SOS response induction by beta-lactams and bacterial defense against antibiotic lethality     

Miller C  Thomsen LE  Gaggero C  Mosseri R  Ingmer H  Cohen SN 《Science (New York, N.Y.)》2004,305(5690):1629-1631

The SOS response aids bacterial propagation by inhibiting cell division during repair of DNA damage. We report that inactivation of the ftsI gene product, penicillin binding protein 3, by either beta-lactam antibiotics or genetic mutation induces SOS in Escherichia coli through the DpiBA two-component signal transduction system. This event, which requires the SOS-promoting recA and lexA genes as well as dpiA, transiently halts bacterial cell division, enabling survival to otherwise lethal antibiotic exposure. Our findings reveal defective cell wall synthesis as an unexpected initiator of the bacterial SOS response, indicate that beta-lactam antibiotics are extracellular stimuli of this response, and demonstrate a novel mechanism for mitigation of antimicrobial lethality.  相似文献   

Post-mortem changes in porcine M. longissimus studied by solid-state 13C cross-polarization magic-angle spinning nuclear magnetic resonance spectroscopy     

Bertram HC  Jakobsen HJ  Andersen HJ  Karlsson AH  Engelsen SB 《Journal of agricultural and food chemistry》2003,51(7):2064-2069

Solid-state (13)C cross-polarization (CP) magic-angle spinning (MAS) nuclear magnetic resonance (NMR) experiments are carried out for the first time on rapidly frozen muscle biopsies taken in M. longissimus in vivo and at 1 min, 45 min, and 24 h post-mortem from three pigs. Two of the pigs were CO(2)-stunned (control animals), and one was pre-slaughter-stressed (treadmill exercise) followed by electrical stunning to induce difference in metabolism post-mortem. (13)C resonance signals from saturated and unsaturated carbons in fatty acids, carboxylic carbons, and carbons in lactate and glycogen are identified in the solid-state NMR spectra. The (13)C CP MAS spectra obtained for post-mortem samples of the stressed, electrically stunned pig differ significantly from the post-mortem control samples, as the intensity of a resonance line appearing at 30 ppm, assigned to carbons of the methylene chains, is reduced for the stressed pig. This spectral difference is probably due to changes in lipid mobility and indicates altered membrane properties in the muscle of the stressed/electrically stunned animal when compared with the control animals already 1 min post-mortem. In addition, the post-mortem period changes in glycogen carbons can be estimated from the (13)C CP MAS spectra, yielding a correlation of r = 0.74 to subsequent biochemical determination of the glycogen content.  相似文献   

Growth,carcass yield and meat quality attributes of Red Maasai sheep fed wheat straw-based diets   总被引：1，自引：1，他引：0  

John G. Safari  Daniel E. Mushi  Louis A. Mtenga  George C. Kifaro  Lars O. Eik 《Tropical animal health and production》2011,43(1):89-97

Thirty-two castrated Red Maasai sheep (12.7 kg initial body weight, aged 12–18 months), were used in an 84-day experiment to evaluate diets based on treated straw upon growth performance, carcass yield and meat quality. The animals were blocked by weight into four similar groups and randomly allotted into four dietary treatments, with eight individually fed animals per treatment. The dietary treatments were ad libitum untreated wheat straw (UTS), wheat straw treated with urea and lime (TS), straw and ad libitum hay (UTSH), and TS and ad libitum hay (TSH). In addition, each experimental animal received 220 g/day (on as fed basis) of a concentrate diet. Treatment of straw increased (P < 0.05) dry matter intake (42.3 vs. 33.7 g/kg W ⁷⁵/day), energy intake (4.6 vs. 3.7 MJ ME/d) and the average daily gain (40.7 vs. 23.1 g). Animals on TS produced heavier (P < 0.05) carcasses (6.6 vs. 5.4 kg) with superior conformation than animals on UTS. Percentage cooking loss was higher in carcasses from animals fed TS compared to those from other diets. Except M. longissimus dorsi and M. semitendinosus, tenderness of muscles was not affected by diet but ageing of meat improved (P < 0.001) tenderness. Overall, straw treatment increased carcass yields with limited effects on meat quality attributes.  相似文献   

Molecular water motions of skim milk powder solutions during acidification studied by 17O and 1H nuclear magnetic resonance and rheology     

Møller SM  Whittaker AK  Stokes JR  Gidley MJ  Andersen U  Bertram HC 《Journal of agricultural and food chemistry》2011,59(18):10097-10103

