Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding     

Zhou M  Morgner N  Barrera NP  Politis A  Isaacson SC  Matak-Vinković D  Murata T  Bernal RA  Stock D  Robinson CV 《Science (New York, N.Y.)》2011,334(6054):380-385

The ability of electrospray to propel large viruses into a mass spectrometer is established and is rationalized by analogy to the atmospheric transmission of the common cold. Much less clear is the fate of membrane-embedded molecular machines in the gas phase. Here we show that rotary adenosine triphosphatases (ATPases)/synthases from Thermus thermophilus and Enterococcus hirae can be maintained intact with membrane and soluble subunit interactions preserved in vacuum. Mass spectra reveal subunit stoichiometries and the identity of tightly bound lipids within the membrane rotors. Moreover, subcomplexes formed in solution and gas phases reveal the regulatory effects of nucleotide binding on both ATP hydrolysis and proton translocation. Consequently, we can link specific lipid and nucleotide binding with distinct regulatory roles.  相似文献   

Ecology and distribution of Armillaria species in Norway     

N. Keča  H. Solheim 《Forest Pathology》2011,41(2):120-132

The occurence of Armillaria species was assessed in Norway, enabling the northern‐most distribution of this genus to be determined in Europe. Four Armillaria species were found in Norway. Armillaria borealis was the most common species occurring on woody vegetation to the permafrost zone (ca. 69°N). Armillaria cepistipes was present in southern and central Norway, but was not found further than 66°N. Armillaria solidipes and Armillaria gallica were rare, found at only one locality each; 59°40′ and 59°32′, respectively. Armillaria species were found on 14 hosts, but there was no significant difference between occurrence of A. borealis and A. cepistipes on declining and dead trees. Phylogenetic analyses separated each species into separate clades. All isolates of A. borealis, except one, and most isolates of A. solidipes were in separate clades. However, a subclade within the A. borealis clade was formed of two A. ostoyae and one A. borealis isolates. Two small A. cepistipes genets were found in a declining oak stand.  相似文献   

Congenital diaphragmatic eventration in two closely related British Shorthair cats     

Gombač M  Vrecl M  Svara T 《Journal of Feline Medicine and Surgery》2011,13(4):276-279

Two closely related British Shorthair cats, which died after surgery performed in the dorsal position, were presented for dissection. In both, a thin, flaccid, enlarged transparent tendinous diaphragmatic portion protruded cranially into the thoracic cavity, forming a cupola in which left, right medial and quadrate hepatic lobes were encased in both cats and the stomach in one cat. Microscopically, no muscle fibres were observed in the membrane, but numerous hepatocytes and bile ducts were incorporated in its central part. The anomaly was diagnosed as a congenital diaphragmatic eventration.  相似文献   

Distribution of Potato virus Y in potato plant organs, tissues, and cells     

Kogovšek P  Kladnik A  Mlakar J  Znidarič MT  Dermastia M  Ravnikar M  Pompe-Novak M 《Phytopathology》2011,101(11):1292-1300

The distribution of Potato virus Y (PVY) in the systemically infected potato (Solanum tuberosum) plants of the highly susceptible cultivar Igor was investigated. Virus presence and accumulation was analyzed in different plant organs and tissues using real-time polymerase chain reaction and transmission electron microscopy (TEM) negative staining methods. To get a complete insight into the location of viral RNA within the tissue, in situ hybridization was developed and optimized for the detection of PVY RNA at the cellular level. PVY was shown to accumulate in all studied leaf and stem tissues, in shoot tips, roots, and tubers; however, the level of virus accumulation was specific for each organ or tissue. The highest amounts of viral RNA and viral particles were found in symptomatic leaves and stem. By observing cell ultrastructure with TEM, viral cytoplasmic inclusion bodies were localized in close vicinity to the epidermis and in trichomes. Our results show that viral RNA, viral particles, and cytoplasmic inclusion bodies colocalize within the same type of cells or in close vicinity.  相似文献   

Influence of land use in small karst watersheds on the chemical status of peloid sediments on the eastern Adriatic coast     

Slobodan Miko  Georg Koch  Saša Mesić  Martina Šparica Miko  Marko Šparica  Polona Vreča  Tadej Dolenec 《Journal of Soils and Sediments》2007,7(5):303-312

Background, Aim and Scope  

Most of the cathment areas supplying water and mineral matter to the Adriatic bays with healing mud/peloids are affected by various degrees of human impact, i.e. activity that is introducing various types of contaminants to environment. The bays represent shallow marine depositional environments where organic-rich sediments accumulate. Investigations for balneological characterization of several localities along the Croatian coast have shown that these organic-rich sediments may be classified as healing mud, i.e. peloids. Healing mud due to its balneologic features is in treatment of various diseases and in improvement of the overall health conditions and general well-being. Various components of mud, particularly trace elements, could be absorbed through dermal contact. Therefore, the study of the abundance of such potentially toxic elements as As, Pb, Hg, Cd, Se, Sb, Cu, Zn in marine sediments used as healing mud is necessary in order to know their mobility and to avoid possible intoxications.  相似文献   

