Management and Assessment of Ensiled Forages and High-Moisture Grain     

William J. Seglar  Randy D. Shaver 《Veterinary Clinics of North America: Food Animal Practice》2014

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Paleontology. Variation and early evolution     

Hunt G 《Science (New York, N.Y.)》2007,317(5837):459-460

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Genomics. Beyond nature and nurture     

Robinson GE 《Science (New York, N.Y.)》2004,304(5669):397-399

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谈农业转基因生物产品发展及其安全管理     

王立忠  胡雪飞 《辽宁农业职业技术学院学报》2002,4(3):34-35

着重介绍农业转基因生物产品的发展及其安全管理知识,使人们更多地了解这方面的内容,为作好农业转基因生物产品的安全管理工作奠定基础,使农业转基因生物产品更好地为人类服务.  相似文献   

Being Human: Ethics, Environment, and Our Place in the World. By Anna L. Peterson     

Gene Wunderlich 《Agriculture and Human Values》2003,20(3):323-325

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Estimating the number of true null hypotheses from a histogram of p values     

Dan Nettleton  J. T. Gene Hwang  Rico A. Caldo  Roger P. Wise 《Journal of Agricultural, Biological & Environmental Statistics》2006,11(3):337-356

In an earlier article, an intuitively appealing method for estimating the number of true null hypotheses in a multiple test situation was proposed. That article presented an iterative algorithm that relies on a histogram of observed p values to obtain the estimator. We characterize the limit of that iterative algorithm and show that the estimator can be computed directly without iteration. We compare the performance of the histogram-based estimator with other procedures for estimating the number of true null hypotheses from a collection of observed p values and find that the histogram-based estimator performs well in settings similar to those encountered in microarray data analysis. We demonstrate the approach using p values from a large microarray experiment aimed at uncovering molecular mechanisms of barley resistance to a fungal pathogen.  相似文献   

利用改进的复合区间作图法和F1代群体进行杉木的QTL作图   总被引：2，自引：1，他引：2  

施季森  童春发 《分子植物育种》2004,2(1):1-6

区间作图和复合区间作图最先是为近交群体而设计的QTL作图方法，现已得到了广泛的应用。本文将它们应用于林木的F1代群体的QTL作图，称为改进的区间作图和复合区间作图法。该方法考虑了林木F1代1：1分离位点的信息，不同于通常的“拟测交”作图法。采用该方法，本文利用两个杉木无性系句容0号杉和柔叶杉的AFLP分子标记遗传连锁图谱对杉木的13个数量性状进行了QTL定位。当似然比统计量的阀值取为13．82(对应于LOD为3．0)时，在两张杉木遗传连锁图谱上，共搜索到了25个QTL。在句容0号无性系遗传连锁图谱上，有5个QTL分布在4个连锁群上。在柔叶杉的遗传连锁图谱上，有20个QTL分布在3个连锁群上，其中第6连锁群上集中了高达13个QTL。这一新的QTL作图方法在作图精度上有了较大的提高。文中所有的计算都是使用Mathematica软件编程完成的。  相似文献   

A comparison of marker-assisted and phenotypic selection for high grain protein content in spring wheat   总被引：3，自引：0，他引：3  

John Davies  William A. Berzonsky  Gene D. Leach 《Euphytica》2006,152(1):117-134

Wheat grain protein content (GPC) is a primary end-use quality determinant for hard spring wheat (Triticum aestivum L.), and marker-assisted selection (MAS) could help plant breeders to develop high GPC cultivars. Two experiments were conducted using two populations developed by crossing low GPC cultivars (Ember) and (McVey) with (Glupro), which contains a high GPC QTL from Triticum dicoccoides (DIC). In one experiment, MAS and phenotypic selection (PS) were employed to select high GPC genotypes, and the selected genotypes were grown in six North Dakota (ND), USA environments. In a second experiment, molecular markers were used to select BC₂F₂ plants from each marker class for the DIC allele from each population. These plants were twice self-pollinated to produce BC₂F₄ plants, which were grown in single ND and Minnesota (MN) environments. Mean GPC was highest among lines using PS at two environments and not significantly different between MAS and PS in the other four environments. Lines presumably homozygous for DIC alleles had significantly higher GPC than their respective low GPC parents. The phenotypic GPC variation explained by the markers (r ²) was 30% at the ND and 15% at the MN environment. The use of PS was as effective as MAS in selecting for high GPC genotypes and more effective in some environments. This likely can be attributed to PS enabling selection for both the major QTL and other genes contributing to GPC. The use of molecular markers might be more advantageous for transferring the high GPC DIC QTL in a backcrossing program during parent development.  相似文献   

