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21.
Analysing the agricultural sectors of Taiwan, Malaysia and Singapore, this paper examines the capability‐building process that encourages productivity and innovation. It describes and explains the origins and subsequent evolution of three forms of agricultural production system, each generating different farming capabilities and distinct forms of competitive advantages. The paper argues that Taiwan's rice‐oriented agricultural production system stimulates both productivity and innovation, helping Taiwanese farmers raise their income level and living standards. The active deployment of state institutions and a malleable labour force, evidenced in the Malaysian palm oil industry, is effective in raising farming productivity but not the ability to innovate. Singapore's aquaculture‐oriented agricultural production system is somewhat useful in stimulating productivity and innovation. Yet the city‐state's inherent lack of space and open international trade regime have circumscribed the potential of its aquaculture industry. It now relies on the regional ethnic Chinese business networks to expand the aquaculture industry's knowledge base and its industrial commons. The principles discussed in this paper provide policy lessons, or at least some initial guidance, for other developing economies aspiring to modernise their agricultural sector.  相似文献   
22.
The mammalian target of rapamycin (mTOR) protein kinase is a master growth promoter that nucleates two complexes, mTORC1 and mTORC2. Despite the diverse processes controlled by mTOR, few substrates are known. We defined the mTOR-regulated phosphoproteome by quantitative mass spectrometry and characterized the primary sequence motif specificity of mTOR using positional scanning peptide libraries. We found that the phosphorylation response to insulin is largely mTOR dependent and that mTOR exhibits a unique preference for proline, hydrophobic, and aromatic residues at the +1 position. The adaptor protein Grb10 was identified as an mTORC1 substrate that mediates the inhibition of phosphoinositide 3-kinase typical of cells lacking tuberous sclerosis complex 2 (TSC2), a tumor suppressor and negative regulator of mTORC1. Our work clarifies how mTORC1 inhibits growth factor signaling and opens new areas of investigation in mTOR biology.  相似文献   
23.
There has been rapid development in the marine foodfishlarviculture technology in Europe since the early eighties,especially in the flat fish, turbot and halibut, and the bass andbream species. The most significant improvements in the eightieswere the introduction of light control, artificial reproductiontechniques, appropriate water treatment and the use of rotifersand Artemia nauplii of specific sizes and in the late eightiesand early nineties the quality enhancement of live food organismsusing specific enrichment techniques. Present research is focusedon the implementation of several microbial techniques to improvethe hygiene of live prey and fish.Many of the modern larviculture techniques being used in marine foodfish could be adapted for application in the ornamental fish industry. For examples, research in the Onamental Fish Section, Primary Production Department, Singapore has demonstrated that the use of freshwater rotifers and Artemia nauplii would enable artificial rearing of Discus in the absence of the parent fish and improve the larval performance of Gouramis and Tetra larvae. The use of such small live food organisms is likely to facilitate breeding of new fish species with small larvae. Research has also indicated that the use of diets containing vitamin C and certain immuno-stimulants improved the stress resistance of guppy. Such techniques would have important application in the fish transport, an important aspect in the ornamental fish industry  相似文献   
24.
Milkfish (Chanos chanos) fry with an average weight of 40 mg per fish were stocked in 60-l wooden-glass aquaria filled with 30 l of filtered sea water with a salinity of 32–34 p.p.t. and a temperature of 25–28°C. They were fed diets containing 20, 30, 40, 50 and 60% protein and 2740 kcal of digestible energy per kg at a daily rate of 10% of the biomass for a period of 30 days. Fish which were fed the diet containing 40% protein had the highest weight gain which was significantly higher (P < 0.05) than those of fish receiving the lower dietary levels of protein. Slightly lower weight gains were obtained when fish were fed diets containing 50 and 60% protein. Although feed conversion values were not statistically different (P < 0.05) among all treatments, the value for the 40% protein diet (1.96) was the best. The mean survival rates were low for all treatments but was highest for the 40% protein diet. No significant difference (P < 0.05) was found in the survival rates of fish receiving different treatments. Results show that a dietary level of 40% protein was required by milkfish fry for maximum growth, efficient feed conversion and high survival rate.  相似文献   
25.