Organic-geochemical Differentiation of Petroleum-type Pollutants and Study of Their Fate in Danube Alluvial Sediments and Corresponding Water (Pančevo Oil Refinery, Serbia)     

Branimir Jovančićević  Miroslav Vrvić  Jan Schwarzbauer  Hermann Wehner  Georg Scheeder  Dragomir Vitorović 《Water, air, and soil pollution》2007,183(1-4):225-238

A review is given in this paper of the up-to-date results observed in differentiation and transformation studies on petroleum-type pollutants in underground and surface waters. Water and particulate matter derived from the locality of Pan?evo Petroleum Refinery, Serbia (River Danube alluvial formations). It was shown that distributions of n-alkanes, steranes and triterpanes, and δ¹³C_PDB values of n-alkanes may successfully be used for qualitatively differentiating the petroleum-type pollutants from native organic matter in recent sedimentary formations. In underground waters, a petroleum-type pollutant is exposed to microbiological degradation which is manifested through relatively fast degradation of n-alkanes. Following an almost complete degradation of crude oil n-alkanes in underground water, the biosynthesis of novel, even carbon-number C₁₆–C₃₀ n-alkanes may be observed. It is shown that the n-alkane distribution observed in a petroleum-type pollutant may depend on the intensity of its previous interaction with water. The fate of petroleum-type pollutants in environmental waters may be predicted through laboratory simulative microbiological degradation experiments by using microorganism consortiums similar to those observed under relevant natural conditions, as well as on corresponding nutrient base.  相似文献   

Spatial and vertical distribution of <Superscript>137</Superscript>Cs in soils in the erosive area of southeastern Serbia (Pčinja and South Morava River Basins)     

Jelena Petrović  Snežana Dragović  Ranko Dragović  Milan Đorđević  Mrđan Đokić  Mirjana Ćujić 《Journal of Soils and Sediments》2016,16(4):1168-1175

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Effects of root exudates and humic substances on weathering kinetics     

Michael Ochs  Ivano Brunner  Werner Stumm  BoŽena ćosović 《Water, air, and soil pollution》1993,68(1-2):213-229

The effect of complex natural organic ligands on the weathering kinetics of aluminum oxide was investigated in laboratory experiments. A peat-derived humic substance and root exudates obtained from ectomycorrhizal (Picea abies — Hebeloma crustuliniforme) and non-mycorrhizal Norway Spruce trees; and γ-Al₂O₃ were used as a model system. The experimental weathering rates are in accordance with a surface-controlled dissolution mechanism. The effect of the humic material on dissolution rates appears to depend on the degree of protonation of the humic (macro)molecules: we observed dissolution-enhancement or -inhibition at pH 3 and 4, respectively. Ectomycorrhizal exudates proved to be effective weathering agents at pH 4, as opposed to humic material and non-mycorrhizal exudates. Our results suggest that (i) the role of humic materials in mineral weathering and podzolization is different from what is commonly thought, and (ii) mineral weathering rates in the rhizosphere may be higher than in the bulk soil.  相似文献   

A procedure for evaluating and mapping the erosion resistance of soils (by the example of irrigated soils of Tajikistan)     

O. A. Bazarov  V. Ya. Grigor’ev  O. V. Solov’eva 《Eurasian Soil Science》2006,39(11):1238-1247

A procedure was developed on the basis of the experimental-theoretical equation for calculating the parameters of soil erosion resistance (the bottom eroding velocity of flow), the principles of the erosion resistance theory, the experimentally obtained relationships between the main erosion parameters of Tajikistan soils (the size of water-stable aggregates and their cohesion), and other properties (the initial water content, the lumpiness, the contents of physical clay and humus, the porosity ratio, and the lower plasticity limit). For practical purposes, a simpler procedure is recommended to evaluate and map the erosion resistance of irrigated lands in the areas of typical serozems and mountain cinnamonic soils of Tajikistan from minimum initial information (the texture, lumpiness, and density of the soils and the content of plant roots).  相似文献   

Principal component analysis of trace elements in Serbian wheat     

Skrbić B  Durisić-Mladenović N  Cvejanov J 《Journal of agricultural and food chemistry》2005,53(6):2171-2175

Trace elements (Cu, Fe, Pb, Hg, Cd, As, Mn, Zn) were analyzed quantitatively in 14 wheat samples collected from fields in all Serbian growing regions, harvested in 2002. Microelements were determined according to an atomic absorption spectrophotometric method. Principal component analyses (PCA) were performed on data matrices consisting of contents of trace elements in wheats (columns) and all Serbian wheat-growing regions (rows). It was found that four principal components account for 87.2% of the total variance in the data. The plot of component loadings showed significant groupings for concentration of some microelements. The component scores indicated the similarities among the Serbian wheat-growing regions. The loading plot reveals that there is no need to measure all of the variables to achieve the same classification. It is enough to measure one variable per group. Naturally, this conclusion is valid only within the limits of the present study of wheat grain samples from different parts of Serbia.  相似文献