Anthocyanins are water soluble pigments which have been proved to exhibit health benefits. Several studies have investigated their effects on several types of cancer, but little attention has been given to melanoma. The phytochemical content of nine different berry samples was assessed by liquid chromatography followed by electrospray ionization mass spectrometry (LC-ESI+-MS). Twenty-six anthocyanins were identified, after a previous C18 Sep-pak clean-up procedure. Chokeberry and red grape anthocyanins rich extracts (C-ARE and RG-ARE) were selected to be tested on normal and melanoma cell lines, due to their different chemical pattern. C-ARE composition consists of cyanidin aglycone glycosylated with different sugars; while RG-ARE contains glucosylated derivatives of five different aglycones. Both C-ARE and RG-ARE anthocyanins reduced proliferation, increased oxidative stress biomarkers and diminished mitochondrial membrane potential in melanoma cells, having no negative influence on normal cells. A synergistic response may be attributed to the five different aglycones present in RG-ARE, which proved to exert greater effects on melanoma cells than the mixture of cyanidin derivatives with different sugars (C-ARE). In conclusion, C-ARE and RG-ARE anthocyanins may inhibit melanoma cell proliferation and increase the level of oxidative stress, with opposite effect on normal cells. Therefore, anthocyanins might be recommended as active ingredients for cosmetic and nutraceutical industry.
In this study, the color and whiteness properties of fabrics knitted from ring, siro and compact core-spun yarns containing
metal wire were investigated. In general, an increase in the metal ratio of yarns causes a decrease in the whiteness and color
strengths of fabrics. In our study, spinning method had no statistically significant effect on either the Tw or K/S values of fabrics while the effect of spinning method on the whiteness index of fabrics was briefly observed. Fabrics knitted
from siro spun yarns showed higher whiteness properties than those of fabrics knitted from compact and ring spun yarns. This
might be explained by the superior covering effectiveness of the siro spinning method on metal wire. 相似文献
In order to determine the effects of autumn foliar application of boron (B) and/or urea on abortive flower ratio, yield, fruit weight, total soluble solid (TSS) and B and nitrogen (N) contents of reserves of non-irrigated apricot trees, field experiments were carried out between 2009 and 2012 in Malatya province of Turkey. The application of B and/or urea stimulated perfect flower development, B and N accumulation and resulted in significant yield increase. Boron, urea and B+urea applications increased fruit yield by 33.1%, 26.1% and 26.9%, decreased abortive flower ratio by 34.6%, 27.1% and 35.9% compared to the control, respectively. In addition, B and N contents of wood, bark and buds of apricot trees were significantly increased by B and/or urea treatments. It was also observed that B and N contents were the highest in bud compared to wood and bark in all treatments. The highest B (16.53 ppm) and N (1.56%) contents were determined to buds in B+urea treatment. The results of this study suggest that autumn foliar B and/or urea application have the potential to decrease abortive flower ratio and increase the yield and B and N contents of reserves of apricot trees under non-irrigated conditions. 相似文献
The Optium Xceed is a new hand-held meter for determining blood β-hydroxybutyrate (BHBA) and glucose in human medicine. The objective of this study was to compare BHBA and glucose results obtained using the hand-held meter with those results made with a laboratory method and to evaluate its usefulness as a cowside test in the diagnosis of subclinical ketosis (SCK) in dairy cows. Seventy-eight blood samples from clinically healthy Holstein cows between 5 and 60 days post-calving were analysed. BHBA and glucose values were significantly higher with the hand-held meter versus laboratory methods. Correlation coefficients (r) for BHBA and glucose with the Optium Xceed versus laboratory methods were 0.97 and 0.63, respectively. Based on Bland–Altman plot and Passing–Bablok regression, agreement between two methods was good for BHBA but the agreement for glucose was only fair. When SCK was defined as plasma BHBA levels 1200 μmol/L, the sensitivity and specificity of the hand-held meter ketone testing in determining SCK were 85% and 94%, respectively. Raising the threshold of the laboratory method to 1400 μmol/L, the sensitivity and specificity incremented to 0.90 and 0.98, respectively.In conclusion, the blood ketone-monitoring device can be used as a rapid and reliable diagnostic test to detect SCK under field conditions. 相似文献
The pine processionary moth Thaumetopoea wilkinsoni Tams is a serious pest on pine trees in southwestern Turkey. The efficacy of a naturally derived insecticide, spinosad, on fourth-fifth instar larvae of T. wilkinsoni was studied under laboratory conditions. The product exhibited strong larvicidal activity and at doses above 5 mg litre(-1) caused > 90% mortality in the fourth-fifth larval stages of the species after 72 h. At 72 h the LD50 and LD90 values were 3.26 and 5.69 mg litre(-1) respectively. The results showed that spinosad is highly effective on T. wilkinsoni larvae. 相似文献
The efficacy of administering doramectin after moxidectin treatment, which has previously proved only partially effective, was evaluated in five Angora rabbits naturally infested with Sarcoptes scabiei mange. Evaluations included physical examination for clinical signs of sarcoptic mange and collection of skin scrapings for determination of mites. The rabbits first received two subcutaneous injections, 10 days apart, of moxidectin 1% injectable solution at a dosage of 0.2 mg kg(-1) of bodyweight. Although moxidectin treatment resulted in clinical improvement within 10 days post initial injection, on days 10 and 35 post initial treatment live mites were present in skin scrapings. Administration of doramectin 1% injectable solution using the same route and dosage and at similar intervals to moxidectin led to complete disappearance of signs of scabies and parasitological cure in all rabbits. 相似文献
