Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia     

Toplak I  Lazić S  Lupulović D  Prodanov-Radulović J  Becskei Z  Došen R  Petrović T 《Acta veterinaria Hungarica》2012,60(3):409-420

Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.  相似文献   

Human as a source of tuberculosis for cattle. First evidence of transmission in Poland     

Krajewska M  Kozińska M  Zwolska Z  Lipiec M  Augustynowicz-Kopeć E  Szulowski K 《Veterinary microbiology》2012,159(1-2):269-271

  相似文献   

Advanced electrocardiographic parameters change with severity of mitral regurgitation in Cavalier King Charles Spaniels in sinus rhythm     

Spiljak Pakkanen M  Domanjko Petrič A  Olsen LH  Stepančič A  Schlegel TT  Falk T  Rasmussen CE  Starc V 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2012,26(1):93-100

  相似文献   

Changes in the content of free sulphydryl groups during postharvest wheat and flour maturation and their influence on technological quality     

Jelena Tomić  Milica PojićAleksandra Torbica  Slađana RakitaDragan Živančev  Elizabet Janić HajnalTamara Dapčević Hadnađev  Miroslav Hadnađev 《Journal of Cereal Science》2013

The main aim of this study was to determine the changes in free sulphydryl content during postharvest wheat and flour maturation. The content of free sulphydryl groups was determined from wet gluten over 50 days of wheat postharvest maturation and over 14 days of flour maturation by varying incubation temperatures (30 and 37 °C) and incubation times (0, 45, 90 and 135 min). The amount of free sulphydryl groups increased during postharvest wheat and flour maturation as well as with the increase in temperature and gluten incubation time. The additional aim of this study was to find the interrelation between the content of free sulphydryl groups and selected parameters of technological quality by means of Principal Component Analysis. During wheat/flour maturation, the strengthening of protein structure was observed manifested by the increase in gluten index and Mixolab protein network weakening (C2), and changes in dough resistance as well. The incubation temperature of 37 °C affected the weakening of protein structure manifested by the decrease in gluten index and resistance, and increase in extensibility. Strong differentiation of examined varieties in terms of selected quality indicators was observed upon pre-defined maturation period, probably due to the expression of their intrinsic properties.  相似文献   

Chloroplast DNA polymorphism of an exotic P. mugo Turra population introduced to seaside spit of Kursiu Nerija in Lithuania     

Darius Danusevičius  Jurata Buchovska  Vidmantas Stanys  Juratė Bronė Šikšnianienė  Virgilijus Baliuckas  Gediminas Brazaitis 《European Journal of Forest Research》2013,132(1):137-150

The aim of this study was to elucidate the introduction history of P. mugo in the unique landscape of the Lithuanian seaside spit of Kursiu Nerija by assessing its genetic structure and the genetic diversity. The individuals were sampled in 12 populations within an area of 3 km × 50 km along the Lithuanian part of Kursiu Nerija. P. mugo was introduced over 200 years ago to prevent sand erosion by establishing a forest cover. Chloroplast DNA polymorphism of 220 individuals of P. mugo together with 18 P. sylvestris and 11 putative P. sylvestris × P. mugo hybrids was assessed by the aid of five microsatellite markers. The standard intra-population diversity indexes were calculated. The intra-specific variation between distinct morphotypes as well as the population differentiation within the most spread P. mugo ssp. rotundata morphotype was assessed based on the haplotype frequencies by hierarchical AMOVA, G_ST/R_ST test, UPGMA clustering and PCA methods. The genetic diversity of P. mugo in Kursiu Nerija was high (H_e = 0.95; 83 different haplotypes). All except one of the P. mugo populations sampled contained a notable share of private haplotypes. AMOVA revealed high intra-specific diversity but low differentiation between the P. mugo populations. Most of the haplotypic variance was within populations. The UPGMA clustering produced groups more corresponding to the sub-species morphotypes than the geography of the populations. There was no geographical pattern of reduction in genetic diversity towards the younger plantations. A strong candidate for a species-specific DNA marker was found. After several events of introduction, the genetic diversity of P. mugo in Kursiu Nerija is very high and is structured based on the sub-species morphotypes rather than geography. The high frequency of shared and notable frequency of private haplotypes in most of the populations indicate that the major part of the P. mugo material originates from a number of geographically and genetically related sources, which more likely are introductions from abroad that the local collections. The high frequency of private haplotypes in the northernmost populations leaves a possibility for minor introductions from other genetically distinct sources. The absence of private haplotypes in one of the sampled populations indicates the use of local seed collections. The large number of shared haplotypes provides a strong evidence for a geneflow among the P. mugo taxa.  相似文献   

Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.)     

Dušan Jankov  Dušanka Inđić  Petar Kljajić  Radmila Almaši  Goran Andrić  Slavica Vuković  Mila Grahovac 《Journal of pest science》2013,86(2):211-216

