A mesoscale iron enrichment in the western subarctic Pacific induces a large centric diatom bloom   总被引：2，自引：0，他引：2  

Tsuda A  Takeda S  Saito H  Nishioka J  Nojiri Y  Kudo I  Kiyosawa H  Shiomoto A  Imai K  Ono T  Shimamoto A  Tsumune D  Yoshimura T  Aono T  Hinuma A  Kinugasa M  Suzuki K  Sohrin Y  Noiri Y  Tani H  Deguchi Y  Tsurushima N  Ogawa H  Fukami K  Kuma K  Saino T 《Science (New York, N.Y.)》2003,300(5621):958-961

We have performed an in situ test of the iron limitation hypothesis in the subarctic North Pacific Ocean. A single enrichment of dissolved iron caused a large increase in phytoplankton standing stock and decreases in macronutrients and dissolved carbon dioxide. The dominant phytoplankton species shifted after the iron addition from pennate diatoms to a centric diatom, Chaetoceros debilis, that showed a very high growth rate, 2.6 doublings per day. We conclude that the bioavailability of iron regulates the magnitude of the phytoplankton biomass and the key phytoplankton species that determine the biogeochemical sensitivity to iron supply of high-nitrate, low-chlorophyll waters.  相似文献   

Characterization of neuron-like cells derived from canine bone marrow stromal cells     

Yasutaka Oda  Kenji Tani  Toshitaka Kanei  Tomoya Haraguchi  Kazuhito Itamoto  Hiroshi Nakazawa  Yasuho Taura 《Veterinary research communications》2013,37(2):133-138

Regenerative therapy using bone marrow stromal cells (BMSCs) has begun to be clinically applied in humans and dogs for neurological disorders such as spinal cord injury. Under appropriate conditions in vitro, BMSCs differentiate into neuronal cells, which may improve the effects of regenerative therapy. In this study, we evaluated canine neuron-like cells (NLCs) derived from BMSCs. We speculated on their suitability for neuro-transplantation from the point of view of their morphological features, long-term viability, abundant availability, and ability to be subcultured. Canine NLCs were differentiated as follows: third-passage BMSCs were maintained in pre-induction medium containing 2-mercaptoethanol and dimethylsulfoxide for 5 h, and then cells were transferred to neuronal induction medium containing fetal bovine serum, basic fibroblast growth factor, epidermal growth factor, dibutyryl cyclic AMP, and isobutylmethylxanthine for 7 or 14 days. Canine NLCs fulfilled the transplantation criteria and expressed markers of both immature neurons (nestin, 84.7 %) and mature neuronal cells (microtubule-associated protein-2, 95.7 %; βIII-tubulin protein, 12.9 %; glial fibrillary acidic protein, 9.2 %). These results suggest that canine BMSCs can be induced to differentiate into neuronal cells and may be suitable for neuro-transplantation. This study may provide information for improving cellular therapy for neurological diseases.  相似文献   

Estimation of evaporation from arid-irrigated region using MODIS satellite imagery     

Zhu Xue  Takeo Akae  Hidenori Morita 《Paddy and Water Environment》2013,11(1-4):185-192

For sustainable development of irrigated agriculture in arid regions, improvement of water use efficiency is essentially required to maintain current production levels and meet food and fiber for population growth in future. To achieve high water use efficiency, a key consideration is to reduce unnecessary soil water loss due to evaporation. In this article, regional daily evaporation over Hetao Irrigation District in a typical arid region during the irrigation period of 2009 was determined by a developed maximum surface temperature model combining Moderate Resolution Imaging Spectroradiometer (MODIS) satellite imagery. The results showed that maximum land surface temperature (LST) from MODIS satellite imagery was relatively higher in the western and middle parts than that of the eastern part of the district. At the same time, the mean minimum LST was shown somewhat higher in the eastern part. Mean daily evaporation was relatively higher in the eastern part, which showed water consuming is higher in the eastern part of the district. During the irrigation period of 2009, the total income water (irrigation water and rainfall) amount is 590.3 mm, and the outcome water (drainage discharge and evaporation) amount is 497.5 mm. The surplus of 92.8 mm in the irrigated season is considered to be consumed in winter season. Throughout the irrigated season, income and outcome almost equals each other. The daily evaporation distribution map could specify particular water consuming areas over the district where high daily evaporation may be occurred.  相似文献   

Nocturnal isoprene emission from mature trees and diurnal acceleration of isoprene oxidation rates near Quercus serrata Thunb. leaves     

Takafumi Miyama  Motonori Okumura  Yuji Kominami  Kennichi Yoshimura  Mioko Ataka  Akira Tani 《Journal of Forest Research》2013,18(1):4-12

