Identification of factor XI deficiency in Holstein cattle in Turkey     

Hasan Meydan  Mehmet A Yildiz  Fulya ?zdil  Yasemin Gedik  Ceyhan ?zbeyaz 《Acta veterinaria Scandinavica》2009,51(1):5

Background

Factor XI (FXI) is a plasma protein that participates in the formation of blood clots. Factor XI deficiency is autosomal recessive hereditary disorder that may be associated with excess bleeding in Holstein cattle.

Methods

In this study, 225 Holstein cows reared in Turkey were screened in order to identify FXI genotypes. DNA extractions were obtained from the fresh blood of the cows. Amplicons of FXI exon 12 were obtained by Polymerase Chain Reaction (PCR), and analyzed by 2% agarose gel electrophoresis stained with ethidium bromide. Additionally, all cows were confirmed by DNA sequencing to determine whether or not there was a mutant allele.

Results

Carriers of the FXI deficiency have two DNA fragments of 320 bp and 244 bp in size. The results of our study demonstrated that only four out of the 225 Holstein cows tested in Turkey carried the FXI deficiency. The frequency of the mutant FXI allele and the prevalence of heterozygous cows were found as 0.9% and 1.8%, respectively.

Conclusion

The DNA-based test determines all genotypes, regardless of phenotype or FXI activity. The mutation responsible for the FXI deficiency had not been detected in Holstein cattle in Turkey before prior to this study. The frequency of the mutant FXI allele needs to be confirmed by carrying out further analyses on cattle in Turkey and the selection programs should be developed to eliminate this genetic disorder.  相似文献   

Diprosopus, craniorachischisis, arthrogryposis, and other associated anomalies in a stillborn lamb     

Cihan Ka?ar  Kadir ?zcan  ?smet Tak??  Kutlay Gürbulak  Hasan ?zen  Musa Karaman 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):429-431

Congenital malformations with multiple anomalies have been described infrequently in the veterinary literature. A stillborn male crossbred lamb with diprosopus, craniorachischisis, and arthrogryposis was examined macroscopically and histopathologically in this study. The left head was smaller than the right head. Micrencephaly, agnathia, and a rudimentary tongue, which was adherent to the palate, were present in the left head. Micrencephaly, brachygnathia superior, and cleft palate were present in the right head. Cerebellar agenesis and spinal cord hypoplasia were observed. The cerebrums and the spinal cord were covered with a tapering membranous structure. Neural and dermal tissues were noted to intervene upon microscopic examination of this structure. Disorganization of neurons was observed in both cerebrums, though it was more severe in the left one. This case demonstrates many congenital defects occurring together in a lamb.  相似文献   

Immunohistochemical and qPCR determination of the expression and serum level of anti‐Müllerian hormone in pre‐pubertal,intact and ovarian remnant syndrome detected bitches     

Kubra Karakas Alkan  Ahmet Ceylan  Hasan Alkan  Dogukan Ozen  Alev Gurol Bayraktaroglu  Mustafa Kaymaz 《Reproduction in domestic animals》2019,54(7):979-986

The aim of this study was to determine the serum concentrations, ovarian presence and expression of anti‐Müllerian hormone (AMH) in pre‐pubertal, bitches with signs of ovarian remnant syndrome (ORS) and intact bitches. In addition, we aimed to verify the suitability of serum AMH concentrations for diagnostic purposes in sterilized bitches and/or in suspected cases of ORS in the field of veterinary medicine. For this purpose, 36 healthy female dogs divided into six groups: proestrus, oestrus, dioestrus, anoestrus, pre‐pubertal and ORS. Serum AMH concentrations were determined by electrochemiluminescence immunoassay, and ovarian presence and distribution of AMH was confirmed by immunohistochemical and qPCR techniques. According to the results of qPCR, while the expression values of AMH were at the highest concentrations in the proestrus and oestrus, there was a statistically significant decrease in these values at the later stages of the cycle (p < 0.05). According to hormone analysis, the serum AMH values of the ORS group had decreased significantly compared with the proestrus and oestrus (p < 0.05). Although serum AMH levels of ORS group were increased compared with anestrus and pre‐pubertal groups, this increase was statistically non‐significant (p > 0.05). Immunohistochemically, AMH expression was first observed in the granulosa cells of primordial follicles in folliculogenesis. Expression values were the highest in the proestrous and oestrus groups, but values from bitches in later stages of the cycle were statistically significant decrease in comparison with these groups (p < 0.05). As a result, AMH concentration and expression were found to be higher in proestrus and oestrus than in other periods (p < 0.05). In addition, the measurable level of AMH concentration in bitches with ORS is an indication that it can be used in the diagnosis of ORS.  相似文献   

