Citral and linalool chemotypes of <Emphasis Type="Italic">Lippia alba</Emphasis> essential oil as anesthetics for fish: a detailed physiological analysis of side effects during anesthetic recovery in silver catfish (<Emphasis Type="Italic">Rhamdia quelen</Emphasis>)     

Carine de Freitas Souza  Matheus Dellaméa Baldissera  Adriane Erbice Bianchini  Elisia Gomes da Silva  Rosa Helena Veraz Mourão  Lenise Vargas Flores da Silva  Denise Schmidt  Berta Maria Heinzmann  Bernardo Baldisserotto 《Fish physiology and biochemistry》2018,44(1):21-34

The viability using Lippia alba essential oil as an anesthetic for fish was studied, particularly with respect to physiological effects during recovery. Anesthesia of silver catfish (Rhamdia quelen) using 100 and 300 μL L^?1 of two different chemotypes of L. alba essential oil (citral EO-C and linalool EO-L) prevented the increase of plasma cortisol levels caused by handling, but did not avoid alterations in energetic metabolism. Silver catfish did not have increased the levels of thiobarbituric acid reactive species in the kidney and liver during recovery after anesthesia with either EO, avoiding lipid damage. On the other hand, fish anesthetized with EO-C showed higher protein carbonylation levels, superoxide dismutase, catalase, and glutathione S-transferase activities and non-protein thiol group levels in both tissues compared to controls. Our results suggest that both oils show antioxidant capacity, but anesthesia with EO-L does not cause damage to lipids or proteins, only temporary changes, typical of physiological adjustments during recovery from anesthesia. Therefore, EO-L is an effective anesthetic for silver catfish with fewer side effects than EO-C.  相似文献   

Loss of genetic variability in the captive stocks of tambaqui,Colossoma macropomum (Cuvier, 1818), at breeding centres in Brazil,and their divergence from wild populations          下载免费PDF全文

Jonas da Paz Aguiar  Paola Fabiana Fazzi Gomes  Igor Guerreiro Hamoy  Sidney Emanuel Batista dos Santos  Horacio Schneider  Iracilda Sampaio 《Aquaculture Research》2018,49(5):1914-1925

The loss of variability in farmed populations and the risks of interactions with wild populations support the need for the genetic monitoring of species farmed throughout the world. In Brazil, the tambaqui is the most widely farmed native fish species. Despite this, there are no data on the pedigree of the farmed stocks, and the potential for interactions with wild populations in the Amazon basin has raised concerns with regard to the genetic variability of these stocks. The present study analysed sequences of the mitochondrial Control Region and 12 microsatellites to characterize the genetic variability of seven historically important commercial tambaqui breeding centres located in four different regions of Brazil, and compared these sequences with those obtained from individuals collected from a wild population. High levels of genetic diversity were found in the wild population, whereas genetic diversity was reduced in both markers in most captive populations, except for the broodstock located near the Amazon River. High F_ST and D_EST indices were recorded between the wild population and most of the captive stocks analysed. The drastic reduction in genetic diversity found in most captive stocks and the difference between these stocks and the wild population may have been the result of the small size of the founding populations and the absence of breeding management. The renewal of the broodstocks and the application of breeding management techniques are recommended. In the Amazon region, in addition, the use of broodstocks that are genetically very different from local wild populations should be avoided.  相似文献   

Soybean seed coat chitinase as a defense protein against the stored product pest Callosobruchus maculatus          下载免费PDF全文

Nadia CM Silva  Jamile G Conceição  Kayan Eudorico Ventury  Leonardo FR De Sá  Eduardo AG Oliveira  Izabela S Santos  Valdirene M Gomes  Monique N Costa  Andre TS Ferreira  Jonas Perales  Jose Xavier‐Filho  Kátia VS Fernandes  Antonia EA Oliveira 《Pest management science》2018,74(6):1449-1456

  相似文献   

Bioefficacy of dietary chromium picolinate and chromium yeast on growth performance and blood biochemical in Red Tilapia,Oreochromis niloticus (Linnaeus)          下载免费PDF全文

Rakhmawati Rakhmawati  Muhammad Agus Suprayudi  Mia Setiawati  Widanarni Widanarni  Muhammad Zairin Junior  Dedi Jusadi 《Aquaculture Research》2018,49(2):839-846

  相似文献   

Impact of foliar nickel application on urease activity,antioxidant metabolism and control of powdery mildew (Microsphaera diffusa) in soybean plants          下载免费PDF全文

