Anti-Inflammatory Cembranoids from a Formosa Soft Coral Sarcophyton cherbonnieri     

Chia-Chi Peng  Chiung-Yao Huang  Atallah F. Ahmed  Tsong-Long Hwang  Jyh-Horng Sheu 《Marine drugs》2020,18(11)

The present investigation on chemical constituents of the soft coral Sarcophyton cherbonnieri resulted in the isolation of seven new cembranoids, cherbonolides F–L (1–7). The chemical structures of 1–7 were determined by spectroscopic methods, including infrared, one- and two-dimensional (1D and 2D) NMR (COSY, HSQC, HMBC, and NOESY), MS experiments, and a chemical reduction of hydroperoxide by triphenylphosphine. The anti-inflammatory activities of 1–7 against neutrophil proinflammatory responses were evaluated by measuring their inhibitory ability toward N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLF/CB)-induced superoxide anion generation and elastase release in primary human neutrophils. The results showed that all isolates exhibited moderate activities, while cherbonolide G (2) and cherbonolide H (3) displayed a more active effect than others on the inhibition of elastase release (48.2% ± 6.2%) and superoxide anion generation (44.5% ± 4.6%) at 30 µM, respectively.  相似文献   

Pinnisterols A–C,New 9,11-Secosterols from a Gorgonian Pinnigorgia sp.     

Yu-Chia Chang  Liang-Mou Kuo  Tsong-Long Hwang  Jessica Yeh  Zhi-Hong Wen  Lee-Shing Fang  Yang-Chang Wu  Chan-Shing Lin  Jyh-Horng Sheu  Ping-Jyun Sung 《Marine drugs》2016,14(1)

Three new 9,11-secosterols, pinnisterols A–C (1–3), were isolated from a gorgonian coral Pinnigorgia sp., collected off the waters of Taiwan. The structures of these compounds were elucidated on the basis of spectroscopic methods. The new sterols 1 and 3 displayed significant inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils, and sterol 1 was found to show moderate cytotoxicity in hepatic stellate cells (HSCs).  相似文献   

Briarenolides K and L,New Anti-Inflammatory Briarane Diterpenoids from an Octocoral Briareum sp. (Briareidae)     

Yin-Di Su  Tzu-Rong Su  Zhi-Hong Wen  Tsong-Long Hwang  Lee-Shing Fang  Jih-Jung Chen  Yang-Chang Wu  Jyh-Horng Sheu  Ping-Jyun Sung 《Marine drugs》2015,13(2):1037-1050

Two new briarane-type diterpenoids, briarenolides K (1) and L (2), were isolated from an octocoral identified as Briareum sp. The structures of new briaranes 1 and 2 were elucidated by spectroscopic methods. In the in vitro anti-inflammatory effects test, briaranes 1 and 2 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells.  相似文献   

Briarenolides F and G, new briarane diterpenoids from a Briareum sp. octocoral     

PH Hong  YD Su  JH Su  YH Chen  TL Hwang  CF Weng  CH Lee  ZH Wen  JH Sheu  NC Lin  YH Kuo  PJ Sung 《Marine drugs》2012,10(5):1156-1168

Two new briarane diterpenoids, briarenolides, F (1) and G (2), were isolated from an octocoral identified as Briareum sp. The structures of briaranes 1 and 2 were established by spectroscopic methods and by comparison of the spectroscopic data with those of known briarane analogues. Briarenolide F was proven to be the first 6-hydroperoxybriarane derivative and this compound displayed a significant inhibitory effect on the generation of superoxide anion by human neutrophils.  相似文献   

Natural Product Chemistry of Gorgonian Corals of the Family Plexauridae Distributed in the Indo-Pacific Ocean     

Li-Hsueh Wang  Jyh-Horng Sheu  Shih-Yao Kao  Jui-Hsin Su  Yung-Husan Chen  Yu-Hsin Chen  Yin-Di Su  Yu-Chia Chang  Lee-Shing Fang  Wei-Hsien Wang  Yang-Chang Wu  Ping-Jyun Sung 《Marine drugs》2012,10(11):2415-2434

