Reducing error in feline platelet enumeration by addition of Iloprost to blood specimens: comparison to prostaglandin E1 and EDTA          下载免费PDF全文

Harold W. Tvedten  Kerstin Bäcklund  Inger E. Lilliehöök 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(2):179-187

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Comparison of a Schmidt and Haensch refractometer and an Atago PAL‐USG Cat refractometer for determination of urine specific gravity in dogs and cats     

Harold W. Tvedten  Aså Norén 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(1):63-66

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Variation in vegetative parameters related to the nitrogen economy of wild barley,Hordeum spontaneum,in Israel     

H. Corke  E. Nevo  D. Atsmon 《Euphytica》1988,39(3):227-232

Summary Hordeum spontaneum, the wild progenitor of cultivated barley, has previously been examined in various studies as a germplasm resource in breeding for grain protein content and related nutritional traits.The nitrogen content and dry weight of leaf and stem (stem plus sheath) at anthesis, and the final grain size and grain protein content were measured in 33 H. spontaneum and two H. vulgare genotypes. H. spontaneum was generally higher in nitrogen content of leaves and stems, but lower in dry weight at anthesis. Consistent with previous reports, the H. spontaneum genotypes were considerably higher in grain protein than the cultivars. There was wide variation between and within populations of H. spontaneum suggesting that for breeding purposes lines combining high vegetative nitrogen content, dry weight and grain protein content can be selected.Incumbent of the Seagram Chair for Plant Science  相似文献   

A biologically-consistent stand growth model for loblolly pine in the Piedmont physiographic region, USA   总被引：1，自引：0，他引：1  

Oscar García  Harold E. BurkhartRalph L. Amateis 《Forest Ecology and Management》2011,262(11):2035-2041

A theory-influenced dynamical stand growth model formulation described well the behavior of thinned and unthinned loblolly pine plantations. A simplification containing few free parameters performed as well as a fully parametrized version. It seems particularly well suited to situations where available data is scarce. The stand model can be interfaced to additional climate, nutrition and carbon cycling modules for studying the effects of a changing environment.  相似文献   

Purification and identification of linoleic acid hydroperoxides generated by soybean seed lipoxygenases 2 and 3     

Fukushige H  Wang C  Simpson TD  Gardner HW  Hildebrand DF 《Journal of agricultural and food chemistry》2005,53(14):5691-5694

It has been known that lipoxygenase (LOX) isozymes exhibit differences in product formation, but most product information to date is for LOX 1 among soybean (Glycine max) LOX isozymes. In this study, LOXs 2 and 3 were purified and used to generate hydroperoxide (HPOD) products in an in vitro system using linoleic acid as a substrate in the presence of either air or O2. The products were analyzed to determine their stereoisomeric characteristics. The control (no enzyme) showed only low levels of hydroperoxide production and no stereoisomeric specificity. LOX 2 shows high specificity in product formation, producing roughly 4 times more 13-HPOD than 9-HPOD, nearly all of which was 13-S(Z,E)-HPOD. LOX 3 produced more 9-HPOD than 13-HPOD at approximately a 2:1 ratio. No single stereoisomer was predominant among LOX 3 products. These results demonstrate that different isozymes of LOX have characteristic product profiles in in vitro reactions.  相似文献   

Supercritical carbon dioxide extraction of oil and squalene from amaranthus grain     

He HP  Corke H  Cai JG 《Journal of agricultural and food chemistry》2003,51(27):7921-7925

Supercritical carbon dioxide (SC CO(2)) was used for the extraction of oil and squalene from Amaranthus grain. Very small amounts of oil could be extracted by SC CO(2) from undisrupted grains, although SC CO(2) possesses higher diffusivity. Grinding increased the extraction rate and oil yield, and smaller particle size gave higher extraction rate. The oil yield and initial extraction rate increased linearly with the increasing SC CO(2) flow rate from 1 to 2 L/min. Increasing the flow rate of SC CO(2) above 2 L/min resulted in only a slight increase of oil yield and extraction rate. In the pressure range of 150-250 bar, extraction decreased with increasing temperature at a constant pressure, whereas at a pressure of 300 bar, the extraction yield increased with increasing temperature. Possible reasons for this are discussed. Effects of temperature and pressure on squalene yield were different from those on oil yield. A good oil yield (4.77 g of oil/100 g of grain) was obtained at 40 degrees C and 250 bar. The highest squalene yield (0.31 g of squalene/100 g of grain) and concentration (15.3% in extract) were obtained at 50 degrees C and 200 bar, although the oil yield under this condition was low (2.07 g of oil/100 g of grain). The moisture content within 0-10% had little influence on yields of oil and squalene at 40 degrees C and 250 bar. Finally, the oil yield and the squalene concentration in the extracts by SC CO(2) were compared to those by solvent extraction.  相似文献   

