Cutaneous hybrid cyst in a sprague-dawley rat     

K Yasuno  H Sakashita  S Araki  R Kobayashi  Y Sakurada  M Shirota  J Kamiie  K Shirota 《Journal of toxicologic pathology》2012,25(2):175-178

This report describes a spontaneous hybrid cyst in a Sprague-Dawley (SD) rat. A 52-week-old, male SD rat had a cutaneous cyst on the left mystacial pad. Histologically, the cyst wall showed infundibular differentiation with keratohyalin granules in the granular layer and matrical differentiation comprising basaloid epithelial cells with trichohyalin granules. The cyst cavity was filled with lamellar, flaky keratin and aggregates of shadow cells. Immunohistochemically, the infundibular-type epithelium was positive for cytokeratin (CK) AE1/AE3, CK KL1 and CK14 but negative for CK15, whereas the matrical-type epithelium was negative for all four CK isoforms examined. These immunohistochemical properties of the infundibular- and matrical-type epithelia were similar to those of the infundibulum and inferior segment of normal hair follicles, respectively. Based on these findings, the cyst was diagnosed as a hybrid cyst, comprising more than one type of cyst arising from various parts of the pilosebaceous unit.  相似文献   

Characterization of canine caspase-3   总被引：1，自引：0，他引：1  

Sano J  Oguma K  Kano R  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):563-567

The canine caspase-3 gene was cloned and sequenced. The canine caspase-3 cDNA clone was 1473 base pairs in length and encoded 277 amino acids. The predicted amino acid sequence showed 88.4%, 88.0%, 85.9%, 65.9% and 60.6% homology with that of human, pig, mouse, chicken and zebra fish caspase-3, respectively. The caspase-3 mRNA was confirmed to express in skin, lymph node, bone marrow, small intestine and lung from a healthy dog by RT-PCR analysis.  相似文献   

Characterization of methicillin-resistant Staphylococcus aureus isolated from retail raw chicken meat in Japan     

Kitai S  Shimizu A  Kawano J  Sato E  Nakano C  Uji T  Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):107-110

Two isolates of mecA-positive methicillin-resistant Staphylococcus aureus (MRSA) from retail raw chicken meat were characterized by phenotypic and genotypic methods. One isolate showed the human biovar, coagulase type III, phage group I * III, the lack of production of enterotoxins and TSST-1, and resistance to PCG/ABPC/EM/GM/KM. The other isolate showed the human biovar, coagulase type III, phage group III, production of enterotoxin C and TSST-1, and resistance to PCG/ABPC/CEZ. The biotyping results indicate that the two isolates showed characteristics of human S. aureus. They also harbored SCCmec type IV, which has prevalently been found in community-acquired MRSA isolates. This paper is the first publication regarding MRSA isolates from raw chicken meat in Japan.  相似文献   

DNA fragmentation in Sekiguchi lesion mutants of rice infected with Magnaporthe grisea     

Makoto Ueno  Junichi Kihara  Yuichi Honda  Jun Isota  Sakae Arase 《Journal of General Plant Pathology》2004,70(6):321-328

Light-dependent activation of the tryptamine pathway in Sekiguchi lesion (sl) mutants (Sekiguchi-asahi, Sekiguchi-koshihikari, and Sekiguchi-sasanishiki) inoculated with Magnaporthe grisea, was demonstrated by a significant increase in tryptophan decarboxylase and monoamine oxidase activities and tryptamine accumulation. Terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL) staining and gel analysis indicated DNA fragmentation in cells of leaf tissues with Sekiguchi lesions of the three sl mutants. Furthermore, increased DNase activity was also light-dependent in the sl mutants after inoculation with M. grisea. DNA fragmentation was inhibited in leaves when Sekiguchi lesion formation was suppressed by cycloheximide and heat shock pretreatments. These data suggest that Sekiguchi lesion formation in the sl mutants is an apoptosis-like response and that its response is induced by light-dependent activation of the tryptamine pathway, which is responsible for light-enhanced resistance.The nucleotide sequence data reported are available in the DDBJ/GenBank/EMBL databases under accession number AB162137 for TDC  相似文献   

Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2alpha production     

Acosta TJ  Yoshioka S  Komiyama J  Lee SH  Grazul-Bilska AT  Skarzynski DJ  Okuda K 《The Journal of reproduction and development》2007,53(3):473-480

