Larval rearing of African carp,Labeo parvus Boulenger, 1902 (Pisces: Cyprinidae), using live food and artificial diet under controlled conditions     

Elie Montchowui  Philippe Lalèyè  Emile N'tcha  Jean‐Claude Philippart  Pascal Poncin 《Aquaculture Research》2012,43(8):1243-1250

This study aimed at (1) evaluating the efficacy of live food organisms (Artemia and natural zooplankton) and an artificial diet in the larval rearing of African carp Labeo parvus and (2) determining appropriate rearing conditions. After yolk sac resorption, the larvae were separated into five different feeding trials with two replicates. At the end of the larval rearing period (28 days post‐hatching), the highest (94.6%) and the lowest (53.7%) cumulative survival rates were found when larvae were fed with natural zooplankton for 7 days followed by Nippai food for 21 days, and when larvae were fed from the beginning of exogenous feeding with Nippai food only respectively. The significant highest body weight (351.6 mg), total length (34.4 mm) and specific growth rates (15.5%day^?1) were recorded when the larvae were fed with Artemia nauplii for 14 days followed by Nippai food for 14 days. The lowest growth performance (body weight and specific growth rates) were obtained when larvae were fed exclusively Nippai food. These results indicate that L. parvus can be successfully cultured in indoor nursery systems from hatching to the early juvenile stage.  相似文献   

Enhancement of Stress Resistance of the Guppy Poecilia reticulata through Feeding with Vitamin C Supplement   总被引：2，自引：0，他引：2  

Lian Chuan  Lim Philippe  Dhert Wei Yen  Chew Veerle  Dermaux Hans  Nelis Patrick  Sorgeloos 《Journal of the World Aquaculture Society》2002,33(1):32-40

This study investigated the use of vitamin C supplement in formulated diets and live Artemia juveniles to enhance the stress resistance of the guppy Poecilia reticulata . To evaluate the stress resistance, fish were subjected to osmotic shock in pre-aerated water containing 35 ppt sodium chloride. Ascorbyl acid-polyphosphate and ascorbyl palmitate were used as vitamin C sources for formulated diets and live Artemia juveniles, respectively. Results showed that guppies fed moist formulated diets supplemented with ascorbic acid at 1,000 mg/kg or 2,000 mg/kg diet displayed significantly higher levels of stress resistance than fish fed control formulated diet for 13 d. The stress resistance of fish fed a lower dose (200 mg/kg diet), however, did not differ significantly from that of the control. On the other hand, the stress resistance of guppies fed Artemia juveniles bio-encapsulated with 10% or 20% ascorbyl palmitate did not show significant difference from that of fish fed control Artemia after 20 d. The stress resistance of these three groups of Artemia- fed fish, however, was significantly higher than that of fish fed the control formulated diet. Biochemical analyses showed that raising the ascorbic acid level in feeds resulted in a concomitant increase in the incorporated ascorbic acid level in the whole-body tissue of the guppy. The increased stress resistance of the guppy fed a vitamin C supplemented diet has also been demonstrated in four commercial farms. The potential application of the improved stress resistance in the ornamental fish industry was discussed.  相似文献   

Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of Gambierdiscus polynesiensis     

Hlne Taiana Darius  Taina Revel  Jrme Viallon  Manoëlla Sibat  Philippe Cruchet  Sbastien Longo  Donnie Ransom Hardison  William C. Holland  Patricia A. Tester  R. Wayne Litaker  Jennifer R. McCall  Philipp Hess  Mireille Chinain 《Marine drugs》2022,20(6)

Gambierdiscus and Fukuyoa dinoflagellates produce a suite of secondary metabolites, including ciguatoxins (CTXs), which bioaccumulate and are further biotransformed in fish and marine invertebrates, causing ciguatera poisoning when consumed by humans. This study is the first to compare the performance of the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantitative estimation of CTX contents in 30 samples, obtained from four French Polynesian strains of Gambierdiscus polynesiensis. fRBA was applied to Gambierdiscus matrix for the first time, and several parameters of the fRBA protocol were refined. Following liquid/liquid partitioning to separate CTXs from other algal compounds, the variability of CTX contents was estimated using these three methods in three independent experiments. All three assays were significantly correlated with each other, with the highest correlation coefficient (r² = 0.841) found between fRBA and LC-MS/MS. The CBA-N2a was more sensitive than LC-MS/MS and fRBA, with all assays showing good repeatability. The combined use of fRBA and/or CBA-N2a for screening purposes and LC-MS/MS for confirmation purposes allows for efficient CTX evaluation in Gambierdiscus. These findings, which support future collaborative studies for the inter-laboratory validation of CTX detection methods, will help improve ciguatera risk assessment and management.  相似文献   

Quantification of Common Wheat Adulteration of Durum Wheat Pasta Using Real‐Time Quantitative Polymerase Chain Reaction (PCR)     

Rmi Alary  Arnaud Serin  Marie‐Pierre Duviau  Philippe Jourdrier  Marie‐Franoise Gautier 《Cereal Chemistry》2002,79(4):553-558

Common wheat adulteration of durum wheat pasta was quantified using real‐time duplex polymerase chain reaction (PCR). The total DNA content of pasta was determined by amplifying part of a wheat gene encoding a lipid transfer protein, and common wheat DNA was quantified by amplifying part of the puroindoline‐b gene. Under the conditions defined by this study, for pasta with a theoretical adulteration of 3%, the experimentally determined mean value was 2.6–3.4%, depending on drying temperature. Pure durum wheat pastas were distinguished from adulterated pastas without ambiguity. This study demonstrates the feasibility of using real‐time duplex PCR to quantify common wheat adulteration of pasta dried at high temperature, quantification that was impossible with the French official peroxidase‐marker method.  相似文献   

Hand-feel soil texture observations to evaluate the accuracy of digital soil maps for local prediction of soil particle size distribution: A case study in Central France     

Anne C. RICHER-de-FORGES  Dominique ARROUAYS  Laura POGGIO  Songchao CHEN  Marine LACOSTE  Budiman MINASNY  Zamir LIBOHOVA  Pierre ROUDIER  Vera L. MULDER  Herv&#; N&#;D&#;LEC  Guillaume MARTELET  Blandine LEMERCIER  Philippe LAGACHERIE  Hocine BOURENNANE 《土壤圈》2023,33(5):731-743

Digital maps of soil properties are now widely available. End-users now can access several digital soil mapping (DSM) products of soil properties, produced using different models, calibration/training data, and covariates at various spatial scales from global to local. Therefore, there is an urgent need to provide easy-to-understand tools to communicate map uncertainty and help end-users assess the reliability of DSM products for use at local scales. In this study, we used a large amount of hand-feel soil texture (HFST) data to assess the performance of various published DSM products on the prediction of soil particle size distribution in Central France. We tested four DSM products for soil texture prediction developed at various scales (global, continental, national, and regional) by comparing their predictions with approximately 3 200 HFST observations realized on a 1:50 000 soil survey conducted after release of these DSM products. We used both visual comparisons and quantitative indicators to match the DSM predictions and HFST observations. The comparison between the low-cost HFST observations and DSM predictions clearly showed the applicability of various DSM products, with the prediction accuracy increasing from global to regional predictions. This simple evaluation can determine which products can be used at the local scale and if more accurate DSM products are required.  相似文献