Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets   总被引：1，自引：0，他引：1  

Kim HJ  Park JG  Alfajaro MM  Kim DS  Hosmillo M  Son KY  Lee JH  Bae YC  Park SI  Kang MI  Cho KO 《Veterinary microbiology》2012,159(1-2):11-22

Rotaviruses are important human and animal pathogens with high impact on public health and livestock industry. There is little evidence about the cross-species pathogenicity and extra-intestinal infections of animal and human reassortant rotaviruses, particularly based on all 11 genotyping data. In this study, the bovine triple reassortant KJ56-1 strain harboring two bovine-like genome segments, eight porcine-like genome segments, and one human-like genome segment was used to evaluate the cross-species pathogenicity in its parent species, calves and piglets, and to determine its abilities of causing viremia and extra-intestinal tropisms in piglets. The KJ56-1 strain isolated from a calf diarrhea fecal sample replicated without causing diarrhea and severe intestinal pathology in calves. However, piglets inoculated with this strain showed persistent severe diarrhea and marked intestinal pathology. By SYBR Green real-time RT-PCR, viral RNA was detected in the sera, mesenteric lymph node, lung, liver, choroid plexus, and cerebrospinal fluid in the experimental piglets. An immunofluorescence assay confirmed viral replication in these extra-intestinal organs and tissues. These results indicated that the bovine triple reassortant KJ56-1 strain was virulent to piglets but not to calves. Our data also demonstrated that the reassortant rotaviruses had the ability to spread to the bloodstream from the gut, enter and amplify in the mesenteric lymph node, and disseminate to the extra-intestinal organs and tissues.  相似文献   

Expression of secreted and membrane-bound mucins in the airways of piglets experimentally infected with Mycoplasma hyopneumoniae     

Kim CH  Oh Y  Han K  Seo HW  Kim D  Park C  Kang I  Chae C 《Veterinary journal (London, England : 1997)》2012,192(1):120-122

Immunohistochemistry was used to demonstrate secreted mucins MUC2, MUC5AC and MUC5B and membrane-bound mucin MUC4 in the pulmonary bronchioles of piglets experimentally infected with Mycoplasma hyopneumoniae. Conventional status, Landrace-Duroc cross-bred piglets, 13 days of age, were randomised to two groups. One group (n=20) was infected by the intra-tracheal route with the SNU98703 strain of M. hyopneumoniae, and a group of 12 animals acted as uninfected controls. Five infected and three uninfected piglets were euthanased on the day of infection and at 7, 21, and 35 days post-inoculation (PI). Membrane-bound MUC4 and secreted MUC5AC were the predominant mucins produced in the bronchioles of the piglets in response to M. hyopneumoniae infection, but by day 35 PI, all labelled mucins had returned to pre-infection levels, contemporaneous with reduced pulmonary lesion scores. The increased mucin production may result from direct stimulation of the epithelium by mycoplasmal infection, or may arise indirectly following M. hyopneumoniae-induced ciliostasis.  相似文献   

Mice immune responses to Brucella abortus heat shock proteins. Use of baculovirus recombinant-expressing whole insect cells,purified Brucella abortus recombinant proteins,and a vaccinia virus recombinant as immunogens     

Bae JE  Schurig GG  Toth TE 《Veterinary microbiology》2002,88(2):189-202

Brucella abortus resists the microbicidal mechanisms of macrophages, and the expression of its heat shock proteins (HSPs) such as GroEL, GroES and HtrA may play a role in this resistance. Bacterial HSPs can be very immunogenic, inducing protective immunity in various types of bacterial infections. However, the significance of immune responses directed against B. abortus HSPs in the protection against brucellosis is currently unresolved. To elucidate the role of these proteins in protection against Brucella challenge, individual, divalent or trivalent baculovirus (BV) recombinants of B. abortus GroEL, GroES and/or HtrA were injected into BALB/c mice either as protein-expressing whole cells or as purified proteins. The preparations were given to mice in combination with Freund's or Ribi adjuvant, respectively. In addition, some mice were primed with a vaccinia virus-GroEL recombinant, followed by inoculation with purified GroEL-Ribi adjuvant combination. Antibodies were observed against B. abortus GroEL and HtrA, but not against GroES. Cellular immune response was demonstrated by observing significant IFN-gamma release by lymphocytes of mice immunized with the purified HtrA-Ribi adjuvant combination. However, none of the mice inoculated with individual, divalent or trivalent HSP-expressing cells combined with complete Freund's adjuvant or inoculated with purified B. abortus HSPs combined with Ribi adjuvant, were protected against challenge with B. abortus virulent strain 2308. Priming with vaccinia virus-GroEL recombinant and boosting with GroEL-Ribi combination did not induce protective immunity. Based on the results obtained, we suggest that although humoral and cell-mediated immune responses are induced, but protective immune response is not induced by B. abortus HSPs.  相似文献   

