Differential gene expression and apoptosis markers in presymptomatic scrapie affected sheep     

Hedman C  Lyahyai J  Filali H  Marín B  Serrano C  Monleón E  Moreno B  Zaragoza P  Badiola JJ  Martín-Burriel I  Bolea R 《Veterinary microbiology》2012,158(1-2):23-32

Bluetongue viruses (BTVs) could invade N-W Europe similar to BTV serotype 8 (BTV8/net06), since the source and route of introduction of this virus has not been solved. Therefore, the Dutch survey for Bluetongue by PCR testing was extended by further analysis of PCR positives to identify the involved BTV. In late August 2008, BTV was reported with 12 nucleotide differences in the S10 amplicon (S10 genotyping). This virus was identified as serotype 6, here named BTV6/net08. Promptly, serotype specific real-time PCR tests were developed for serotypes 1, 6, and 8 (S2 genotyping). Agreement was found between results by S10- and S2 genotyping. Further, BTV1 was identified by both S10- and S2 genotyping in one imported animal. After initial discovery of BTV6 in the Netherlands, animals from 18 holdings tested PCR positive for BTV6/net08 in 2008. Remarkably only one or two PCR positive animals per holding were found. Serum neutralization tests did not result in the discovery of more BTV6 infected animals. Retrospective studies indicated no evidence for infections by BTV6/net08 prior to the first discovery. Experimental infections with BTV6/net08 did not cause clinical disease in sheep, calves and cattle, except for a very short fever in some animals. This clearly showed that the vaccine-related BTV6/net08 is not virulent. BTV6/net08 was not found by passive and active surveys in the years after its discovery. Apparently, BTV6/net08 was not efficiently transmitted by endemic species of Culicoides in N-W Europe, and disappeared without the need of any control measure.  相似文献   

An artificial inoculation protocol for Uromycladium acaciae,cause of a serious disease of Acacia mearnsii in southern Africa     

Stuart Fraser  Alistair R McTaggart  Julian Moreno Chan  Thobile Nxumalo  Louise S Shuey  Michael J Wingfield 《Southern Forests》2019,81(1):85-90

Uromycladium acaciae is the cause of a severe wattle rust epidemic in plantations of Acacia mearnsii (black wattle) in southern Africa. Research on the biology of this damaging rust is assisting in the development of control strategies. One strategy under investigation is the identification and deployment of resistant lines of A. mearnsii. Selection of resistant families currently relies on large-scale, time-consuming and expensive field trials. In this study, we present a detailed artificial inoculation protocol for U. acaciae, which can be used to screen for resistance. The results of an experiment that used the protocol to screen the relative resistance of 12 families of A. mearnsii to U. acaciae are also presented. The developed artificial inoculation protocol can also be used to investigate several other aspects of this host–pathogen system.  相似文献   

A soil‐quality index for soil from Mediterranean forests     

Manuela Andrs‐Abelln  Consolacin Wic‐Baena  Francisco R. Lpez‐Serrano  Francisco A. García‐Morote  Eduardo Martínez‐García  Marta I. Picazo  Eva Rubio  Jose L. Moreno‐Ortego  Felipe Bastida‐Lpez  Carlos García‐Izquierdo 《European Journal of Soil Science》2019,70(5):1001-1011

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The use of infrared spectroscopy and machine learning tools for detection of Meloidogyne infestations     

Ernesto San-Blas  Gabriel Paba  Néstor Cubillán  Edgar Portillo  Ana M. Casassa-Padrón  César González-González  Mayamarú Guerra 《Plant pathology》2020,69(8):1589-1600

Plant parasitic nematodes are generally soilborne pathogens that attack plants and cause economic losses in many crops. The infested plants show nonspecific symptoms or, often, are symptomless; therefore, diagnosis is performed by taking soil and root tissue samples. Here, we show that a combination of different infrared spectra analysis and machine learning algorithms can be used to detect plant parasitic nematode infestations before symptoms become visible, using leaves instead of roots and soil as samples. We found that tomato and guava plants infested with Meloidogyne enterorlobii produced different spectral patterns compared to uninfested plants. Using partial spectra from 1,450 to 900/cm as the "fingerprint region", principal component analyses indicated that after 5 (tomatoes) or 8 weeks (guava), plants with no visible symptoms of infestations were positively diagnosed. To improve the early detection response, we used machine learning modelling. A support vector machine (SVM) was used to obtain more robust, accurate models. The SVM model contained 34 support vectors, 17 for each level. The overall performance of the model was >97% and the total accuracy was significantly higher, demonstrating the absence of chance prediction. The best prediction of infestation was obtained at the second and fourth weeks for tomatoes and guavas, respectively, reducing the diagnostic time by half. The combined application of these techniques reduces the processing time from field to laboratory and shows enormous advantages by avoiding root and soil sampling.  相似文献   

