Protection against neurodegenerative diseases of Iris pseudopumila extracts and their constituents     

Filomena Conforti  Daniela Rigano  Federica Menichini  Monica Rosa Loizzo  Felice Senatore 《Fitoterapia》2009

The present study describes for the first time the in vitro properties of Iris pseudopumila flowers and rhizomes extracts and their constituents. The methanolic extract of rhizomes showed significant anti-inflammatory activity through inhibition of NO production in the murine monocytic macrophage cell line RAW 264.7. Among the isolated compounds, those which most effectively inhibited LPS-induced NO production were irisolidone and 7-methyl-tectorigenin-4′-O-[β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside], with IC₅₀ values of 23.6 μM and 29.4 μM respectively. Isoorientin and isovitexin exhibited the most promising activity against AChE with IC₅₀ of 26.8 μM and 36.4 μM, respectively. The same compounds exhibited also the higher activity against BChE.  相似文献   

Effect of Multiple Stressors on Water Quality and Macroinvertebrate Assemblages in an Effluent-Dominated Stream     

Canobbio  Sergio  Mezzanotte  Valeria  Sanfilippo  Umberto  Benvenuto  Federica 《Water, air, and soil pollution》2009,203(1-4):359-368

Water, Air, & Soil Pollution - Mobilization of arsenic (As) in the subsurface environment can result in elevated concentrations of As in groundwater and potential human exposure and adverse...  相似文献   

Segmental tracheal dysplasia in a mixed breed dog     

Mawby DI  Krahwinkel DJ  Donnell RL  Morandi F 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(10):1003-1006

A 6-month-old, spayed female, mixed breed dog was referred for respiratory difficulty. Radiographic and bronchoscopic evaluations showed tracheal narrowing from the thoracic inlet to its bifurcation. Under anesthesia, the trachea ruptured and the dog died. Microscopic evaluation revealed underlying segmental tracheal and bronchial cartilaginous malformation (dysplasia).  相似文献   

Application of multielement stable isotope ratio analysis to the characterization of French, italian, and spanish cheeses   总被引：6，自引：0，他引：6  

Camin F  Wietzerbin K  Cortes AB  Haberhauer G  Lees M  Versini G 《Journal of agricultural and food chemistry》2004,52(21):6592-6601

The stable isotope ratios (delta13C, delta15N, and delta34S of casein and delta13C and delta18O of glycerol) measured by IRMS of French, Italian, and Spanish cheeses are presented and discussed. Variability factors such as animal-feeding regimen, geographical origin, and climatic and seasonal conditions were studied to check the possibilities of cheese characterization offered by each isotopic parameter. Delta13C values of both casein and glycerol appeared to be strongly correlated to the amount of maize in the animal diet. Delta15N and delta34S of casein proved to be mostly influenced by the geoclimatic conditions of the area (aridity, closeness to the sea, altitude). Delta18O of glycerol was more dependent on the geographical origin of the cheeses and on climatic/seasonal parameters. By applying a multivariate stepwise canonical discriminant analysis, good discrimination possibilities for the different European cheeses were obtained, confirmed by the classification analysis, when >90% of the samples were correctly reclassified.  相似文献   

Resveratrol reduces the invasive growth and promotes the acquisition of a long-lasting differentiated phenotype in human glioblastoma cells     

Castino R  Pucer A  Veneroni R  Morani F  Peracchio C  Lah TT  Isidoro C 《Journal of agricultural and food chemistry》2011,59(8):4264-4272

Malignant glioblastoma represents a challenge in the chemotherapy of brain tumors, because of its aggressive behavior characterized by chemoresistance, infiltrative diffusion, and high rate of recurrence and death. In this study, we used cultured human U87MG cells and primary human glioblastoma cultures to test the anticancer properties of resveratrol (RV), a phytoalexin abundantly present in a variety of dietary products. In U87MG cells, 100 μM RV elicited cell growth arrest by 48 h and bax-mediated cell toxicity by 96 h and greatly limited cell migration and invasion through matrigel. Both in U87MG cells and in primary glioblastoma cultures, the chronic administration of RV (100 μM for up to 96 h) decreased the expression of nestin (a brain (cancer) stem cells marker) but increased that of glial acidic fibrillary protein (a mature glial cell marker) and of βIII-tubulin (a neuronal differentiation marker). Chronic treatment with RV increased the proportion of cells positive for senescence-associated β-galactosidase activity. This is the first report showing the ability of RV to induce glial-like and neuronal-like differentiation in glioblastoma cells. The beneficial effects of chronic RV supplementation lasted up to 96 h after its withdrawal from the culture medium. The present findings support the introduction of pulsed administration of this food-derived molecule in the chemotherapy regimen of astrocytomas.  相似文献   

Chromatographic methods for metabolite profiling of virus- and phytoplasma-infected plants of Echinacea purpurea     

Pellati F  Epifano F  Contaldo N  Orlandini G  Cavicchi L  Genovese S  Bertelli D  Benvenuti S  Curini M  Bertaccini A  Bellardi MG 《Journal of agricultural and food chemistry》2011,59(19):10425-10434

