Guava decline: effect of root exudates from Meloidogyne enterolobii-parasitized plants on Fusarium solani in vitro and on growth and development of guava seedlings under controlled conditions     

Vicente Martins Gomes  Ricardo Moreira Souza  Silvaldo Felipe da Silveira  Alexandre Macedo Almeida 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(2):393-401

In guava decline, Fusarium solani-immune guava trees become susceptible to extensive root rot caused by this fungus after parasitism by Meloidogyne enterolobii. To understand the mechanisms involved in this disease, root exudates were collected from nematode-inoculated (NI) or uninoculated (UN) guava plants cultivated in sand. After filtration through a Millipore® membrane, NI and UN exudates were used: i) to prepare media to assess their effect on mycelial growth and production of propagules of F. solani isolate UENF/CF 163, and ii) to incubate macro- and microconidia to assess their effect on germination. NI exudates promoted (P?<?0.05) more mycelial growth and production of propagules than UN exudates or water. NI and UN exudates were used to water guava seedlings laid over seed germination paper inside plastic boxes. Half of the seedlings had an agar plug colonized by the fungus positioned in the collar region. Upon watering with NI exudates the fungus caused (P?<?0.05) extensive rotting of the seedlings’ rootlets. NI and UN exudates, either unlyophilized or lyophilized and re-suspended to the original concentration, were used to water guava seedlings grown in sterile sand before being inoculated (or left uninoculated) as described before. Solely upon watering with NI exudates, in its unlyophilized form or after lyophilization, the fungus caused a reduction (P?<?0.05) of shoot and root biomass associated with rotting of roots. These results suggest that M. enterolobii induces chemical changes in the root exudates of guava trees, which are necessary for root invasion causing root rotting by F. solani.  相似文献   

Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum     

R. Narancio  P. Zorrilla  C. Robello  M. Gonzalez  F. Vilaró  C. Pritsch  M. Dalla Rizza 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(4):823-835

Solanum commersonii is a wild species related to the cultivated potato. Some S. commersonii genotypes have been proven to be resistant to the pathogenic bacteria Ralstonia solanacearum, which causes damage in potato and other economically important crops. Here an expression analysis of the response of a resistant S. commersonii genotype against R. solanacearum was performed using microarrays. The aims of this work were to elucidate the molecular processes involved in the interaction, establish the timing of the response, and contribute to identify genes related to the resistance. The response to the treatment was already initiated at 6 h post-inoculation (hpi) and was established at 24 hpi; during this period, a high number of genes was differentially expressed and several candidate genes for the resistance of S. commersonii to R. solanacearum were identified. At an early stage, the photosynthetic process was highly repressed and several genes encoding proteins related to reactive oxygen species (ROS) production were differentially expressed. The induction of ERF and ACC-oxidase genes related to the ethylene pathway and PR1 related to the salicylic acid pathway suggested the induction of both pathways, and back up the previously reported hemibiotrophic nature of the pathogen. Five genes related to plant defence and observed to be differentially expressed at the first two time points were validated by real time PCR. This work gives a glimpse to the molecular processes involved in S. commersonii resistance and identifies the species as a valuable genetic source for potato breeding against bacterial wilt.  相似文献   

Molecular sequence data of Thecaphora frezii affecting peanut crops in Argentina     

Cinthia Conforto  Ignacio Cazón  Franco D. Fernández  Adriana Marinelli  Claudio Oddino  Alejandro M. Rago 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(4):663-666

In Argentina, Thecaphora frezii is the causal agent of peanut smut, causing severe yield losses in peanut growing areas. In this work sequence data of the large ribosomal subunit (28S rDNA) of four T. frezii specimens from different production regions are provided. A fragment of about 650 bp was amplified by PCR from each of the isolates analyzed. The analysis of the sequences demonstrated 100 % identity among the four specimens and 92–96.3 % identity with sequences of other species of Thecaphora. This is the first study on molecular sequences of this pathogen.  相似文献   

Biological control of botrytis bunch rot in organic wine grapes with the yeast antagonist Candida sake CPA‐1     

