Seroprevalence for norovirus genogroups GII and GIV in captive non‐human primates     

Federica Di Profio  Vittorio Sarchese  Irene Melegari  Andrea Palombieri  Ivano Massirio  Sandra Bermudez Sanchez  Klaus Gunther Friedrich  Federico Coccia  Fulvio Marsilio  Vito Martella  Barbara Di Martino 《Zoonoses and public health》2019,66(3):310-315

Noroviruses (NoVs) are a major cause of epidemic gastroenteritis in children and adults. Several pieces of evidence suggest that viruses genetically and antigenically closely related to human NoVs might infect animals, raising public health concerns about potential cross‐species transmission. The natural susceptibility of non‐human primates (NPHs) to human NoV infections has already been reported, but a limited amount of data is currently available. In order to start filling this gap, we screened a total of 86 serum samples of seven different species of NPHs housed at the Zoological Garden (Bioparco) of Rome (Italy), collected between 2001 and 2017, using an enzyme‐linked immunosorbent assay (ELISA) based on virus‐like particles (VLPs) of human GII.4 and GIV.1 NoVs. Antibodies specific for both genotypes were detected with an overall prevalence of 32.6%. In detail, IgG antibodies against GII.4 NoVs were found in 18 Japanese macaques (29.0%, 18/62), a mandrill (10.0%, 1/10), a white‐crowned mangabey (16.6%, 1/6) and in an orangutan (33.3%, 1/3). Twelve macaques (19.3%, 12/62), five mandrills (50.0%, 5/10), two chimpanzees (100%, 2/2) and a white‐crowned mangabey (16.6%, 1/6) showed antibodies for GIV.1 NoVs. The findings of this study confirm the natural susceptibility of captive NHPs to GII NoV infections. In addition, IgG antibodies against GIV.1 were detected, suggesting that NHPs are exposed to GIV NoVs or to antigenically related NoV strains.  相似文献   

An open study on the efficacy of a recombinant Der f 2 (Dermatophagoides farinae) immunotherapy in atopic dogs in Hungary and Switzerland          下载免费PDF全文

Nina Fischer  Noémi Tarpataki  Federica Leidi  Ana Rostaher  Claude Favrot 《Veterinary dermatology》2018,29(4):337-e118

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Stomach wall evaluation using helical hydro-computed tomography     

Terragni R  Vignoli M  Rossi F  Laganga P  Leone VF  Graham JP  Russo M  Saunders JH 《Veterinary radiology & ultrasound》2012,53(4):402-405

In helical hydro-computed tomography (helical hydro-CT), water is used as a neutral luminal contrast medium together with intravenous iodine contrast medium for the diagnosis and staging of human gastric neoplasia. We evaluated the feasibility of helical hydro-CT in 11 healthy animals (nine dogs and two cats). Adequate uniform gastric distension was obtained with 30 ml water/kg body weight. Fourteen client-owned dogs and four cats with suspected or diagnosed gastric neoplasia then underwent helical hydro-CT followed by intravenous contrast medium administration. Focal thickening with moderate contrast enhancement was found in 10 dogs and 3 cats. The extent of the lesion was assessed easily in all these patients. Three dogs and one cat had a normal stomach wall. One dog had multifocal thickening of the antrum but no histopathologic diagnosis was made. Helical hydro-CT, followed by intravenous contrast medium administration, is a simple technique for assessing the stomach wall.  相似文献   

What is your diagnosis? Infection with dimorphic Blastomyces dermatitidis     

Greene JP  Morandi F  Wells JE 《Journal of the American Veterinary Medical Association》2012,240(8):945-946

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Computed tomography-guided fine-needle aspiration and tissue-core biopsy of bone lesions in small animals     

Massimo Vignoli  DR. MED. VET.  SRV    Stefanie Ohlerth  DR. MED. VET.    Federica Rossi  DR. MED. VET.  SRV    Luigi Pozzi  PROF. DR. MED. VET.    Rossella Terragni  DR. MED. VET.    Daniele Corlazzoli  DR. MED. VET.    Barbara Kaser-Hotz  PROF. DR. MED. Vet 《Veterinary radiology & ultrasound》2004,45(2):125-130

In humans, free-hand computed tomography (CT)-guided biopsy is an accurate method to obtain a tissue sample. There are only a few reports of this technique in veterinary medicine. In the present study, 21 dogs and two cats underwent a free-hand CT-guided tissue-core biopsy (17 animals) or fine-needle aspiration (six animals) of a bone lesion. Two out of 17 tissue-core samples were also cultured. All 17 tissue-core biopsy samples were diagnostic (accuracy of 100%). Five out of six aspirates were diagnostic (accuracy of 83.3%). The overall accuracy was 95.7%. In one aspirate, cytologic quality was insufficient containing only blood. No major complications were encountered. Fourteen neoplastic, two infectious and six benign lesions were diagnosed. CT examination after intravenous contrast medium added useful information to avoid large vessels and to biopsy-viable tissue. Free-hand CT-guided tissue-core biopsy and aspiration appears to be a safe and very accurate procedure for use in the diagnosis of bone-associated diseases in small animals.  相似文献   

A study of mutations in the c-kit gene of 32 dogs with mastocytoma.     

