Nematicidal effects of the entomopathogenic bacteria Bacillus thuringiensis in soil     

Irina N. Mozgovaya  Boris A. Byzov  Nikolai F. Ryabchenko  Nikolai D. Romanenko  Dmitri G. Zvyagintsev 《Pedobiologia》2002,46(6):558-572

  相似文献   

Isolation and multiresidue detection of macrolide endectocides present in animal matrices.   总被引：1，自引：0，他引：1  

Irina Rudik  Margie R Cummings  Robert H Poppenga 《Journal of veterinary diagnostic investigation》2002,14(4):295-302

A simple, rapid, and sensitive method for isolation and detection of macrolide endectocides (moxidectin, doramectin, selamectin, ivermectin, and eprinomectin) in animal sera and liver is described. Fortified sera or homogenized liver samples were treated with sodium chloride followed by organic solvent extraction. No additional steps were required prior to analysis. Separation of analytes and collection of mass information was achieved by liquid chromatography/mass spectrometry with positive atmospheric pressure chemical ionization set in selected ion monitoring mode with each sample analysis complete in 15 minutes. Presence of each compound was confirmed based on 2 separate extracted ion profiles. Detection of avermectins and moxidectin in a range of working standards was achieved at 10, 50, and 100 ppb. Quantitation of these compounds in fortified samples was based on standard calibration curves with R2 > 0.99. Detection limits of 10 ppb for ivermectin, moxidectin, and doramectin, 50 ppb for selamectin, and 100 ppb for eprinomectin were achieved in spiked sera. Recoveries of avermectins and moxidectin in 500 ppb fortified sera fell between 61 and 89% (+/-5.7-15.7). Analysis of fortified liver gave comparable results with recovery of selamectin of 83-91% +/- 18.3. A complete mass spectral fragmentation pattern of selamectin and affordable screening method for 6 macrolide endectocides are reported. Method comparison for salt treatment and solid-phase extraction of fortified samples is discussed.  相似文献   

Immunohistochemical evaluation of MMP-2 and TIMP-2 in canine mammary tumours: a survival study   总被引：1，自引：0，他引：1  

Santos A  Lopes C  Frias C  Amorim I  Vicente C  Gärtner F  Matos Ad 《Veterinary journal (London, England : 1997)》2011,(3):396-402

Canine mammary tumours (CMTs) are a very heterogeneous group of neoplasms with variable prognosis. Their aggressiveness is mainly due to their ability to invade locally and to metastasize. The degradation of extracellular matrix components is an important determinant of the invasive phenotype. The aims of this study were to analyse by immunohistochemistry and double immunofluorescence the expression of metalloproteinase 2 (MMP-2) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in eight normal canine mammary glands and 118 CMTs (24 benign, 94 malignant) and to investigate relationships with metastatic disease and survival.MMP-2 and TIMP-2 expression was higher in malignant tumours than in normal canine mammary tissue and benign tumours. The main difference between benign and malignant CMTs was the pattern of expression of both molecules: benign tumours presented TIMP-2 and MMP-2 immunoreactivity in the myoepithelial cells lining the basement membrane of tubuloalveolar structures, while malignant tumours showed mainly diffuse expression in neoplastic cells. In malignant tumours, increased TIMP-2 expression was significantly associated with the development of distant metastases, lower overall survival and lower disease-free survival. MMP-2 expression was not significantly associated to any of these parameters. These results suggest that the immunohistochemical expression of TIMP-2 is a useful prognostic factor in CMTs.  相似文献   

Research funding. Science foundations: a novelty in Russian science     

Dezhina I  Graham LR 《Science (New York, N.Y.)》2005,310(5755):1772-1773

  相似文献   

Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation   总被引：1，自引：0，他引：1  

Kratchmarova I  Blagoev B  Haack-Sorensen M  Kassem M  Mann M 《Science (New York, N.Y.)》2005,308(5727):1472-1477

Closely related signals often lead to very different cellular outcomes. We found that the differentiation of human mesenchymal stem cells into bone-forming cells is stimulated by epidermal growth factor (EGF) but not platelet-derived growth factor (PDGF). We used mass spectrometry-based proteomics to comprehensively compare proteins that were tyrosine phosphorylated in response to EGF and PDGF and their associated partners. More than 90% of these signaling proteins were used by both ligands, whereas the phosphatidylinositol 3-kinase (PI3K) pathway was exclusively activated by PDGF, implicating it as a possible control point. Indeed, chemical inhibition of PI3K in PDGF-stimulated cells removed the differential effect of the two growth factors, bestowing full differentiation effect onto PDGF. Thus, quantitative proteomics can directly compare entire signaling networks and discover critical differences capable of changing cell fate.  相似文献   

