Evaluation of a radioimmunoassay for type III procollagen peptide in the dog     

Dimski DS  Brooks CL  Johnson SE 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(2):40-44

Type III procollagen peptide (P-3-P) is a serum marker for hepatic fibrosis in humans. The utility of a commercially available radioimmunoassay for P-3-P was evaluated in the dog. The specificity of the assay was assessed by polyacrylamide gel electrophoresis (PAGE) of canine serum and purified bovine P-3-P, followed by Western immunoblotting with rabbit aniti-P-3-P serum. The sensitivity was assessed by performing the radioimmunoassay on dilutions of sera from 22 dogs. Polyacrylamide gel electrophoresis of purified bovine P-3-P and sera from two dogs suspected of having elevated P-3-P concentrations revealed no homologous bands of staining. Western immunoblotting showed marked cross-reactivity of the high antisera concentrations with several components of the serum proteins, but none corresponding to the purified P-3-P. All tested sera from dogs had minimal competitive binding with radiolabeled P-3-P in the radioimmunoassay. Dilution curves of dog sera did not parallel either the standard curve or the dilution curve of a known test human serum. There were no statistically different P-3-P concentrations in any of the groups of dogs studied. It was concluded that currently available radioimmunoassay kits for the measurement of P-3-P in the human are not applicable in the dog. Seemingly, the structure or metabolism of canine P-3-P may vary significantly from that of the bovine or human, limiting the sensitivity and specificity of this assay in the dog.  相似文献   

Field studies with convalescent serum and infectious bursal disease vaccine to control turkey coryza     

D C Johnson  P D Lukert  R K Page 《Avian diseases》1980,24(2):386-392

Convalescent serum given to 1-day-old poults delayed clinical signs of turkey coryza by several days and reduced mortality on infected farms. Turkey breeders immunized with cell-culture-adapted infectious bursal disease virus (IBDV) or turkey infectious bursal disease virus (TIBDV) had a marked increase in virus-neutralization (VN) antibody titers. The VN antibody titer was significantly higher in progeny poults than in poults from unimmunized breeders. Clinical turkey coryza and mortality was considerably less in poults from IBD- or TIBD-vaccinated breeders than in control poults. They also responded more favorably to hemorrhagic enteritis and fowl cholera vaccination.  相似文献   

Information dissemination: whatever happened to the weinberg report?     

Johnson IY 《Science (New York, N.Y.)》1978,200(4337):6

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Maternal immunity to infectious bovine rhinotracheitis and bovine viral diarrhea viruses: duration and effect on vaccination in young calves.     

J S Brar  D W Johnson  C C Muscoplat  R E Shope  J C Meiske 《American journal of veterinary research》1978,39(2):241-244

The immune response to modified live-virus bovine viral diarrhea (BVD) vaccine and infectious bovine rhinotracheitis (IBR) vaccine was examined in calves that had received passive maternal antibodies to these viruses. Blood serum samples from vaccinated and control (nonvaccinated) calves were examined for more than 1 year to determine the rate of decline of passive anti-BVD and anti-IBR antibodies and the effect that vaccination had on these antibody titers. The control calves lost their antibodies to BVD and IBR viruses at the rate of one half their remaining antibody titer every 21 days. Calves serologically responded to BVD vaccine at a time when maternal antibody titers remained between 1:96 and 1:20. However, animals did not seroconvert to the IBR vaccine until maternal antibodies had decreased and become undetectable. Evidence is presented to show that although passive immunity will inhibit IBR vaccination, priming for a secondary response will occur so that on subsequent vaccination, at a time when maternal antibodies have disappeared, the animals will respond anamnestically to IBR vaccination.  相似文献   

The importance of adrenocortical steroids in the pathogenesis of ketosis in diabetic guinea pigs (Caviae porcellus)     

F M Johnson 《The Cornell veterinarian》1978,68(2):150-160

The roles of adrenocortical steroids in the pathogenesis of diabetic ketosis were investigated in guinea pigs. Groups of normal and Streptozotocin-induced diabetic animals were either given saline as a control, methylprednisilone as an exogenous steroid, or adrenocorticotrophin to stimulate endogenous steroid production. Plasma and urine ketone determinations were made daily for six days at which time animals were necropsied. The qualitative procedure used failed to detect ketonemia, and ketonuria was only found in diabetic guinea pigs treated with steroids. The results support the hypothesis that the absence of ketosis in spontaneously diabetic guinea pigs may be the result of a relative glucocorticoid insufficiency secondary to fatty degeneration of the adrenal glands, a common lesion in spontaneously diabetic guinea pigs.  相似文献   

Replacement of maize by triticale in broiler diets     

D. R. Rao  W. M. Johnson  G. R. Sunki 《British poultry science》1976,17(3):269-274

1. The nitrogen‐corrected metabolisable energy contents of the triticale varieties Multi Graze, Graze Grain 70, 204 and 131 were 12.28, 12.69, 12.37 and 12.03 MJ/kg respectively.

2. Increasing proportions (0, 25, 50 or 75%) of the maize of a diet were replaced by triticale 131 to provide diets of equal energy and nitrogen contents for broilers. These diets resulted in weight gains of 1.775, 1.782, 1.713 and 1.808 kg respectively at 8 weeks and there were no significant differences among efficiencies of food conversions.

