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81.
  1. The Pyrenean desman (Galemys pyrenaicus) is an endangered stream-dwelling insectivore endemic to the Iberian Peninsula and the north of the Pyrenees. It favours riffles over slow habitats such as runs or pools, yet it is still unclear whether habitat preference is based on prey availability or on other factors, such as mechanical constraints on its hunting habits.
  2. Desman diet and prey selection were analysed along the pristine Elama Stream and the slightly modified Leitzaran Stream in the Basque Country, Spain.
  3. In each stream the prey consumed were identified by metabarcoding 94 desman faeces, and prey availability characterized from 10 Surber samples taken in each habitat type (riffles, runs and pools). Invertebrates were sorted and identified, the biomass of each taxon was calculated, and their DNA was also extracted, amplified using PCR, and sequenced to build a reference database.
  4. Prey availability and diet varied between streams. Desmans positively selected shredders and invertebrates that live on the substrate, and selected against taxa that live in fine sediment, taxa with hard shells or those that are highly mobile. The diet of desmans was more selective in the Elama, the stream with better ecological status. Food availability did not differ among habitats, and therefore does not explain the preference of desmans for riffles; rather, mechanical constraints to deal with buoyancy and physical habitat heterogeneity were hypothesized to be the reason behind the habitat preference.
  5. The results suggest that promoting the formation of riffles in streams can improve the situation of some desman populations living in degraded habitats.
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83.
We describe the effect of a chemically characterized lipophilic extract obtained from Marthasterias glacialis L. against human breast cancer (MCF-7) and human neuroblastoma (SH-SY5Y) cell lines. Evaluation of DNA synthesis revealed that both cell lines were markedly affected in a concentration-dependent way, the SH-SY5Y cell line being more susceptible. Cell cycle arrest was observed, an effect induced by the sterol, ergosta-7,22-dien-3-ol, present in the extract. Morphological evaluation of treated cells showed the advent of lipid droplets and chromatin condensation compatible with apoptosis, which was confirmed by the evaluation of caspase-3 and -9 activities. Palmitic acid was the main compound responsible for this apoptotic effect by a ceramide-independent mechanism that involved endoplasmic reticulum (ER)-stress with upregulation of CCAAT/-enhancer-binding protein homologous protein (CHOP).  相似文献   
84.
There is an increasing body of evidence of the positive impact of several marine lipids on human health. These compounds, which include ω-3 polyunsaturated fatty acids, have been shown to improve blood lipid profiles and exert anti-inflammatory and cardioprotective effects. The high instability of these compounds to oxidative deterioration and their hydrophobicity have a drastic impact in their pharmacokinetics. Thus, the bioavailability of these compounds may be affected, resulting in their inability to reach the target sites at effective concentrations. In this regard; micro/nanoparticles can offer a wide range of solutions that can prevent the degradation of targeted molecules, increase their absorption, uptake and bioavailability. In this work we will present the options currently available concerning micro- and nanodelivery systems for marine lipids; with emphasis on micro/nanoparticles; such as micro/nanocapsules and emulsions. A wide range of bottom-up approaches using casein, chitosan, cyclodextrins, among others; will be discussed.  相似文献   
85.
Genetic Resources and Crop Evolution - We used genotyping-by-sequencing to investigate the evolutionary history of bere, the oldest barley variety still cultivated in Britain and possibly in all of...  相似文献   
86.
87.
The abundance of eastern white pine (Pinus strobus L.) has been significantly reduced across its distribution range over the past few centuries. The species’ regeneration dynamics is well documented in the centre of its range, but is poorly understood at the northern limit of continuous distribution. To address this knowledge gap, we quantified natural white pine regeneration in unmanaged mature stands, identified the most important variables influencing it, and evaluated the impact of damaging agents, namely white pine blister rust (Cronartium ribicola J.C. Fisch.), white pine weevil (Pissodes strobi Peck), and herbivory. We also quantified the influence of remnant stands and residual trees on the spatial distribution of regeneration in logged sites. The results reveal continuous but low recruitment in mature stands. The basal area of balsam fir (Abies balsamea (L.) Miller) had a strong negative effect on white pine regeneration. Regeneration was more abundant than expected on moister substrates, including moss, decaying wood and organic matter. White pine regeneration was noted in recently logged areas, where distance from remnant stands had a significant effect on the abundance of white pine regeneration. The northern limit of continuous distribution holds potential for white pine restoration, for example by preserving remnant white pine stands that can provide seed sources for natural regeneration in a shelterwood cut system, or in adjacent clearcut areas. This study illustrates that different management strategies should be used near northern range limits, where effects of site conditions and disturbance agents are different than in the center of a species’ range.  相似文献   
88.
