全文获取类型
收费全文 | 10031篇 |
免费 | 625篇 |
国内免费 | 651篇 |
专业分类
林业 | 745篇 |
农学 | 718篇 |
基础科学 | 250篇 |
1298篇 | |
综合类 | 3088篇 |
农作物 | 720篇 |
水产渔业 | 626篇 |
畜牧兽医 | 2791篇 |
园艺 | 388篇 |
植物保护 | 683篇 |
出版年
2024年 | 30篇 |
2023年 | 92篇 |
2022年 | 204篇 |
2021年 | 247篇 |
2020年 | 241篇 |
2019年 | 224篇 |
2018年 | 210篇 |
2017年 | 323篇 |
2016年 | 263篇 |
2015年 | 295篇 |
2014年 | 361篇 |
2013年 | 482篇 |
2012年 | 590篇 |
2011年 | 626篇 |
2010年 | 555篇 |
2009年 | 497篇 |
2008年 | 609篇 |
2007年 | 559篇 |
2006年 | 472篇 |
2005年 | 432篇 |
2004年 | 341篇 |
2003年 | 474篇 |
2002年 | 688篇 |
2001年 | 666篇 |
2000年 | 418篇 |
1999年 | 228篇 |
1998年 | 45篇 |
1997年 | 61篇 |
1996年 | 38篇 |
1995年 | 50篇 |
1994年 | 48篇 |
1993年 | 39篇 |
1992年 | 49篇 |
1991年 | 41篇 |
1990年 | 58篇 |
1989年 | 52篇 |
1988年 | 37篇 |
1987年 | 41篇 |
1986年 | 30篇 |
1985年 | 36篇 |
1984年 | 51篇 |
1983年 | 27篇 |
1982年 | 26篇 |
1979年 | 29篇 |
1978年 | 28篇 |
1977年 | 27篇 |
1975年 | 25篇 |
1974年 | 37篇 |
1972年 | 30篇 |
1971年 | 37篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
71.
72.
旨在探究肌球蛋白结合蛋白C1(myosin binding protein C1,MyBPC1)对牛骨骼肌卫星细胞增殖与成肌分化的影响,为进一步研究MyBPC1在细胞分化和肌肉发育过程中的调控作用提供依据。本研究利用西门塔尔胎牛原代牛骨骼肌卫星细胞体外诱导成肌分化模型模拟牛骨骼肌的生长发育过程。采用qRT-PCR和Western blot检测MyBPC1的细胞时序表达谱。试验分为两组。在RNA水平每组4个重复,每个重复20 μL;在蛋白水平每组3个重复,每个重复15 μg。采用qRT-PCR和Western blot检测牛骨骼肌卫星细胞转染MyBPC1的过表达效果,并进一步检测细胞增殖期标志因子Pax7、Ki67以及细胞分化期标志因子MyHC、MyOG的表达变化情况,观察牛骨骼肌卫星细胞肌管形成状态。结果,MyBPC1在牛骨骼肌卫星细胞分化前后表达水平存在极显著差异,牛骨骼肌卫星细胞诱导分化后MyBPC1的mRNA和蛋白表达量均极显著高于增殖期(P<0.01)。过表达MyBPC1后,细胞分化形成的肌管数量明显多于对照组,增殖标志因子Pax7的mRNA水平和蛋白表达水平无显著差异,分化标志因子MyHC的mRNA水平和蛋白表达水平极显著高于对照组(P<0.01)。过表达MyBPC1可以促进牛骨骼肌卫星细胞体外成肌分化,为进一步开展MyBPC1对牛骨骼肌卫星细胞的调控机制奠定基础。 相似文献
73.
Thomas R. De Ridder Justine E. Campbell Cheryl Burke‐Schwarz David Clegg Emily L. Elliot Samuel Geller Wendy Kozak Stephen T. Pittenger Jennifer B. Pruitt Jocelyn Riehl Julie White Melissa L. Wiest Chad M. Johannes John Morton Pamela D. Jones Peter F. Schmidt Victoria Gordon Paul Reddell 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):415-429
74.
