Plant secondary metabolites regulating behaviour of zoospores of the phytopathogenic fungus Aphanomyces cochlioides     

Satoshi Tahara  Masanori Mizutani  Tomohiko Takayama  Kaori Ohkawa 《Pest management science》1999,55(2):209-211

A number of compounds isolated from various plant species were tested for their ability to affect the mobility of zoospores of the fungus Aphanomyces cochlioides which causes root rot in spinach (Spinacia oleracea). Compounds may act as attractants, repellents or stimulants of zoosphore movement or they may halt movement by causing the spore to clump and settle. Bioassay revealed compounds with these methods of action, as well as some which acted directly on the fungus. ©1999 Society of Chemical Industry  相似文献   

Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture,Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism     

Yuma SAHASHI  Masaaki OSHIMA  Junjiro YAMAGISHI  Chieko MURAMATSU  Kaori SHIMIZU  Yasuo INOSHIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1730

Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes.  相似文献   

Potential application of simple easy-to-use insertion-deletion (InDel) markers in citrus cultivar identification     

Takahiro Noda  Kaoru Daiou  Takashi Mihara  Yukio Nagano 《Breeding Science》2021,71(5):601

We previously developed insertion-deletion (InDel) markers that distinguish three genotypes (two homozygous and one heterozygous) of diverse citrus cultivars. These InDel markers were codominant and could be clearly detected by using simple agarose gel electrophoresis. We sought to establish a method for cultivar identification using these 28 InDel markers to genotype 31 citrus cultivars. The results revealed that a minimum of 6 markers were required to identify individuals using the three-genotype classification method. Furthermore, we found that a simple method for distinguishing between two genotypes (homozygous and heterozygous) could be used to identify individuals using a minimum of 7 markers. Our findings provide a basis for the development of simple and rapid citrus cultivar identification methods.  相似文献   

The characterization of autoallotetraploid hybrids between Iris ensata Thunb. and I. laevigata Fisch.   总被引：1，自引：0，他引：1  

T. Yabuya  T. Noda 《Euphytica》1998,103(3):325-328

The characteristics of autoallotetraploid hybrids obtained from the cross between Iris ensata cv. Raspberry Rimmed (4X) and amphidiploids of I. laevigata × I. ensata were examined and compared with those of their parents. The color of inner and outer perianths in the autoallotetraploids were bluish purple and similar to those of the amphidiploid parent. However, the autoallotetraploids exhibited low pollen fertility. In addition, the autoallotetraploids were characterized by 17 or 19 anthocyanins and had high resemblance to their parents in the anthocyanin expression. Among these anthocyanins, malvidin 3RGac5G and petunidin 3RGac5G were regarded as major anthocyanins in the autoallotetraploids and their parents, but the differences in the ratios of malvidin 3RGac5G:petunidin 3RGac5G between the autoallotetraploids and their parents were ca. 2:1 for the former and ca. 1:1 for the latter. No viable hybrid seeds were obtained from the reciprocal crosses between I. ensata (2X and 4X) and the autoallotetraploids. Finally, the interspecific cross-breeding of I. ensata using the autoallotetraploids is discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Regulation of biosynthesis,perception, and functions of strigolactones for promoting arbuscular mycorrhizal symbiosis and managing root parasitic weeds     

Koichi Yoneyama  Xiaonan Xie  Kaori Yoneyama  Takahito Nomura  Ikuo Takahashi  Tadao Asami  Narumi Mori  Kohki Akiyama  Miyuki Kusajima  Hideo Nakashita 《Pest management science》2019,75(9):2353-2359

Strigolactones (SLs) are carotenoid‐derived plant secondary metabolites that play important roles in various aspects of plant growth and development as plant hormones, and in rhizosphere communications with symbiotic microbes and also root parasitic weeds. Therefore, sophisticated regulation of the biosynthesis, perception and functions of SLs is expected to promote symbiosis of beneficial microbes including arbuscular mycorrhizal (AM) fungi and also to retard parasitism by devastating root parasitic weeds. We have developed SL mimics with different skeletons, SL biosynthesis inhibitors acting at different biosynthetic steps, SL perception inhibitors that covalently bind to the SL receptor D14, and SL function inhibitors that bind to the serine residue at the catalytic site. In greenhouse pot tests, TIS108, an azole‐type SL biosynthesis inhibitor effectively reduced numbers of attached root parasites Orobanche minor and Striga hermonthica without affecting their host plants; tomato and rice, respectively. AM colonization resulted in weak but distinctly enhanced plant resistance to pathogens. SL mimics can be used to promote AM symbiosis and to reduce the application rate of systemic‐acquired resistance inducers which are generally phytotoxic to horticultural crops. © 2019 Society of Chemical Industry  相似文献   

Establishment of a canine lens epithelial cell line     

Akira Matsuda  Yuki Shimizu  Teppei Kanda  Akihiro Ohnishi  Noritaka Maeta  Masahiro Miyabe  Kaori Saeki  Yoshiki Itoh 《Canadian journal of veterinary research》2021,85(3):236

Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitro examinations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-β) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts.  相似文献   

Mink enteritis in Japan. II. Epidemiology of the disease     

T Higashihara  H Izawa  M Onuma  H Kodama  T Mikami  H Noda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1981,43(6):929-931

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Acinic cell carcinoma in an African pygmy hedgehog (Atelerix albiventris)     

Fukuzawa R  Fukuzawa K  Abe H  Nagai T  Kameyama K 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):39-42

Abstract: A male African pygmy hedgehog ( Atelerix albiventris ), estimated to be 3 years old, presented with exophthalmos and fixed abduction of the right eye. Radiographic examination revealed a retrobulbar tumor in the right orbital cavity. The mass was surgically resected but recurred 3 months later and the hedgehog died. There was no gross or microscopic evidence of salivary or lacrimal gland involvement of the tumor at surgery or at necropsy. The histopathologic, immunohistochemical, and ultrastructural findings were those of acinic cell carcinoma, the origin of which was unknown. This is the first known case of acinic cell carcinoma in an African hedgehog.  相似文献   

Protectivity of an immunoaffinity-purified 39 kDa capsular protein of avian Pasteurella multocida in mice     

Ali HA  Sawada T  Noda K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1603-1604

A 39 kDa protein of avian Pasteurella multocida strain P-1059 (serovar A:3) was purified from a crude capsular extract by immunoaffinity chromatography by using a ligand of purified mouse monoclonal antibody to the 39 kDa capsular protein of the strain. Protective activity of the purified 39 kDa protein antigen was determined by inoculation of ddY mice twice with 25 or 125 mug of the protein and challenge-exposure with 10 or 50 LD(50) of strains P-1059 or X-73 (serovar A:1). The results showed that the antigen gave high protection (60 to 100%). These results indicated that the 39 kDa protein of avian P. multocida is a cross-protective antigen over serovars A:1 and A:3.  相似文献   

Carbon balance in the life cycle of wood: targeting a timber check dam     

Chihiro Kayo  Ryu Noda  Takanobu Sasaki  Shinya Takaoku 《Journal of Wood Science》2015,61(1):70-80

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