无损检测桃子电特性的试验研究   总被引：16，自引：7，他引：16       下载免费PDF全文

胥芳  张立彬  周国君  贾灿纯  Luigi Ragni  Gennaro Vassalini 《农业工程学报》1997,13(1):202-205

利用智能ＬＣＲ测量仪和平板电极系统，用无损检测方法测定了桃子在储藏过程中电特性的变化。结果表明：在１２Ｈｚ～１００ｋＨｚ的频段内，桃子的最佳测试频段为１５ｋＨｚ以下，此时桃子随着储藏时间的增加，等效阻抗增大而相对介电常数和介质损耗因数减小；当桃子开始腐烂时，电特性数值出现了一个大的反复  相似文献   

p62/SQSTM1 expression in canine mammary tumours: Evolutionary notes     

Francesca Mariotti  Gian Enrico Magi  Alessandra Gavazza  Silvia Vincenzetti  Andrey Komissarov  Alex Shneider  Franco Maria Venanzi 《Veterinary and comparative oncology》2019,17(4):570-577

Recent studies highlighted the role of autophagy as a cardinal regulatory system for homeostasis and cancer‐related signalling pathways. In this context, the deregulated expression of p62 – Sequestosome1 (p62/SQSTM1) – a protein acting both as an autophagy receptor and signalling hub, has been associated with tumour development and chronic inflammation. Multiple clinical studies test drugs targeting autophagy, and even more research is on the way to clinical trials. However, no comparative investigations have been carried out to identify adequate preclinical models to assess p62‐based medicine. In veterinary oncology the role of p62 in cancer‐related pathways has been largely ignored. We compared p62 sequences in multiple organisms and found that canine p62 significantly diverges from the humans and from other animals sequences. Then, we chart by immunohistochemistry the expression levels of p62 in canine mammary tumours. A total of 66 tumours and 10 non‐neoplastic mammary samples were examined. The expression of p62 was higher in normal tissue and adenomas than carcinomas, with lowest levels of p62 protein detected in high grade carcinomas. In all cases examined the tumour stroma appeared to be p62‐negative. Taken together our results would suggest that in dogs the association between p62 expression and cancer cells overturns that reported in human breast carcinoma, where p62 accumulates in malignant cells as compared to normal epithelium. Thus, at least in canine mammary tumours, p62 should be not considered a tumour‐rejection antigen for an anti‐cancer immunotherapy.  相似文献   

Transnasal administration of a combination of dexmedetomidine,midazolam and butorphanol produces deep sedation in New Zealand White rabbits          下载免费PDF全文

Bruna Santangelo  Fabiana Micieli  Tiziana Mozzillo  Fabiana Reynaud  Francesco Marino  Luigi Auletta  Giancarlo Vesce 《Veterinary anaesthesia and analgesia》2016,43(2):209-214

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What is your diagnosis? Squash preparation from the lung of a hedgehog          下载免费PDF全文

Simone Manzocchi  Luigi Venco  Angela Di Cesare 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(4):715-716

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Interaction of food resources and landscape structure in determining the probability of patch use by carnivores in fragmented landscapes     

Alessio Mortelliti  Luigi Boitani 《Landscape Ecology》2008,23(3):285-298

Studies on the distribution of mammalian carnivores in fragmented landscapes have focused mainly on structural aspects such as patch and landscape features; similarly, habitat connectivity is usually associated with landscape structure. The influence of food resources on carnivore patch use and the important effect on habitat connectivity have been overlooked. The aim of this study is to evaluate the relative importance of food resources on patch use patterns and to test if food availability can overcome structural constraints on patch use. We carried out a patch-use survey of two carnivores: the beech marten (Martes foina) and the badger (Meles meles) in a sample of 39 woodland patches in a fragmented landscape in central Italy. We used the logistic model to investigate the relative effects on carnivore distribution of patch, patch neighbourhood and landscape scale variables as well as the relative abundance of food resources. Our results show how carnivore movements in fragmented landscapes are determined not only by patch/landscape structure but also by the relative abundance of food resources. The important take-home message of our research is that, within certain structural limits (e.g. within certain limits of patch isolation), by modifying the relative amount of resources and their distribution, it is possible to increase suitability in smaller/relatively isolated patches. Conversely, however, there are certain thresholds above which an increase in resources will not achieve high probability of presence. Our findings have important and generalizable consequences for highly fragmented landscapes in areas where it may not be possible to increase patch sizes and/or reduce isolation so, for instance, forest regimes that will increase resource availability could be implemented. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.  相似文献   