Six rabbits naturally infested with Psoroptes cuniculi were treated topically on the skin at the base of the neck with 0.5 mg kg(-1) of 0.5% pour-on eprinomectin for cattle, twice at 14-day intervals. Efficacy of the drug was based on the disappearance of clinical signs and the absence of live mites for a period of 6 weeks. Clinical improvement was seen within 3 days of the first application; however, complete recovery of clinical signs and elimination of mites in 5/6 rabbits did not occur until the end of the study. No adverse reactions attributable to eprinomectin treatment were observed during the observation period. Results of this trial indicated that eprinomectin was partially effective in the treatment of psoroptic mange in rabbits. 相似文献
From 2002 to 2006, plant growth promoting effects of Bacillus M3, Bacillus OSU-142 and Microbacterium FS01 were tested alone or in combination on apple (Malus domestica L.) cv. Granny Smith in terms of yield, growth and nutrient composition of leaves in the province of Malatya, Turkey. The presence of M3 and/or OSU-142 and/or FS01 combinations stimulated plant growth and resulted in significant yield increases in Granny Smith. Root inoculation of PGPR strains significantly increased cumulative yield (26.0–88.0%), fruit weight (13.9–25.5%), shoot length (16.4–29.6%) and shoot diameter (15.9–18.4%) compared with the control. In addition, all nutrient element contents (N, P, K, Mg, Ca, Fe, Mn and Zn) investigated in Granny Smith, except Mg was significantly affected by bacterial applications compared with the control. The highest N content (3.38%) was obtained from OSU-142 + FS01 application. Phosphorus contents of leaves of Granny Smith increased from 0.29% in the control to 0.42, 0.37, 0.36, 0.36 and 0.35% by M3, M3 + FS01, M3 + OSU-142, OSU-142 and M3 + OSU-142 + FS01 application, respectively. All bacterial applications significantly increased Ca content of leaves. The highest K, Fe, Mn, Cu and Zn content of leaves were obtained from OSU-142, M3, FS01, M3 and M3 + FS01 applications, respectively. The results of this study suggest that Bacillus M3 and/or OSU-142 and/or Microbacterium FS01 in combination have the potential to increase the yield, growth and nutrition of apple trees. 相似文献
Objective: To determine the effects of clodronate on vitamin D3‐induced hypercalcemia in dogs. Design: Prospective experimental study. Settings: University research laboratory. Animals: Fourteen healthy intact adult male and female mixed breed dogs. Interventions: Dogs received 7.5 mg of vitamin D3/kg of body weight once orally and were randomly assigned to 2 groups of 7 dogs each. Dogs in the saline control group were given intravenous infusions of 150 mL 0.9% NaCl solution 24 hours after vitamin D3 administration. Dogs in the clodronate group were given an infusion of 4 mg/kg of clodronate in 150 mL 0.9% NaCl solution 24 hours after vitamin D3 administration. Measurements and main results: Clinical signs of vitamin D3 toxicosis were evaluated 48 hours after ingestion of vitamin D3. Dogs that were given clodronate had significantly lower serum calcium (Ca), phosphorus (P), urea, and Ca × P values than dogs in the control group on days 4, 7, and 12 after administration. Additionally, alkaline phosphatase activity was significantly lower in the clodronate group compared with dogs in the control group on days 4 and 7. Conclusions: Parenteral administration of clodronate, a biphosphonate compound and osteoclastic activity inhibitor, may be a useful therapy when administered within the first 24 hours after ingestion of toxic doses of vitamin D3. 相似文献
This study aimed to determine the effect of flunixin meglumine treatment during and after the transfer of in vivo produced embryos to Angus (cows) and Holstein (cows and heifers) breeds of cattle on pregnancy rate. Holstein cows were used as donors in the study. A double dose of prostaglandin F2α was administered to the recipient animals for synchronization. Uterine flushing was performed in donors on day 7 after artificial insemination. A total of 295 transferable embryos were obtained. These embryos were transferred to Angus cows (n = 85), Holstein heifers (n = 80) and Holstein cows (n = 130). After the transfer, these animals were divided into three subgroups. The first subgroup (TI) was administered flunixin meglumine during embryo transfer, and the second subgroup (TII) was administered flunixin meglumine both during embryo transfer and on days 8 and 9 after the transfer. The third subgroup (TIII) was not administered anything and it was considered the control group. Pregnancy examination of the recipients was performed on days 30–35 after the transfer using real-time ultrasonography. The pregnancy rates after embryo transfer were found to be 43.52% in Angus cows, 42.5% in Holstein heifers, and 24.61% in Holstein cows (p < .05). When the animals were not classified according to breed, the pregnancy rates in subgroups TI, TII and TIII were found to be 29.29%, 45.10% and 29.79%, respectively (p < .05). In addition, the pregnancy rates were higher in TII and TIII subgroups of Angus cows and Holstein heifers compared to that of Holstein cows (p < .05). As a result, the pregnancy rates obtained after embryo transfer in Angus cows and Holstein heifers were found to be higher than that in Holstein cows. In addition, it was concluded that the administration of flunixin meglumine during and during/after embryo transfer has a positive effect on pregnancy rates in Angus cows and Holstein heifers. 相似文献