Distribution and characterization of class 1 integrons in Salmonella enterica serotype Gallinarum biotype Gallinarum     

Kwon HJ  Kim TE  Cho SH  Seol JG  Kim BJ  Hyun JW  Park KY  Kim SJ  Yoo HS 《Veterinary microbiology》2002,89(4):303-309

Fowl typhoid caused by Salmonella enterica subsp. enterica serotype Gallinarum biotype Gallinarum is the most important chicken disease in Korea. Due to appearance of new or multiple antibiotics resistances in the recently isolated strains, it was difficult to control the disease using antibiotics in our country. Therefore, the prevalence and genetic contents of class 1 integrons in biotype Gallinarum isolated between 1992 and 2001 were investigated by PCR and direct sequencing, respectively. Out of 90 strains, 35 (39%) carried class 1 integrons. The 1.0, 1.6 and 2.0kbp amplicons were amplified in 32 strains (36%), 2 strains (2%) and 1 strain (1%), respectively. The 1.0, 1.6 and 2.0 kbp amplicons contained one (aadA1a), two (aadB-aadA1b) and three cassettes (dhfrXII-orfF-aadA2), respectively, providing resistances against aminoglycosides (aadA1a, aadA1b, aadB, and aadA2) and trimethoprim (dhfrXII). The integron-carrying strains of biotype Gallinarum appeared in 1996 and acquired additional cassettes in 2000. Although the resistances to ampicillin, tetracycline and chloramphenicol are unrelated to class 1 integrons, relatively high prevalence of integron in biotype Gallinarum may be a dormant threat to the chemotherapy of the disease in the near future because of potency to acquire additional antibiotics resistances.  相似文献   

Effects of soil scraping on the reclamation of tsunami-damaged paddy soil     

Dong-Jin Kang  Akira Endo  Young-Jin Seo 《Journal of Crop Science and Biotechnology》2013,16(3):219-223

We investigated the possibility of tsunami-damaged paddy soil remediation in Japan using a scraping method. We collected undisturbed soil blocks to maintain the soil structure from paddy damaged by salt from the tsunami. Scraping treatments to remove the top 2 cm of the soil surface were conducted once, twice, and thrice after drying naturally in a greenhouse environment, as well as a noscraping control. Electrical conductivity (EC) of the soil dramatically decreased to < 1.36 dS m^?1 in the residual soils after scraping. The EC values of the residual soils were significantly lower after being scraped twice or thrice compared with a single scraping. Similarly, the mean NaCl removal rates were significantly higher with two (91%) or three scrapings (97.2%) compared with a single scraping (73.3%). The growth of rice plants was almost normal in the residual soils based on a visual score (approximately 3 on average) using a standard evaluation system at 4 weeks after transplantation. There were no significant differences in the grain yields from the residual soils with different scraping frequencies, and the EC values of all the residual soils were < 1.36 dS m^?1. This study clearly indicates that there were reductions in the EC and good plant growth performance in the residual soils after scraping, and there was also a high rate of NaCl removal. Therefore, scraping may be a useful method for the remediation of salt-damaged paddy soils when irrigation or culvert systems are affected severely by tsunamis and earthquakes.  相似文献   

Modulatory effects of functional rice cultivars giant embryo and Aranghyangchal on the body weight and lipid metabolism in mice fed with a high fat diet   总被引：1，自引：0，他引：1  

Mi Young Kang  Jung Eun Moon  Sang Chul Lee 《Journal of Crop Science and Biotechnology》2013,16(2):167-171

The effects of the functional rice cultivars giant embryo and Aranghyangchal on the body weight and lipid metabolism in male C57BL/6N mice under high fat diet conditions were investigated. The animals were randomly placed into four dietary groups: normal control (NC group), high fat (HF group), and high fat supplemented with giant embryo (HF-GE group) or Aranghyangchal (HF-AR group). After 8weeks, a marked increase in the body weight and plasma triglyceride and total cholesterol concentrations were found in the HF mice relative to the control group. However, the addition of the functional rice samples in the high fat diet reduced the body weight gain and significantly decreased the lipid levels through inhibition of lipogenesis and regulation of adipokine production. The results demonstrate that the functional rice grains giant embryo and Aranghyangchal may both be useful as biomaterials in the development of functional food with therapeutic potential against high fat diet-induced hyperlipidemia.  相似文献   