Quercus serrata Thunb. ex Murray is a widespread deciduous oak in China, the Korean Peninsula, and Japan, and a strong isoprene emitter. Establishing accurate inventories of this species and estimating net carbon budgets, including biogenic volatile organic compounds (BVOC), necessitates detailed evaluation of BVOC emission and oxidation characteristics. Emissions of isoprene, the most abundant BVOC, presumably contribute to atmospheric chemistry through the formation of photochemical oxidants and secondary organic aerosols. We built an isoprene flux monitoring system to simultaneously reveal characteristics of the flux and fate of isoprene at multiple locations in Q. serrata forests. We used proton transfer reaction mass spectrometry (PTR-MS) and an automated closed chamber to measure isoprene emissions from soil and leaves in a warm-temperate Q. serrata forest. We used a relaxed eddy accumulation system with PTR-MS to simultaneously measure the canopy flux. In continuous foliage chamber measurements, we observed daily variations of isoprene emissions and continuous nocturnal emissions from leaves. Nocturnal emissions exceeded 25 % of total daily leaf emissions and were relatively high at sunset and low at sunrise. These results suggest that nocturnal emissions from mature trees may not be negligible. When leaf emissions were high in the daytime, the canopy isoprene flux tended to plateau at an upper limit. Observations of isoprene concentrations and gradients suggest that the plateau was caused by acceleration of isoprene oxidation, and sequential formation of secondary organic aerosols may occur near the leaf just after emission. Elucidation of these linkages may require continuous field measurements with a simultaneous multi-flux monitoring system.  相似文献   

Relationships between the addition rates of cellulase or glucose and silage fermentation at different temperatures     

Jian‐Guo ZHANG  Hidenori KAWAMOTO  Yi‐Min CAI 《Animal Science Journal》2010,81(3):325-330

The influence of the application rates of cellulase preparation and glucose on silage fermentation at different temperatures was studied with the straw of naked barley (Hordeum vulgare L. emand Lam) and guineagrass (Panicum maximum Jacq.). Addition rate of cellulase and glucose, temperature and their interaction had significant effects on pH value, lactic acid content, butyric acid content and propionic acid content of naked barley straw silage and significant effects on all the parameters of guineagrass silage (P < 0.01). Temperature and interaction had significant effect on acetic acid content (P < 0.05) and no significant effect on NH₃‐N content of naked barley straw silage (P > 0.05). Under all the temperatures, the pH values of barley straw and guineagrass silages were reduced by cellulase and glucose addition even at the lowest rate (P < 0.05), compared with their corresponding control. Lactic acid contents of silages were the highest within the same temperature and same additive when glucose and cellulase were added at the highest rates, whereas the effect of cellulase and glucose addition on butyric acid production varied with their application rates and silage storage temperature. The addition rate of restricting butyric acid fermentation was lower at 20°C than that at 30°C, and it was the lowest at 40°C where cellulase and glucose addition restricted butyric acid fermentation even at 0.1 g/kg and 10 g/kg, respectively, when compared to the control. While the addition rate was lower than the above level, cellulase and glucose addition also promoted butyric acid fermentation.  相似文献   

Seed formation in triploid loquat (Eriobotrya japonica) through cross-hybridization with pollen of diploid cultivars     

Shinji Kikuchi  Miwako Iwasuna  Aya Kobori  Yasunori Tsutaki  Akihiro Yoshida  Yuri Murota  Eisho Nishino  Hidenori Sassa  Takato Koba 《Breeding Science》2014,64(2):176-182

As the fruits of loquat (Eriobotrya japonica, 2n = 2x = 34) carry large seeds, the breeding of seedless loquat has long been a goal. The recent creation of triploid cultivars (2n = 3x = 51) and the application of gibberellins allow commercial production of seedless loquat, but the possibility of seed formation in triploid loquats has not been carefully investigated. Through crossing experiments and cytological observations of meiosis and pollen tube growth, we found that the triploid line 3N-N28 was essentially self-sterile, but developed seeds on pollination with pollen from diploid cultivars at rates of up to 5.5%. Almost half of the seedlings survived to 5 months, and carried diploid (2n = 34), tetraploid (2n = 68), or aneuploid chromosome numbers. Our results suggest that triploid loquat cultivars might retain the risk of seed formation. Protection from pollination by diploid cultivars or the development of new triploid cultivars will be necessary to ensure the production of seedless loquat fruits.  相似文献   

Effects of deep percolation on dissolved inorganic nitrogen exports from forested headwater catchments     

Kenta Iwasaki  Masanori Katsuyama  Makoto Tani 《Journal of Forest Research》2016,21(2):57-66

