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131.
马尾松无性系种子园花期花量分析 总被引:9,自引:0,他引:9
调查了广西覃塘林场马尾松无性系种子园的花期和花量,着重探讨花期和花量对种子生产与子代群体遗传结构的影响。结果认为;无性系间和分株间的花期并不完全一致,但存在一程度的同步性。进入正常结实期的种子园存在“花粉相对不足”问题。少数几个无性系的雄球花或雌球花园内主占主导地位使种子园种子的遗传基础面临变窄的问题。 相似文献
132.
133.
中国水牛血红蛋白多态性及命名研究 总被引:2,自引:1,他引:2
采用醋酸纤维薄膜法首次系统分析了中国12个水牛地方类群1071头水牛的血红蛋白多态性。结果表明,中国水牛血红蛋白受Hb^A,Hb^B,Hb^C3个等位基因控制,其频率分别为0.0761,0.8852和0.0387,Hb^C基因系首次命名。聚类分析表明,中国水牛名地方类群间亲缘关系较近。 相似文献
134.
本文从众多方案中选出6个方案,作为邻水县1990、1995和2000年三个年段的系统模型决策方案,并对这些方案进行了多方面的分析,为系统规划设计和领导决策提供科学依据。 相似文献
135.
Zhou HF Xie C Jian R Kang J Li Y Zhuang CL Yang F Zhang LL Lai L Wu T Wu X 《Journal of agricultural and food chemistry》2011,59(7):3060-3065
Caper (Capparis spinosa L.) fruits have been widely used as food and folk medicine in the Mediterranean basin and in central and west Asia. In this study, two biflavonoids, isoginkgetin, and ginkgetin, together with three other flavonoids, were isolated from caper fruits. Their chemical structures were elucidated by spectroscopic analyses and comparison with literature. To our knowledge, isoginkgetin, ginkgetin and sakuranetin were identified in caper for the first time. Notably, it is also the first time that biflavonoids have ever been found in the Capparidaceae. Concentrations of the two biflavonoids were measured in caper fruits collected from four major growing areas in northwest China. The anti-inflammatory effects of the flavonoids from caper fruits were evaluated by secreted placental alkaline phosphatase (SEAP) reporter assay, which was designed to measure nuclear factor-kappa B (NF-κB) activation. Isoginkgetin and ginkgetin showed inhibitory effects in initial screen at 20 μM, while the effect of ginkgetin was much greater than that of isoginkgetin. In a dose-response experiment, the IC(50) value of ginkgetin was estimated at 7.5 μM, suggesting it could be a strong NF-κB inhibitor and worthy of study in vivo. 相似文献
136.
137.
巨噬细胞天然抗性蛋白1 (NRAMP1)可抑制结核分枝杆菌(Mycobacterium)和布氏杆菌(Brucella)等多种胞内寄生病原菌的感染,提高动物机体的抗病能力.本研究从秦川牛脾脏中克隆了NRA MP1基因的一种可变剪接体NRA MPI-ISO,序列分析表明,NRA MPI-ISO比NRAMP1多了第3个内含子,从而导致编码序列提前终止于第3个内含子,NRA MPI-ISO编码200个氨基酸.为了探索可变剪接体NRAMPI-ISO的表达情况,本研究分别构建了原核表达载体pET-41-NRA MPI-ISO和真核表达载体pEGFP-NRA MPI-ISO.原核表达载体pET-41 -NRA MPI-ISO可在不同浓度的IPTG诱导下在大肠杆菌BL21中高效表达.真核表达载体pEGFP-NRA MPI-ISO转染牛成纤维细胞后,EGFP-NRAMP 1-ISO融合蛋白分布在细胞核和细胞质中,而正常的NRAMP1蛋白只分布在细胞质的溶酶体膜周围.半定量RT-PCR检测表明,NRA MPI-ISO基因在心、脾脏、肺脏等组织有较高的表达,而在脑、胰、生殖嵴中的表达量相对较低.本研究为进一步研究NRA MPI-ISO基因生物学功能提供了重要信息. 相似文献
138.
The lethal and sublethal effects of chlorantraniliprole against Spodoptera exigua (Hübner) were evaluated under laboratory conditions by oral exposure of neonate larvae to the compound. The 72 h LC50 value of this insecticide to S. exigua was found to be 12.747 μg l−1. A progressive larval mortality of 24.32% for LC30 treatment and 42.61% for LC50 treatment was observed from 4th to 6th day after exposure, which resulted in the reduced pupation rates in exposure groups.
The sublethal effects of this chemical were indicated by prolongation of larval period, the increase of pupal weight and decrease
in hatch rate of egg. Chlorantraniliprole at LC30 and LC50 rate significantly delayed larval development; the developmental duration of surviving larvae was extended for 22.5 and 28.6%,
respectively, compared with that of control group. LC30 treatment increased the mean weight of pupa and induced to the production of heavier pupa (>150 mg). In LC50 treatment, heavier pupa also showed up but the mean weight of pupa was not influenced. The egg hatch rate in LC50 group was significant lower than that in control and LC30 groups. No significant differences in pupal duration, emergence rate, sex ratio, egg number per female, and longevity of
adults were observed among treatments. Chlorantraniliprole had exceptional activity against S. exigua according to concentration–response bioassay in laboratory, and the toxicities were primarily resulted from immediate lethality. 相似文献
139.
重组M蛋白间接ELISA检测猪繁殖与呼吸综合征病毒抗体 总被引:2,自引:0,他引:2
用纯化的猪繁殖与呼吸综合征病毒重组M蛋白作包被抗原,建立了检测猪繁殖与呼吸综合征病毒抗体的间接ELISA方法,并确立了ELISA最佳工作条件:抗原包被浓度为3.5μg/mL,37℃1h加4℃过夜,血清(1:40)和酶标SPA(1:80)分别在37℃温育1h,加底物溶液常温显色5min。经重复性试验、交叉试验、阻断试验等试验结果表明该方法重复性好、特异性强、敏感度高;与美国IDEXX公司试剂盒相比较,特异性和敏感性分别为96.3%和93.5%,无显著性差异。用建立的方法检测临床血清样品168份,总阳性率为39.9%。 相似文献
140.
Synthesis,fungicidal activity,and 3D-QSAR of pyridazinone-substituted 1,3,4-oxadiazoles and 1,3,4-thiadiazoles 总被引:18,自引:0,他引:18
A series of novel 5-[1-aryl-1,4-dihydro-6-methylpyridazin-4-one-3-yl] -2-arylamino-1,3,4-oxadiazoles, fungicidally active, were synthesized based on bioisosterism and tested in vivo against wheat leaf rust, Puccinia recondita. These compounds were shown to be fungicidally active, and their activity was influenced by the nature of the substituents. By using the three-dimensional quantitative structure-activity relationships (3D-QSAR) method of comparative molecular field analysis (CoMFA), we have studied the structure and activity relationship of the compounds containing both pyridazinone-substituted 1,3,4-thiadiazoles and pyridazinone-substituted 1,3,4-oxadiazoles. The 3D-QSAR modes gave good correlation between the variations on percent inhibition and the steric-electrostatic properties. The results are consistent with a common mode of action for the pyridazinone-substituted 1,3,4-thiadiazoles and the pyridazinone-substituted 1,3,4-oxadiazoles, which further confirms that the 1,3,4-oxadiazole ring is a bioisosteric analogue of the 1,3,4-thiadiazole ring. These offer important structural insights into designing highly active compounds prior to their synthesis. 相似文献