This study was focused on the effects of virus and phytoplasma infections on the production of Echinacea purpurea (L.) Moench secondary metabolites, such as caffeic acid derivatives, alkamides, and essential oil. The identification of caffeic acid derivatives and alkamides was carried out by means of high-performance liquid chromatography-diode array detection (HPLC-DAD), HPLC-electrospray ionization-mass spectrometry (ESI-MS), and MS(2). Quantitative analysis of these compounds was carried out using HPLC-DAD. The results indicated that the presence of the two pathogens significantly decreases (P < 0.05) the content of cichoric acid, the main caffeic acid derivative. Regarding the main alkamide, dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide, a significant decrease (P < 0.05) in the content of this secondary metabolite was observed in virus-infected plants in comparison with healthy plants, while in the phytoplasma-infected sample the variation of this secondary metabolite was not appreciable. The % relative area of the E/Z isomers of this alkamide was also found to change in infected samples. The gas chromatography (GC) and GC-MS analysis of E. purpurea essential oil enabled the identification of 30 compounds. The main significant differences (P < 0.05) in the semiquantitative composition were observed for three components: limonene, cis-verbenol, and verbenone. The results indicate that the presence of virus and phytoplasma has an appreciable influence on the content of E. purpurea secondary metabolites, which is an important issue in defining the commercial quality, market value, and therapeutic efficacy of this herbal drug.  相似文献   

Usutu virus in Italy: an emergence or a silent infection?     

Savini G  Monaco F  Terregino C  Di Gennaro A  Bano L  Pinoni C  De Nardi R  Bonilauri P  Pecorari M  Di Gialleonardo L  Bonfanti L  Polci A  Calistri P  Lelli R 《Veterinary microbiology》2011,151(3-4):264-274

A two year study (2008-2009) was carried out to monitor the Usutu virus (USUV) circulation in Italy. Sentinel horses and chickens, wild birds and mosquitoes were sampled and tested for the presence of USUV and USUV antibodies within the WND National Surveillance plan. Seroconversion evidenced in sentinel animals proved that in these two years the virus has circulated in Tuscany, Emilia Romagna, Veneto and Friuli Venezia Giulia regions. In Veneto USUV caused a severe blackbird die-off disease involving at least a thousand birds. Eleven viral strains were detected in organs of 9 blackbirds (52.9%) and two magpies (0.5%) originating from Veneto and Emilia Romagna regions. USUV was also detected in a pool of Culex pipiens caught in Tuscany. According to the alignment of the NS5 partial sequences, no differences between the Italian USUV strains isolated from Veneto, Friuli and Emilia Romagna regions were observed. The Italian North Eastern strain sequences were identical to those of the strain detected in the brain of a human patient and shared a high similarity with the isolates from Vienna and Budapest. Conversely, there were few differences between the Italian strains which circulated in the North Eastern regions and the USUV strain detected in a pool of C. pipiens caught in Tuscany. A high degree of similarity at both nucleotide and amino acid level was also found when the full genome sequence of the Italian North Eastern isolate was compared with that of the strains circulating in Europe. The North Eastern Italian strain sequence exhibited 97% identity to the South African reference strain SAAR-1776. The deduced amino acid sequences of the Italian strain differed by 10 and 11 amino-acids from the Budapest and Vienna strains, respectively, and by 28 from the SAAR-1776 strain. According to this study two strains of USUVs are likely to have circulated in Italy between 2008 and 2009. They have developed strategies of adaptation and evolution to spread into new areas and to become established.  相似文献   

Ultrasonographic Diagnosis of Acute Extrusion of the Lateral Meniscus in a Competing Quarter Horse     

Federica ter Woort  Virginie De Busscher  Christopher B. Riley 《Journal of Equine Veterinary Science》2011,31(2):53-56

A 4-year-old Quarter Horse gelding with a history of acute trauma was presented to our faculty. This report describes the inciting injury, documented in a video, and the ultrasonographic diagnosis of a traumatic extrusion of the right lateral meniscus with an accompanying extra-articular hematoma and distal collateral ligament lesion in a competing Quarter Horse. The ultrasonographic diagnosis of a lateral meniscal injury directly correlated with postmortem findings and this type of lateral meniscal injury has not been previously described in detail.  相似文献   

Clinical pharmacokinetics of a dexmedetomidine–methadone combination in dogs undergoing routine anaesthesia after buccal or intramuscular administration     

Federica Di Cesare  Daniela Gioeni  Giuliano Ravasio  Alberto Pellegrini  Lorena Lucatello  Vittoria Bisutti  Roberto Villa  Petra Cagnardi 《Journal of veterinary pharmacology and therapeutics》2019,42(4):392-400

This study aimed to define the pharmacokinetic profiles of dexmedetomidine and methadone administered simultaneously in dogs by either an oral transmucosal route or intramuscular route and to determine the bioavailability of the oral transmucosal administration relative to the intramuscular one of both drugs, so as the applicability of this administration route in dogs. Twelve client‐owned dogs, scheduled for diagnostic procedures, were treated with a combination of dexmedetomidine hydrochloride (10 μg/kg) and methadone hydrochloride (0.4 mg/kg) through an oral transmucosal route or intramuscularly. Oral transmucosal administration caused ptyalism in most subjects, and intramuscular administration caused transient peripheral vasoconstriction. The results showed reduced and delayed absorption of both dexmedetomidine and methadone when administered through an oral transmucosal route, with median (range) C_max values of 0.82 (0.42–1.49) ng/ml and 13.22 (2.80–52.30) ng/ml, respectively. The relative bioavailability was low: 16.34% (dexmedetomidine) and 15.5% (methadone). Intramuscular administration resulted in a more efficient absorption profile, with AUC and C_max values for both drugs approximately 10 times higher. Dexmedetomidine and methadone administered simultaneously by an oral transmucosal route using injectable formulations were not well absorbed through the oral mucosa. Nevertheless, additional studies on these drugs combination using alternative administration routes are recommended.  相似文献   

Isolation, in vitro culture and characterization of foal umbilical cord stem cells at birth     

Cremonesi F  Violini S  Lange Consiglio A  Ramelli P  Ranzenigo G  Mariani P 《Veterinary research communications》2008,32(Z1):S139-S142

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