A comparison of genetic relationship measures in strawberry (Fragaria × ananassa Duch.) based on AFLPs, RAPDs, and pedigree data     

Chemda Degani  Lisa J. Rowland  James A. Saunders  Stan C. Hokanson  Elizabeth L. Ogden  Avi Golan-Goldhirsh  Gene J. Galletta 《Euphytica》2001,117(1):1-12

Nineteen of the major strawberry (Fragaria × ananassa Duch.) cultivars grown in the UnitedStates and Canada were examined for AFLP markerpolymorphisms. For the AFLP reactions, the EcoRI-ACC primer was used in combination with fourMseI primers (MseI-CAC, MseI-CAG,MseI-CAT, or MseI-CTT). Each set ofprimers produced 46–66 scorable fragments ranging insize between 50 and 500 bp. The polymorphic fragmentsproduced from each set of primers were more thansufficient to distinguish among all the cultivars,demonstrating the usefulness of AFLP markers forcultivar identification. Similarity coefficients werecalculated based on data from 228 AFLP markers anddata from 15 previously characterized RAPD markers. The RAPD markers had been specifically selected forfingerprinting purposes because they succesfullydistinguish 41 strawberry cultivars, including the 19cultivars analyzed in this study. Separatedendrograms were constructed based on analysis of theAFLP and RAPD marker data using a neighbor-joiningalgorithm. The dendrograms were compared and found tobe very different. Correlations between similaritycoefficients calculated from AFLP marker data,similarity coefficients calculated from RAPD markerdata, and coefficients of coancestry calculated frompedigree information were evaluated. Interestingly,a better correlation with the coefficients ofcoancestry was observed with the RAPD marker data thanwith the AFLP marker data.  相似文献   

Carotene and novel apocarotenoid concentrations in orange-fleshed Cucumis melo melons: determinations of β-carotene bioaccessibility and bioavailability     

Fleshman MK  Lester GE  Riedl KM  Kopec RE  Narayanasamy S  Curley RW  Schwartz SJ  Harrison EH 《Journal of agricultural and food chemistry》2011,59(9):4448-4454

Muskmelons, both cantaloupe (Cucumis melo Reticulatus Group) and orange-fleshed honeydew (C. melo Inodorus Group), a cross between orange-fleshed cantaloupe and green-fleshed honeydew, are excellent sources of β-carotene. Although β-carotene from melon is an important dietary antioxidant and precursor of vitamin A, its bioaccessibility/bioavailability is unknown. We compared β-carotene concentrations from previously frozen orange-fleshed honeydew and cantaloupe melons grown under the same glasshouse conditions, and from freshly harvested field-grown, orange-fleshed honeydew melon to determine β-carotene bioaccessibility/bioavailability, concentrations of novel β-apocarotenals, and chromoplast structure of orange-fleshed honeydew melon. β-Carotene and β-apocarotenal concentrations were determined by HPLC and/or HPLC-MS, β-carotene bioaccessibility/bioavailability was determined by in vitro digestion and Caco-2 cell uptake, and chromoplast structure was determined by electron microscopy. The average β-carotene concentrations (μg/g dry weight) for the orange-fleshed honeydew and cantaloupe were 242.8 and 176.3 respectively. The average dry weights per gram of wet weight of orange-fleshed honeydew and cantaloupe were 0.094 g and 0.071 g, respectively. The bioaccessibility of field-grown orange-fleshed honeydew melons was determined to be 3.2 ± 0.3%, bioavailability in Caco-2 cells was about 11%, and chromoplast structure from orange-fleshed honeydew melons was globular (as opposed to crystalline) in nature. We detected β-apo-8'-, β-apo-10', β-apo-12'-, and β-apo-14'-carotenals and β-apo-13-carotenone in orange-fleshed melons (at a level of 1-2% of total β-carotene). Orange-fleshed honeydew melon fruit had higher amounts of β-carotene than cantaloupe. The bioaccessibility/bioavailability of β-carotene from orange-fleshed melons was comparable to that from carrot (Daucus carota).  相似文献