We examined the effects of deep percolation on dissolved inorganic nitrogen (DIN) exports from two adjacent weathered granite headwater catchments with different deep percolations of water in the Kiryu Experimental Watershed (KEW), Japan. The DIN output in streamflow was estimated from a regression equation between stream discharge and the DIN load, determined from both monthly sampling data and event-based sampling data. The range of deep percolation of DIN was estimated by multiplying observed DIN concentrations in bedrock groundwater by the deep percolation of water estimated from an annual water budget analysis. We found that the deep percolation of DIN corresponded to 34–76 % and ?18 to 8 % of the total DIN output in catchments where the deep percolation of water was 37–45 % and ?6 to 3 % of annual precipitation, respectively. This means that the deep percolation of DIN is not negligible when estimating the total output of DIN in the former catchment. Moreover, the fact that deep percolating water from upper catchments discharged into a lower catchment in KEW suggests that deep percolation of DIN affects downstream N budgets. Therefore, it is important to account for the deep percolation of DIN when evaluating N budgets in forested headwater catchments as well as downstream catchments.  相似文献   

Genomic variations among Bartonella henselae isolates derived from naturally infected cats     

Kabeya H  Maruyama S  Irei M  Takahashi R  Yamashita M  Mikami T 《Veterinary microbiology》2002,89(2-3):211-221

The purpose of this study was to understand the mechanisms of persistent infection with Bartonella henselae in cats. Blood samples were collected from three naturally infected cats for 24 months. These cats were confirmed to be persistently infected with B. henselae by serological and bacteriological examination. Relapsing bacteremia was found in all three cats with intervals of 3-19 months. Following the peaks of bacteremia, increases of specific antibody titer were observed in these cats. To examine the genetic differences among the isolates derived from the first and following bacteremia, the genome DNA patterns of the restriction enzyme fragment length polymorphism (RFLP) of the isolates were examined by pulsed field gel electrophoresis. The isolates derived from the first bacteremia showed an identical RFLP pattern in each of the three cats. The isolates derived from the following peaks, however, showed 1-3 of different RFLP patterns in these cats. Furthermore, the isolates showing different RFLP patterns from those of the first bacteremia were also detected at the following bacteremic peaks in all three cats examined. The 16S ribosomal RNA (rRNA) gene type of all isolates was found to be 16S rRNA type I. The emergence of genetically distinct organisms at various peaks of bacteremia may contribute to the establishment of persistent infection in the naturally infected cats.  相似文献   

Partial characterization of structure and function of a xylanase gene from the rumen hemicellulolytic bacterium Eubacterium ruminantium     

Hidenori TAGUCHI  Satoshi KOIKE  Yasuo KOBAYASHI  Isaac K. O. CANN  Shuichi KARITA 《Animal Science Journal》2004,75(4):325-332

A gene encoding for xylanase activity in the rumen hemicellulolytic bacterium Eubacterium ruminantium was cloned into pBR322 in Escherichia coli (E. coli ). The primary clone had a 5.7 kb insert produced by Eco RI partial digestion. Subcloning followed by sequencing allowed for the discovery that this enzyme has a glycosyl‐hydrolase family 10 catalytic domain with a family 9 carbohydrate binding module at C‐terminus and a region partially homologous to a family 22 carbohydrate binding module at N‐terminus. Cloned xylanase is specifically active against xylan and oligoxyloside to produce xylobiose and xylotriose, showing optimal pH and temperature at 7.0 and 50°C, respectively. Molecular size of the xylanase (91 kDa) was confirmed by zymogram analysis of the E. coli clone, which agreed with the predicted size from the DNA sequence. Functions of the two modules at C‐ and N‐termini were evaluated by using xylanase variants with and without the respective module and the C‐terminal module was found to be functional in binding to acid‐swollen cellulose and insoluble oat‐spelt xylan, whereas the N‐terminal module was inactive for binding them.  相似文献   

Survey of Cryptosporidium spp. and Giardia spp. infections in various animals at a zoo in Japan.     

M Matsubayashi  K Takami  Isao Kimata  Teruo Nakanishi  Hiroyuki Tani  Kazumi Sasai  Eiichiroh Baba 《Journal of zoo and wildlife medicine》2005,36(2):331-335

A total of 284 fecal samples of 89 species (43 mammalian species and 46 avian species) were examined for Cryptosporidium oocysts and Giardia cysts from 1999 to 2002. Each sample was collected at the zoo located at Osaka in Japan and examined by microscopy after performing the sucrose flotation method and by two immunofluorescent assay kits for detection of Cryptosporidium oocysts and Giardia cysts. Cryptosporidium spp. was found only in a raccoon dog (Nyctereutes procyonoides), and Giardia spp. was detected in a mandarin duck (Aix galericulata) and two ruddy shelducks (Tadorna ferruginea). In this study, the prevalences of these parasites were found to be low. However, these results suggested that the infected animals could serve as a source of contamination for surface water. This is the first report about the survey of Cryptosporidium spp. and Giardia spp. at a zoo in Japan.  相似文献