Pantu Kumar Roy Ahmad Yar Qamar Xun Fang Ghangyong Kim Seonggyu Bang Mahanama De Zoysa Sang Tae Shin Jongki Cho 《Reproduction in domestic animals》2021,56(2):342-350
Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus–oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 µg/ml) during in vitro maturation (IVM). Oocytes treated with 25 µg/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 µg/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress. 相似文献
75.
为研究日粮蛋白质水平对云南半细毛羊空怀母羊能量代谢的影响,试验选择25只云南半细毛羊空怀母羊,平均分为5个组,分别饲喂蛋白质水平为8.4%、10.0%、12.3%、13.6%、15.9%的日粮,用全收粪法进行消化代谢试验,试验期共19 d,预试期14 d,正试期5 d。结果表明:(1)日粮蛋白质水平对总能摄入量无显著影响(P > 0.05)。(2)从组1到组5粪能逐渐下降,且组3、组4、组5较组1分别降低了10.5%、11.4%、13.7%(P < 0.05),组5较组2降低了9.5%(P < 0.05)。(3)从组1到组5尿能逐渐增加,除组2与组1、组3间差异不显著(P > 0.05)外,其余组间差异极显著(P < 0.01)。(4)组4和组5总能消化率较组1极显著提高13.2%和11.8%(P < 0.01),其余各组差异不显著(P > 0.05)。组4总能代谢率较组1极显著提高9.8%(P < 0.01),其余各组差异不显著(P > 0.05)。对于消化能代谢率,组1和组2差异不显著(P > 0.05),组3、组4和组5依次显著降低(P < 0.05)。(5)蛋白质采食量与总能消化率、消化能代谢率间的回归方程分别为:Y=0.917X+45.872(R2=0.853)|Y=-0.370X+87.735(R2=0.899)。综上,日粮蛋白质水平与粪能呈负相关,与尿能呈正相关。日粮蛋白质水平为13.6%时,总能消化率和总能代谢率均较8.4%蛋白质水平组极显著提高。总能消化率随蛋白质采食量的提高而线性升高,消化能代谢率则随蛋白质采食量的提高而线性降低。
[关键词] 蛋白质|云南半细毛羊|空怀期|能量代谢 相似文献
76.
种植耐盐性强的桑树品种是土壤盐渍化地区生态修复的技术途径之一.选择桂桑优12、桂桑优62、抗青283×抗青10、桂桑5号、桂桑6号、桑特优2号共6个桑树品种的实生苗供试,将供试桑苗种植于质量浓度分别为0 g/L(CK)、2 g/L、4 g/L、6 g/L的NaCl溶液中,在历时28 d的盐胁迫试验期,每隔7d测定和统计各处理组桑苗的枝高、叶片数量、最大叶长、最大叶宽、受害叶片数量和枯死植株数量等,初步评价供试桑品种实生苗对盐胁迫的耐受能力.结果表明:在不同浓度盐胁迫处理组桑苗生长均较好的桑树品种是桂桑优12和桑特优2号;桑苗受盐害指数和死亡率最低的桑树品种均是桂桑优12.试验结果提示,6个供试桑树品种中桂桑优12是耐盐性最强的品种. 相似文献
77.
M. Muoz‐Rojas A. Jordn L. M. Zavala D. De la Rosa S. K. Abd‐Elmabod M. Anaya‐Romero 《Land Degradation \u0026amp; Development》2015,26(2):168-179
During the last few decades, land use changes have largely affected the global warming process through emissions of CO2. However, C sequestration in terrestrial ecosystems could contribute to the decrease of atmospheric CO2 rates. Although Mediterranean areas show a high potential for C sequestration, only a few studies have been carried out in these systems. In this study, we propose a methodology to assess the impact of land use and land cover change dynamics on soil organic C stocks at different depths. Soil C sequestration rates are provided for different land cover changes and soil types in Andalusia (southern Spain). Our research is based on the analysis of detailed soil databases containing data from 1357 soil profiles, the Soil Map of Andalusia and the Land Use and Land Cover Map of Andalusia. Land use and land cover changes between 1956 and 2007 implied soil organic C losses in all soil groups, resulting in a total loss of 16·8 Tg (approximately 0·33 Tg y−1). Afforestation increased soil organic C mostly in the topsoil, and forest contributed to sequestration of 8·62 Mg ha−1 of soil organic C (25·4 per cent). Deforestation processes implied important C losses, particularly in Cambisols, Luvisols and Vertisols. The information generated in this study will be a useful basis for designing management strategies for stabilizing the increasing atmospheric CO2 concentrations by preservation of C stocks and C sequestration. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
78.