Morphology and histology of the Anatolian bobcat (Lynx lynx) hard palate     

Hasan Hüseyin Ar&#x;  Sema Uslu 《Anatomia, histologia, embryologia》2019,48(4):290-295

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Determination of Pomological and Morphological Characteristics and Chemical Compositions of Local Apple Varieties Grown in Gumushane,Turkey     

Ozgun Kalkisim  Duygu Ozdes  Zuhal Okcu  Besim Karabulut  Hasan Basri Senturk 《Erwerbs-Obstbau》2016,58(1):41-48

The present study was conducted in order to determine the pomological and morphological characteristics and chemical compositions of some local apple varieties grown in Gumushane. As a result of field work in Gumushane province, sixteen different apples, which are Summer Apple, English apple, Green Belly, Black Belly and Yellow Belly Apples, Amasya Marble, Bey Apple, Chest Apple, Mahsusa, Arabian Girl, Willow Apple, Tav?anba??, Yellow H?d?r, Local Marble, Bride Apple, and Fatty Apple were collected in order to analyze. The average results of pomological and morphological properties and chemical compositions were obtained as following: fruit mass; 127.36 g, fruit width; 65.23 mm, fruit length; 57.30 mm, fruit stalk thickness; 2.17 mm, fruit stalk length; 17.63 mm, stalk pit depth; 12.75 mm, flower pit depth; 10.29 mm, fruit firmness; 13.79 kg/cm², seed house width; 15.89 mm, seed house length; 17.39 mm, number of seeds; 4.3, water soluble dry matter content; 14.53?%, ash content; 1.11?%, pH; 3.8, titratable acidity; 0.69 g/L, water content; 83.8?%, reducing sugar; 5.04 g/100 g, sucrose; 2.25 g/100 g, and the amount of total sugars; 7.29 g/100. The fruit peel color was determined by considering the light transmittance (L). The average L values were ranged between 112.06 and 66.32. On the other hand among the mineral elements, potassium and manganese amounts were determined as the highest and the lowest, respectively.  相似文献   

Two-year surveillance of tilapia lake virus (TiLV) reveals its wide circulation in tilapia farms and hatcheries from multiple districts of Bangladesh     

Partho Pratim Debnath  Jerome Delamare-Deboutteville  Mona Dverdal Jansen  Kornsunee Phiwsaiya  Afsana Dalia  Md. Abir Hasan  Saengchan Senapin  Chadag Vishnumurthy Mohan  Ha Thanh Dong  Channarong Rodkhum 《Journal of fish diseases》2020,43(11):1381-1389

Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV might be widespread, we executed a surveillance programme on tilapia grow-out farms and hatcheries from 10 districts of Bangladesh in 2017 and 2019. Among farms experiencing unusual mortality, eight out of 11 farms tested positive for TiLV in 2017, and two out of seven tested positive in 2019. Investigation of asymptomatic broodstock collected from 16 tilapia hatcheries revealed that six hatcheries tested positive for TiLV. Representative samples subjected to histopathology confirmed pathognomonic lesions of syncytial hepatitis. We recovered three complete genomes of TiLV from infected fish, one from 2017 and two from 2019. Phylogenetic analyses based on both the concatenated coding sequences of 10 segments and only segment 1 consistently revealed that Bangladeshi TiLV isolates formed a unique cluster within Thai clade, suggesting a close genetic relation. In summary, this study revealed the circulation of TiLV in 10 farms and six hatcheries located in eight districts of Bangladesh. We recommend continuing TiLV-targeted surveillance efforts to identify contaminated sources to minimize the countrywide spread and severity of TiLV infection.  相似文献   

Livelihood Improvements Through Agroforestry: Evidence from Northern Bangladesh     

Md. Abu Hanif  Ranjan Mitra Roy  Md. Shafiqul Bari  Polash Chandra Ray  Md. Shoaibur Rahman  Md. Faruq Hasan 《Small-Scale Forestry》2018,17(4):505-522