To establish a storage system for isolated bovine luteal endothelial cells (LECs), we investigated the basal and tumor necrosis factor (TNF) alpha-stimulated production of endothelin-1 (ET-1) and prostaglandin (PG) F2alpha in unfrozen and frozen-thawed LECs until passage 10. LECs were obtained from developing corpora lutea (CL; days 5-7 of the estrous cycle) using enzymatic digestion and magnetic beads coated with lectin BS-1. The LECs were frozen at -80 C or further cultured and/or passaged until passage 10 in DMEM/Ham's F-12 supplemented with 10% calf serum. The hormonal productions of unfrozen and frozen/thawed LECs were compared through passages 2-10. When both the unfrozen and frozen/thawed cells reached confluence, the culture medium was replaced with fresh medium containing 0.1% bovine serum albumin (BSA), and the cells were incubated with TNFalpha (50 ng/ml) for 12 h. The basal productions of ET-1 and PGF2alpha by the unfrozen and frozen/thawed LECs were similar at passage 2. The basal production of PGF2alpha by LECs was not altered by passage and storage at -80 C, whereas the basal production of ET-1 decreased from passage 2 and 3 to passage 4 in the unfrozen LECs and from passage 2 to passage 3 in the frozen/thawed LECs. However, production of ET-1 by the unfrozen and frozen/thawed LECs was similar between passages 4-10 and passages 3-10, respectively. Exposure of LECs to TNFalpha increased (P<0.05) ET-1 and PGF2alpha production by the unfrozen and frozen-thawed LECs in all passages examined. Thus, LECs obtained from developing CLs and stored until passage 10 can be used for study of the physiology of LECs in vitro.  相似文献   

Pathological features of proteinuric nephropathy resembling Alport syndrome in a young Pyrenean Mountain dog     

Go SUGAHARA  Ichiro NAITO  Yuichi MIYAGAWA  Takaaki KOMIYAMA  Naoyuki TAKEMURA  Ryosuke KOBAYASHI  Takayuki MINESHIGE  Junichi KAMIIE  Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1175-1178

The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with renal disorder and severe proteinuria was examined. Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM. Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli. These results suggested that the glomerular disease of the present case might be consistent with canine hereditary nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen, and indicated possible contribution of podocyte injury to severe proteinuria in this case.  相似文献   

Inhibition of hepatitis C virus replication and viral helicase by ethyl acetate extract of the marine feather star Alloeocomatella polycladia     

Yamashita A  Salam KA  Furuta A  Matsuda Y  Fujita O  Tani H  Fujita Y  Fujimoto Y  Ikeda M  Kato N  Sakamoto N  Maekawa S  Enomoto N  Nakakoshi M  Tsubuki M  Sekiguchi Y  Tsuneda S  Akimitsu N  Noda N  Tanaka J  Moriishi K 《Marine drugs》2012,10(4):744-761

Hepatitis C virus (HCV) is a causative agent of acute and chronic hepatitis, leading to the development of hepatic cirrhosis and hepatocellular carcinoma. We prepared extracts from 61 marine organisms and screened them by an in vitro fluorescence assay targeting the viral helicase (NS3), which plays an important role in HCV replication, to identify effective candidates for anti-HCV agents. An ethyl acetate-soluble fraction of the feather star Alloeocomatella polycladia exhibited the strongest inhibition of NS3 helicase activity, with an IC(50) of 11.7 μg/mL. The extract of A. polycladia inhibited interaction between NS3 and RNA but not ATPase of NS3. Furthermore, the replication of the replicons derived from three HCV strains of genotype 1b in cultured cells was suppressed by the extract with an EC(50) value of 23 to 44 μg/mL, which is similar to the IC(50) value of the NS3 helicase assay. The extract did not induce interferon or inhibit cell growth. These results suggest that the unknown compound(s) included in A. polycladia can inhibit HCV replication by suppressing the helicase activity of HCV NS3. This study may present a new approach toward the development of a novel therapy for chronic hepatitis C.  相似文献   

Behavioural characteristics of loggerhead turtles (Caretta caretta) in a submerged bag net of a setnet observed in a bycatch simulation and the development of a turtle releasing device     

Maika Shiozawa  Daisuke Shiode  Junichi Okuyama  Fuxiang Hu  Tadashi Tokai 《水产资源保护:海洋与淡水生态系统》2019,29(12):2107-2115

小麦薄层干燥特性研究   总被引：5，自引：1，他引：5  

李长友  上出顺一 《华南农业大学学报》1995,16(3):1-5

在确保送温温度恒定的条件下，考察了小麦的薄层干燥特性，小麦的降速干燥过程，可区分为２段降速干燥；降速干燥速率主要受送风温度的支配，受空气的温度的影响相对较小。  相似文献   

Congenital malformations of the external and middle ear accompanied by temporal bone anomaly in a calf     

Naoyuki AIHARA  Anna MOMOKI  Nanase HATTORI  Hironobu MURAKAMI  Motoharu OISHI  Yoshiaki GOTO  Reiichiro SATO  Makoto NAGAI  Kazutaka YAMADA  Junichi KAMIIE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):302

A 7-month-old female Holstein calf presented with bilateral microtia and absent external acoustic meatus. The real-time polymerase chain reaction test was negative for bovine viral diarrhea virus and bovine leukemia virus. The calf’s dam had a normal reproductive history. Computed tomography confirmed bilateral atresia of external auditory canals, aplasia of tympanic cavities and the ossicular chain, and temporomandibular joint abnormality. Necropsy revealed a severe malformation of the temporal bone. In the tympanic region, the external acoustic pore, tympanic bulla, and muscular process were absent bilaterally. The bilateral inner ear structure was normal. Based on these findings, we diagnosed the present case as congenital malformations of the external and middle ear accompanied by temporal bone anomaly.  相似文献