The molecular motion of water was studied in glucono-δ-lactone-acidified skim milk powder (SMP) solutions with various pH values and dry matter contents. NMR relaxometry measurements revealed that lowering the pH in SMP solutions affected 17O and 1H T2 relaxation rates almost identically. Consequently, the present study indicates that the proteins present in the samples do not affect the 1H relaxation behavior markedly, even at relatively high SMP concentrations (15-25%). Comparison of rheological measurements and NMR measurements suggested that the collapse of κ-casein during acidification could contribute to the initial decrease in 17O and 1H relaxation rate in the pH range between 6.6 and 5.5 for 15% SMP and in the pH range between 6.6 and 5.9 for 25% SMP. However, below pH 5.5 the viscosity and 17O and 1H NMR relaxation rates did not correlate, revealing that the aggregation of casein micelles, which increases viscosity below pH 5.5, does not involve major repartitioning of water.  相似文献   

Premature death, risk factors, and life patterns in dogs with epilepsy     

Berendt M  Gredal H  Ersbøll AK  Alving J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(4):754-759

BACKGROUND: Epilepsy in dogs is often difficult to medically control, resulting in premature death of dogs with epilepsy. However, the risks of premature death are not known. HYPOTHESIS: Dogs with epilepsy have an increased risk of premature death as compared to a general population of dogs. ANIMALS: Sixty-three dogs diagnosed with epilepsy between 1993 and 1996 were included in this study. METHODS: A prospective longitudinal study of the population was performed from the diagnosis of epilepsy until the time of euthanasia, death, or a maximum of 12 years to investigate mortality and risk factors. Information about sex, onset, type, frequency, and control of seizures, remission of epilepsy, death, cause of death, and owner's perspective was collected and analyzed. RESULTS: The median age at death of dogs was 7.0 years. The life span of dogs in which euthanasia or death was directly caused by their epileptic condition was significantly shorter as compared with epileptic dogs that were euthanized because of other causes (P = .001). The median number of years that a dog lived with epilepsy was 2.3 years. Females lived longer with epilepsy than males (P = .036). Seizure type (primary generalized versus focal seizures) was not significantly associated with survival time. The remission rate of epilepsy (spontaneous remission and remission with treatment) was 15%. CONCLUSION AND CLINICAL IMPORTANCE: The diagnosis of epilepsy implies an increased risk of premature death. The prognosis for dogs with epilepsy is dependent on a combination of veterinary expertise, therapeutic success, and the owner's motivation.  相似文献   

Endoscopic‐Assisted Resection of a Pedunculated Uterine Leiomyoma With Maximal Tissue Preservation in a Cow and a Mare          下载免费PDF全文

Wilfried Schneeweiss MVM  Lea Krump MVM  Lucy Metcalfe BVSc  Eoin Ryan MVB  Marijke Beltman DVM  PhD  Diplomate ECAR  Hanne Jahns MVM  PhD  Diplomate ECVP 《Veterinary surgery : VS》2015,44(2):200-205

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Effect of differential N and S competition in inter- and sole cropping of Brassica species and lettuce on glucosinolate concentration     

Stavridou E  Kristensen HL  Krumbein A  Schreiner M  Thorup-Kristensen K 《Journal of agricultural and food chemistry》2012,60(25):6268-6278

Field and greenhouse pot experiments were conducted to evaluate the potential to use intercropping as an alternative method to increase glucosinolates in Brassicas by manipulating nitrogen (N) and sulfur (S) balance by intercropping with lettuce (Lactuca sativa L. var. capitata). In both experiments, four combinations of N and S fertilization were used. In the field experiment no effect of intercropping on the total glucosinolate concentration was found as the growing lettuce was strongly inhibited by the presence of broccoli (Brassica oleracea L. var. italic). In contrast to this, in the pot experiment both total and individual glucosinolate concentrations in red leaf mustard (Brassica juncea L.) increased by intercropping. Fertilization treatments influenced glucosinolate concentrations in both experiments, and an interaction between N and S fertilization was noticed.  相似文献   

Grain yield increase in cereal variety mixtures: A meta-analysis of field trials   总被引：1，自引：0，他引：1  

