Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis     

Takeshi Yokono  Yutaka Tamai  Tomonori Azuma  Yoh Sakuma  Kiyoshi Miura  Yasuo Kojima  Masahide Sunagawa  Masatake Ohmasa 《Journal of Wood Science》2000,46(6):480-484

The purpose of this research was to analyze the karyotype of the interspecific fusants of twoPleurotus species. Auxotrophic mutants derived from the cultivated strain ofP. ostreatus andP. cornucopiae were used. Protoplasts were fused electrically, and the fusants were selected under auxotrophic complementation. Esterase isozyme analysis showed that several fusants had isozyme bands originating from both parental strains, and others had unilateral isozyme bands. The fusant that had expressed isozyme bands of both parental strains showed chromosomal DNA bands of both of the parental strains in pulsedfield gel electrophoresis analysis. Despite the above results, the chromosomal composition of the fusants obtained by the pulsed-field gel electrophoresis did not exhibit all of the bands of both fusion parents.  相似文献   

Analysis of the electrophoretic karyotype ofFlammulina velutipes     

Doolyi Kim  Yutaka Tamai  Tomonori Azuma  Akira Harada  Akira Ando  Yoh Sakuma  Kiyoshi Miura 《Journal of Wood Science》2000,46(6):466-469

The karyotype ofFlammulina velutipes (Curt.: Fr.) Sing. was analyzed electrophoretically using contourclamped homogeneous electric field gel electrophoresis and hybridization with DNA probes. The chromosomal DNA from the monokaryon (Fv-4K) and the dikaryon (Fv-4) were resolved into six and eight bands, respectively. The sizes of the chromosomes ranged from 1.9 to 6.0 megabase (Mb) pairs. Each of the separated bands of chromosomal DNA was identified by use of five cloned probes. The number of these chromosomes was estimated to be 6 and 12, respectively; and the size of the entire genome was estimated to be about 20.1 and 38.6Mb, respectively. From a comparison of the hybridization patterns, the existence of allelic chromosomes of different sizes was deduced in the Fv-4 strain.  相似文献   

Unusual levels of phosphatidylcholine hydroperoxide in plasma, red blood cell, and livers of aromatic fish   总被引：1，自引：0，他引：1  

Janthira Kaewsrithong  Di-Fei Qiau  Toshiaki Ohshima  Hideki Ushio  Hideaki Yamanaka and  Chiaki Koizumi 《Fisheries Science》2000,66(4):768-775

  相似文献   

家蚕脂肪体合成蛋白质变化的研究   总被引：12，自引：6，他引：6  

徐豫松  徐俊良  川崎秀树 《蚕业科学》2000,26(4):239-243

应用双向电泳、同位素标记、放射自显影及计算机蛋白质电泳图谱分析技术研究了家蚕 5龄期和变态期脂肪体合成蛋白质的变化。结果表明 ,在 5龄中期脂肪体合成蛋白质的种类、分泌蛋白质的量、合成蛋白质的速度都达到最大值 ,合成脂肪体组织蛋白质在 5龄中期也快速增加 ,显示在 5龄中期脂肪体的蛋白质合成水平达到最大值 ,而熟蚕期后脂肪体合成蛋白质种类、分泌量及合成速度都下降 ,表明脂肪体蛋白质合成水平下降 ,在蛋白质合成水平上证明了家蚕脂肪体从幼虫期的合成、代谢等主要功能向变态期的贮藏功能转变。  相似文献   

Differential expression of myostatin and its receptor in the porcine anterior pituitary gland     

Yoshikazu TAKETA  Yasuhiro NAGAI  Hideki OGASAWARA  Shinichiro HAYASHI  Masato MIYAKE  Sachi TANAKA  Kouichi WATANABE  Shyuichi OHWADA  Hisashi ASO  Takahiro YAMAGUCHI 《Animal Science Journal》2008,79(3):382-390

Myostatin (MSTN), known as growth and differentiation factor 8 (GDF-8), is a member of the transforming growth factor β (TGF-β) superfamily that negatively regulates skeletal muscle mass. Myostatin binds with high affinity to the receptor serine threonine kinase activin receptor type IIB (ActRIIB). Activins that also belong to the TGF-β superfamily, stimulate follicle-stimulating hormone production in gonadotrophs and suppress growth hormone and adrenocorticotropic hormone production in somatotrophs and corticotrophs, respectively. The aim of the present paper was therefore to clarify the endocrine action of MSTN in adenohypophysis. The present study details the expression and cellular localization of MSTN and ActRIIB in porcine anterior pituitary gland. The mRNA of MSTN and ActRIIB was consistently expressed in RT-PCR. Immunohistochemistry of MSTN and specific hormones showed that MSTN localized in thyrotrophs and gonadotrophs, in which most of the MSTN immunoreactive cells were identified as thyrotrophs. The immunostaining of ActRIIB was restricted to corticotrophs. These results indicate that MSTN was mainly produced in thyrotrophs and its receptor, ActRIIB, was restrictively contained in corticotrophs. Interestingly, thyrotrophs immunoreactive for MSTN were frequently close to corticotrophs immunoreactive for ActRIIB. The present study suggests that MSTN from thyrotrophs may regulate corticotroph function as a paracrine mediator among the porcine anterior pituitary cells.  相似文献   

Genotypes and allele frequencies of buried SNPs in a bovine single‐nucleotide polymorphism array in Japanese Black cattle     

Shinji Sasaki  Eiji Muraki  Yoshinobu Inoue  Ryouhei Suezawa  Hideki Nikadori  Yuuichi Yoshida  Shouta Nariai  Ryoya Hideshima  Shiyunsuke Moriwaki  Ryotaro Nakashima  Katsuo Uchiyama  Kanako Yoshinari  Masayuki Takeda  Takatoshi Kojima 《Animal Science Journal》2019,90(12):1503-1509