3. Dressed carcass weights as percentages of body weight were 74.38, 75.01, 73.98 and 74.50 respectively and did not differ significantly.

4. Triticale in the diet did not seem to influence the relative weights of either liver or gizzard.

5. The flavour of the meat seemed to become less acceptable as the triticale in the diet increased but there were no significant differences in tenderness and juiciness of the meat caused by the treatments.  相似文献   

Carbon storage by urban tree cultivars, in roots and above-ground     

Andra D. Johnson  Henry D. Gerhold 《Urban Forestry & Urban Greening》2003,2(2):65-72

Urban trees can favorably affect factors underlying global warming by storing carbon and by reducing energy needs for cooling and heating buildings. To estimate carbon stored in roots and above-ground portions of trees, data was collected consisting of whole tree sampling of Amelanchier, Malus, Pyrus, and Syringa cultivars. Roots were excavated using an Air-Spade^™. Regression analysis resulted in two equations for predicting total carbon storage based on height and diameter of trees up to 20 cm dbh: Y = 0.05836 (dbh²) for root carbon storage, and Y = 0.0305 (dbh² × h)^0.9499 for above-ground carbon storage, explaining 97% and 96% of the variation, respectively. Average carbon stored in roots of various cultivars ranged from 0.3 to 1.0 kg for smaller trees, those 3.8 to 6.4 cm dbh, to more than 10.4 kg for trees 14.0 cm to 19.7 cm dbh. Average total carbon stored by cultivars ranged from 1.7 to 3.6 kg for trees less than 6.4 cm dbh to 54.5 kg for trees larger than 14.0 cm. The data from these equations apply mainly to trees in nurseries and recently transplanted trees. Comparisons showed that above-ground estimates from previous studies using a sampling technique overestimated values obtained from actual above-ground weights.  相似文献   

Genetic analyses of resistance of the wheat differential cultivars Carstens V and Spaldings Prolific to two races of Puccinia striiformis     

A. Calonnec †‡  R. Johnson §  C. de Vallavieille-Pope 《Plant pathology》2002,51(6):777-786

Genetic analysis of resistance of wheat seedlings to two races of Puccinia striiformis was conducted on F₁, F₂ and F₃ generations from crosses Carstens V (CV) × Lee, Spaldings Prolific (SPA) × Lee and CV × SPA. F₂ generations from crosses of CV and SPA with Strubes Dickkopf (SD) were also studied. The plants were classified into six resistance classes and analysed by factorial correspondence analysis and nonhierarchical classification. The two P. striiformis isolates tested were a French isolate of race 43E138 and a Lebanese isolate of race 2E16, selected for the differences in their virulence spectra for the common differential cultivars Strubes Dickkopf and Nord Desprez. Resistance of CV and SPA was recessive and dominant to races 43E138 and 2E16, respectively. CV possessed three or four resistance genes, one of them being expressed with both races. Two genes of CV had a cumulative effect for resistance to 43E138 and two or three gave dominant resistance to 2E16. SPA had three resistance genes, all of which gave resistance to 2E16 and two of which also gave resistance to 43E138. SPA had one gene in common with CV for resistance to both races. Furthermore, the gene for resistance to race 2E16 in CV and SPA was allelic with a gene in SD, and was probably Yr25 .  相似文献   

Quantifying patch distribution at multiple spatial scales: Applications to wildlife-habitat models     

Johnson  Chris J.  Boyce  Mark S.  Mulders  Robert  Gunn  Anne  Gau  Rob J.  Cluff  H. Dean  Case  Ray L. 《Landscape Ecology》2004,19(8):869-882

Multiscale analyses are widely employed for wildlife-habitat studies. In most cases, however, each scale is considered discrete and little emphasis is placed on incorporating or measuring the responses of wildlife to resources across multiple scales. We modeled the responses of three Arctic wildlife species to vegetative resources distributed at two spatial scales: patches and collections of patches aggregated across a regional area. We defined a patch as a single or homogeneous collection of pixels representing 1 of 10 unique vegetation types. We employed a spatial pattern technique, three-term local quadrat variance, to quantify the distribution of patches at a larger regional scale. We used the distance at which the variance for each of 10 vegetation types peaked to define a moving window for calculating the density of patches. When measures of vegetation patch and density were applied to resource selection functions, the most parsimonious models for wolves and grizzly bears included covariates recorded at both scales. Seasonal resource selection by caribou was best described using a model consisting of only regional scale covariates. Our results suggest that for some species and environments simple patch-scale models may not capture the full range of spatial variation in resources to which wildlife may respond. For mobile animals that range across heterogeneous areas we recommend selection models that integrate resources occurring at a number of spatial scales. Patch density is a simple technique for representing such higher-order spatial patterns.  相似文献   

Catheter‐associated venous air embolism in hospitalized horses: 32 cases          下载免费PDF全文

Nicholas J. Parkinson  Harold C. McKenzie  Michelle H. Barton  Jennifer L. Davis  Bettina Dunkel  Amy L. Johnson  Elizabeth S. MacDonald 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(2):805-814

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