Intracranial pressure monitoring in normal dogs using subdural and intraparenchymal miniature strain‐gauge transducers     

Beverly K. Sturges  Peter J. Dickinson  Linda D. Tripp  Irina Udaltsova  Richard A. LeCouteur 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):708-716

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Abortion in dairy cattle with advanced twin pregnancies: Incidence and timing     

Irina Garcia‐Ispierto  Fernando Lpez‐Gatius 《Reproduction in domestic animals》2019,54(Z4):50-53

This study examines incidences and timing of abortion in Holstein Friesian dairy cows with advanced twin pregnancies in north‐eastern Spain. The study population consisted of 1,194 twin pregnancies in two herds recorded from 2010 to 2015: 522 bilateral and 672 unilateral. The presence of live twins was determined by transrectal ultrasonography between 55 and 61 days post‐AI and pregnancy confirmed 60 days later. Abortion was recorded in 278 (23.3%) cows before Day 260 of pregnancy: 7 (1.3%) in bilateral and 271 (40.3%) in unilateral twin pregnancies. Using binary logistic regression procedures the following were excluded as risk factors for abortion: herd, year, heat stress (temperature‐humidity index values >72), lactation number and right versus left side for unilateral twins. Based on odds ratios, cows carrying unilateral twins were 49.9 (right unilateral twins) and 49.3 (left unilateral twins) times more likely to suffer abortion than cows with bilateral twins. The average time of abortion for unilateral twins was 173 ± 32 days and ranged from 135 to 249 days, 43% of abortions occurring between 135 and 154 days of gestation. The authors suggest prevention or reduction of unilateral twin pregnancies by follicular puncture and drainage of subordinate follicles at AI or manual rupture of the amniotic vesicle of one of the two twins at the time of pregnancy diagnosis.  相似文献   

Correlation of electrophoretic urine protein banding patterns with severity of renal damage in dogs with proteinuric chronic kidney disease          下载免费PDF全文

Jessica A. Hokamp  Sidney A. Leidy  Irina Gaynanova  Rachel E. Cianciolo  Mary B. Nabity 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(3):425-434

Background

Urine protein loss is common in dogs with chronic kidney disease (CKD). Currently available noninvasive means of evaluating CKD in dogs cannot accurately predict the severity of glomerular and tubulointerstitial damage. Electrophoretic analysis of urine proteins can indicate the compromised renal compartment (glomerular vs tubular), but extensive evaluation of protein banding pattern associations with histologic damage severity has not been performed in dogs.

Objectives

We aimed to evaluate electrophoretic banding patterns as indicators of the presence and severity of glomerular and tubulointerstitial damage in dogs with naturally occurring, predominantly proteinuric CKD.

Methods

We performed a retrospective study using urine and renal tissue from 207 dogs with CKD. Urine protein banding patterns were correlated with histologic severity of renal damage. Sensitivity and specificity of banding patterns for the detection of glomerular and tubulointerstitial damage were determined.

Results

Banding patterns were 97% sensitive and 100% specific for the detection of glomerular damage and 90% sensitive and 100% specific for the detection of tubulointerstitial damage. Correlations between composite banding patterns and the severity of renal damage were strong, while glomerular banding patterns correlated moderately with glomerular damage severity, and tubular gel scores correlated weakly to moderately with the severity of tubulointerstitial damage.

Conclusions and clinical importance

Urine protein banding patterns are useful for the detection of glomerular and tubulointerstitial damage in dogs with proteinuric CKD.  相似文献   

Photosynthetic Responses of Nitrate-Fed and Nitrogen-Fixing Soybeans to Progressive Water Stress     

Elisaveta Kirova  Nikolina Tzvetkova  Irina Vaseva  Georgi Ignatov 《Journal of plant nutrition》2013,36(3):445-458

The effect of nitrogen (N) source (nitrogen fixation or nitrate assimilation) and progressive water stress on pigment content, carbon assimilation and changes in the activity of certain photosynthetic (Rubisco and phosphoenol pyruvate carboxilase) and photorespiratory enzymes (glutamate synthetase and glycolate oxidase) during vegetative development of soybean plants was studied. Glycolate oxidase declined by 13% in nitrogen-fixing plants under water deficit, and increased in nitrate-fed ones. Nodulated plants were less sensitive to drought than nitrate-fed individuals; although as general growth was inhibited under drought stress in both experimental models. Results support the importance of nitrogen source in soybean responses to water stress. Difference in sensitivity of nitrate-fed and nitrogen-fixing plants towards water stress seems to be related not to nitrogen assimilation process itself, but to complex interactions with photorespiratory flux and stomatal conductance.  相似文献   

Tonsillar carcinoma in dogs: Treatment outcome and potential prognostic factors in 123 cases     

Elisabetta Treggiari  MacKenzie A. Pellin  Giorgio Romanelli  Gianluca Maresca  Irina Gramer  Andrew D. Yale  Evi Pecceu  Matteo Pignanelli  Juan Borrego  Katarzyna Purzycka  Davide Berlato 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):247-257

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Black locust (Robinia pseudoacacia L.) clonal seed orchards in Hungary     