A feeding experiment was conducted over 9 weeks with seven groups of 30 (fish per group) unpigmented gilthead seabream, Sparus aurata (L. 1875) (initial mean weight = 145.2 ± 12.3 g). Three experimental diets were prepared by adding to a basal diet free of carotenoid (final pigment content of around 40 mg per kg feed): (i) a biomass of the carotenogenic Chlorella vulgaris (Chlorophyta, Volvocales); (ii) a synthetic astaxanthin; and (iii) a mixture (1:1) of microalgal biomass and synthetic astaxanthin. At 3‐week intervals, five fish were sampled from each tank for total carotenoids analysis in skin and muscle. The carotenoid pigments (total amount = 0.4%) identified in the carotenogenic alga were lutein (0.3%), β‐carotene (1.2%), canthaxanthin (36.2%), astaxanthin, free and esterified forms (55.0%), and other pigments (7.3%). Carotenoid pigments were significantly deposited in the four skin zones studied during the feeding trial: the forefront between the eyes, the opercule, along the dorsal fin and in the abdominal area. In the muscle, regardless of the astaxanthin source, the amount of carotenoids measured was very low (less than 1 mg kg?1) and differences not significant. Moreover, no muscle pigmentation was evident, and there was no variation in the amount of carotenoid analysed in skin tissue, through the trial, for each treatment. It was concluded that supplementing the feed with C. vulgaris would be an acceptable practice in aquaculture to improve the market appeal of the gilthead seabream.  相似文献   
89.
The commercial furunculosis vaccine Aquavac Furovac 5 and an autogenous vaccine, based on the challenge strain, induced immune protection in turbot, Scophthalmus maximus (L.), as shown in challenge tests 120 days post-immunization by injection (relative percentage of survival, RPS = 72-99%). This protective effect lasted for at least 6 months post-immunization at appreciable levels (RPS = 50-52%). Neither the autogenous vaccine nor the commercial vaccine was able to induce significant levels of protection against Aeromonas salmonicida in turbot when administered by immersion. Antibody levels were high or moderate in fish vaccinated by injection with the different vaccines and very low in fish vaccinated by immersion. The field results show that delivering an oral boost after the primary vaccination by injection did not enhance protection of turbot against furunculosis and that water-based (autogenous vaccine) and oil adjuvanted (Alpha Ject 1200) vaccines administered by injection conferred similar levels of protection (RPS > 80%) in turbot.  相似文献   
90.
  1. Freshwater environments host roughly half of the world’s fish diversity, much of which is concentrated in large, tropical river systems such as the Amazon. Fishes are critical to ecosystem functioning in the Amazon River basin but face increasing human threats. The basic biology of these species, and particularly migratory behaviour, remains poorly studied, in part owing to the difficulty associated with conducting tagging studies in remote tropical regions.
  2. Otolith microchemistry can circumvent logistical issues and is an increasingly important tool for studying fish life histories. However, this approach is still new in the Amazon, and its potential and limitations to inform fish conservation strategies remain unclear.
  3. Here, otolith microchemistry studies in the Amazon are reviewed, highlighting current possibilities, and several key factors that limit its use as a conservation tool in the Amazon are discussed. These include the dearth of spatiotemporal elemental data, poor understanding of environment–fish–otolith pathways, and insufficient funding, facilities, and equipment.
  4. A research initiative is proposed to harness the potential of this technique to support conservation in the Amazon. Key aspects of the proposal include recommendations for internal and external funding, which are critical to acquiring and maintaining technical staff, cutting-edge equipment, and facilities, as well as fostering regular scientific meetings and working groups. Meetings can facilitate a systematic approach to investigating environment–otolith pathways, broadening the chemical baseline for most Amazonian tributaries, and exploring potential valuable elements.
  5. These outcomes are urgently needed to conserve biodiversity and ecosystem functioning in the Amazon, especially given threats such as widespread hydroelectric damming. The initiative proposed here could make otolith microchemistry an important, cost-effective tool to inform and foster conservation in the Amazon, and act as a template for other imperilled tropical river basins, such as the Mekong and the Congo.
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