Effects of additive iron on growth,tissue distribution,haematology and immunology of gilthead sea bream, <Emphasis Type="Italic">Sparus aurata</Emphasis>     

George Rigos  Alexandros Samartzis  Morgane Henry  Eleni Fountoulaki  Efthimia Cotou  John Sweetman  Simon Davies  Ioannis Nengas 《Aquaculture International》2010,18(6):1093-1104

The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet). Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200 ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300 ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point of view.  相似文献   

Structure—activity relationships of organotin biocides and Ceratocystis ulmi     

George Eng  Stephen P. Coddington  Laura L. Stockton  Alexander D. W. Acholonu 《Pest management science》1989,26(2):117-121

The effect of organotin compounds on the growth of Ceratocystis ulmi, the causative agent of Dutch elm disease, was studied in shake culture. Triphenyltin, (C₆H₅)₃SnX, tricyclohexyltin, (C₆H₁₁)₃SnX, and tributyltin, (C₄H₉)₃SnX, compounds were all effective in the inhibition of C. ulmi in vitro. The anionic group, X, did not play a major role in the inhibitory activities of these compounds, suggesting that the species involved in the inhibition is R₃Sn⁺ or the hydrated cation.  相似文献   

Mechanisms responsible for high levels of permethrin resistance in the house fly     

Jeffrey G. Scott  George P. Georghiou 《Pest management science》1986,17(3):195-206

The mechanisms responsible for > 6000-fold permethrin resistance in a pyrethroid-selected strain of house fly, Learn-PyR, were investigated. Through electrophysiological, in vitro metabolism, in vivo penetration and synergism studies it was demonstrated that the resistance mechanisms consisted of enhanced metabolic detoxification via the mixed-function oxidase (MFO) system, target-site insensitivity and decreased cuticular penetration. The major resistance mechanism was the MFO-mediated detoxification. The elevated MFO activity was correlated with higher levels of cytochrome P-450, cytochrome b₅ and NADPH-cytochrome c reductase activity. The kinetics of the latter showed similar K_m but greater V_max values in the Learn-PyR than in the susceptible strain, suggesting that the elevated activity was due to an altered amount, but not an altered form, of the enzyme. The Learn-PyR strain showed widely varying levels of resistance to the pyrethroids tested. Comparison of the pyrethroid structures with the resistance ratios revealed that resistance was highest in the presence of an unsubstituted phenoxybenzyl alcohol moiety. Substitution or certain modifications of the alcohol moiety reduced the level of resistance. Structure of the acid moiety or the presence or absence of an a-CN group did not affect the resistance level. These results are discussed with reference to the resistance mechanisms present.  相似文献   

Some fluorine-containing pheromone analogues     

Geoffrey G. Briggs  George R. Cayley  Glenn W. Dawson  David C. Griffiths  Ewen D. M. Macaulay  John A. Pickett  Mary M. Pile  Lester J. Wadhams  Christine M. Woodcock 《Pest management science》1986,17(4):441-448

Two analogues of the aphid alarm pheromone (E)-β-farnesene, a trifluorofarnesene and a difluoro-1-norfarnesene, were found to be highly active and were more readily detectable than the parent compound. For (Z)-hexadec-11-enal, a component of some lepidopteran sex attractant pheromones, replacement of the carbonyl oxygen with a difluoromethyl group to give a difluoroheptadecadiene resulted in loss of activity. A trifluoroacetoxyhexadecanolide was a more volatile analogue of the mosquito oviposition pheromone (?)-(5R,6S)-6-acetoxy-5-hexadecanolide and was highly active.  相似文献   

Production of ex vivo lipopolysaccharide-induced tumor necrosis factor-α, interleukin-1β, and interleukin-6 is suppressed by trans-resveratrol in a concentration-dependent manner          下载免费PDF全文

Jean-Francois Marier  Keguan Chen  Peter Prince  George Scott  Jerome R.E. del Castillo    Pascal Vachon 《Canadian journal of veterinary research》2005,69(2):151-154

Trans-resveratrol is a biologically active compound present in certain foods that has anti-inflammatory and anticancer properties. These beneficial effects are derived from both the immune system and cytokines. The purpose of this study was to determine the immunomodulatory effect of trans-resveratrol on the ex vivo production of inflammatory and anti-inflammatory cytokines stimulated by lipopolysaccharides (LPS). Trans-resveratrol (0, 0.01, 0.1, 1, and 10 microM) was added to blood samples from male Sprague-Dawley rats (n = 6) along with 100 U of LPS (Escherichia coli serotype, 055B5). The samples were then incubated for 4 h at 37 degrees C and centrifuged. Finally, concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6 in the plasma were analyzed using an enzyme-linked immunosorbent assay (ELISA). The production of inflammatory (TNF-alpha and IL-1beta) and anti-inflammatory (IL-6) cytokines was suppressed by trans-resveratrol in a concentration-dependent manner. These results support the hypothesis that the immunomodulatory effect of trans-resveratrol plays an important role in disease conditions that involve an overproduction of inflammatory cytokines.  相似文献   

