Powdery mildew resistance gene (<Emphasis Type="Italic">Pm</Emphasis>-<Emphasis Type="Italic">AN</Emphasis>) located in a segregation distortion region of melon LGV     

Xianlei?Wang  Guan?LiEmail author  Xingwang?Gao  Liman?Xiong  Wenlin?Wang  Rui?Han 《Euphytica》2011,180(3):421-428

Powdery mildew is one of the most important melon pathogens all over the world. So far, many genes conferring resistance to powdery mildew of melon have been described, but few of these have been finely mapped or cloned. Two F₂ populations derived from Ano2 × Hami413 and Ano2 × Queen were used to map the powdery mildew resistance gene by methods of Bulked Segregation Analysis (BSA), comparative genomics and Resistance Gene Analogues (RGA) mapping. It was found that the resistance to powdery mildew in Ano2 was conferred by a dominant gene, and the gene was named Pm-AN. The genetic analysis revealed that Pm-AN located between two codominant markers RPW and MRGH63B in linkage groupV. The genetic distances between Pm-AN and these two markers were 1.4–1.8 and 1.6–2 cM. No recombination was found between Pm-AN and markers ME/E1, SRAP23. Pm-AN was located in a RGA-rich region and cosegregated with the RGA marker MRGH5 and the resistance gene Vat. Synteny analysis showed that markers in this region were collinear between melon and cucumber. Segregation distortion was found in this region using both Ano2 × Hami413 and Ano2 × Queen F₂ populations, and the distortion was more distinct in Ano2 × Hami413 F₂ population. The center of segregation distortion was located in the RGA rich region harboring Pm-AN.  相似文献   

超声辅助提取丝状真菌被孢霉中油脂的工艺研究   总被引：1，自引：0，他引：1  

韩玉玺  于长青 《农产品加工．学刊》2011,(1):51-53

通过超声波辅助提取丝状真菌被孢霉中油脂的研究试验,确定影响提取的4个主要因素(超声波功率、提取温度、超声时间、料液比),以及丝状真菌被孢霉中油脂的超声波辅助提取的最佳萃取工艺。结果表明,超声波功率为120W,提取温度为45℃,超声时间为40min,料液比为1∶9,油脂平均得率为43.67%。  相似文献   

Effect of Fabrication Technology on the Performance and Structure of Stearic Acid/Attapulgite Composite Phase Change Materials     

WANG Yi  ZHENG Han  XIA Tian dong  FENG Hui xia  QIU Jian hui 《保鲜与加工》2011,(6):145-150

Two kinds of stearic acid (SA)/ attapulgite (ATTP) composite phase change materials (PCMs) were prepared for the latent heat thermal energy storage (LHTES) in buildings or other area by the methods of fusion and immersion, using SA as PCMs and ATTP supplied by Linze of Gansu province as supporting material. The structure, thermal properties, thermal reliability and heat storage or release performance of the composite PCMs were determined by scanning electron microscope (SEM), fourier transformation infrared (FTIR), differential scanning calorimetry (DSC) and thermal cycling test analysis technique. The results indicate that the preparation method has no effect on the structure and morphologies of the composites. Two kinds of composites have same structure due to the SA adsorbed in the surface of ATTP by physical reaction. The results also show that two form-stable composites PCMs have the optimal effect for preventing SA leak from the composite emerges when the maximum load proportion of SA in the composites were 38.64wt% and 29.13wt%. The latent heats and phase change temperature were measured as 68.71 J/g and 57.3℃ for the composite which was prepared by fusion method and 51.79 J/g and 55.2℃ for the composite fabricated by immersion method, respectively. The results of DSC, FTIR and thermal cycling test are all showing that two kinds of composites have good heat storage property but the composite prepared by fusion method has a better thermal and chemical stability.  相似文献   

稻田高效间作模式生态经济效益研究   总被引：5，自引：0，他引：5  

王缨  夏昌锐 《中国农业科学》1995,28(1):61-68

 本文总结了1985-1991年稻田间作模式随机区组试验和生产对比试验的有关产量、经济效益和生态效应的一系列资料。结果表明，本研究提出的各间作模式在创立高效农业上具有明显的优势，可作为我国发展高效生态农业的参考。  相似文献   

氮肥不同施用量和施用方式对冬小麦的影响     

王文颇  韩金玲  周印富  李彦生 《勤云标准版测试》2005,(6)

以超优66冬小麦品种为材料,采用双因素设计,对不同施氮量和施氮方式下冬小麦农艺性状和籽粒蛋白质含量进行了分析比较。试验结果表明,施氮量240kg/hm2和底施40% 拔节40% 孕穗20%的施肥方式组合为高产组合;施氮量240kg/hm2和底施40% 拔节40% 灌浆20%的施肥方式组合为高蛋白产量组合。  相似文献   