The structures, names, bioactivities and references of 105 natural products obtained from gorgonian corals belonging to the family Plexauridae with an Indo-Pacific distribution are described in this review. All compounds mentioned in this review were obtained from gorgonian corals belonging to the genera Astrogorgia, Bebryce, Echinomuricea, Euplexaura and Menella.  相似文献   

Arsenic accumulation by rice grown in soil treated with roxarsone     

Chen‐Wuing Liu  Ching‐Chieh Lin  Cheng‐Shin Jang  Guey‐Rong Sheu  Lo Tsui 《植物养料与土壤学杂志》2009,172(4):550-556

Poultry litter is widely used as a fertilizer for lowland rice in Taiwan and China. However, the organic‐arsenic compound roxarsone (additive of poultry feed) in poultry litter can be absorbed by the plants and the resulting arsenic (As) contamination may pose a serious threat to human health. This study used various amounts of poultry litter contaminated with roxarsone in pot experiments to evaluate the effect of roxarsone on rice agronomic parameters and the bioaccumulation of total and inorganic As in rice‐plant tissues. Rice‐grain yield decreased significantly with increasing As content of the soil, and the critical threshold that killed rice was 200 mg roxarsone (kg soil)^–1. The As concentrations in root, straw, leaf, husk, and grain increased with increasing soil As (p < 1%). At 100 mg roxarsone per kg of soil, the As concentration in the rice grain exceeded the statutory permissible limit of 1.0 mg As (kg dry weight)^–1 and at 25 mg roxarsone (kg soil)^–1, the inorganic As concentrations in grains exceeded the statutory limit of 0.15 mg of inorganic As kg^–1 in China. For all treatments, the As concentrations in various plant tissues at maturity follow the order: root > stem > leaf > husk > grain. Arsenite was the predominant species in root, straw, and grain, while arsenate was the predominant species in leaf and husk. No significant difference existed between the amounts of arsenite and arsenate when various amounts of poultry litter were applied. This result illustrates that large amounts of added roxarsone are not only toxic to rice but also accumulate in grains in the inorganic As forms, potentially posing a threat to human health via the food chain.  相似文献   

Red soil chemistry and mineralogy reflect uniform weathering environments in fluvial sediments, Taiwan     

Tsung Ming Tsao  Yue Ming Chen  Hwo Shuenn Sheu  Shung Yao Zhuang  Ping Hua Shao  Hua Wen Chen  Kai Shuan Shea  Ming Kuang Wang  Yen Horng Shau  Kai Yin Chiang 《Journal of Soils and Sediments》2012,12(7):1054-1065

Purpose

Information on the physicochemical properties, mineral species and micromorphology of lateritic soils and gravel soil layers in paleo-environmental soil profile is severely lacking. Red soil profile of the Taoyuan terrace was employed to demonstrate its different extents of lateritic weathering. The objectives of this study were to compare the physicochemical properties of lateritic soils and gravel soil layers and identify using conventional and synchrotron X-ray diffraction (XRD) analyses mineral species in nanoparticles separated by automated ultrafiltration device (AUD) apparatus.

Materials and methods

Soil samples were collected from paleo-environmental lateritic soils. Soil samples were examined using elemental analysis, conventional and synchrotron XRD analyses, high gradient magnetic separation, separation and collection of nanoparticles by AUD apparatus, and transmission electron microscopy (TEM).

Results and discussion

The soil pH, redness index, quantities of free Al- and Fe-oxides (Al_d and Fe_d), and clay content of lateritic soils are higher than those of gravel soil layers. Illite, kaolinite, gibbsite, quartz, goethite, and hematite were identified in clay fractions and nanoparticles by conventional and synchrotron XRD analyses. TEM images show presence of hematite nanoparticles on the surface coating of kaolinite nanoparticles and aggregated hematite nanoparticles overlapping the edge of a kaolinite flake in a size range of 4?C7?nm. Synchrotron XRD techniques are more straightforward and powerful than conventional XRD with random powder methods for identifying nanoparticles in red soils, particularly for illite, kaolinite, goethite, and hematite nanoparticles. According to chemical compositions of clay fractions and red soil features in the Taoyuan terrace, these red soils can be taken as lateritic red earths or red earths.