Landings in the blue crab, Portunus trituberculatus, fishery in Korean waters of the Yellow Sea have declined substantially from 11,000 t in the 1980s to 2,300 t in 2004. Blue crab habitat quality in the Yellow Sea has been degraded by anthropogenic activities including sand mining, land reclamation, and coastal pollution. Various traditional management measures have been implemented, including closed seasons during spawning and size limits, but these measures alone have been unsuccessful to conserve blue crab stocks. Consequently, a total allowable catch and a stock-rebuilding program using an ecosystem-based management approach were implemented in 2003 and 2006, respectively to rebuild blue crab stocks and restore habitats. This program involved assessment of both blue crab stock status and trammel-net fishery impacts at an ecosystem-level using an ecosystem-based fisheries assessment method ( [Zhang et al., 2009] and [Zhang et al., 2010]), which considered fishery data from catch and effort time-series, crab population biology, and ecosystem characteristics, including habitats and environmental conditions. Recent (2008) management status indices have shown significant positive change compared to conditions in 2000 with respect to sustainability of the stock and fishery and with regards to biodiversity and ecosystem habitat quality.  相似文献   
26.
Juvenile Nile tilapia were fed either a basal (control) diet (n = 6 aquaria) or a diet supplemented with 1 g/kg β‐glucan (n = 24 aquaria) for 4 wk. At the end of this period, fish receiving β‐glucan were continued on the same diet (n = 12 aquaria) or switched to the control diet (n = 12 aquaria) for 2 wk. After 6 wk, tilapia continuously fed the β‐glucan supplemented diets had improved weight gain and feed efficiency than those fed the control diet uninterrupted or switched from the β‐glucan diet to the control after 4 wk. Feeding tilapia β‐glucan for 4 wk and then switching to the basal diet for 2 wk caused a significant increase in the respiratory burst of polymorphonuclear lymphocytes (17.77 × 103 units/1000 white blood cells [WBC]) compared to catfish fed the control diet (13.50 × 103 units/1000 WBC) or the β‐glucan diet continuously (13.57 × 103 units/1000 WBC), but other immune parameters were unaffected. Tilapia were then challenged with Streptococcus iniae. The two groups were divided again (n = 6 aquaria) postchallenge and continued on the same diet or switched to the other diet (β‐glucan or control) for another 3 wk. No differences in survival to S. iniae infection occurred between dietary groups.  相似文献   
27.
ECOLOGY OF MYCOPLASMAS IN CLINICALLY HEALTHY CATS   总被引:1,自引:0,他引:1  
Mycoplasmas were isolated from various sites and organs of a series of 319 clinically healthy cats. They include M. felis, M. gateae, M. Arginini, A. laidlawii, feline ureaplasmas, M. pulmonis, M. arthritidis, and M. gallisepticum. In addition, there were 10 strains of mycoplasmas which could not be identified with the specific antiserums by growth inhibition tests. Antibody against M. felis was demonstrated by haemagglutination-inhibition and complement-fixation tests in cats which were over 6 months of age. However, no such antibody against M. felis was detected in animals which were less than 6 months old. No antibody against A. laidlawii, M. gateae, M. arginini and feline ureaplasmas was demonstrated by the same serological methods. The significance of these mycoplasmas in cats is described.  相似文献   
28.