Single nucleotide polymorphism (SNP) arrays are widely used for genetic and genomic analyses in cattle breeding; thus, data derived from SNP arrays have accumulated on a large scale nationwide. Commercial SNP arrays contain a considerable number of unassigned SNPs on the chromosome/position on the genome; these SNPs are excluded in subsequent analyses. Notably, the position‐unassigned SNPs, or “buried SNPs” include some of the markers associated with genetic disease. In this study, we identified the position of buried SNPs using the Basic Local Alignment Search Tool against the surrounding sequences and characterized the relationship between SNPs and genetic diseases in Online Mendelian Inheritance in Animals based on the genomic position. We determined the position of 285 buried SNPs on the genome and surveyed the genotype and allele frequencies of these SNPs in 5,955 individual Japanese Black cattle. Eleven SNPs associated with genetic disease, which contained five buried SNPs, were found in the population with the risk allele frequency ranging from 0.00008396 to 0.46. These results indicate that buried SNPs in the bovine SNP array can be utilized to identify associations with genetic disorders from large scale accumulated SNP genotype data in Japanese Black cattle.  相似文献   

Correction to: Dispersion and degradation of environmental DNA from caged fish in a marine environment     

Murakami  Hiroaki  Yoon  Seokjin  Kasai  Akihide  Minamoto  Toshifumi  Yamamoto  Satoshi  Sakata  Masayuki K.  Horiuchi  Tomoya  Sawada  Hideki  Kondoh  Michio  Yamashita  Yoh  Masuda  Reiji 《Fisheries Science》2019,85(6):1109-1109

Fisheries Science - In the original publication the text in right column of page 330, the sequences of primers were incorrectly published as.  相似文献   

Some factors affecting behaviour of boron in soil     

Togoro Hara  Makoto Tamai 《Soil Science and Plant Nutrition》2013,59(6):215-224

It has been reported by many workers that various soil properties influence the retention of boron added to soils, but there is little infomration on the relative importance of these properties to boron retention and there is something controversial in the published results regarding the effects of different soil properties such as organic matter content, soil reaction, available calcium content and texture on boron retention (15). The present study was undertaken to obtain more detailed informations on the relationships between boron adsorption and different properties of soils, and on comparative contribution of soil constituents such as organic matter, sesquioxides and inorganic colloids to boron adsorption of soils. In Japan, boron deficiency symptoms of crops often appear in the fields of volcanic ash soils, and many experiments on boron application have been conducted to amend the boron deficiencies of the soils. It is considered that volcanic ash soils may have special characteristics concerning boron retention in comparison with nonvolcanic ash soils. In the present study, therefore, some volcanic ash soils were also taken as samples in addition to non-volcanic ash soils to confirm their speciality to boron retention.  相似文献   

Allocation of 14C-carbon in two species of larch seedlings infected with ectomycorrhizal fungi     

Qu LY  Shinano T  Quoreshi AM  Tamai Y  Osaki M  Koike T 《Tree physiology》2004,24(12):1369-1376

The flow of labeled carbon in ectomycorrhizal and non-ectomycorrhizal seedlings of Japanese larch (Larix kaempferi Sarg.) and its F1 hybrid (Larix gmelinii Rupr. x L. kaempferi) was studied. Larch seedlings were grown in a greenhouse for 110 days with larch forest soil (FM) or Suillus grevillei (SM) inoculum, or in the absence of ectomycorrhizal fungi (NM). Shoots of colonized and NM seedlings were exposed to a pulse of 14CO2 for 1 h under natural light. Seedlings were harvested following 0, 6 and 24 h of exposure to 14CO2. At the final harvest, SM seedlings of Japanese larch and hybrid larch allocated 2.6 and 2.5% more 14C, respectively, to roots than NM seedlings. In contrast, FM seedlings of Japanese larch and hybrid larch allocated 6.5 and 18.0% more 14C, respectively, to the stem than NM seedlings. Of the total 14C detected in needle, stem and root fractions, FM and SM seedlings allocated a greater proportion than NM seedlings, perhaps because FM and SM seedlings had significantly (P < 0.05) higher photosynthetic rates than NM seedlings. As a result, FM and SM seedlings had greater dry masses than NM seedlings. Concentrations of nitrogen and phosphorus in FM and SM seedlings were significantly (P < 0.05) higher than in NM seedlings, as was stomatal conductance.  相似文献   

Photostable wood–inorganic composites prepared by the sol-gel process with UV absorbent     

Hisashi?MiyafujiEmail author  Hideki?Kokaji  Shiro?Saka 《Journal of Wood Science》2004,50(2):130-135

To enhance the photostability of wood against ultraviolet (UV) light, a UV absorbent, 2,2,4-trihydroxy-4-[2-hydroxy-3-(3-trimethoxysilylpropoxy)propoxy]benzophenone (BP), was applied to the reaction system for preparing SiO₂ wood–inorganic composites by sol–gel reaction. The BP–SiO₂ wood–inorganic composites obtained were examined for photostability by scanning electron microscope observations, color changes, and diffuse reflectance infrared fourier transform analyses. The results clearly indicated that compared with SiO₂ composites, BP–SiO₂ composites improved the photostability of wood. In addition, BP was stable against UV light without degradation so that the BP–SiO₂ composite should provide sustainable and high photostability of treated wood.Part of this report was presented at the 51st Annual Meeting of the Japan Wood Research Society at Tokyo, April, 2001  相似文献