79.
Effects of dietary lysozyme levels on growth performance,intestinal morphology,non‐specific immunity and mRNA expression in weanling piglets
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jiatao Zhu Xiaoxue Pang Zhengfeng Fang Yan Lin Lianqiang Che Shengyu Xu Jian Li Yiming Huang Xiang Su De Wu 《Animal Science Journal》2016,87(3):411-418
The aim of the present study was to determine the effect of dietary lysozyme levels on growth performance, gut health and non‐specific immunity of weanling piglets. A total of 150 weanling piglets were allocated to six treatments. The piglets were fed the same basel diet supplemented with 0, 30, 60, 90 and 120 mg/kg lysozyme as well as antibiotics for 28 days. From day 14 to day 28 of dietary treatment, piglets fed 90 mg/kg lysozyme had greater average daily gain than piglets fed control diet. During the whole experimental period, piglets fed 120 mg/kg lysozyme tended to have greater average daily gain than piglets fed control diet. Compared with piglets fed control diet, piglets fed diets containing antibiotics and 90 mg/kg lysozyme had greater villus height to crypt depth ratio in duodenum and jejunum. Additionally, dietary supplementation of 60 and 90 mg/kg lysozyme as well as antibiotics enhanced the phagocytic activity of peritoneal macrophages in piglets. In conclusion, dietary lysozyme can accelerate the growth of weanling piglets by improving gut health and non‐specific immunity and supplementing 90 mg/kg lysozyme is as effective as antibiotics (20 mg/kg colistin sulphate + 50 mg/kg kitasamycin) in improving the growth performance of weanling piglets. 相似文献
80.
Rearing conditions affected responses of weaned pigs to organic acids showing a positive effect on digestibility,microflora and immunity
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yalin Zhang Yumo Song Xiaoling Zhang Yan Lin Lianqiang Che Shengyu Xu De. Wu Bai Xue Zhengfeng Fang 《Animal Science Journal》2016,87(10):1267-1280
Three experiments were conducted to assess the response of weaned pigs to organic acid SF3, which contains 34% calcium formate, 16% calcium lactate, 7% citric acid and 13% medium chain fatty acids. Dietary treatments had no effect on growth performance of piglets (21‐day weaning) fed the commercial prestart diet for 1 week before receiving the experimental diets supplemented with SF3 at 0, 3 or 5 g/kg diet (Exp. 1), whereas diarrhea frequency averaged across a week was decreased by SF3 supplementation (5 g/kg diet) in piglets fed the experimental diets immediately after weaning (Exp. 2). In Exp. 3, piglets (28‐day weaning) were fed the control (containing pure colistin sulfate and enramycin, respectively, at 20 mg/kg diet) for 1 week and then were fed the control or SF3‐supplemented (5 g/kg diet) diet for 2 weeks. The SF3‐fed piglets had greater apparent ileal digestibility of calcium and dry matter, while also demonstrating greater overall gross energy, up‐regulated jejunal expression of sodium‐glucose cotransporter‐1 and transforming growth factor‐β, down‐regulated jejunal expression of tumor necrosis factor (TNF)‐α, higher ileal Lactobacillus, with lower total bacteria content, lower plasma TNF‐α but higher IgG levels than the control‐fed piglets. Collectively, SF3 consumption improved diarrhea resistance of weaned pigs by improving nutrient digestibility, piglet immunity and intestinal bacteria profile. © 2016 Japanese Society of Animal Science 相似文献