Reduced Efficacy of Ivermectin Treatments in Gastrointestinal Nematode Infections of Grazing Cattle in Japan     

Noboru KUDO  Toshiro YOSHIOKA  Yasushi WATANABE  Yuki TERAZONO  Shino TAKENOUCHI  Takuto DONOMOTO  Kensuke NAKAJIMA  Kaori HITOSUGI  Ryusuke TSUKADA  Hiromi IKADAI  Takashi OYAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1487-1491

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Decreased Gene Expressions of Insulin Signal Molecules in Canine Hyperadrenocorticism     

Satoshi NOZAWA  Hitomi ODA  Ran AKIYAMA  Kaori UEDA  Kaori SAEKI  Saori SHONO  Natsuki MARUYAMA  Atsuki MURATA  Hiroyuki TAZAKI  Akihiro MORI  Yutaka MOMOTA  Daigo AZAKAMI  Toshinori SAKO  Katsumi ISHIOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1177-1182

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Multiple high-throughput analyses monitor the response of E. coli to perturbations     

Ishii N  Nakahigashi K  Baba T  Robert M  Soga T  Kanai A  Hirasawa T  Naba M  Hirai K  Hoque A  Ho PY  Kakazu Y  Sugawara K  Igarashi S  Harada S  Masuda T  Sugiyama N  Togashi T  Hasegawa M  Takai Y  Yugi K  Arakawa K  Iwata N  Toya Y  Nakayama Y  Nishioka T  Shimizu K  Mori H  Tomita M 《Science (New York, N.Y.)》2007,316(5824):593-597

Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.  相似文献   

Repression of the transcription factor Th-POK by Runx complexes in cytotoxic T cell development   总被引：1，自引：0，他引：1  

Setoguchi R  Tachibana M  Naoe Y  Muroi S  Akiyama K  Tezuka C  Okuda T  Taniuchi I 《Science (New York, N.Y.)》2008,319(5864):822-825

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Validation of a newly generated oxytocin antibody for enzyme-linked immunosorbent assays     

Kaori MURATA  Miho NAGASAWA  Tatsushi ONAKA  Ken-ichi TAKEYAMA  Takefumi KIKUSUI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):478

The biological and psychological significance of oxytocin is increasingly recognized; however, reliable assays of oxytocin in biological samples have not been developed. We raised a new oxytocin polyclonal rabbit antibody against synthetic oxytocin. The affinity of antibodies to oxytocin was examined by a radio-immunoassay and compared with that of a previously validated antibody. One antibody showed affinity for oxytocin in the radio-immunoassay. We developed a solid-phase ELISA for oxytocin using this antibody and compared it with existing methods. The newly developed ELISA showed comparable results using urine samples but not using serum samples. These results indicate that the new ELISA is useful for urinary oxytocin; further modifications, such as different extraction methods, are needed for its application to serum oxytocin.  相似文献   

Effect of chloride application and low soil pH on cadmium uptake from soil by plants   总被引：3，自引：0，他引：3  

Hiroyuki HATTORI  Kouki KUNIYASU  Kaori CHIBA  Mitsuo CHINO 《Soil Science and Plant Nutrition》2006,52(1):89-94

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Induction of 2n pollen in tulips by arresting the meiotic process with nitrous oxide gas     

Keiichi Okazaki  Kaori Kurimoto  Ichirou Miyajima  Akira Enami  Hitoshi Mizuochi  Yasu Matsumoto  Hirotaka Ohya 《Euphytica》2005,143(1-2):101-114