Comparative analysis of physicochemicals and antioxidative properties in new red rice (Oryza sativa L. cv. Gunganghongmi)     

Woo Duck Seo  Jun Young Kim  You-Chun Song  Jun-Hyun Cho  Ki Chang Jang  Sang-Ik Han  Ji-Eun Ra  Seong-Hwan Oh  Hyeon-Jung Kang  Byung-Joo Kim  Nam-In Baek  Rak-Hun Jeong  Min Hee Nam 《Journal of Crop Science and Biotechnology》2013,16(1):63-68

The main objectives of this study were to investigate physicochemicals and antioxidant activities of new red rice (Oryza sativa cv. Gunganghongmi (GH)) by comparing normal brown (Nampyeongbyeo, NB) and reported red rice (Jukjinjubyeo, JB) in Korea. The nutritional constituents, including protein, oil, sugar, fatty acid, GABA, and γ-oryzanol were not significantly different between normal brown and colored rice. However, the ethanol extract of GH showed the highest phenolic content (24.7 ± 1.3 mg g^?1). The ethanol extracts of GH showed higher scavenging activities against DPPH (0.2 mg mL^?1 = 62.1 ± 2.5%) and ABTS (0.2 mg mL^?1 = 63.2 ± 3.5%) radicals. Moreover, GH more inhibited LPS-induced nitric oxide (NO) production (13.2 ± 1.4 μM) than JB (18.3 ± 2.3 μM) and NB (22.1 ± 1.4 μM) at the same concentration (0.2 mg mL^?1) without cytotoxicity. These results suggest that new red rice (GH) would be considered to be new functional rice due to its anti-oxidative effect and high nutrition.  相似文献   

Colorimetric detection of transition metal ions with azopyridine-based probing molecule in aqueous solution and in PMMA film     

Daigeun Kim  Ara Jo  Bum-Kyoung Seo  Kune-Woo Lee  Won Ho Park  Taek Seung Lee 《Fibers and Polymers》2013,14(12):1993-1998

We report on an azopyridine derivative for probing transition metal ions and fabrication of its films. The probe, AP, showed a sensitive absorption change toward transition metal ions, especially cobalt ion, even at the concentration of ppm range, accompanied by yellow-to-red color transition with noticeable isosbestic point. In addition, the AP-containing PMMA film with high transparency can be fabricated by spin-casting without any aggregation of AP. The film with PMMA matrix shows good sensitivity toward cobalt ion similar to the case in the solution with a feature of metallochromic transition.  相似文献   

Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits     

Tae-Hyun Kim  Young-Woo Park  Jae-Sang Ahn  Jeong-Taek Ahn  Se-Eun Kim  Man-Bok Jeong  Min-Su Seo  Kyung-Sun Kang  Kang-Moon Seo 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(1):61-67

This study was performed to evaluate the effects of conditioned media (CM) from human amniotic epithelial cells (HAECs) on the corneal wound healing process. Eighteen rabbits (36 eyes) were used and randomly assigned to three groups according treatment: CM from HAECs (group 1), vehicle alone (group 2), and saline (group 3). Corneal alkali injuries were induced with 1 N sodium hydroxide. Each reagent used for treatment evaluation was injected into the dorsal bulbar subconjunctiva and the area of the corneal epithelial defect was measured every other day. Two animals from each group were euthanized at a time on days 3, 7, and 15, and the cornea was removed for histological examination. The sum of the epithelial defect areas measured on day 0 to day 6 as well as day 0 to day 14 in group 1 was significantly smaller than those of other groups. Histological examination revealed that the group 1 corneas had less inflammatory cell infiltration and showed more intact epithelial features compared to the other groups. These results suggest that CM from HAECs promote corneal wound healing in rabbits.  相似文献   

Abundance of biting midge species (Diptera: Ceratopogonidae,Culicoides spp.) on cattle farms in Korea     

Jae-Ku Oem  Joon-Yee Chung  Mee-Soon Kwon  Toh-Kyung Kim  Tae-Uk Lee  You-Chan Bae 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(1):91-94

Culicoides biting midges were collected on three cattle farms weekly using light traps overnight from May to October between 2010 and 2011 in the southern part of Korea. The seasonal and geographical abundance of Culicodes spp. were measured. A total of 16,538 biting midges were collected from 2010 to 2011, including seven species of Culicoides, four of which represented 98.42% of the collected specimens. These four species were Culicodes (C.) punctatus (n = 14,413), C. arakawae (n = 1,120), C. oxystoma (n = 427), and C. maculatus (n = 318). C. punctatus was the predominant species (87.15%).  相似文献