Organic trace minerals on productive performance,egg quality and immune response in Bovans White laying hens     

David Ramos‐Vidales  Gabriela Gmez‐Verduzco  Arturo Cortes‐Cuevas  Juan Carlos del Río‐García  Sergio Fernndez‐Tinoco  Silvestre Chrraga‐Aguilar  Ernesto vila‐Gonzlez 《Journal of animal physiology and animal nutrition》2019,103(5):1484-1491

This study was performed in 360 laying Bovans White hens and aimed to evaluate the effect of Carbo‐Amino‐Phosphate‐Chelates (CAFQ) as a mineral premix and its 30% reduction by compared to inorganic trace minerals (ITM) concerning its performance, egg quality, tibia breaking strength and immunity. Sixty‐week‐old hens were assigned into three treatments with 10 replicates of 12 birds each. Treatments were as follows: (a) (100% ITM), (b) (100% CAFQ) and (c) (70% CAFQ). Based on the results obtained during 12 weeks, under the current test conditions, improved yolk colour (p < 0.05); shell breaking strength (p < 0.05); and storage time (p < 0.05) were observed in hens fed with a diet reduced by 70% in CAFQ inclusion showed similar performance behaviour and better egg breaking strength regarding with ITM, suggesting the possibility of minimizing the inclusion of trace minerals in laying hen diets improving environmental impact, in contrast to the use of inorganic sources of minerals.  相似文献   

Trends in the quality and hygiene parameters of bulk Italian Mediterranean buffalo (Bubalus bubalis) milk: A three year study          下载免费PDF全文

Marina Pasquini  Andrea Osimani  Stefano Tavoletti  Itzhak Moreno  Francesca Clementi  Maria Federica Trombetta 《Animal Science Journal》2018,89(1):176-185

Buffalo milk represents an indispensable source of nourishment in many parts of the world and it is the second most consumed milk worldwide. Buffalo milk is actually used for the production of many dairy products such as pasteurized or concentrated milk, butter, yogurt, ice‐cream, dehydrated milk products and cheeses. Due to its high nutritional value and the presence of natural bioactive substances, buffalo milk can also provide health benefits to consumers. In Italy, buffalo milk is used mainly for cheese making, mozzarella PDO (Protected Designation of Origin), which is a highly valued dairy product. This 3‐year study, carried out between 2011 and 2013, was aimed at evaluating the quality of bulk Italian Mediterranean buffalo milk by monitoring physico‐chemical parameters, somatic cell and total bacterial counts. A total of 51 samples of bulk milk were collected from one herd throughout the monitored period. Analysis of variance, carried out to test month, season and year main effects, highlighted remarkable seasonal effects for fat, protein and lactose content, as well as for predicted mozzarella cheese yield, and somatic cell counts. The calculation of simple correlations allowed the identification of positive correlations between estimated cheese yield and fat and protein content.  相似文献   

Exploring the Role of Management in the Coproduction of Ecosystem Services from Spanish Wooded Rangelands     

Mario Torralba  Elisa Oteros-Rozas  Gerardo Moreno  Tobias Plieninger 《Strength and Conditioning Journal》2018,71(5):549-559

The wood pastures or hardwood rangelands of the southwestern Iberian Peninsula are complex social-ecological systems created from the long-term interaction of society and the landscape. Dehesa, oak woodlands managed for grazing, cropping, and other forms of production, is the most common rangeland system and one of the most distinctive landscapes. Traditionally characterized by multifunctional low-intensity management that enhances a wide range of ecosystem services, current management has shifted from the traditional toward more intensified models. This paper assesses the coproduction of ecosystem services on dehesa properties by exploring the relationship between biophysical and sociocultural factors and dehesa management practices. Based on 42 surveys we analyze 16 quantitative indicators using multivariate techniques. The results indicate that there are four main dehesa types as defined by their characteristics and management: large heterogeneous operations with diverse products; small and homogeneous operations focused on a reduced number of products; medium-large properties focused on crop production; and midsized properties with easy public access. Management is the result of the dynamics of interacting biophysical and sociocultural factors that influence manager priorities and investments. Management decisions group around the degree of multifunctionality of the operation, the relative importance of crop production, the degree of grazing pressure in the system, and how restrictive public access policy is. We find that in the study area, interactions between all the previously mentioned elements covary consistently, generating bundles of ecosystem services associated with each of three management models based on the intensity of management.  相似文献   