C. Calvo‐Garrido  P. A. G. Elmer  I. Viñas  J. Usall  E. Bartra  N. Teixidó 《Plant pathology》2013,62(3):510-519

The aim of this research was to confirm the efficacy of the yeast antagonist Candida sake CPA‐1 in suppressing botrytis bunch rot development, in an organic vineyard under Mediterranean conditions for two seasons, and compare its performance with that of two biologically based products currently registered for botrytis bunch rot control in New Zealand. In 2009, treatments applied were: commercial formulations of Ulocladium oudemansii (BOTRY‐Zen^®) and chitosan (ARMOUR‐Zen^®), C. sake CPA‐1 combined with the fatty acid‐based additive Fungicover^® and combinations of these products. All treatments were applied six times between early flowering and harvest and compared with an unsprayed control. In 2010, the treatments focused on C. sake and Fungicover and the number of applications was reduced from six to four. The population dynamics of U. oudemansii and C. sake were measured and wine quality tests were carried out in both seasons. Disease control achieved by C. sake treatments in 2009 were comparable to those achieved by BOTRY‐Zen and ARMOUR‐Zen. Applications of C. sake plus Fungicover between flowering and harvest significantly (P < 0·05) reduced botrytis bunch rot incidence and severity by 64% and 90%, respectively, compared with the untreated control in 2009, and by 67% and 89%, respectively, in 2010. Treatments did not adversely affect wine quality parameters after treated grapes were processed. Candida sake consistently provided effective control of botrytis bunch rot in grapes under different meteorological and disease pressure conditions, thereby improving its potential for future commercial applications.  相似文献   

The pyrethroid resistance status and mechanisms in Aedes aegypti from the Guerrero state,Mexico     

H. Angélica Aponte  R. Patricia Penilla  Felipe Dzul-Manzanilla  Azael Che-Mendoza  Alma D. López  Francisco Solis  Pablo Manrique-Saide  Hilary Ranson  Audrey Lenhart  Philip J. McCall  Américo D. Rodríguez 《Pesticide biochemistry and physiology》2013

Dengue is one of the most important vector-borne diseases worldwide and is a public health problem in Mexico. Most programs in dengue endemic countries rely on insecticides for Aedes control. In Mexico, pyrethroid insecticides (mainly permethrin and deltamethrin) have been extensively used over a decade as adulticides and represented a strong selection for insecticide resistance for dengue vectors in several parts of the country. We studied the type, frequency and distribution of insecticide resistance mechanisms in Aedes aegypti from six municipalities in the state of Guerrero selected on the basis of historically intense chemical control and a high risk for dengue transmission. Ae. aegypti eggs were collected from October 2009 to January 2010 using ovitraps. F₁ adults, emerged from these collections, were exposed to permethrin, deltamethrin and DDT in WHO diagnostic tests and showed high resistance levels to both pyrethroids and DDT. This was consistent with the presence of increased metabolic enzyme activities and target site insensitivity due to kdr mutations. Biochemical assays showed elevated esterase and glutathione S-transferase activities in the six municipalities. The V1016I kdr mutation on the IIS6 domain of the sodium channel gene was present in an overall frequency of 0.80. A second mutation, F1534C on the IIIS6 domain of the same gene was also detected, being the first report of this mutation in Guerrero. The multiple resistance mechanisms present in Ae. aegypti from Guerrero state represent a warning for the efficacy of the pyrethroid usage and consequently for the success of the dengue control program.  相似文献   

Report on the survey for Bursaphelenchus xylophilus and the occurrence of other Bursaphelenchus species in Hungarian coniferous forests     

Á. Tóth  M. Elekes 《EPPO Bulletin》2013,43(1):152-154

An ongoing official survey to detect the pine wood nematode Bursaphelenchus xylophilus, a quarantine pest, started in 2003 in coniferous forests in Hungary. Based on the results of the study from 2003–11, B. xylophilus has not yet been detected in Hungary. Two other Bursaphelenchus species (B. mucronatus and B. vallesianus) were identified in samples in 2009. Details of the survey and the measurements of B. mucronatus and B. vallesianus are provided.  相似文献   