J. P. Q. Barcelos  H. P. G. Reis  C. V. Godoy  P. L. Gratão  E. Furlani Junior  F. F. Putti  M. Campos  A. R. Reis 《Plant pathology》2018,67(7):1502-1513

Nickel (Ni) is a cofactor for urease, an enzyme that breaks down urea into ammonia and carbon dioxide. This study aimed to evaluate the physiological impact of Ni on urea, antioxidant metabolism and powdery mildew severity in soybean plants. Seven levels of Ni (0, 10, 20, 40, 60, 80 and 100 g ha^?1) alone or combined with the fungicides fluxapyroxad and pyraclostrobin were applied to soybean plants. The total Ni concentration ranged from 3.8 to 38.0 mg kg^?1 in leaves and 3.0 to 18.0 mg kg^?1 in seeds. A strong correlation was observed between Ni concentration in the leaves and seeds, indicating translocation of Ni from leaves to seeds. Application of Ni above 60 g ha^?1 increased lipid peroxidation in the leaf tissues, indicative of oxidative stress. Application of 40 g ha^?1 Ni combined with 300 mL ha^?1 of fungicide reduced powdery mildew severity by up to 99%. Superoxide dismutase, catalase, peroxidase and urease enzyme activity were greatest under these conditions. Urea concentration decreased in response to Ni application. Urease activity in soybean leaves showed a negative correlation with powdery mildew severity. The leaf Ni concentration showed a positive correlation with the urease and a negative correlation with powdery mildew severity. The results of this study suggest that urease is a key enzyme regulated by Ni and has a role in host defence against powdery mildew by stimulating antioxidant metabolism in soybean plants.  相似文献   

Seed longevity and seedling emergence rate of Urochloa decumbens as influenced by sowing depth in a Cerrado soil          下载免费PDF全文

A. B. Dantas‐Junior  C. Musso  H. S. Miranda 《Grass and Forage Science》2018,73(3):811-814

Urochloa decumbens (Signal grass) is an African grass species that is widely used in Brazil for pasture formation, and it has a high potential for invading natural areas. This study evaluated the longevity of U. decumbens seeds in the soil seed bank and the emergence rates of seedlings from different depths. In the greenhouse (natural light; ~25°C), seeds were sown at depths of 0, 1, 2 or 3 cm, in trays filled with sterile soil, to assess seedling emergence. Seeds sowed on the soil surface began to germinate 3 days after sowing. Seeds sowed at depths of 2 and 3 cm germinated 5 days after sowing. No seedlings emerged after 15 days. The seedling emergence rate was 74 ± 7% with no significant difference between sowing depths. In the field, seed longevity was evaluated by burying 60 replicates of 100 seeds at a soil depth of 2–3 cm, in a Cerrado area in the IBGE Ecological Reserve, Brasília. From September 2016 to April 2017, five samples were retrieved monthly to assess seed viability. In September, the viability was 68%, declining to 3% in October; by February, viable seeds were no longer recorded. As U. decumbens flowers in several episodes throughout the year, a soil survey may result in a misleading interpretation of the formation of a permanent soil seed bank.  相似文献   

The effects of osmolality on sperm quality in <Emphasis Type="Italic">Jenynsia multidentata</Emphasis> (Cyprinodontiformes: Anablepidae)     

Janaína?Camacho?da?Silva  Antonio?Sergio?Varela Junior  J?sie?Shwartz?Caldas  Clarissa?da?Silva Freitas  Joziel?Gon?alves?Botelho  Elton?Pinto?Colares  Carine?Dahl?CorciniEmail authorView author&#;s OrcID profile 《Fish physiology and biochemistry》2016,42(1):93-102