Toxocara canis: proteinases in perivitelline fluid from hatching eggs     

González-Páez GE  Argüello-García R  Alba-Hurtado F 《Veterinary parasitology》2007,147(3-4):332-335

Developing larvae of Toxocara canis may secrete several kinds of enzymes within the egg perivitelline fluid (EPF) prior to and during hatching. In particular, proteinases in EPF could play a role in larval emergence within the host gastrointestinal lumen but its presence and nature is unknown. In this work, proteolytic activities in hatching fluid of T. canis were identified and analysed by substrate gel electrophoresis at different pH values and by using type specific protease inhibitors. Three bands of 91, 68 and 38 kDa showed gelatinolytic activity and all proteinase activity from EPF was of the aspartic-type since it was inhibited by pepstatin A. Interestingly, a significantly higher proteolytic activity was observed at acidic pH (< or =5.5). These data suggest that T. canis developmentally secretes and accumulates in EPF aspartic proteinases with a pH-dependent activity that might help the parasite to take advantage of conditions in the host gastrointestinal microenvironment where egg hatching is induced and executed.  相似文献   

Earthworm communities in native savannas and man-made pastures of the Eastern Plains of Colombia     

J. J. Jiménez  A. G. Moreno  T. Decaëns  P. Lavelle  M. J. Fisher  R. J. Thomas 《Biology and Fertility of Soils》1998,28(1):101-110

 The structure and seasonal changes of earthworm communities were evaluated in a natural savanna and in a improved grass-legume pasture in a Colombian oxisol over a period of 18 months. One plot of 90×90 m was isolated in each of the systems and each month five samples of 1 m²×0.5 m and ten of 20×20×20 cm were randomly selected from a stratified block design. Species richness was similar in the two evaluated plots (seven species), whereas diversity measured by the index, H (Shannon and Weaver 1949) was clearly different, i.e. H=2.89 in natural savanna and H=1.29 in pasture. This is explained by differences in earthworm community structure. The average annual density in the savanna was 49.8, ranging from 10.8 to 135.8 individuals (ind) m^–2, and biomass was 3.3 g m^–2 (hand-sorting method), ranging from 0.9 to 11.5 g m^–2. In the man-made pasture, density was 80.1 ind m^–2 on average, ranging from 24 to 215.8 ind m^–2 and biomass was more than tenfold higher, ranging from 29.2 to 110.4 g m^–2. This was especially due to the presence of a large glossoscolecid anecic species, Martiodrilus carimaguensis Jiménez and Moreno, which has been greatly favoured by conversion of savanna to pasture. Endogeic species were dominant in the natural savanna whereas the anecic species accounted for 88% of total earthworm biomass in the pasture. Total earthworm density and biomass were significantly different in the two systems studied (t-test). The results indicate a clearly positive response of earthworm communities to improved pastures, a type of land use that is being increasingly adopted in moist neotropical savannas. Received: 20 October 1997  相似文献   

Enhanced resistance to bacterial infection byErwinia carotovora subsp.atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes     

Patricio Arce  Mauricio Moreno  Mónica Gutierrez  Marlene Gebauer  Paola Dell’Orto  Hebert Torres  Ivette Acuña  Pauline Oliger  Alejandro Venegas  Xavier Jordana  Julio Kalazich  Loreto Holuigue 《American Journal of Potato Research》1999,76(3):169-177

Blackleg and soft rot diseases, caused by the bacteriumErwinia carotovora, are among the diseases that cause important losses in culture and storage of potato. In this paper, we introduced bacterial resistance into potato, via genes encoding for proteins with antibacterial activity. For this purpose, potato clones were transformed either with the gene encoding the acidic attacin protein fromHyalophora cecropia, or with the gene encoding the cecropin analog peptide SB37. These clones were evaluated for soft rot and blackleg resistance, after inoculation with the bacterial strainErwinia carotovora subsp.atroseptica T7. Results reported in this paper indicate that a considerable percentage of the potato clones (15–22%) showed increased resistance to bacterial infection, revealed by reduced severity of blackleg or soft rot symptoms. Expression of the transgenes was demonstrated in some of the clones by Northern blot analysis. This is the first report indicating that expression of the gene encoding for an attacin protein and for the cecropin SB-37 peptide in transgenic potato confers increased resistance to bacterial infection.  相似文献