Strongyloides venezuelensis (SVZ) infection was chronologically monitored in 85 Sprague-Dawley rats (SDR), which were orally inoculated with approximately 1,000 infective larvae. In order to describe the characteristics of migrating larvae (MLS) in various visceral organs (the liver, lung, cardiac blood, and small intestine), 5 SDR were sacrificed at 20 min, 45 min, 1 hr, 2 hr, 3 hr, 4 hr, 8 hr, 12 hr, 16 hr, 48 hr, 72 hr, 96 hr, 120 hr, 144 hr, 168 hr and 192 hr post inoculation (PI). MLS were recovered from the liver and blood 20 and 45 min PI and measured 788 +/- 26 microm and 846 +/- 40 microm in length, respectively. MLS were first observed in the lung tissue 45 min PI and measured 925 +/- 38 microm on the average. In the trachea, MLS measuring 849 +/- 75 microm appeared 3 to 96 hrs PI. Adult worms (AWS) measuring 1,926 +/- 521 microm to 2,956 +/- 159 microm in length were observed in the small intestine from 120 hr PI. The worms appeared to mature more than 168 hr PI and attained the average maximum length of 2,420 +/- 532 microm. At 3 hr PI focal hyperemic and necrotic lesions were evidently observed in the liver and lung, together with eosinophilic infiltration in the stomach, liver, and lung. The parasites were histologically detectable in the lung tissues but were very difficult to find in the liver and the epithelial layer of small intestine. These data demonstrate that SVZ parasites take 20 min to reach the liver via the stomach and only three hours to reach the trachea through the same route. The development from eggs to adults takes 168 hr in the SDR model.  相似文献   
29.
Polymorphisms and the antiviral property of porcine Mx1 protein   总被引:9,自引:0,他引:9  
We determined the cDNA sequences of the type I interferon-inducible proteins, pig Mx1 from PK(15) and LLC-PK1 cells, and compared the antiviral activities of both Mx proteins, including Mx1 polymorphisms against vesicular stomatitis virus (VSV). Mx1 cDNA derived from PK(15) cells had an 11 bp-deletion in the 3' end of the coding region, and was estimated to encode 8 amino acid substitutions and a 23 amino acid extension compared to that from LLC-PK1 cells. VSV replication was inhibited in the 3T3 cells expressing Mx1 mRNA after the cDNA was transfected. However, the efficiency of this inhibition was not different between the cells expressing Mx1 mRNA from both PK and LLC. These results indicate that pig Mx1 protein confers resistance to VSV.  相似文献   
30.
The objective of this study was to evaluate whether changes in NaCl concentration in a fertilization medium could improve normal fertilization and preimplantation development of bovine oocytes. In vitro matured bovine oocytes were inseminated with frozen-thawed semen for 18 hr in a Tyrode's medium with albumin, lactate and pyruvate (TALP), to which 114 (TALP-114), 96 (TALP-96) or 78 (TALP-78) mM NaCl was added. Presumptive zygotes were cultured for 192 hr in a modified TALP containing 90 mM NaCl, 1.5 mM glucose, 0.3% (w/v) BSA, minimal essential medium (MEM) essential and nonessential amino acids, and insulin-transferrin-selenium complex. Lower polyspermy rate was obtained by the insemination in TALP-96 (7.8 +/- 2.3%) than by the insemination in TALP-114 (25.6 +/- 1.4%), without decrease in male pronucleus (MPN) formation. Fertilization in TALP-78 also yielded decreased polyspermic fertilization (3.8 +/- 1.5%), but significant decrease in MPN formation was found (63.1 +/- 3.1%). In preimplantation development, more blastocysts developed from oocytes inseminated in TALP-96 (24.1 +/- 1.7%) than from oocytes inseminated in TALP-114 (16.8 +/- 1.4%). TALP-78, however, did not improve preimplantation development beyond the 8-cell stage compared with TALP-114. Mean cell number of blastocyst was higher when oocytes were fertilized in TALP-96 (137.0 +/- 4.5) than in TALP-114 (123.1 +/- 5.1) and in TALP-78 (102.3 +/- 4.5). These results demonstrate that insemination of bovine oocytes in a TALP with decreased NaCl concentration (96 mM) improves blastocyst formation and embryo viability. Decrease in NaCl concentration below 96 mM, however, may delay or inhibit MPN formation, and inhibits subsequent development in vitro.  相似文献   
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