SILVER-INDUCED CHANGES IN THE NUTRIENT AND TRACE ELEMENT UPTAKE BY WHEAT AND AMOUNT OF THE RHIZOSPHERE PROTEINS     

Irina Shtangeeva  Salla Venäläinen  Helinä Hartikainen  Marina Surgik  Sergey Timofeev 《Journal of plant nutrition》2013,36(10):1497-1506

The information on biogeochemistry of silver (Ag) is scarce and mechanisms of Ag uptake by plants are still unclear. We studied the impact of silver sulfate (Ag₂SO₄) and silver nitrate (AgNO₃) on biomass of wheat seedlings, the rhizosphere proteins and uptake of nutrients and trace elements. Most part of Ag was accumulated in roots. Concentrations of Ag in leaves remained the same as in control plants. Both treatments (Ag₂SO₄ and AgNO₃) affected biomass of the seedlings. Plant biomass was decreased more after treatment with AgNO₃. Both Ag₂SO₄ and AgNO₃ reduced leaf concentrations of sodium and barium and root concentrations of calcium. Over time (three days), protein concentration in the rhizosphere of control plants and plants treated with AgNO₃ increased, whereas treatment with Ag₂SO₄ reduced concentration of the rhizosphere proteins. In soil treated with Ag₂SO₄ only low-molecular-weight proteins were found. In the control soil and in soil treated with AgNO₃ we also observed polydisperse fraction.  相似文献   

Clay mineral composition modifies decomposition and sequestration of organic carbon and nitrogen in fine soil fractions     

Cordula Vogel  Katja Heister  Franz Buegger  Irina Tanuwidjaja  Stephan Haug  Michael Schloter  Ingrid Kögel-Knabner 《Biology and Fertility of Soils》2015,51(4):427-442

  相似文献   

Atypical Reactive Center Kunitz-Type Inhibitor from the Sea Anemone Heteractis crispa     

I Gladkikh  M Monastyrnaya  E Leychenko  E Zelepuga  V Chausova  M Isaeva  S Anastyuk  Y Andreev  S Peigneur  J Tytgat  E Kozlovkaya 《Marine drugs》2012,10(7):1545-1565

The primary structure of a new Kunitz-type protease inhibitor InhVJ from the sea anemone Heteractis crispa (Radianthus macrodactylus) was determined by protein sequencing and cDNA cloning. InhVJ amino acid sequence was shown to share high sequence identity (up to 98%) with the other known Kunitz-type sea anemones sequences. It was determined that the P1 Thr at the reactive site resulted in a decrease of the K(i) of InhVJ to trypsin and α-chymotrypsin (7.38 × 10(-8) M and 9.93 × 10(-7) M, respectively). By structure modeling the functional importance of amino acids at the reactive site as well as at the weak contact site were determined. The significant role of Glu45 for the orientation and stabilization of the InhVJ-trypsin complex was elucidated. We can suggest that there has been an adaptive evolution of the P1 residue at the inhibitor reactive site providing specialization or functional diversification of the paralogs. The appearance of a key so-called P1 Thr residue instead of Lys might lead to refinement of inhibitor specificity in the direction of subfamilies of serine proteases. The absence of Kv channel and TRPV1-receptor modulation activity was confirmed by electrophysiological screening tests.  相似文献   

Spatial distribution of apricot roots in a semi-arid environment     

Cristian Paltineanu  Leinar Septar  Corina Gavat  Chitu Emil  Iancu Mihai  Alex Oprita  Cristina Moale  Lamureanu Gheorghe  Calciu Irina  Venera Mihaela Stroe 《Agroforestry Systems》2016,90(3):469-478

  相似文献   

Matrix metalloproteinase-9 expression in mammary gland tumors in dogs and its relationship with prognostic factors and patient outcome     

Santos AA  Lopes CC  Marques RM  Amorim IF  Gärtner MF  de Matos AJ 《American journal of veterinary research》2012,73(5):689-697

  相似文献