Lobocrassins A-E: new cembrane-type diterpenoids from the soft coral Lobophytum crassum     

Kao CY  Su JH  Lu MC  Hwang TL  Wang WH  Chen JJ  Sheu JH  Kuo YH  Weng CF  Fang LS  Wen ZH  Sung PJ 《Marine drugs》2011,9(8):1319-1331

Five new cembrane-type diterpenoids, lobocrassins A-E (1-5), were isolated from the soft coral Lobophytum crassum. The structures of cembranes 1-5 were established by spectroscopic and chemical methods and by comparison of the spectral data with those of known cembrane analogues. Lobocrassin A (1) is the first cembranoid possessing an α-chloromethyl-α-hydroxy-γ-lactone functionality and is the first chlorinated cembranoid from soft corals belonging to the genus Lobophytum. Lobocrassins B (2) and C (3) were found to be the stereoisomers of the known cembranes, 14-deoxycrassin (6) and pseudoplexaurol (7), respectively. Lobocrassin B (2) exhibited modest cytotoxicity toward K562, CCRF-CEM, Molt4, and HepG2 tumor cells and displayed significant inhibitory effects on the generation of superoxide anion and the release of elastase by human neutrophils.  相似文献   

Bioactive cembranoids from the dongsha atoll soft coral Sarcophyton crassocaule     

Lin WY  Lu Y  Su JH  Wen ZH  Dai CF  Kuo YH  Sheu JH 《Marine drugs》2011,9(6):994-1006

Seven new cembranoids, sarcocrassocolides F-L (1-7), have been isolated from a soft coral Sarcophyton crassocaule. Their structures were determined by extensive spectroscopic analysis. Most new compounds exhibited significant cytotoxic activity against a limited panel of cancer cell lines, and the structure-activity relationship was studied. Compounds 1-7 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein. Compound 4 was also found to effectively reduce the level of COX-2 protein.  相似文献   

Menelloides C and D, new sesquiterpenoids from the Gorgonian coral Menella sp     

Kao SY  Su JH  Hwang TL  Sheu JH  Wen ZH  Wu YC  Sung PJ 《Marine drugs》2011,9(9):1534-1542

Two new metabolites, including a lindenane-type sesquiterpenoid, menelloide C (1), and a germacrane-type sesquiterpenoid, menelloide D (2), were isolated from a Formosan gorgonian coral identified as Menella sp. The structures of 1 and 2 were established by spectroscopic methods and 2 displayed a weak inhibitory effect on the release of elastase by human neutrophils.  相似文献   

Bioactive Cembranoids,Sarcocrassocolides P–R,from the Dongsha Atoll Soft Coral Sarcophyton crassocaule     

Wan-Yu Lin  Bo-Wei Chen  Chiung-Yao Huang  Zhi-Hong Wen  Ping-Jyun Sung  Jui-Hsin Su  Chang-Feng Dai  Jyh-Horng Sheu 《Marine drugs》2014,12(2):840-850

New cembranoids, sarcocrassocolides P–R (1–3) and four known compounds (4–7) were isolated from the soft coral Sarcophyton crassocaule. The structures of the metabolites were determined by extensive spectroscopic analysis. Compounds 3–5 and 7 were shown to exhibit cytotoxicity toward a limited panel of cancer cell lines and all compounds 1–7 displayed potent in vitro anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells by inhibiting the expression of inducible nitric oxide synthase (iNOS) protein. Compound 7 also showed significant activity in reducing the accumulation of cyclooxygenase-2 (COX-2) protein in the same macrophage cells.  相似文献   

Oxygenated Eremophilane- and Neolemnane-Derived Sesquiterpenoids from the Soft Coral Lemnalia philippinensis     

Yun-Jie Xio  Jui-Hsin Su  Yen-Ju Tseng  Bo-Wei Chen  Wangta Liu  Jyh-Horng Sheu 《Marine drugs》2014,12(8):4495-4503

Five sesquiterpene-related metabolites (1–5), including two new eremophilane-type compounds, philippinlins C and D (1 and 2) and a 4,5-seconeolemnane philippinlin E (3), were isolated from the organic extract of a Taiwanese soft coral Lemnalia philippinensis. The structures of the new metabolites were determined on the basis of extensive spectroscopic analysis and by comparison of NMR data with those of related metabolites. Compound 3 was suggested to be derived from the neolemnane skeleton.  相似文献   

Antimicrobial, dehydroascorbate reductase, and monodehydroascorbate reductase activities of defensin from sweet potato [Ipomoea batatas (L.) Lam. 'Tainong 57'] storage roots     

Huang GJ  Lai HC  Chang YS  Sheu MJ  Lu TL  Huang SS  Lin YH 《Journal of agricultural and food chemistry》2008,56(9):2989-2995

A cDNA encoding a small cysteine-rich protein designated defensin (SPD1) was isolated from sweet potato storage roots. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that SPD1 is a member of the plant defensin family. Recombinant SPD1 protein overproduced in Escherichia coli was purified by Ni (2+)-chelated affinity chromatography. A recombinant protein from the storage root cDNA clone effectively inhibited the trypsin activity in a dose-dependent manner. Both the corresponding mRNA and protein level were found to be highest in the storage roots, followed by sprout. SPD1 reduced dehydroascorbate (DHA) in the presence of glutathione to regenerate l-ascorbic acid (AsA). However, without glutathione, SPD1 has very low DHA reductase activity, and AsA was oxidized by AsA oxidase to generate monodehydroascorbate (MDA) free radical. MDA was also reduced by SPD1 to AsA in the presence of NADH, mimicking the MDA reductase catalyzed reaction. These data suggest that SPD1 has both DHA reductase and MDA reductase activities. SPD1 was also shown to inhibit the growth of both fungi and bacteria. SPD1 is apparently the first reported plant defensin exhibiting DHA and MDA activities in vitro.  相似文献   

Analysis of patulin in apple juice by diphasic dialysis extraction with in situ acylation and mass spectrometric determination.     

F Sheu  Y T Shyu 《Journal of agricultural and food chemistry》1999,47(7):2711-2714

A procedure combining diphasic dialysis extraction with in situ acylation and gas chromatography/mass spectrometry (GC/MS) determination was developed for detection and quantification of the mycotoxin patulin in apple juice. Apple juice samples spiked with 4-N,N-dimethylaminopyridine were dialyzed using methane chloride and acetic anhydride inside dialysis tubing. Patulin was derivatized into its acetate and collected in the tubing after diphasic dialysis and was directly determined using GC/MS with the selective ion monitoring mode without further concentration and cleanup steps. Quantification was carried out by a calibration curve with an internal standard of correlation. The appropriate parameters of both dialysis and derivatization were examined. The linear range of the calibration curve was found to be 10-250 microg/L for patulin, and the limit of quantification was 10 microg/L. Levels of patulin ranging from 0 to 107.2 microg/L with 77-109% recovery were found in 10 apple samples. The technique combining diphasic dialysis extraction and acylation was demonstrated and showed potential for other applications.  相似文献   

Rapid extraction of epidermis chlorophyll of moso bamboo (Phyllostachys pubescens) culm using ultrasonics     

Shang-Tzen Chang  Sheng-Yang Wang  Jyh-Horng Wu 《Journal of Wood Science》1998,44(1):78-80

The feasibility of applying ultrasonics to the chlorophyll extraction of bamboo culm was evaluated to establish an efficient reliable method for chlorophyll extraction from the epidermis of bamboo culm. The results demonstrate that extraction of epidermis chlorophyll from bamboo culm with acetone by ultrasonics for 3 min is a rapid, reliable method.  相似文献