epidermal cells. The lowland cultivars showed higher Fe content in iron plaque. Iron stain was detected in the root hairs, epidermis, hypodermis, and exodermis. The root exodermis may be contributed to prevent the deposit of iron in the cortex of the lowland cultivars and in the cultivar ‘BRSMG Curinga’. It was observed in plants with iron plaque formation significant reductions in the shoot content of phosphorous, manganese and magnesium due to different causes. The differences in iron plaque formation among the cultivars might be an indicative of variations in exodermis selectivity, root oxidative capacity, and iron nutrition mechanisms.  相似文献   

Modulation of the immunogenicity of the Trypanosoma congolense cysteine protease, congopain, through complexation with ��2-macroglobulin     

Laura Elizabeth Joan Huson  Edith Authié  Alain Fran?ois Boulangé  James Phillip Dean Goldring  Theresa Helen Taillefer Coetzer 《Veterinary research》2009,40(6)

The protozoan parasite Trypanosoma congolense is the main causative agent of livestock trypanosomosis. Congopain, the major lysosomal cysteine proteinase of T. congolense, contributes to disease pathogenesis, and antibody-mediated inhibition of this enzyme may contribute to mechanisms of trypanotolerance. The potential of different adjuvants to facilitate the production of antibodies that would inhibit congopain activity was evaluated in the present study. Rabbits were immunised with the recombinant catalytic domain of congopain (C2), either without adjuvant, with Freund’s adjuvant or complexed with bovine or rabbit α₂-macroglobulin (α₂M). The antibodies were assessed for inhibition of congopain activity. Rabbits immunised with C2 alone produced barely detectable anti-C2 antibody levels and these antibodies had no effect on recombinant C2 or native congopain activity. Rabbits immunised with C2 and Freund’s adjuvant produced the highest levels of anti-C2 antibodies. These antibodies either inhibited C2 and native congopain activity to a small degree, or enhanced their activity, depending on time of production after initial immunisation. Rabbits receiving C2-α₂M complexes produced moderate levels of anti-C2 antibodies and these antibodies consistently showed the best inhibition of C2 and native congopain activity of all the antibodies, with maximum inhibition of 65%. Results of this study suggest that antibodies inhibiting congopain activity could be raised in livestock with a congopain catalytic domain-α₂M complex. This approach improves the effectiveness of the antigen as an anti-disease vaccine candidate for African trypanosomosis.  相似文献   

Deltamethrin-impregnated collars for the control of canine leishmaniasis: evaluation of the protective effect and influence on the clinical outcome of Leishmania infection in kennelled stray dogs     

Foglia Manzillo V  Oliva G  Pagano A  Manna L  Maroli M  Gradoni L 《Veterinary parasitology》2006,142(1-2):142-145

A 2-year field study on kennelled stray dogs living in a highly endemic area of leishmaniasis was designed to evaluate whether deltamethrin-impregnated collars (Scalibor) Protector Band) could confer protection against leishmaniasis in this peculiar setting, and to assess differences in clinical outcomes between collared and uncollared dogs. A cohort of 120 clinically healthy and Leishmania-seronegative dogs was enrolled, 50% of which were collared before the 2003 transmission season, and then re-collared before the subsequent season. Collared and uncollared animals were allowed to live with infected dogs in same groups within the kennel. Follow-up included serological (IFAT) assessment twice a year with parasitological Leishmania confirmation, and clinical evaluation performed every 3 months on seroconverted dogs from both groups. Collar losses during the two seasons were high (35%). About 50% of enrolled dogs were lost at follow-up because of death or they were moved to other locations. After the 2003 season, cross-sectional serological examinations tested positive in 5 out of 44 collared animals (11.4%) and in 14 out of 34 controls (41.2%), with 72.3% estimated protection (P<0.005). After the 2004 season, 7/31 seronegative collared dogs seroconverted (22.6%) compared with 7/17 seronegative controls (41.2%), with 45.1% protection (P=0.15). At the end of the study, the cumulative rate of protection was 50.8% (P=0.005). At the clinical evaluation of 21 seroconverted dogs from both groups, canine leishmaniasis signs were significantly more frequent (90% versus 36%, P=0.017) and rapidly progressive in uncollared than in collared dogs. Reasons for such partial clinical protection in collared dogs may be found in the vector anti-feeding effect of protector bands, resulting in a lower number of infectious bites and, probably, in the reduction of antigenic stimuli necessary to shift toward a non-protective immune response.  相似文献   

A rebuttal to the letter to the editor concerning ‘Defining species boundaries in the genus Phytophthora: the case of Phytophthora andina’     

G. A. Forbes  J. B. Ristaino  R. F. Oliva  W. Flier 《Plant pathology》2012,61(2):221-223

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应用ＥＬＩＳＰＯＴ试验测定重组卡介苗诱导的Ｔ细胞应答   总被引：7，自引：1，他引：6  

焦新安  Richard Lo-Man  Edith D'eriaud  Nathalie Winter  Claude Leclerc  刘秀梵 《中国预防兽医学报》2001,23(1):46-49

应用免疫磁性分离技术去除免疫小鼠脾脏细胞中ＣＤ４^ 或ＣＤ8^ Ｔ细胞后，在酶免疫斑点试验中证实表达大肠杆菌ＭalE蛋白的重组卡介苗（rBCG.MalE)诱导的Ｔ细胞应答是ＣＤ４^ t细胞依赖的对MalE、PPD持异Ｔ细胞应答的动态分析结果表明，，rBCG.MalE、ＢＣＧ诱导的特异ＣＤ４^ T细胞应答存在Ｔh1/Th2平衡转换现象，即起始阶段为Ｔh1应答，一段时间后出现Ｔh2应答，并逐步形成Ｔh1/Th２混合应答。这些结果，分为枝杆菌Ｔ细胞应答规律提供了新的认识，同时，亦表明ＢＣＧ是优良的外源抗原表达与运送载体。  相似文献   

Hot water dipping of olives (Olea europaea) for virgin oil debittering     

García JM  Yousfi K  Oliva J  García-Diaz MT  Pérez-Camino MC 《Journal of agricultural and food chemistry》2005,53(21):8248-8252

Olives (Olea europaea L.) of the Manzanilla, Picual, and Verdial varieties harvested at the green mature stage of ripening were dipped in hot water at a range of temperatures between 60 and 72 degrees C for 3 min. Immediately after treatment, oils were physically extracted from the olives. Olive heating promotes a reduction of oil bitterness in direct relationship to the temperature used. Fruit heating at > or =60 degrees C for 3 min did not cause significant changes in acidity, UV absorption, peroxide index, and panel test score of the oils obtained but decreased its oxidative stability. Oils extracted from heated fruit showed higher concentrations of chlorophylls and carotenes and lower total phenol content.  相似文献