Differentiation and functional connection of vascular elements in compatible and incompatible pear/quince internode micrografts     

Espen L  Cocucci M  Sacchi GA 《Tree physiology》2005,25(11):1419-1425

Micrografts of internodes excised from in vitro grown pear plants (Pyrus communis L. cv. 'Bosc' (B) and cv. 'Butirra Hardy' (BH)) and quince (Cydonia oblonga Mill. East Malling clone C (EMC)), were cultured aseptically to test the effectiveness of their functional vascular reconnection in relation to incompatibility-compatibility relationships that these genotypes exhibit in the field. The incompatible heterograft (B/EMC) showed a marked delay in internode cohesion compared with the autografts (both B/B and BH/BH) and the compatible heterograft (BH/EMC). Even when fused, the translocation of [14C]-sorbitol from upper to lower internode was lower in B/EMC micrografts than in the other combinations. Epifluorescence studies performed with carboxyfluorescin, a specific phloem probe, indicated that the limited translocation was caused by a delay in the establishment of functional phloem continuity between the two internodes. In the B/EMC combination, new differentiated tracheary elements (TE) in the parenchyma tissue at the graft interface between the two internodes were not detected until 30 days after grafting, whereas in the BH/EMC heterograft and both autografts, new xylem connections appeared to cross the interface 20 days after grafting. Immunohistochemical detection (terminal nick-end labeling assay) of the number of cells undergoing nuclear DNA fragmentation at the graft interface confirmed that the limited and delayed TE differentiation in B/EMC heterografts was associated with a decrease in the activity of programmed cell death processes involved in the differentiation of TE.  相似文献   

Early detection and treatment of screw loosening in triple pelvic osteotomy     

Bogoni P  Rovesti GL 《Veterinary surgery : VS》2005,34(3):190-195

OBJECTIVE: To detect early screw loosening in triple pelvic osteotomy (TPO) and to evaluate the efficacy of retightening using fluoroscopic guidance and minimally invasive surgery to maintain acetabular alignment and achieve bone healing. STUDY DESIGN: Prospective clinical study. SAMPLE POPULATION: Sixteen dogs that had TPO. METHODS: Dogs (16) had TPO (21) by using pre-angled plates secured with 3.5 and 4.0 mm screws, with ischiatic or iliac cerclage, for osteosynthesis. In all but 1 TPO at least 1 screw was inserted into the sacral body (43 screws). The mean radiographic screw length inserted in the sacrum was 10.2 mm, and the mean percent sacral engagement was 22%. Dogs were examined clinically and radiographically immediately postoperatively, and at 10, 30, 60, and 90 days to evaluate screw position. Loose screws were retightened through stab incisions using fluoroscopy to locate the screw. RESULTS: TPO was performed without operative complications. At 10 days, 12 TPOs (57%; 11 dogs) had loose screws primarily located in the cranial aspect of the plate. This represented 20% (25) of the inserted screws. In 5 dogs, screw loosening resulted in medial rotation of the acetabular segment. All loose screws were retightened; 3 screws loosened again in 2 dogs and were detected at 60 days. However, the osteotomies healed with the planned acetabular rotation without further intervention. Screw tightening corrected the acetabular segment displacement. Screws correctly seated at 10 days did not subsequently loosen. Clinical and radiographic outcome was judged excellent in all dogs. CONCLUSION: Loose screws in TPO plates can be identified by 10 days postoperatively and retightened using fluoroscopic guidance to achieve acetabular realignment and healing without need for further surgery. CLINICAL RELEVANCE: Postoperative radiographic evaluation of screw position at 10 days after TPO is recommended to detect loose screws. Retightening loose screws should be considered as an alternative to TPO revision or confinement, especially in immature dogs.  相似文献   

Computed tomography-guided fine-needle aspiration and tissue-core biopsy of bone lesions in small animals     

Massimo Vignoli  DR. MED. VET.  SRV    Stefanie Ohlerth  DR. MED. VET.    Federica Rossi  DR. MED. VET.  SRV    Luigi Pozzi  PROF. DR. MED. VET.    Rossella Terragni  DR. MED. VET.    Daniele Corlazzoli  DR. MED. VET.    Barbara Kaser-Hotz  PROF. DR. MED. Vet 《Veterinary radiology & ultrasound》2004,45(2):125-130