Land-use and land-cover change processes in the Upper Uruguay Basin: linking environmental and socioeconomic variables   总被引：1，自引：0，他引：1  

de Freitas  Marcos Wellausen Dias  Santos  João Roberto dos  Alves  Diógenes Salas 《Landscape Ecology》2013,28(2):311-327

Landscape Ecology - Land-use and land-cover change affects both ecological and socioeconomic processes, motivating the integration of environmental and socioeconomic data to help understand this...  相似文献   

In vitro morphogenesis of juvenile Annona cherimola Mill,bud explants     

C. L. Encina  A. Barceló-Muñoz  A. Herrero-Castaño  F. Pliego-Alfaro 《The Journal of Horticultural Science and Biotechnology》2013,88(6):1053-1059

A micropropagation procedure for juvenile cherimoya (Annona clierimola Mill.) is described. Axillary shoot proliferation was obtained after culturing nodal sections with lateral buds in basal medium supplemented with either 0.66 μM BA or 1,36μM zeatin. For root induction shoots were pre-incubated for 7 d in basal medium supplemented with 1 gl^?1 activated charcoal, then cultured for 10 d (7 dark/3 light) on medium with 500 μM IBA, 58.4 mM sucrose, and 200 mg I“1 citric acid. Afterwards, shoots were transferred to the same medium without auxin and with the macroelements at half strength. Using this procedure, 95% of shoots rooted. The survival rate at the end of the acclimatization period was 70%.  相似文献   

The effect of ethephon on the growth and development of Liatris spicata     

S. BañóN  A. González  J. A. Fernández  J. A. Franco 《The Journal of Horticultural Science and Biotechnology》2013,88(6):851-855

Summary

The first experiment studied the effect of applying ethylene biosynthesis regulating compounds (ethephon 1000 ppm, silver thiosulphate 0.4 mM and cobalt chloride 5 mM) on the growth and development of Liatris spicata cv. Callilepis. The results pointed to the important growth retardant properties of ethephon and the increased number of flowering stems per corm which could be obtained by pre-planting application. A second experiment aimed to optimize ethephon treatment in Liatris by applying the growth retardant in different concentrations (100, 700, 1300, 1900 and 2500 ppm) at three stages of development: stage I (pre-planting), stage II (immediately after the formation of basal leaf rosette) and stage III (when the main shoot began its rapid longitudinal growth). Besides confirming the effects produced by ethephon in the first experiment, the results suggested that the application of high ethephon concentrations (1900 and 2500 ppm) during stage II of growth produced many miniature flowering stems coming from the principal or secondary shoot.  相似文献   

Nutrient uptake of pepino plants in soilless cultivation     

J. Fresquet  S. López-Galarza  San Bautista  C. Baixauli  J.M. Gisbert 《The Journal of Horticultural Science and Biotechnology》2013,88(3):338-343

Summary

This study addresses vegetative growth and the uptake of nutrients and their accumulation in the vegetative and reproductive tissues of pepino (Solanum muricatum Ait.). The study was conducted in soilless plastic house conditions, with a planting density equivalent to 25.000 plants ha^–1 during two seasons (1997–98 and 1998–99). The plants were sampled, fractionated into leaves, stems, fruits and roots, then dried and weighed and the macronutrient content was analysed every 30.d for 300.d after transplanting. The yield obtained was 2.kg of fruit per plant, which is 30% of the total dry weight. The relative growth rate (RGR) ranged from 0.0028 to 0.0451.d^–1. The highest N, K, Ca and Mg concentrations were recorded in the leaves, and the highest P concentration in the roots. The nutrient accumulation of this yield was 10.64, 1.12, 16.06, 10.0 and 1.58.g of N, P, K, Ca and Mg per plant respectively. The highest accumulation of all nutrients was found in the leaves. The nutrient with the highest specificuptake rate was K, which ranged from 0.0037 to 0.0838.g g^–1 d^–1.  相似文献