Biological characteristics of maize dwarf mosaic potyvirus from Spain     

M. Angeles Achon  Marion Pinner  Vicente Medina  George P. Lomonossoff 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(7):697-705

Two isolates of maize dwarf mosaic virus (MDMV-Sp and MDMV-Spl) obtained from maize in the northeast of Spain were studied. Both isolates induced similar reactions on 6 sorghum cultivars, johnsongrass and oat (cv. Clintland), with the exception of MDMV-Sp which produced a different reaction on one sorghum cultivar. Thirty-three grass species were tested as possible hosts (16 previously untested) and 18 were found to be susceptible. Among those, eight were previously unidentified hosts for MDMV:Aegilops ovata, A. ventricosa, Avena barbata, Bromus alopecurus, B. diandrus, B. fasciculatus, Echinaria capitata andLolium rigidum. Both isolates were transmitted from maize to maize nonpersistently byRhopalosiphum maidis, R. padi, Schizaphis graminum andSitobium avenae. The virus was not seed-transmitted in Mo17 and B73 maize inbred lines. Pinwheels, scrolls and short curved laminated aggregates were observed in the cytoplasm of maize cells infected by MDMV-Sp or MDMV-Sp1. In addition, laminated aggregates were observed in cells infected by MDMV-Sp1.  相似文献   

Inheritance of fenvalerate and carbofuran resistance in colorado beetles—Leptinotarsa decemlineata (Say)—from North Carolina     

D. Craig Heim  George G. Kennedy  Fred L. Gould  John W. Van Duyn 《Pest management science》1992,34(4):303-311

Progeny from reciprocal F1 crosses and F1 backcrosses between fenvalerate-resistant and fenvalerate-susceptible Colorado beetles, Leptinotarsa decemlineata (Say), and between carbofuran-resistant and carbofuran-susceptible Colorado beetles were bioassayed to investigate the mode of inheritance of resistance to these chemicals. Bioassays of progeny from these crosses indicate that resistance to fenvalerate is inherited in a semi-recessive, sex-linked manner and carbofuran resistance is inherited in a partially dominant autosomal fashion. Log concentration/probit mortality lines and chi-square tests, however, indicate that multiple genes may be involved in resistance to both insecticides.  相似文献   

Insecticide resistance patterns among colorado potato beetle,leptinotarsa decemlineata (Say) (Coleoptera: chrysomelidae), populations in north carolina     

Ned M. French  D. Craig Heim  George G. Kennedy 《Pest management science》1992,36(2):95-100

A residual bioassay was used to determine insecticide susceptibility in populations of Colarado potato beetle, Leptinotarsa decemlineata (Say), in eastern North Carolina. Mean bioassay mortalities were subjected to correlation analysis to assess patterns of resistance among tested insecticides. Within insecticide classes, cross-resistance patterns were observed between carbaryl and carbofuran (carbamates), fenvalerate and permethrin (pyrethroids), and among azinphosmethyl, methamidophos, and phosmet (organophosphates). Significant associations between insecticides from different insecticide classes were concluded to be due to multiple resistance. Knowledge of multiple and cross-resistance patterns are discussed in relation to the bioassay as a tool for monitoring insecticide resistance.  相似文献   

The role of Eskimo hunters, veterinarians, and other biologists in improving the humane aspects of the subsistence harvest of bowhead whales     

O'Hara TM  Albert TF  Oen EO  Philo LM  George JC  Ingling AL 《Journal of the American Veterinary Medical Association》1999,214(8):1193-1198

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Absence of enhanced detoxication of permethrin in pyrethroid-resistant house flies     

Donald H. DeVries  George P. Georghiou 《Pesticide biochemistry and physiology》1981,15(3):242-252

The mechanisms of resistance to pyrethroids were studied in a permethrin-selected (147-R) strain of the house fly, Musca domestica L. Approximately 12-fold synergism was obtained with a mixture of (1R)-trans-permethrin:piperonyl butoxide (1:5) so that the resistance decreased from 97-fold to 22-fold. Tests with the esterase inhibitor S,S,S-tributyl phosphorotrithioate produced very little synergism in either the resistant (R) strain (1.6-fold) or the susceptible (S) strain (1.9-fold). An investigation of the microsomal components revealed that compared to the S strain, the R strain demonstrated twice as much cytochrome P-450 and cytochrome b₅ and double the rate of NADPH-cytochrome c reductase activity. In addition, the rate of p-nitroanisole O-demethylation was found to be six times greater in the R strain. An in vivo accumulation study showed that the R strain displayed a decreased rate of penetration of trans-[¹⁴C]permethrin. When treated at equitoxic doses the R strain was found to tolerate 50-fold more internal permethrin than the S strain. An in vitro metabolism study indicated that there was no difference between strains in the overall rate of metabolism of trans-[¹⁴C]permethrin. The evidence obtained supports the conclusion that several resistance factors are involved but that decreased sensitivity of the nervous system to the action of pyrethroids is the principal mechanism of resistance in the 147-R strain.  相似文献