Federica Riva  Stefano Brizzola  Damiano Stefanello  Simone Crema  Lauretta Turin 《Journal of veterinary diagnostic investigation》2005,17(4):385-388

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Analysis of mouse, rat, dog, marmoset, and human serum proteins by capillary electrophoresis: comparison with agarose gel electrophoresis     

Crivellente F  Bonato M  Cristofori P 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(1):73-78

BACKGROUND: Serum protein analysis in both humans and experimental animal species has so far been carried out by labor-intensive techniques, such as agarose gel electrophoresis (AGE). OBJECTIVE: The objective of this study was to evaluate capillary electrophoresis (CE) as an alternative technique to AGE for the analysis of serum proteins from healthy animals. METHODS: Blood samples were collected into tubes without anticoagulant from 6 fasted healthy male mice, rats, dogs, marmosets, and humans. Serum proteins were separated by CE using a technique standardized for the analysis of human proteins, and the results (efficiency, resolution, and precision) were compared with those obtained through AGE. RESULTS: Compared with AGE, CE resulted in narrower peaks and more peaks. The efficiency of protein separation by CE was significantly higher for all species, and resolution (R) was significantly higher in samples from dogs. Using rat serum, intraday reproducibility was lower for all protein fractions, and interday reproducibility was lower for most peaks, compared with AGE. CONCLUSIONS: We conclude that CE is a viable alternative to AGE for the determination of protein electrophoresis in a routine veterinary clinical pathology laboratory. The minimal sample requirement (2 microL), complete automation, and quantitative results make CE an especially valuable technique for protein analysis in experimental animal models.  相似文献   

SPINAL SUBARACHNOID CYST IN A CAT     

Massimo  Vignoli  DVM  SRV  Federica  Rossi  DVM  SRV  Giuseppe  Sarli  DVM 《Veterinary radiology & ultrasound》1999,40(2):116-119

A 7-year-old neutered female domestic short hair cat was presented with hind limb ataxia. A subarachnoid cyst in the T10-T11 spinal cord region was identified by myelography as a collection of contrast medium in the subarachnoid space.  相似文献   

The emergence of Schmallenberg virus across Culicoides communities and ecosystems in Europe     

Thomas Balenghien  Nonito Pagès  Maria Goffredo  Simon Carpenter  Denis Augot  Elisabeth Jacquier  Sandra Talavera  Federica Monaco  Jérôme Depaquit  Colette Grillet  Joan Pujols  Giuseppe Satta  Mohamed Kasbari  Marie-Laure Setier-Rio  Francesca Izzo  Cigdem Alkan  Jean-Claude Delécolle  Michela Quaglia  Rémi Charrel  Andrea Polci  Emmanuel Bréard  Valentina Federici  Catherine Cêtre-Sossah  Claire Garros 《Preventive veterinary medicine》2014

Schmallenberg virus (SBV), a novel arboviral pathogen, has emerged and spread across Europe since 2011 inflicting congenital deformities in the offspring of infected adult ruminants. Several species of Culicoides biting midges (Diptera: Ceratopogonidae) have been implicated in the transmission of SBV through studies conducted in northern Europe. In this study Culicoides from SBV outbreak areas of mainland France and Italy (Sardinia) were screened for viral RNA. The role of both C. obsoletus and the Obsoletus complex (C. obsoletus and C. scoticus) in transmission of SBV were confirmed in France and SBV was also discovered in a pool of C. nubeculosus for the first time, implicating this species as a potential vector. While collections in Sardinia were dominated by C. imicola, only relatively small quantities of SBV RNA were detected in pools of this species and conclusive evidence of its potential role in transmission is required.  相似文献   

COMPUTED TOMOGRAPHIC CHARACTERISTICS OF EOSINOPHILIC PULMONARY GRANULOMATOSIS IN FIVE DOGS     

Caroline Fina  Massimo Vignoli  Rossella Terragni  Federica Rossi  Erik Wisner  Jimmy H. Saunders 《Veterinary radiology & ultrasound》2014,55(1):16-22

Canine pulmonary eosinophilic granulomatosis is a rare inflammatory pulmonary disease characterized by formation of eosinophilic granulomas that tend to obliterate the normal pulmonary architecture. The purpose of this retrospective study was to describe the CT characteristics of confirmed idiopathic pulmonary eosinophilic granulomatosis in a group of dogs. Five dogs met inclusion criteria. All patients were young adult dogs of variable breeds. No dog had concurrent occult heartworm disease. Computed tomographic characteristics most commonly included pulmonary masses and nodules of variable size, and lesions were most commonly located in the caudal lung lobes. Four dogs had large pulmonary masses with or without additional nodules and one dog had nodular lesions disseminated throughout the entire lung parenchyma. All large eosinophilic granulomas were smoothly margined, heterogeneous pulmonary masses displaying heterogeneous contrast enhancement. A honeycomb‐like enhancement pattern was observed in all but one mass and consisted of multiple hyperattenuating rims delineating central hypoattenuating areas, suggestive of bronchiectatic lung with peripheral enhancing airway walls and fluid‐filled, necrotic bronchial lumen. One dog had evidence of tracheobronchial lymphadenopathy. Findings indicated that canine eosinophilic pulmonary granulomatosis should be included as a differential diagnosis for dogs with CT characteristics of multiple pulmonary masses and/or nodules in caudal lung lobes, and a honeycomb‐like enhancement pattern in masses after intravenous administration of iodinated contrast medium.  相似文献