53种植物提取物对褐飞虱的杀虫活性测定   总被引：4，自引：0，他引：4  

邓业成  徐汉虹 《西南大学学报(自然科学版)》2005,27(5):668-671

用点滴法测定了53种植物66种提取物对褐飞虱的触杀活性。48h校正死亡率大于80％的植物提取物有10种,校正死亡率为50％～80％的植物提取物有10种。进一步测定了有触杀活性的12种植物提取物对褐飞虱的触杀毒力,其中银杏（Ginkgo biloba）叶、厚果鸡血藤（Millettia pachycarpa）种子、广西地不容（Stephania kwangsiensis）块根、黄连（Coptisc胁船瑚括）根的触杀毒力高,LD50值分别为0．0129,0．2519,1．5794和2．0578μg/头。  相似文献   

畜禽粪便成分快速测定方法的比较研究   总被引：3，自引：2，他引：3  

韩鲁佳  胡峥峥  阎巧娟  刘依  刘颖芳 《中国农业大学学报》2002,7(4):104-112

介绍了粪污计、电导仪、氨电极、粪污氮含量计量仪、反射计、快速在线畜禽粪便肥料成分检测系统等6种畜禽粪便肥料成分快速测定装置的工作原理和使用方法，阐述了上述各种快速测定装置的精度及适用范围，就各种方法的优劣进行了分析比较。与传统实验室法相比，6种快速测定方法简便、快速，具有一定的实用和推广价值，其中粪污计和电导笔成本较低，可完成氮、磷、钾和铵态氮含量的测定；快速在线畜禽粪便肥料成分检测系统能够较准确地测定粪便中铵态氮和磷的含量，但检测钾的准确度不高，且仪器设备昂贵，对运行环境要求高，稳定性差，对于固体粪便的检测能力较差。  相似文献   

施肥对紫花苜蓿分枝期光合特性的影响     

卫新菊  贾志宽  韩清芳  董志新 《勤云标准版测试》2006,(12)

目的研究不同施肥处理对3年生紫花苜蓿分枝期净光合速率、蒸腾速率、气孔导度等植物生理因子的影响。方法以加拿大进口紫花苜蓿品种——阿尔岗金为材料,采用美国LI-COR公司生产的LI-6400便携式光合作用测定系统进行测定。结果施氮10kg/hm2光合速率极显著地高于不施氮处理,施氮比不施氮光合速率高出8.07μmolCO2/m·2s(24.05%);施氮10kg/hm2对水分利用率的提高具有明显的作用,氮磷配施效果更好,以P40N10配施效果最好,P20N10次之;施磷20kg/hm2叶片蒸腾速率、气孔导度和胞间CO2浓度都最高,分别达到了14.88mmolH2O/m2·s、1.01cm/s和300μmol/mol结论苜蓿施氮10kg/hm2有助于提高分枝期叶片光合速率、水分利用效率。在施氮10kg/hm2水平下,施磷20￣40kg/hm2范围内,随施磷量的增加水分利用率提高。施磷20kg/hm2对提高叶片蒸腾速率效果显著,并且能明显提高气孔导度和胞间CO2浓度。  相似文献   

酶联免疫吸附分析法测定黄瓜中的甲基对硫磷     

韩丽君  钱传范  李文明  江树人  王小如 《勤云标准版测试》2005,(6)

采用自制多克隆抗体,考察了不同离子强度和不同甲醇含量对竞争反应的影响,并对反应体系进行了优化,最终建立了黄瓜中甲基对硫磷的残留检测ELISA方法。实验表明,甲基对硫磷对抗体竞争性结合的I50为0.13mg/L,方法检出限I20为6.7μg/L。除倍硫磷与杀螟硫磷表现了一定的交叉反应外,其它结构相类似的有机磷农药均无交叉反应。黄瓜样品经简单前与处理后分别采用ELISA和GC测定,用ELISA方法测得黄瓜中甲基对硫磷的添加回收率为83.10%￣93.46%,C.V为6.61%￣10.36%,最低检出限为0.003mg/kg,与GC测定数据基本相符,符合农药残留分析的要求。  相似文献   

黑白花奶牛初乳中G6PDH GOT与乳腺分泌机能关系的研究     

张志群  韩正康 《南京农业大学学报》1988,(4)

本文研究黑白花奶牛初乳中葡萄糖-β-磷酸脱氢酶(G6PDH),谷草转氨酶(GOT)、三氮醋酸沉淀蛋白(TCA-P)的动态变化,以及G6PDH,GOT与乳腺分泌机能的关系。结果表明:G6PDH,GOT,TCA-P在初乳7天的分泌模式为y=Ax~(-b)。初乳中每次挤奶乳样的G6PDH总量的7天平均值与日产乳量(3个月平均)存在显著正相关(r=0.7163,n=14,P<0.01);G6PDH与TCA-P的相关系数为r=0.7183(n=35).GOT与TCA-P的相关系数为r=0.8642(n=35)。本研究认为,黑白花奶牛初乳中的G6PDH、GOT活性可反映乳腺在初乳阶段的分泌机能,并可作为预测乳产量的指标。  相似文献