Triploid tulips have agronomically desirable traits such as vigorous growth and large flower size, but only a portion of all cultivated tulips is triploid. To apply 2n pollen to polyploid breeding of tulips, the polyploidizing agent, nitrous oxide gas (N₂O), was applied to bulbs. In tulips, meiosis in anthers occurs inside the bulbs from mid- to late-October. When meiosis in anthers (excised from bulbs) reached metaphase I, we treated other bulbs of the same clones with N₂O for 24–48 h. Most of the treated plants produced pollen grains with a wide-ranging or bimodal size distribution, indicating a mixture of n, 2n and aneuploid pollen grains. The use of pollen containing a relatively high proportion of giant pollen grains tended to yield larger numbers of triploids in the progeny. The number of giant pollen grains could be increased when N₂O-treated pollen grains were suspended in 10% sucrose and then sieved through a nylon mesh. Very few polyploids were observed in some cross combinations, even those involving pollen with a relatively high proportion of giant grains. Even so, this low polyploid yield most likely is due to a triploid block, because the capsules obtained in the crosses of the diploid×N₂O-treated plants contained some abnormal seeds, which were mostly triploid. Embryo culture was useful in rescuing abnormal embryos. The present study reveals that 2n pollen can be produced at high frequency using N₂O during tulip breeding.  相似文献   

Barley lines showing prominent high green shoot regeneration in cultures derived from immature embryos     

K. Rikiishi    T. Matsuura    M. Maekawa    K. Noda  K. Takeda 《Plant Breeding》2003,122(2):105-111

Eighty‐four cultivars of barley (Hordeum vulgare) and 95 wild strains (82 of H. spontaneum and 13 of H. agriocrithon) were surveyed for the production of callus, callus growth, and shoot regeneration in cultures derived from immature embryos. All cultivars except for ‘Turkey 381′, induced calli from more than 90% of embryos. On the other hand, the wild lines showed a large variation in the percentage of callus induction from 0 to 100%. Among the cultivars, those with the brittle rachis genotype, bt Bt₂, on chromosome 3H, regenerated shoots with a significantly higher percentage than the cultivars with the Bt bt₂ genotype. Green shoots were produced in a higher ratio (0.84) in the cultivars than in the wild lines (0.52). Among the lines examined,‘Lenins’ regenerated shoots efficiently (90.4%) and produced the highest number of calli with green shoots per embryo (4.77) followed by ‘Golden Promise’ (3.15). Examination of callus growth and shoot regeneration from embryos at different developmental stages revealed that scutellum development affected the quantity and quality of callus and shoot regeneration.  相似文献   

Gene transcription analysis in lesional skin of canine epitheliotropic cutaneous lymphoma using quantitative real-time RT-PCR     

Chimura N  Kondo N  Shibata S  Kimura T  Mori T  Hoshino Y  Murayama N  Nagata M  Ide K  Nishifuji K  Kamishina H  Maeda S 《Veterinary immunology and immunopathology》2011,144(3-4):329-336

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Evaluation of serum and urine 1,5-anhydro-D-glucitol and myo-inositol concentrations in healthy dogs     

Miki Y  Mori A  Hayakawa N  Niki T  Oda H  Saeki K  Sato T  Tazaki H  Ishioka K  Arai T  Sako T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1117-1126

1,5-anhydro-D-glucitol (1,5AG) is a pyranoid polyol compound found in human circulating blood. Myo-inositol (MI) is a stereoisomer of inositol and serves as a precursor of inositol phospholipids. 1,5AG and MI are filtered by the glomerulus and almost completely reabsorbed through the renal tubules. However, under hyperglycemic conditions, reabsorption through the renal tubules is competitively inhibited because the structures of 1,5AG and MI resemble that of glucose. In this study, we investigated the kinetics of serum and urine 1,5AG and MI levels in healthy dogs. We demonstrated that 1,5AG and MI exist in canine serum and urine by gas chromatography-mass spectrometry. Under continuous hyperglycemic conditions, the serum 1,5AG concentration in healthy dogs decreased while the serum MI concentration remained unchanged. Urinary excretion of 1,5AG and MI increased significantly after blood glucose concentrations reached 200 to 220 mg/dl. A significant negative correlation was observed between serum 1,5AG and glucose concentrations during hyperglycemia. However, no significant correlation was observed between serum MI and glucose concentrations. In this study, we demonstrated that serum and urine 1,5AG and MI levels were changed by blood glucose concentrations. The serum 1,5AG concentration was decreased by continuous hyperglycemia. However, the serum MI concentration does not reflect hyperglycemia.  相似文献