Agroforestry is one of the most sustainable land management systems practiced around the world due to the socioeconomic benefits that it brings to farmers. In Bangladesh, farmers practice agroforestry, applying indigenous knowledge. The present study was designed to identify the present status, management practices and its role in improving the livelihoods of farmers in northern Bangladesh. Data for the study were collected through quantitative and qualitative methods. A total of 29 tree and 38 agricultural crop species were planted by the102 farmers interviewed. Mangifera indica (relative prevalence 49%) is the most predominant species, followed by Eucalyptus camaldulensis (relative prevalence 35.4%). Farmers of northern Bangladesh plant trees in cropland for fruits (90%), fuel wood (87%) and timber production (79%). Fruit trees were planted with wider spacing while forest and fuel wood species were planted with narrower spacing. Farmer’s livelihoods improved enormously by practicing agroforestry as they have more access to food, fodder and fuel wood which is reflected by greater access to livelihood capitals (except social capital). However, farmers have experienced increased incidences of pests and diseases to the annual crops and trees. Agroforestry practices increases species diversity, ensure economic return and sustain farmer’s livelihoods.  相似文献   

Neuroprotective effects of dietary borax in the brain tissue of rainbow trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) exposed to copper-induced toxicity     

Gonca Alak  Arzu Ucar  Aslı Çilingir Yeltekin  Selim Çomaklı  Veysel Parlak  Ismail Hakkı Taş  Mustafa Özkaraca  Ahmet Topal  Esra Manavoğlu Kirman  İsmail Bolat  Muhammed Atamanalp  Hasan Türkez 《Fish physiology and biochemistry》2018,44(5):1409-1420

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A Modified Walkley-Black Method Based on Spectrophotometric Procedure     

Mohammad Bahadori  Hasan Tofighi 《Communications in Soil Science and Plant Analysis》2016,47(2):213-220

The Walkley-Black (WB) method, because of its simplicity and rapidity, has been widely used during the past 50 years for determination of soil organic carbon (SOC), but one of the main problems with WB method in operation is determination of titration end point by the observer. In the past several years some studies have been done to reduce the human role in determination of titration end point in WB method. In this study, in addition to the routine method of using an indicator for end point determination, a potentiometric and a spectrophotometric method was also employed for end point determination. The results indicated that these two methods are more accurate than the indicator method. Finally, a modified Walkley-Black (MWB) method employing a spectrophotometric procedure was proposed, which is more reproducible, easier in operation, and more economical.  相似文献   

MytiLec,a Mussel R-Type Lectin,Interacts with Surface Glycan Gb3 on Burkitt’s Lymphoma Cells to Trigger Apoptosis through Multiple Pathways     

Imtiaj Hasan  Shigeki Sugawara  Yuki Fujii  Yasuhiro Koide  Daiki Terada  Naoya Iimura  Toshiyuki Fujiwara  Keisuke G. Takahashi  Nobuhiko Kojima  Sultana Rajia  Sarkar M. A. Kawsar  Robert A. Kanaly  Hideho Uchiyama  Masahiro Hosono  Yukiko Ogawa  Hideaki Fujita  Jiharu Hamako  Taei Matsui  Yasuhiro Ozeki 《Marine drugs》2015,13(12):7377-7389

MytiLec; a novel lectin isolated from the Mediterranean mussel (Mytilus galloprovincialis); shows strong binding affinity to globotriose (Gb3: Galα1-4Galβ1-4Glc). MytiLec revealed β-trefoil folding as also found in the ricin B-subunit type (R-type) lectin family, although the amino acid sequences were quite different. Classification of R-type lectin family members therefore needs to be based on conformation as well as on primary structure. MytiLec specifically killed Burkitt''s lymphoma Ramos cells, which express Gb3. Fluorescein-labeling assay revealed that MytiLec was incorporated inside the cells. MytiLec treatment of Ramos cells resulted in activation of both classical MAPK/ extracellular signal-regulated kinase and extracellular signal-regulated kinase (MEK-ERK) and stress-activated (p38 kinase and JNK) Mitogen-activated protein kinases (MAPK) pathways. In the cells, MytiLec treatment triggered expression of tumor necrosis factor (TNF)-α (a ligand of death receptor-dependent apoptosis) and activation of mitochondria-controlling caspase-9 (initiator caspase) and caspase-3 (activator caspase). Experiments using the specific MEK inhibitor U0126 showed that MytiLec-induced phosphorylation of the MEK-ERK pathway up-regulated expression of the cyclin-dependent kinase inhibitor p21, leading to cell cycle arrest and TNF-α production. Activation of caspase-3 by MytiLec appeared to be regulated by multiple different pathways. Our findings, taken together, indicate that the novel R-type lectin MytiLec initiates programmed cell death of Burkitt’s lymphoma cells through multiple pathways (MAPK cascade, death receptor signaling; caspase activation) based on interaction of the lectin with Gb3-containing glycosphingolipid-enriched microdomains on the cell surface.  相似文献