Lars P. Kiær  Ib M. Skovgaard  Hanne Østergård 《Field Crops Research》2009

Plant ecology theory predicts that growing seed mixtures of varieties (variety mixtures) may increase grain yields compared to the average of component varieties in pure stands. Published results from field trials of cereal variety mixtures demonstrate, however, both positive and negative effects on grain yield. To investigate the prevalence and preconditions for positive mixing effects, reported grain yields of variety mixtures and pure variety stands were obtained from previously published variety trials, converted into relative mixing effects and combined using meta-analysis. Furthermore, available information on varieties, mixtures and growing conditions was used as independent variables in a series of meta-regressions. Twenty-six published studies, examining a total of 246 instances of variety mixtures of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.), were identified as meeting the criteria for inclusion in the meta-analysis; on the other hand, nearly 200 studies were discarded. The accepted studies reported results on both winter and spring types of each crop species. Relative mixing effects ranged from −30% to 100% with an overall meta-estimate of at least 2.7% (p < 0.001), reconfirming the potential of overall grain yield increase when growing varieties in mixtures. The mixing effect varied between crop types, with largest and significant effects for winter wheat and spring barley. The meta-regression demonstrated that mixing effect increased significantly with (1) diversity in reported grain yields, (2) diversity in disease resistance, and (3) diversity in weed suppressiveness, all among component varieties. Relative mixing effect was also found to increase significantly with the effective number of component varieties. The effects of the latter two differed significantly between crop types. All analyzed models had large unexplained variation between mixing effects, indicating that the variables retrievable from the published studies explained only a minority of the differences among mixtures and trials.  相似文献   

The minimal enzyme cocktail concept for biomass processing     

Anne S. Meyer  Lisa Rosgaard  Hanne R. Sørensen 《Journal of Cereal Science》2009

Efficient generation of a fermentable hydrolysate is a primary necessity in the utilization of fibrous plant biomass as a feedstock in bioethanol processes. Enzyme catalyzed hydrolysis of cellulose and heteroxylans in biomass feedstocks each require multiple enzyme activities to achieve degradation to fermentable monosaccharides. The minimal enzyme cocktail concept concerns identification of the minimal number, the minimal levels, and the optimal combination of the best performing key monoactive enzymatic activities to meet this requirement. Two major hypotheses lie behind this concept: 1. That the native multi-component profiles of crude “wild type” cellulolytic and/or xylan degrading enzyme preparations are not optimal for degradation of cellulose in pre-treated lignocellulosic biomass nor for degradation of heteroxylans in hemicellulose-rich product streams; 2. That it is possible to replace crude multienzyme preparations with designed combinations of the minimal number of required enzyme activities for biomass processing. This paper outlines the current strategies employed and the stage of development of minimal enzyme cocktails for enzymatic hydrolysis of cellulose and arabino-xylan in complex, genuine biomass substrates. The available data demonstrate the feasibility of the concept and illustrate the potential efficacy improvements obtainable by use of designed minimal enzyme cocktails for pre-treated lignocellulosic and hemicellulose-rich biomass substrates.  相似文献   

Detection of singlet oxygen in blood serum samples of clinically healthy lambs and lambs suffering from alveld disease     

Hanne Hjorth Tønnesen  Ivar Mysterud  Jan Karlsen  Olav M. Skulberg  Carl M. M. Laane  Trond Schumacher 《Veterinary research communications》2010,34(4):347-357

Alveld is a disease in lambs of domestic sheep (Ovis aries L.), characterized by a combination of photosensitivity and liver damage. Generation of singlet oxygen play a major role in phototoxicity reactions. The compound phylloerythrin (phytoporphyrin) is so far assumed to be the main photodynamic agent in hepatogenous photosensitivity diseases in sheep. Phylloerythrin is a potent photosensitizer and an efficient source of singlet oxygen. The compound accumulates in the peripheral circualtion upon liver damage. Liver dysfunction is also likely to cause an increase in the blood level of bilirubin. Formation of singlet oxygen by bilirubin is reported. In the present work the photosensitizing potential of serum has been measured and related to the bilirubin- and phylloerythrin levels in lambs suffering from alveld and in clinically healthy controls. The singlet oxygen level of the serum was taken as a measure of the photosensitizing potential. The observed singlet oxygen values in serum from alveld lambs were significantly higher than the corresponding values observed in clinically healthy control lambs. This indicates that the serum of the alveld lambs contains an elevated concentration of photosensitizer. The singlet oxygen level was not correlated to the concentration of bilirubin or phylloerythrin. The results indicate that the photosensitizing mechanism is quite complex and may involve other sensitizer(s) than phylloerythrin.  相似文献