Oil and squalene in amaranthus grain and leaf     

He HP  Corke H 《Journal of agricultural and food chemistry》2003,51(27):7913-7920

Amaranthus grain of 104 genotypes from 30 species was investigated for oil and squalene contents and fatty acid profiles. The overall average oil content in Amaranthus grain was 5.0%, ranging from 1.9 to 8.7%. Squalene concentration in extracted oils ranged from trace to 7.3%, with an average concentration of 4.2%. The average contents of three major fatty acids in Amaranthus grain were 22.2, 29.1, and 44.6% for palmitic, oleic, and linoleic, respectively. The average fat content in dried mature leaves of 45 Amaranthus genotypes was 1.63%, ranging from 1.08 to 2.18%. The squalene concentration in leaf lipid extracts averaged 0.26%, ranging from trace to 0.77%, which is much lower than that from seeds. The major fatty acids of leaf extracts were linolenic, linoleic, and palmitic. Linolenic ranged from 56.5 to 62.0% of total fatty acids; linoleic, from 15.5 to 24.7%; and palmitic acid, from 13.5 to 15.5%. As for the fatty acid compositions at different growth stages, fatty acid content in leaf lipid was lower in mature leaves than in young leaves. The saturated/unsaturated ratio decreased when the leaf grew to maturity. Principal component analysis (PCA) was carried out on compositional characteristics of grain. The first two components accounted for 70% of the total variance (38.3 and 21.7%, respectively). There was a positive correlation between oil content and squalene yield, and a negative correlations were found between linoleic and either of the other two major fatty acids, palmitic and oleic. The taxonomic relationship among the species was also elucidated by PCA.  相似文献   

Using Conferences and Workshops for Technology Training     

Reetz  Harold F. 《Precision Agriculture》2002,3(4):319-325

Conference and workshop formats provide excellent opportunities in continuing education for farmers and their advisers to stay abreast of new technology and practices for maintaining high-yield, high-profit management systems. Where possible hands-on opportunities should be included and proceedings published to help support the learning process. Communication and cooperation between industry and universities have been enhanced through these meetings. Since adoption of new technologies is often a team effort involving the farmers, input suppliers and other stakeholders, training programs should be designed to foster cooperation among them. The complexity of some of the new technologies makes the hands-on workshop and demonstration formats especially valuable.  相似文献   

Seeding and propagation of untransformed mouse mammary cells in the lung     

Podsypanina K  Du YC  Jechlinger M  Beverly LJ  Hambardzumyan D  Varmus H 《Science (New York, N.Y.)》2008,321(5897):1841-1844

The acquisition of metastatic ability by tumor cells is considered a late event in the evolution of malignant tumors. We report that untransformed mouse mammary cells that have been engineered to express the inducible oncogenic transgenes MYC and Kras(D12), or polyoma middle T, and introduced into the systemic circulation of a mouse can bypass transformation at the primary site and develop into metastatic pulmonary lesions upon immediate or delayed oncogene induction. Therefore, previously untransformed mammary cells may establish residence in the lung once they have entered the bloodstream and may assume malignant growth upon oncogene activation. Mammary cells lacking oncogenic transgenes displayed a similar capacity for long-term residence in the lungs but did not form ectopic tumors.  相似文献   

The mass disruption of Oort cloud comets     

Levison HF  Morbidelli A  Dones L  Jedicke R  Wiegert PA  Bottke WF 《Science (New York, N.Y.)》2002,296(5576):2212-2215

We have calculated the number of dormant, nearly isotropic Oort cloud comets in the solar system by (i) combining orbital distribution models with statistical models of dormant comet discoveries by well-defined surveys and (ii) comparing the model results to observations of a population of dormant comets. Dynamical models that assume that comets are not destroyed predict that we should have discovered approximately 100 times more dormant nearly isotropic comets than are actually seen. Thus, as comets evolve inward from the Oort cloud, the majority of them must physically disrupt.  相似文献