Conclusions

This work suggests that physicochemical properties, mineral species, and micromorphology of red soil at all depths can shed light on the extent of paleo-environmental lateritic weathering.  相似文献   

Bioactive eunicellin-based diterpenoids from the soft coral Cladiella krempfi     

Tai CJ  Su JH  Huang MS  Wen ZH  Dai CF  Sheu JH 《Marine drugs》2011,9(10):2036-2045

Four new eunicellin-based diterpenoids, krempfielins A-D (1-4), along with two known compounds (5 and 6) have been isolated from a soft coral Cladiella krempfi. The structures of the new metabolites were elucidated by extensive spectroscopic analysis and by comparison with spectroscopic data of related known compounds. Compounds 5 and 6 were shown to exhibit cytotoxicity against a limited panel of cancer cell lines. Furthermore, compounds 2, 3, 5 and 6 were shown to exert significant in vitro anti-inflammatory activity against LPS-stimulated RAW264.7 macrophage cells.  相似文献   

nardosinane-type sesquiterpenoids from the formosan soft coral Paralemnalia thyrsoides     

Huang CY  Su JH  Chen BW  Wen ZH  Hsu CH  Dai CF  Sheu JH  Sung PJ 《Marine drugs》2011,9(9):1543-1553

Five new nardosinane-type sesquiterpenoids, paralemnolins Q-U (1-5), along with three known compounds (6-8), were isolated from the Formosan soft coral Paralemnalia thyrsoides. The structures of new metabolites were elucidated on the basis of extensive spectroscopic methods, and the absolute configuration of 1 was determined by the application of Mosher's method on 1. Among these metabolites, 1 and 3 are rarely found nardosinane-type sesquiterpenoids, possessing novel polycyclic structures. Compounds 1, 3, 6 and 7 were found to possess neuroprotective activity.  相似文献   

Immunoadjuvant activities of a recombinant chicken IL-12 in chickens vaccinated with Newcastle disease virus recombinant HN protein   总被引：1，自引：0，他引：1  

Su BS  Yin HS  Chiu HH  Hung LH  Huang JP  Shien JH  Lee LH 《Veterinary microbiology》2011,151(3-4):220-228

Recombinant fowlpox virus (rFPV/HN) expressing Newcastle disease virus (NDV) HN gene and rFPV/HN/chIL-12 co-expressing chicken IL-12 (chIL-12) and HN (rHN/chIL-12) genes have been characterized. rHN/chIL-12 or rchIL-12, expressed by our previous construct rFPV/chIL-12, co-administered with rHN was assessed for adjuvant activities of chIL-12. Chickens were vaccinated with various amounts of rHN/chIL-12 mixed with mineral oil (MO), intramuscularly. Levels of hemagglutination-inhibition (HI) antibody production depended on the concentration of the injected rHN or rHN/chIL-12. The lower HI antibody titers were obtained in chicken groups rHN/chIL-12/7-rHN/chIL-12/9, receiving 60ng rHN/8ng chIL-12 with MO, 30ng rHN/4ng chIL-12 with MO or 15ng rHN/2ng chIL-12 with MO, respectively, compared to those in chicken groups rHN/7-rHN/9, receiving rHN with MO alone. However, chickens in group rHN/chIL-12/7 or rHN/chIL-12/8 and rHN with MO alone showed the same effective protection. Chicken group rHN/chIL-12/9 was even more protective than that in group rHN/9. When rchIL-12 was co-injected with 15ng rHN plus MO, chickens produced low levels of HI antibody titers; while higher levels of IFN-γ production and an effective protection rate (83%) were obtained. On the other hand, low levels of IFN-γ production and low protection response (50%) were obtained in chickens injected with rHN with MO alone. Taken together, when the concentration of rHN decreased to certain levels, rchIL-12 reduced HI antibody production. The increase in the induction of IFN-γ production might suggest the enhancement of the cell-mediated immunity which conferred the protection from the NDV challenge.  相似文献