The aim of the study was to investigate how various types of storage facilities with, e.g., concrete, metal, and plywood surfaces interfere with the activity of different insecticide formulations used for rice weevil Sitophilus oryzae (L.) control: malathion (EC), pirimiphos-methyl (EC), and lambda-cyhalothrin (CS and WP). Initial and residual efficacy were determined in the laboratory. Knockdown data for the initial effects were processed by probit analysis and presented as knockdown time (KDT) parameters with kdt-p lines. Delayed effects were shown as knockdown efficacy (%) determined after 24 h of weevils’ contact with 7-, 14-, 30-, 60-, 90-, 120-, 150-, and 180-day-old deposits on each surface. Malathion (EC) and pirimiphos-methyl (EC) showed the highest initial knockdown efficacy on metal, while it was 3.6 (3.4)- and 4.4 (3.3)-fold lower on concrete and plywood, respectively. Lambda-cyhalothrin (CS and WP) showed the highest initial efficacy on concrete, and slightly lower (1.3 and 2.4) fold on metal and plywood, respectively. Both formulations of lambda-cyhalothrin and malathion on metal, as well as pirimiphos-methyl on plywood were 100 % efficient against S. oryzae 180 days after the treatment. Delayed efficacy of both formulations of lambda-cyhalothrin decreased on plywood after 120 days, and after 180 days the efficacy was 55 %. All insecticides, except lambda-cyhalothrin (CS), expressed low knockdown efficacy on concrete, while the deposit of lambda-cyhalothrin (CS) on concrete was 100 % efficient during 90 days, and after 120, 150, and 180 days the efficacy was 83, 65, and 17 %, respectively.  相似文献   

Histochemical Distribution of Digestive Enzymes in the Intestine of the Common Two‐Banded SeaBream,Diplodus vulgaris,Geoffroy St‐Hilaire 1817     

I. Tlak Gajger  S. Nejedli  Z. Kozarić 《Anatomia, histologia, embryologia》2013,42(3):161-167

  相似文献   

Genotyping of Bacillus anthracis Isolated from Croatia and Bosnia and Herzegovina     

I. Steiner  I. Račić  S. Špičić  B. Habrun 《Zoonoses and public health》2013,60(3):202-208

Anthrax is a serious disease caused by Bacillus anthracis. Humans can become infected by handling products from infected animals, by breathing spores and rarely by eating undercooked meat from infected animals. The genome of B. anthracis is highly monomorphic and thus shows very low DNA sequence variation. We analysed the molecular characteristics of 12 B. anthracis isolates from outbreaks in Croatia and Bosnia and Herzegovina, which have occurred during the past 10 years along with two vaccine strains. Genotyping system based on variable‐number tandem repeat analysis at six loci revealed that six isolates belong to genotype from the A1.a cluster whilst six isolates relate to the B2 cluster, compared to 89 previously described genotypes. The distribution of two evolutionarily distant clusters suggests an introduction of B. anthracis to this area in at least two separate events.  相似文献   

Distribution and Genotypic Characterization of Campylobacter jejuni Isolated from Poultry in Split and Dalmatia County,Croatia     

A. Kovačić  I. Listeš  C. Vučica  L. Kozačinski  I. Tripković  K. Šiško‐Kraljević 《Zoonoses and public health》2013,60(4):269-276

Consumption of poultry contaminated with Campylobacter jejuni has been recognized worldwide as the leading cause of campylobacteriosis. Therefore, the aim of our study was to investigate the prevalence and genotype diversity of Campylobacter jejuni in poultry meat intended for consumption in Split and Dalmatia County, which is the second biggest County in Croatia. Furthermore, we also wanted to discover possibly stable clones of C. jejuni appearing in different samples and periods of time, which would indicate their ability to persist in or adapt to poultry. In the period from March 2008 until June 2010, 834 samples of poultry from various sources were examined using a surface swab technique. Isolation of C. jejuni was performed by Preston broth and Karmali agar. Identification of the isolates was carried out using biochemical tests. C. jejuni was found in 84 of 574 chicken samples (14.6%) and in nine of 260 samples of turkey (3.5%). Pulse‐field gel electrophoresis (PFGE) was used to analyse 61 obtained isolates using SmaI and KpnI. Of 22 different macrorestriction profiles (MRP) that were found, five were detected in poultry from both different locations and periods of time. Samples from 11 locations were found to be contaminated with more than two different genotypes of C. jejuni. Interestingly, the same MRP were found both in poultry declared to be of domestic origin and in the poultry imported from abroad. The prevalence of C. jejuni in poultry samples was in accordance with previously reported results. Genotypic analysis indicated that the population of C. jejuni in Split and Dalmatia County was diverse and that multiple strains of C. jejuni could be found in the same poultry samples. Furthermore, the same genotypes were identified from the samples obtained from different locations and periods of time, which could support the theory of a global existence of certain MRP that are able to persist in or adapt to poultry.  相似文献   

Effects of mycorrhizal fungi on root-hair growth and hormone levels of taproot and lateral roots in trifoliate orange under drought stress     

Fei Zhang  Peng Wang  Ying-Ning Zou  Kamil Kuča 《Archives of Agronomy and Soil Science》2019,65(9):1316-1330

A pot experiment was used to evaluate the effects of an arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae on plant growth performance, root-hair growth, and root hormone levels in trifoliate orange (Poncirus trifoliata) seedlings under well-watered (WW) and drought stress (DS). A 9-week mild DS treatment significantly reduced mycorrhizal colonization of 2nd- and 3rd-order lateral roots. Root mycorrhizal colonization was relatively higher in the 2nd- and 3rd-order lateral roots than in the taproot and the 1st-order lateral root under WW and DS. AMF seedlings exhibited significantly higher root-hair density, length (except for the taproot) and diameter in taproot and 1st-, 2nd-, and 3rd-order lateral roots under WW, and considerably higher root-hair density (except for 1st-order lateral root), length (except for 2nd-order lateral root) and diameter under DS. Mycorrhizal inoculation remarkably increased root abscisic acid (ABA), indole-3-acetic acid (IAA), methyl jasmonate, and brassinosteroids (BRs) concentrations under DS, in company with the decrease in root zeatin riboside and gibberellins levels and root IAA effluxes. Root-hair traits were significantly positively correlated with root colonization and root ABA and BRs levels. It is concluded that mycorrhizal plants possessed better root-hair growth to adapt mild DS, which is associated with mycorrhizal colonization and endogenous hormone changes.  相似文献