Apoptogenic Metabolites in Fractions of the Benthic Diatom Cocconeis scutellum parva     

Valerio Zupo  Friedrich Jüttner  Chingoileima Maibam  Emanuela Butera  Judith F. Blom 《Marine drugs》2014,12(1):547-567

Benthic diatoms of the genus Cocconeis contain a specific apoptogenic activity. It triggers a fast destruction of the androgenic gland in the early post-larval life of the marine shrimp Hippolyte inermis, leading to the generation of small females. Previous in vitro investigations demonstrated that crude extracts of these diatoms specifically activate a dose-dependent apoptotic process in human cancer cells (BT20 breast carcinoma) but not in human normal lymphocytes. Here, a bioassay-guided fractionation has been performed to detect the apoptogenic compound(s). Various HPLC separation systems were needed to isolate the active fractions, since the apoptogenic metabolite is highly active, present in low amounts and is masked by abundant but non-active cellular compounds. The activity is due to at least two compounds characterized by different polarities, a hydrophilic and a lipophilic fraction. We purified the lipophilic fraction, which led to the characterization of an active sub-fraction containing a highly lipophilic compound, whose molecular structure has not yet been identified, but is under investigation. The results point to the possible medical uses of the active compound. Once the molecular structure has been identified, the study and modulation of apoptotic processes in various types of cells will be possible.  相似文献   

Evaluation of Trace Metal Fluxes to Soils in Hinterland of Porto Marghera Industrial Zone: Comparisons with Direct Measurements in the Lagoon of Venice     

Scazzola  Roberto  Matteucci  Gabriele  Guerzoni  Stefano  Chiamenti  Elisa  Rossini  Paolo  Molinaroli  Emanuela 《Water, air, and soil pollution》2004,153(1-4):195-203

Trace metal (As, Cd, Cr, Ni, Hg, Pb) concentrations in soil samples collected around the Porto Marghera (Italy) industrial zone (2–40 km) near the city of Venice were compared with direct measurements of atmospheric deposition measured at comparable distances from the same source. Concentrations of Cd and Pb in soils decreased exponentially with increasing distance from the source; less clear signals were detected for As, Cr and Ni. Significant differences were found among the soils, which were partially resolved when their clay contents were normalised. Preliminary comparisons of fluxes of Cd and Pb derived from soil with direct deposition measurements showed increasing values with longer integration times. Annual Cd and Pb flux values of one year of direct deposition were 5 to 10 times lower than mean annual fluxes derived from soils,integrating 50 to 100 years. Values range from ～0.1–0.4 to 1–2 mg m^-2 yr^-1 for Cd, and from 3–18 to 50–100 mg m^-2 yr^-1 for Pb. These results fit information on `historical' emission trends as recorded in sediments of the lagoon.  相似文献   

TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity     

Caputo A  Caci E  Ferrera L  Pedemonte N  Barsanti C  Sondo E  Pfeffer U  Ravazzolo R  Zegarra-Moran O  Galietta LJ 《Science (New York, N.Y.)》2008,322(5901):590-594

Calcium-dependent chloride channels are required for normal electrolyte and fluid secretion, olfactory perception, and neuronal and smooth muscle excitability. The molecular identity of these membrane proteins is still unclear. Treatment of bronchial epithelial cells with interleukin-4 (IL-4) causes increased calcium-dependent chloride channel activity, presumably by regulating expression of the corresponding genes. We performed a global gene expression analysis to identify membrane proteins that are regulated by IL-4. Transfection of epithelial cells with specific small interfering RNA against each of these proteins shows that TMEM16A, a member of a family of putative plasma membrane proteins with unknown function, is associated with calcium-dependent chloride current, as measured with halide-sensitive fluorescent proteins, short-circuit current, and patch-clamp techniques. Our results indicate that TMEM16A is an intrinsic constituent of the calcium-dependent chloride channel. Identification of a previously unknown family of membrane proteins associated with chloride channel function will improve our understanding of chloride transport physiopathology and allow for the development of pharmacological tools useful for basic research and drug development.  相似文献   

Management of Chronic Diarrhea in an Adult Horse     

Emanuela Valle  Marco Gandini  Domenico Bergero 《Journal of Equine Veterinary Science》2013