Sperm quality tests on fish are classically used for evaluating cryopreservation procedures, and they are also promising to assess aquatic toxicity and biomarkers of xenobiotic effects on reproduction. Osmotic shock from the storage medium is one of the main factors affecting sperm quality during evaluation. Thus, the objective of this study was to evaluate the effects of different osmolalities (240–460 mOsm/kg) for at least 4 days on the sperm quality parameters of the viviparous fish Jenynsia multidentata. The level of significance was (P < 0.05). The plasma osmolality of J. multidentata is 326 ± 3.9 mOsm/kg. The motility of fresh semen was higher in osmolalities of 280 and 300 mOsm/kg but did not differ between osmolalities from 240 to 320 mOsm/kg. Above 380 mOsm/kg, the motility observed was 0 %. Over the time period studied motility increased with increasing osmolality, and the most constant and long-lasting rates were between 300 and 320 mOsm/kg. On the 4th day of evaluation, higher membrane integrity rates were observed between 280 and 360 mOsm/kg, higher mitochondrial membrane potential was observed between 300 and 460 mOsm/kg, and higher DNA integrity rates were observed between 260 and 380 mOsm/kg. Moreover, osmolalities ≥460 and ≤240 resulted in the lowest motility and DNA integrity levels. Over 4 days, the plasma membrane integrity was significantly lower at ≤260 and ≥400 mOsm/kg, and the mitochondrial membrane potential was significantly lower only in osmolalities ≤240 mOsm/kg. Therefore, we conclude that for sperm quality preservation in J. multidentata, an osmolality of 300–320 mOsm/kg of the most suitable diluent is necessary. Furthermore, we conclude that the storage of sperm in a hyposmotic (<260 mOsm/kg) or hyperosmotic (>400 mOsm/kg) solution affects not only motility but also other sperm quality parameters.  相似文献   

Sodium fluorescein as an internal tracer on the location of bovine urine patches in pastures          下载免费PDF全文

M. R. Gusmão  T. C. Alves  A. P. Lemes  G. M. Bettiol  A. de F. Pedroso  W. Barioni Junior  P. P. A. Oliveira  C. R. Grego 《Grass and Forage Science》2016,71(2):305-314

Sodium fluorescein (SF) was evaluated and validated as an internal marker in cattle for the location of urine patches in pastures. Three trials were carried out aiming at the following: evaluating the effect of dilution and volume of application on SF fluorescence on the environment; testing dosages and duration of excretion of SF administered orally to cattle; and validating the methodology for SF use on the location of cattle urine patches in pastures. The marker was tested in beef cattle kept on pastures under three grazing systems: degraded pasture under extensive management (De), intensively managed dryland pasture with high stocking rate (Id) and intensively managed irrigated pasture with high stocking rate (Ii). Besides the localization of urination sites, the number of urinations/animal/d and the area covered by urine were also determined. The residue of SF remained fluorescent in the pasture foliage up to 34 h after urination events, allowing the location of urine patches by a black‐light flashlight. There was no difference between grazing systems in the number of urine patches/animal/d during the rainy and dry seasons. The average number of urine patches was approximately 11/animal/d. As expected, the estimated volume and area covered by urine varied according to the stocking density. The chosen SF dose (50 mg kg^?1 LW) did not adversely affect the animals when administered once daily during 2 d. However, the same SF dose administered during four consecutive days caused urinary disorder in the animal. The distribution of urine patches was spatially dependent on specific characteristics of the paddock.  相似文献   

Binding of imidacloprid,thiamethoxam and N‐desmethylthiamethoxam to nicotinic receptors of Myzus persicae: pharmacological profiling using neonicotinoids,natural agonists and antagonists          下载免费PDF全文

Hartmut Kayser  Katrin Lehmann  Marilyne Gomes  Wolfgang Schleicher  Karin Dotzauer  Margarethe Moron  Peter Maienfisch 《Pest management science》2016,72(11):2166-2175

  相似文献   

Methods of inoculation and evaluation of Erwinia psidii in eucalypt          下载免费PDF全文

H. G. M. Ferraz  G. A. Demuner  L. M. S. Guimarães  D. A. A. Arriel  A. C. da Silva  N. B. Junior  R. G. Mafia  A. C. Alfenas 《Forest Pathology》2016,46(3):240-247

The dieback and wilting caused by Erwinia psidii are emerging eucalypt diseases that have been observed since 2014 in the south and central‐south regions of Brazil. Field observations have shown variability in disease severity resistance among Eucalyptus spp. clones and species. It is hypothesized that this variability is due to genetic resistance. To confirm this hypothesis, inoculations in genetically distinct eucalypt plants are necessary. However, lack of an inoculation method and disease assessment makes difficult to select resistant genotypes for use in commercial plantations or genetic breeding programmes. Three inoculation methods were tested on eight clones of Eucalyptus spp. Among them, inoculum deposition with bacteria‐impregnated toothpick on the axillary buds was the simplest and most effective, capable to reproduce the disease symptoms observed under conditions of natural infection. We also developed a rating scale for disease assessment. Among eight clones tested, only Clone 1 (Eucalyptus saligna) and Clone 2 (Eucalyptus urophylla) were resistant.  相似文献