In humans, free-hand computed tomography (CT)-guided biopsy is an accurate method to obtain a tissue sample. There are only a few reports of this technique in veterinary medicine. In the present study, 21 dogs and two cats underwent a free-hand CT-guided tissue-core biopsy (17 animals) or fine-needle aspiration (six animals) of a bone lesion. Two out of 17 tissue-core samples were also cultured. All 17 tissue-core biopsy samples were diagnostic (accuracy of 100%). Five out of six aspirates were diagnostic (accuracy of 83.3%). The overall accuracy was 95.7%. In one aspirate, cytologic quality was insufficient containing only blood. No major complications were encountered. Fourteen neoplastic, two infectious and six benign lesions were diagnosed. CT examination after intravenous contrast medium added useful information to avoid large vessels and to biopsy-viable tissue. Free-hand CT-guided tissue-core biopsy and aspiration appears to be a safe and very accurate procedure for use in the diagnosis of bone-associated diseases in small animals.  相似文献   

Are Circular External Fixators Weakened by the use of Hemispheric Washers?     

Marcellin-Little DJ  Roe SC  Rovesti GL  Bosio A  Ferretti A 《Veterinary surgery : VS》2002,31(4):367-374

OBJECTIVES: To compare the axial mechanical stability of 3 circular external fixators systems with and without hemispheric washers. STUDY DESIGN: Experimental study. METHODS: The axial stiffness and load necessary to produce 0.5 and 1 mm of displacement of 10 circular external fixator constructs from 3 manufacturers were tested on a materials testing machine. The constructs tested included the Small Bone fixator (SBF; Hofmann S.a.S., Monza, Italy), the IMEX ring fixator (IMEX Inc., Longview, TX), and the Multiplanar C-Fix (MCF; PanVet Distribuzione, Seriate, Italy). Five configurations were tested for each construct: (1) conventional nut fixation, (2) hemispheric washer fixation with connecting rods offset by 0, (3) 1, and (4) 2 holes, and (5) with a ring placed at maximum angulation. RESULTS: The loads resisted at 0.5 and 1 mm of displacement did not differ when frame configurations were compared (P =.25733 and.33769, respectively). The linear stiffness of the following configurations were decreasingly stiff: standard constructs, hemispheric washers with connecting rods perpendicular to rings, hemispheric washers with connecting rods offset by 1 hole, hemispheric washers with connecting rods offset by 2 holes, and ring offset in relation to bone model. The SBF constructs tested were 34% and 41% more rigid than the IMEX and MCF constructs tested despite the larger diameter of the connecting rods for the IMEX frames (6 mm) compared with the SBF frames (5 mm). The IMEX constructs tested were 6% more rigid than the MCF constructs tested. CONCLUSIONS: Adding hemispheric washers and angling connecting rods in relation to rings did not influence the loads resisted at 0.5 and 1 mm displacement but decreased construct stiffness. CLINICAL RELEVANCE: The use of hemispheric washers had minor effects on the biomechanical performance of fixator frames tested in this study when used to angle a ring in relation to connecting rods for circular external fixators.  相似文献   

Full season efficacy of moxidectin microsphere sustained release formulation for the prevention of heartworm (Dirofilaria immitis) infection in dogs   总被引：2，自引：0，他引：2  

Genchi C  Rossi L  Cardini G  Kramer LH  Venco L  Casiraghi M  Genchi M  Agostini A 《Veterinary parasitology》2002,110(1-2):85-91

The authors report the efficacy of an injectable, moxidectin sustained release (SR) formulation for the prevention of canine heartworm infection in endemic areas in northern and central Italy. Three field trials were carried out on a total of 324 dogs. Two hundred forty-three dogs were treated with moxidectin SR 6 months apart and 81 dogs (positive controls) with moxidectin tablets given monthly for 5 consecutive months during the risk season each year throughout the study. Results of testing for microfilariae and circulating adult female antigens were negative for all the experimentally treated dogs at the 6, 7, 11 and 19 months after the last injection. No adverse reactions to moxidectin SR were observed but a moderate pain at palpation and swelling (5-6 cm) at the injection site after the first treatment. In the study areas, prevalence of Dirofilaria immitis infection calculated by testing dogs which had no preventive treatment in the previous transmission season ranged from 33 to 63%. This study confirms the efficacy and safety of injectable, moxidectin SR formulation in the prevention of heartworm infection in dogs and demonstrates that the prophylactic efficacy lasts for the full season and strongly suggests that the product gives 1-year protection.  相似文献