Chronic diarrhea could be a challenge from both the diagnostic and therapeutic point of view. This case report will discuss the clinical approach to the evaluation of chronic diarrhea with the production of free fecal water in an adult horse. The discussion of this case report may provide further information and describe a possible therapeutic option with sulfasalzine, focusing on the nutritional management of chronic diarrhea in adult horses. Nutritional management could be of higher importance for horses that suffer from this problem because it improves the well-being of the hindgut environment. In the present case report, motility abnormalities were identified without other physiological disturbances of colonic function or a decrease in body weight, and the priority was the following: (1) rebalancing of the diet to the real nutritional requirements of the patient, (2) avoiding excess carbohydrate from both forage and concentrate, (3) selecting the right proportion between good quality first-cut meadow hay and grounded and pelleted meadow hay that helps to reduce mechanical and physical load of the colon and could help in the formation of more homogeneous digesta, (4) reducing the stress with adequate feed consumption time, meal size, and time spent in a overgrazed paddock, and (5) increasing the proportion of omega-3 fatty acids in the diet. With the nutritional plan and management and the initial use of sulfasalazine, the horse maintained a good quality of the feces with a reduced or absent production of the free fecal water.  相似文献   

Carbon and nitrogen mineralization in soil of leguminous trees in a degraded pasture in northern Rio de Janeiro,Brazil     

Danielle A. D. Nunes  Emanuela F. Gama-Rodrigues  Patrícia A. B. Barreto  Antonio C. Gama-Rodrigues  Paulo H. M. Monroe 《林业研究》2016,27(1):91-99

Use of legume trees can improve soil quality in degraded pastures. The aim of this study was to characterize C and N mineralization kinetics and estimate the potentially mineralizable C and N in soil under Mimosa caesalpiniifolia Benth. and Acacia auriculiformis A. Cunn.ex Benth. secondary forest and pasture in red-yellow latosols in southeast Brazil. We conducted a laboratory aerobic incubation experiment using a completely randomized design of four replicates and four types of plant cover using a modified version of the Stanford and Smith technique(1972) to study C and N mineralization potential.Potentially mineralizable N(N_o) ranged from 135 to170 mg kg-~(1). The predominant form of mineral N for all types of plant cover was N-NO_3~-. M. caesalpiniifolia was the only species that had a positive influence on N mineralization. Neither of the legumes influenced C mineralization in pasture or secondary forest. The model of N mineralization corresponded to a sigmoidal curve while C mineralization corresponded to an exponential curve,revealing that the N and C mineralization processes were distinct. N mineralized by M. caesalpiniifolia(216 kg of N ha~(-1)) was adequate to meet the N requirement for a livestock-forest system.  相似文献   

A case of feline injection-site sarcoma at the site of cisplatin injections     

Martano M  Morello E  Iussich S  Buracco P 《Journal of Feline Medicine and Surgery》2012,14(10):751-754

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Molecular characterization of a phytoplasma causing Phyllody in Clover and other herbaceous hosts in Northern Italy     

Giuseppe Firrao  Luigi Carraro  Emanuela Gobbi  Romano Locci 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(9):817-822

Red clover (Trifolium pratense) and Ladino clover (Trifolium repens) plants showing phytoplasma-associated symptoms (yellowing/reddening, virescence and phyllody) have been recovered in Friuli-Venezia Giulia, Italy. Using AluI RFLP analysis of PCR amplified 16S rDNA we showed that the disease can be caused independently by two phylogenetically distinct phytoplasmas. One of them showed the very typical 16S rDNA RFLP pattern of the agent of Clover Phyllody in Canada (CCPh). The 16S rDNA of the other phytoplasma (Italian Clover Phyllody phytoplasma, ICPhp) has been PCR amplified, cloned and sequenced. The sequence revealed high similarity (>98%) with phytoplasmas belonging to the X disease cluster, which includes organisms not reported to cause phyllody on their hosts. The analysis by AluI RFLP of the PCR amplified pathogen 16S rDNA from other herbaceous plants (Crepis biennis, Taraxacum officinale, Leucanthemum vulgare) collected nearby with phytoplasma-associated symptoms showed similar patterns. Southern blot hybridization of their EcoRI digested total DNA revealed identical RFLP patterns, suggesting that the causative agent may be the same organism.Abbreviations PCR Polymerase Chain Reaction - rDNA gene for the small subunit ribosomal RNA - RFLP Restriction Fragment Length Polymorphism  相似文献   