Karoly Redei Zoltan Osvath-Bujtas Irina Veperdi 《中国林学(英文版)》2006,8(4):47-50

Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its importance is increasing in many countries. The main aim of the discussed new selection programme is to identify black locust clones with good performance and good form for setting up clonal seed orchards. As a result of selection programme 16 new black locust clones have been improved. In spring 2002 a black locust seed orchard was established with the newly selected clones. About 40% of the plants can be considered to belong to the height growth rate class 1 and 2. Hungary was the first country where micropropagated black locust planting material was used for seed orchard establishment.  相似文献   

Effect of κ/ß-Carrageenan from red alga Tichocarpus crinitus (Tichocarpaceae) on infection of detached tobacco leaves with tobacco mosaic virus     

Reunov  A.  Nagorskaya  Vera  Lapshina  Larissa  Yermak  Irina  Barabanova  Anna 《植物病害和植物保护杂志》2004,111(2):165-172

Journal of Plant Diseases and Protection - It is shown that κ/ß-carrageenan obtained from red marine alga Tichocarpus crinitus possesses antiviral activity against Tobacco mosaic virus...  相似文献   

Somatic Cell Nuclear Transfer in Horses     

Cesare Galli  Irina Lagutina  Roberto Duchi  Silvia Colleoni  Giovanna Lazzari 《Reproduction in domestic animals》2008,43(S2):331-337

The cloning of equids was achieved in 2003, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay was because of the limited development in the horse of more classical-assisted reproductive techniques required for successful cloning, such as oocyte maturation and in vitro embryo production. When these technologies were developed, the application of cloning also became possible and cloned horse offspring were obtained. This review summarizes the main technical procedures that are required for cloning equids and the present status of this technique. The first step is competent oocyte maturation, this is followed by oocyte enucleation and reconstruction, using either zona-enclosed or zona-free oocytes, by efficient activation to allow high cleavage rates and finally by a suitable in vitro embryo culture technique. Cloning of the first equid, a mule, was achieved using an in vivo -matured oocytes and immediate transfer of the reconstructed embryo, i.e. at the one cell stage, to the recipient oviduct. In contrast, the first horse offspring was obtained using a complete in vitro procedure from oocyte maturation to embryo culture to the blastocyst stage, followed by non-surgical transfer. Later studies on equine cloning report high efficiency relative to that for other species. Cloned equid offspring reported to date appear to be normal and those that have reached puberty have been confirmed to be fertile. In summary, horse cloning is now a reproducible technique that offers the opportunity to preserve valuable genetics and notably to generate copies of castrated champions and therefore, offspring from those champions that would be impossible to obtain otherwise.  相似文献   

In vitro susceptibilities to fluoroquinolones in current and archived Mycoplasma gallisepticum and Mycoplasma synoviae isolates from meat-type turkeys     

Gerchman I  Lysnyansky I  Perk S  Levisohn S 《Veterinary microbiology》2008,131(3-4):266-276

Monitoring of susceptibility to antibiotics in field isolates of pathogenic avian mycoplasmas is important for appropriate choice of treatment. Our study compared in vitro susceptibility to enrofloxacin and difloxacin in recent (2005-2006) isolates of Mycoplasma gallisepticum and Mycoplasma synoviae from meat-type turkey flocks with archived (1997-2003) isolates and reference strains. Comparison of minimal inhibitory concentration (MIC) values determined by microtest, agar dilution and commercial Etest showed good agreement, but underscored the need for standardized methods for testing. Notably, while the commercial Etest was convenient and accurate for determining MICs for enrofloxacin in the range 0.002-0.094mug/ml, the endpoint of inhibition for M. gallisepticum and M. synoviae strains with MIC values >/=1.0mug/ml could not be determined. A decrease in susceptibility to both fluoroquinolones was detected in archived strains but to a greater degree in recent isolates, most of which had MICs above the NCCLS susceptibility breakpoint for these antibiotics (相似文献   

Comparative Analysis of the Functional Properties of Films Based on Carrageenans,Chitosan, and Their Polyelectrolyte Complexes     

Aleksandra V. Volod&#x;ko  Viktoriya N. Davydova  Valentina A. Petrova  Dmitry P. Romanov  Evgeniya A. Pimenova  Irina M. Yermak 《Marine drugs》2021,19(12)

The influence of the structural features of carrageenan on the functional properties of the films was studied. The carrageenans and chitosan films, as well as three-layer films containing a polyelectrolyte complex (PEC) of the two, were prepared. The X-ray diffractograms of carrageenan films reflected its amorphous structure, whereas chitosan and three-layer films were characterized by strong reflection in the regions of 20° and 15° angles, respectively. The SEM of the cross-sectional morphology showed dense packing of the chitosan film, as well as the layer-by-layer structure of different densities for the PEC. Among the tested samples, κ/β-carrageenan and chitosan films showed the highest tensile strength and maximum elongation. Films containing the drug substance echinochrome were obtained. Mucoadhesive properties were assessed as the ability of the films to swell on the mucous tissue and their erosion after contact with the mucosa. All studied films exhibited mucoadhesive properties. All studied films exhibited mucoadhesive properties which depended on the carrageenans structure. Multilayer films are stronger than single-layer carrageenan films due to PEC formation. The resulting puncture strength of the obtained films was comparable to that of commercial samples described in the literature.  相似文献