Patterns of nodal metastases,biological behaviour and prognosis of canine mast cell tumours of the pinna: A multi-institutional retrospective study     

Carmit Chalfon  Riccardo Finotello  Silvia Sabattini  Irina Gramer  Joanna Siriol Morris  Marina Aralla  Emanuela Maria Morello  Erica Ferraris  Sofia Ramos  Gerry Polton  Luca Schiavo  Jane Dobson  Veronica Cola  Laura Marconato 《Veterinary and comparative oncology》2023,21(2):332-338

Canine cutaneous mast cell tumours (cMCTs) of the pinna have been associated with an aggressive biological behaviour, although data remain scarce. The knowledge acquired over the past years on histologic gradings, and the value of lymph node (LN) staging, may help in better characterizing this anatomical presentation. The first aim was to describe the frequency, location, and histologic appearance of LN metastases in cMCT of the pinna. A second aim was to evaluate prognosis. Medical records of dogs with cMCT of the pinna, that underwent tumour and sentinel (SLN) or regional LN (RLN) excision, were reviewed. The influence of potential prognostic variables on time to progression (TTP) and tumour-specific survival (TSS) was investigated. Thirty-nine dogs were included: 19 (48.7%) had Kiupel high-grade (K-HG) and 20 (51.3%) had low-grade (K-LG) MCTs. Eighteen (46.1%) dogs underwent SLN mapping: the superficial cervical LN was at least one of SLN in 17 (94.4%) cases. Twenty-two (56.4%) dogs had LN metastases; the superficial cervical LN was always involved. On multivariable analysis, only K-HG was associated with increased risk of progression (p = .043) and tumour-related death (p = .021). Median TTP and TSS were 270 and 370 days in K-HG, respectively; these were not reached in dogs with K-LG tumours (p < .01). cMCTs of the pinna are often K-HG and are also associated with a higher frequency of LN metastasis; however, we confirmed the independent prognostic value of histologic grading. A multimodal treatment may lead to favourable long-term outcome. Moreover, the superficial cervical LN is most often the SLN.  相似文献   

Cold‐water coral Madrepora oculata in the eastern Ligurian Sea (NW Mediterranean): Historical and recent findings          下载免费PDF全文

Emanuela Fanelli  Ivana Delbono  Roberta Ivaldi  Marta Pratellesi  Silvia Cocito  Andrea Peirano 《水产资源保护:海洋与淡水生态系统》2017,27(5):965-975

Posidonia oceanica (L.) Delile Dampens Cell Migration of Human Neuroblastoma Cells     

Marzia Vasarri  Manuela Leri  Emanuela Barletta  Carlo Pretti  Donatella Degl&#x;Innocenti 《Marine drugs》2021,19(10)

Neuroblastoma (NB) is a common cancer in childhood, and lethal in its high-risk form, primarily because of its high metastatic potential. Targeting cancer cell migration, and thus preventing metastasis formation, is the rationale for more effective cancer therapy against NB. Previous studies have described the leaf extract from Posidonia oceanica marine plant (POE) as an antioxidant, anti-inflammatory agent and inhibitor of cancer cell migration. This study aims to examine the POE anti-migratory role in human SH-SY5Y neuroblastoma cells and the underlying mechanisms of action. Wound healing and gelatin zymography assays showed that POE at early times inhibits cell migration and reduces pro-MMP-2 release into culture medium. By monitoring expression level of key autophagy markers by Western blot assay, a correlation between POE-induced cell migration inhibition and autophagy activation was demonstrated. Cell morphology and immunofluorescence analyses showed that POE induces neurite formation and neuronal differentiation at later times. These results suggest POE might act against cell migration by triggering early nontoxic autophagy. The POE-induced cellular morphological change toward cell differentiation might contribute to prolonging the phytocomplex anti-migratory effect to later times. Overall, these results encourage future in vivo studies to test POE applicability in neuroblastoma treatment.  相似文献